Rice leaf diseases pose a significant threat to global food security by reducing crop productivity and causing substantial economic losses. The traditional diagnosis method is manual method, which is low in efficiency, subjective and not suitable for large-scale agricultural monitoring. Despite the advances in automated disease detection using deep learning methods like CNNs, GANs, and transfer learning models, these techniques remain highly computational, not very flexible, and struggle to perform well in different imaging scenarios. Considering these drawbacks, this paper introduces an Explainable Deep Q-Learning based CNN framework which employs CNN-based feature extraction and Deep Q-learning-based adaptive policy optimization for rice leaf disease classification. The proposed model continuously refines the classification actions through reward-based learning, which makes the model more robust in various agricultural imaging environments, in contrast to traditional supervised CNN models that have static classification decisions. The proposed model achieved 98.5% accuracy, 98.52% precision, 98.50% recall, and a 98.51% F1-score, outperforming existing CNN, GAN, reinforcement learning, and transformer-based methods. It also offers a high computational efficiency of 14.2 GFLOPs, 248 MB memory consumption, ~ 48 min of training time, and 6.8 ms inference time per image suitable for resource constrained applications in agriculture. The results demonstrate the effectiveness, scalability, and practical applicability of the proposed framework. The proposed framework performs well on benchmark datasets but more research in the deployment of the edge-devices under different real-world agricultural settings will be investigated in future work.
Why it matches plant phenotyping methodsイネ葉の病害状態を画像から分類する深層学習手法の開発・評価が研究の中心であり、植物の病害表現型を直接推定しているため。
abstractthis paper introduces an Explainable Deep Q-Learning based CNN framework which employs CNN-based feature extraction and Deep Q-learning-based adaptive policy optimization for rice leaf disease classification.
Reproduction assets foundThe paper trains its Deep Q-CNN rice leaf disease classifier on public Kaggle rice leaf image datasets, which are cited with explicit public URLs and qualify as paper-specific phenotyping image inputs. The authors' own derived data/analysis artifacts are only available upon request, so no authors' code or trained modelDataset · publicSoni Gautam. Rice Leaf Bacterial and Fungal Disease Dataset. Kaggle. Available:Open asset ↗Kagglepdf-page:24 lines:1-94Code / dataset availability confirmedCrossref · checked 15 Sept 2026
Precision agriculture is becoming more and more of a challenge that requires the use of intelligent systems that are able to predict stress and prevent yield loss before it is too late. Traditional methods of agricultural surveillance are predominantly reactive with irrigation demands being based on thresholds or individual yield forecasts models that do not represent the intricate spatio-temporal interactions that exist between crop physiology, soil status, and environmental stresses. Besides, the majority of the current practices do not have an autonomous decision-making approach to preventive intervention which leads to inefficient use of water and slows down the response to stress. This paper suggests a cognitive UAV-assisted agro-surveillance system to predict yield vulnerability caused by crop stress and optimize adaptive irrigation with the help of spatio-temporal deep and reinforcement learning. The framework combines UAV-obtained RGB and multispectral and thermal imagery with measurements of soil sensors and meteorological data obtained with the Crop Health and Environmental Stress Dataset. A new GeoSpatio-TRiNet model is used to acquire long-range spatial relationship, time stress development, and diffusion of stresses across agricultural regions. The model predicts the vulnerability trajectories of the stress instead of the direct yield regression, and this allows early detection of yield risk. Such predictions serve to generate a cognitive environmental state of a Soft ActorCritic (SAC) reinforcement learning agent that autonomously computes zone-based irrigation behaviors to reduce the recurrence of stress at the minimum water usage cost. As shown by the results of the experiment, the proposed framework has a stress forecasting accuracy of 96.3% and performs much better than the traditional machine learning, CNN-based, and transformer-based baselines. The system also decreases the predicted yield vulnerability by 46.6 and enhances water-use efficiency by 41.1 as compared to irrigation strategies based on rules. The results confirm the usefulness of spatio-temporal intelligence with predictive control in terms of effectiveness, and the proposed framework is a scalable and sustainable solution to precision agriculture of the next generation.
Why it matches plant phenotyping methodsUAV画像とセンサーデータから作物ストレスの時系列状態および収量脆弱性を推定する計算・センシング手法が研究の中心であり、灌漑制御への応用も技術評価の一部として記述されている。
abstractThe framework combines UAV-obtained RGB and multispectral and thermal imagery with measurements of soil sensors and meteorological data
Reproduction assets foundThe paper uses the public Kaggle Crop Health and Environmental Stress Dataset (UAV RGB/multispectral/thermal imagery plus soil/weather measurements and stress labels) as its phenotyping data source, and the authors provide an explicit public GitHub repository for the analysis code.Dataset · publicThe current research is based on the Crop Health and Environmental Stress Dataset, which is a publicly available
dataset on Kaggle, specially created to help perform a spatio-temporal analysis of crop health in response to changing
environmental and water-stress factors [26].Open asset ↗pdf-raw-page:10 lines:1-62Code · publicturn: Final zone-wise stress predictions 𝐶
𝑡
𝑧, Yield vulnerability trajectories 𝑉𝑡
𝑧, Optimal adaptive irrigation policy
𝜋∗
End Algorithm
Code availability:
The data used to support the findings of this study are included in the article.
Code availability:
The code used in this research work is available in the following link.
https://github.com/replyvenugopal/Cognitive-UAV-Driven-Agro-Surveillance
4. Result and Discussion
The architectural agro-surveillance solution, which is proposed to be executed by UAVs, is executed through a
modular and scalable software framework to guarantee reproducibility and extensibility. The experiments are all
performed in Python as a main programming languageOpen asset ↗github.com/replyvenugopal/Cognitive-UAV-Driven-Agro-Surveillancepdf-raw-page:24 lines:1-55Code / dataset availability confirmedOpenAlex · Crossref · checked 15 Sept 2026
Abstract Heat stress causes ultrastructural damage in pollen grains, leading to reduced pollen germination, pollen size and shortened pollen tube length, ultimately lowering seed set and yield. This study presents a high-throughput phenotyping framework that integrates controlled-environment pollen germination assays with deep learning–based object detection for rapid, accurate, and scalable evaluation of reproductive heat tolerance in soybean breeding programs. Sixteen soybean genotypes were grown under controlled environments at optimal (28/18°C; day/night) and high temperature (38/28°C; day/night) regimes during flowering. In vitro pollen germination was quantified using six YOLO (You Only Look Once) object-detection architectures (YOLOv7–YOLOv12) to identify the best-performing model for automated analysis. Among the tested object-detection architectures, YOLOv9 achieved the best overall performance for detecting germinated and non-germinated pollen grains in complex images. High temperature significantly reduced mean pollen germination from an average of 40% under optimal conditions to an average of 21% under heat stress (P < 0.05), with a significant genotype × growth temperature interaction. Invitro incubation temperatures ranging from 10 °C to 45 °C produced a clear thermal response; however, no significant genotype × incubation temperature interaction was detected within either growth temperature regime. Although photosynthetic and physiological traits were measured exploring their relationship with pollen germination, their transient and complex response limited their reliability for predicting reproductive performance. The automated pipeline substantially reduced the time required to evaluate pollen germination. The pipeline processed nearly 5,000 images in approximately one hour, substantially increasing throughput and reducing reliance on manual counting. The findings demonstrate that pollen germination is a promising proxy trait for screening reproductive heat tolerance in soybean. Combining controlled environment phenotyping with YOLO-based object detection enabled efficient, accurate, and scalable pollen analysis, and represents the central methodological advance of this study. YOLOv9 performed best among the tested architectures, although discrepancies from manual counts in some images indicate that additional validation is needed. The weak associations with vegetative physiological traits further support the value of direct pollen-based phenotyping.
Why it matches plant phenotyping methods深層学習による花粉画像解析を中心に、花粉発芽という生殖形質を高速・自動測定するハイスループット表現型解析フレームワークを開発・比較・検証している。
abstractThis study presents a high-throughput phenotyping framework that integrates controlled-environment pollen germination assays with deep learning–based object detection for rapid, accurate, and scalable evaluation of reproductive heat tolerance in soybean breeding programs.
Reproduction assets foundThe authors state that all data supporting the study, including annotated pollen germination images, computational and statistical codes, and analysis tools, were deposited in Zenodo with a public DOI. This is a paper-specific, publicly actionable asset. LabelMe and Ultralytics YOLO are generic third-party tools, not作者Dataset · publicCommission.
Data availability
All data supporting the findings of this study, including annotated images, computational and
statistical codes, and analysis tools, have been deposited in the Zenodo data repository. Additional
data will be made available upon reasonable request following acceptance of the manuscript.
Repository: https://doi.org/10.5281/zenodo.21685593
Ethics approval and consent to participate
Not applicable
Consent for publication
Not applicable
Competing Interests
Authors declared no competing interests
References
1. FAOSTAT: Crops and livestock products: soybean production data. https://www.fao.org/faostat/
(2022). Accessed 15 Feb 2026.
2. Patel D, Franklin KA. TemperaturOpen asset ↗Zenodo · 10.5281/zenodo.21685593pdf-raw-page:28 lines:1-34Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Abstract Crop diseases pose significant challenges to productivity in resource-constrained settings, often remaining undiagnosed when diagnostic tools and infrastructure are either non-existent or inadequate. Current crop disease diagnosis relies on manual inspection methods that are labor-intensive, prone to error, and incapable of delivering real-time or region-specific insights in the process. Such limitations call for developing advanced diagnostic systems that are scalable and efficient in resource-constrained settings. This research introduced a comprehensive multi-spectral imaging and machine learning framework that can easily revolutionize the disease diagnosis and management inside the low-resource farming communities. Built within its core is the 3D Spectral-Spatial Convolutional Neural Network (3D SSCNN) that extracts high-resolution spectral-spatial features from hyperspectral image cubes. The accuracy achieved is around ~ 95% within 0.3 s per sample. Fed-DiagNet has provided support for distributed training that enables scalability and also data privacy to enhance the accuracy of regional models at approximately 92% as well as reduces training by almost 40%. Temporal disease progression modeling is enabled by Temporal Progression LSTM that provides dynamic trends with 90% accuracy up to a horizon of 10 days. This means that in addition to integrating disparate data sources-including hyperspectral imagery, environmental data, and pest observations-MTAN achieves an almost ~ 93% stress identification accuracy. Lastly, an RL-FO system tailors its treatment recommendations to local conditions so as to optimize for yield improvement and cost-effectiveness. With the proposed system, diagnostic precision increases to ~ 94%, and it is manifested in real-time efficiency while supporting scalability with actionable insights to empower farmers to mitigate crop losses and augment food security across several scenarios.
Why it matches plant phenotyping methods植物病害の状態をマルチスペクトル画像から推定する画像・機械学習フレームワークの開発が研究の中心であり、植物フェノタイピング手法に該当する。
abstractThis research introduced a comprehensive multi-spectral imaging and machine learning framework that can easily revolutionize the disease diagnosis and management inside the low-resource farming communities.
Reproduction assets foundThe paper's Data Availability statement points to two public repositories containing the data analyzed: a Kaggle PlantVillage dataset and a GitHub hyperspectral datasets repository. No author code or models are explicitly deposited.Dataset · publicAll data analyzed during this study are available in the Kaggle and Github repository, in the links https://www.kaggle.com/datasets/rohithaaiswarya/plant-village and https://github.com/antmedellin/HyperspectralDatasets .Open asset ↗Kaggle · rohithaaiswarya/plant-villagelines:563-583Dataset · publicAll data analyzed during this study are available in the Kaggle and Github repository, in the links https://www.kaggle.com/datasets/rohithaaiswarya/plant-village and https://github.com/antmedellin/HyperspectralDatasets .Open asset ↗GitHub · antmedellin/HyperspectralDatasetslines:563-583Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The tomato plant is considered one of the most important crops in the world, yet it is vulnerable to various diseases that affect crop quality and agricultural productivity. These challenges have driven the need for an efficient and intelligent plant disease detection system. With the development of computer vision and artificial intelligence, this proposed methodology based on deep learning for tomato leaf diseases has been presented. Two public datasets: Taiwan DS with nine classes and Tomato Leaf Diseases Detection Computer Vision Dataset (TLDDCV DS) with seven classes have been used to test this system. This system begins with plant image processing, which includes gamma correction and bilateral filtering, to enhance image quality and clarity while preserving key disease features. Then, a genetic metaheuristic algorithm was used to automatically select the most significant hyperparameters, further optimizing both processing time and accuracy. After that, the tomato leaf disease detection applies the You Only Look Once version 11 Nano (YOLOv11n) model. The YOLOv11n backbone is edited through a Data-efficient Image Transformer (DeiT) to improve the system's capacity for learning global contextual information and long-range dependencies. Experimental results demonstrate that the proposed system outperforms existing methods. It achieved an average mAP@50 of 97.8%, mAP@50-95 of 93.4%, precision of 97.3%, recall of 93.8%, and F1-score of 95.5% on the Taiwan dataset. Additionally, it achieved an average mAP@50 of 87%, mAP@50-95 of 48%, precision of 83.9%, recall of 70.3%, and F1-score of 76.4% on the TLDDCV dataset. These results demonstrate the generalizability and effectiveness of the proposed system in real-world agricultural situations.
Why it matches plant phenotyping methodsトマト葉の病徴を画像から検出・分類する深層学習手法の開発と2データセットでの性能評価が研究の中心であり、植物病害状態のフェノタイピングに該当する。
abstractthe tomato leaf disease detection applies the You Only Look Once version 11 Nano (YOLOv11n) model.
Reproduction assets foundThe paper uses two public Roboflow tomato leaf disease image datasets and states its source code is publicly available on Zenodo, all with explicit availability statements and URLs.Dataset · publicThe first dataset is the Taiwan dataset, which can be found at the following link: (https://universe.roboflow.com/bryan-b56jm/tomato-leaf-disease-ssoha).Open asset ↗tomato-leaf-disease-ssohalines:317-328Dataset · publicThe second dataset is the TLDDCV dataset, which can be found at the following link: (https://universe.roboflow.com/sylhet-agricultural-university/tomato-leaf-diseases-detect)Open asset ↗tomato-leaf-diseases-detectlines:317-328Code · publicThe source code of the proposed framework, including the implementation of the proposed methodology and experimental setup, is publicly available in the Zenodo repository: https://doi.org/10.5281/zenodo.20777853 .Open asset ↗Zenodo · 10.5281/zenodo.20777853lines:317-328Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Field / plotLeafClassificationDisease symptoms / severity
Early and reliable diagnosis of hibiscus leaf diseases is critical to protect horticultural yield. Yet, it remains challenging under real-time field conditions where uncontrolled lighting, clutter, and the non-contiguous nature of pathological symptoms blur diagnostic cues. To address these challenges, we introduce CNN-FusionViT-GNN. This explainable hybrid multi-branch framework synergizes the fine-grained texture extraction of a DenseNet201 backbone, the global contextual modeling of a Vision Transformer (ViT), and the relational reasoning of a Graph Neural Network (GNN). The model is trained and validated on 'Hibiscus,' a curated field dataset of 1165 images from Bangladesh, which is strategically augmented to 8000 samples for robust training following a strict train-validation-test split. The proposed framework achieves a state-of-the-art accuracy of 98.33% with a macro F1-score of 0.98. The framework's generalization is confirmed through high performance on external datasets: 98.78% accuracy on the 52-class Plant City dataset and 83.88% on the 10-class Tomato Leaf Disease dataset, while maintaining a rapid inference time of 10-45 ms. Furthermore, a multi-faceted Explainable AI (XAI) audit using LIME, Grad-CAM++, ViT Attention Maps, and Occlusion Sensitivity validates that the model's decisions are driven by biologically meaningful symptom patterns rather than background artifacts. This study establishes a computationally efficient, transparent, and robust pathway for automated disease diagnosis in precision agriculture.
Why it matches plant phenotyping methodsハイビスカス葉の病徴を画像から分類するCNN-ViT-GNN手法を開発し、複数データセットで性能検証しているため、植物病害表現型の取得・推定が中心である。
abstractwe introduce CNN-FusionViT-GNN. This explainable hybrid multi-branch framework synergizes the fine-grained texture extraction of a DenseNet201 backbone, the global contextual modeling of a Vision Transformer (ViT), and the relational reasoning of a Graph Neural Network (GNN).
Reproduction assets foundThe paper's primary Hibiscus leaf disease image dataset is publicly deposited on Mendeley Data, and the external Tomato Leaf Disease dataset used for validation is also publicly available on Mendeley Data. No author analysis code or trained model checkpoints are reported.Dataset · publicThe primary dataset generated and analyzed during the current study,“Hibiscus Leaf Diseases Classification Dataset,”is publicly available in Mendeley Data 7 .Open asset ↗Mendeley Datalines:307-347Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Accurate identification of rice diseases from field images is critical for crop health monitoring and sustainable agriculture, particularly in low-resource environments. However, most deep learning approaches depend on large-scale labeled datasets and pretrained backbones, limiting their applicability to rare or emerging diseases. In this work, we formulate a domain-specific prototype-based few-shot framework that avoids pretrained visual backbones and treats rice disease recognition as structured matching over a pathogen-aware class graph. The individual components, including wavelet-scattering features, optimal transport, semantic prototype fusion, and transductive refinement, are established techniques; the contribution lies in their coupled use within a disease-taxonomy-guided few-shot matching process. This design combines fixed visual descriptors, root-to-leaf prototype matching, class symptom descriptors, and confidence-gated refinement to support rice disease recognition under limited labeled data. We evaluate the model on two publicly available rice disease datasets-the Philippines Rice Diseases and Roboflow Rice-under 1-shot and 5-shot classification settings. In in-domain experiments, our approach achieves up to 95.8% accuracy and 94.9% macro-F1 on the Philippines dataset, consistently outperforming a diverse set of baselines including CNN-from-scratch, ResNet-18-from-scratch, Matching Networks, MAML, ProtoNet, RelationNet, SimpleShot, FEAT, and a flat optimal-transport variant. In cross-domain evaluation, the model demonstrates strong generalization capability, attaining up to 91.7% accuracy and 90.6% macro-F1 when transferring across datasets. An ablation study further confirms the consistent contribution of hierarchical structure, semantic fusion, and transductive refinement to performance gains. These results demonstrate that the proposed framework delivers highly accurate, robust, and data-efficient disease recognition, making it well-suited for real-world agricultural deployment under limited supervision.
Why it matches plant phenotyping methodsイネの病害を圃場画像から認識する手法を開発・評価しており、植物の病害状態を画像から推定する方法が研究の中心である。
abstractwe formulate a domain-specific prototype-based few-shot framework
Reproduction assets foundThe paper evaluates its few-shot rice disease recognition framework on two publicly available rice disease image datasets, with explicit public URLs in the Data Availability statement and dataset description sections. No author analysis code or trained model checkpoints are disclosed.Dataset · publicThe datasets used in this study are publicly available: Philippines Rice Diseases dataset (https://www.kaggle.com/
datasets/shrupyag001/philippines-rice-diseases)Open asset ↗Kaggle · shrupyag001/philippines-rice-diseasespdf-page:19 lines:1-81Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Plant diseases significantly affect agricultural productivity and global food security, while accurate disease identification remains challenging because of uncertain and overlapping visual symptoms in leaf images. Existing deep learning approaches often require large annotated datasets and suffer from limited interpretability in practical agricultural environments. This study presents a multi-parameter improved fuzzy soft set-based framework for plant disease classification using tomato leaf images from the PlantVillage dataset. The objective is to develop an interpretable and reliable classification model capable of handling uncertainty in plant disease patterns through feature-driven fuzzy similarity analysis. The methodology integrates image preprocessing, color and texture feature extraction, variance-based feature weighting, prototype generation using K-means clustering, and fuzzy similarity computation using Mahalanobis distance and Gaussian membership functions. RGB, HSV, and Gray-Level Co-occurrence Matrix (GLCM) features are extracted from standardized leaf images and evaluated within an improved fuzzy soft classification framework. Performance comparison is carried out using machine learning models including Support Vector Machine (SVM), Random Forest (RF), Linear Discriminant Analysis (LDA), and Naive Bayes (NB) implemented in Python using Scikit-learn libraries. Experimental simulation results demonstrate that the proposed framework achieves competitive classification performance while preserving interpretability and robustness under uncertain feature distributions. Performance evaluation is conducted through accuracy analysis, ROC-AUC curves, confusion matrices, ablation studies, and Wilcoxon Signed-Rank statistical testing. The proposed Improved Fuzzy Soft model achieved an accuracy of 88.57% which is less than LDA (94.92%), Random Forest (97.78%) and SVM (97.94%) classifiers. However, in the cross data set validation, the proposed Improved Fuzzy Soft model achieved an accuracy of 67.35% which is greater than LDA (51.02%), Random Forest (51.02%) and SVM (55.10%) classifiers. Statistical validation using the Wilcoxon Signed-Rank Test produced a p-value of [Formula: see text], confirming that the performance difference between the Improved Fuzzy Soft framework and the Random Forest classifier is statistically significant under the current experimental setting.
Why it matches plant phenotyping methodsトマト葉画像から植物病害状態を推定する解釈可能な画像解析・分類フレームワークを開発し、複数モデル、交差データセット検証、アブレーション、統計検定で評価しており、病害表現型の取得・抽出手法が中心である。
abstractThis study presents a multi-parameter improved fuzzy soft set-based framework for plant disease classification using tomato leaf images from the PlantVillage dataset.
Reproduction assets foundThe paper uses public tomato leaf image datasets (PlantVillage and PlantDoc from Kaggle) as phenotyping inputs and states the authors' Improved Fuzzy Soft Framework implementation is publicly available on Zenodo with source code and reproduction instructions.Dataset · publicThe dataset analyzed during the current study are available in the repository:
https://www.kaggle.com/datasets/abdallahalidev/plantvillage-datasetOpen asset ↗kaggle.com/datasets/abdallahalidev/plantvillage-datasetpdf-page:24 lines:1-75Code · publicThe implementation of the proposed Improved Fuzzy Soft Framework is publicly available through the Zenodo repository:
https://doi.org/10.5281/zenodo.20570546
The repository contains the source code, documentation, and instructions required to reproduce the experiments reported in
this study.Open asset ↗zenodo · 10.5281/zenodo.20570546pdf-page:25 lines:1-74Code / dataset availability confirmedOpenAlex · Europe PMC · checked 14 Sept 2026
Yellow rust (YR) is a major threat to both bread and durum wheat production, often causing substantial yield losses. Conventional visual scoring of YR severity, while widely adopted, is labor-intensive, time-consuming, and prone to human error. In this study, we evaluated the predictability (PA), defined as the correlation between predicted and observed values, using genomic and phenomic data for YR severity under multiple prediction scenarios in two biparental wheat populations (bread and durum). YR scoring was conducted on two dates, with YR severity visually assessed while unmanned aerial vehicle (UAV)-based high-throughput phenotyping (HTP) data were collected using a multispectral camera. HTP data were processed to extract spectral wavelengths and vegetation indices (VIs), and all lines were also genotyped using SNP arrays. We tested a diverse set of models, including parametric, machine learning, and deep learning approaches. PA increased markedly when HTP-derived data were used compared with genomic markers alone. For example, support vector regression (SVR) improved from 0.35 (markers only) to 0.87 (wavelengths only). However, integrating genomic and phenomic data did not yield further improvements, as models often plateaued when using HTP-derived features alone. Cross-crop prediction demonstrated promising generalization across bread and durum wheat, achieving PA values up to 0.83. For this last task, best linear unbiased prediction (BLUP) and multilayer perception (MLP) consistently provided robust performance across scenarios. These findings highlight the strong potential of UAV-based HTP for rapid, scalable, and accurate prediction of YR severity in wheat. While genomics retains broad utility for breeding, the practical integration of phenomics and AI-driven prediction pipelines will ultimately depend on breeding program strategies, resources, and objectives.
Why it matches plant phenotyping methodsUAV multispectral HTPによる小麦黄さび病重症度の推定と、複数の予測モデルの比較・検証が研究の中心であり、植物病害状態を直接推定する実質的なフェノタイピング手法研究である。
abstractHTP data were processed to extract spectral wavelengths and vegetation indices (VIs)
Reproduction assets foundThe article's Data Availability statement deposits the datasets generated and analyzed in this study (yellow rust phenotyping with UAV spectral data and genomic markers in bread and durum wheat) in the CIMMYT repository under DOI 10.71682/10549375, which is an allowed URL. No author analysis code or trained model is avDataset · publicand scalable strategy for YR assessment in wheat breeding.
Funding
The authors gratefully acknowledge financial support from the Government of Mexico through
the “MasAgro – Cultivos para México” initiative.
Data Availability
The datasets generated and/or analyzed during the current study are available in the CIMMYT
repository: https://doi.org/10.71682/10549375.Acknowledgements
We are deeply grateful to Julio Huerta-Espino for his guidance and support throughout all stages
of this manuscript. We also thank Hedilberto Velásquez Miranda for his valuable assistance with
rust visual score phenotyping, and Neftalí Cruz Pérez for his dedicated support in trial sowing and
field management.
Conflict Open asset ↗10.71682/10549375pdf-raw-page:30 lines:1-37Code / dataset availability confirmedOpenAlex · Europe PMC · checked 5 Sept 2026
Orthoimagery derived from unmanned aerial vehicles (UAVs) has become a valuable data source for crop-growth monitoring. Individual plant-level (IPL) information enables high-throughput analyses by capturing plant-to-plant variability within fields. However, reliable IPL-based analysis requires accurate extraction of plant-specific regions, which remains challenging in soybean cultivation due to weed interference and canopy overlap. This study proposed an automatic preprocessing framework for IPL soybean growth monitoring that integrates deep-learning-based semantic segmentation with a furrow-guided region of interest (ROI) generation strategy using UAV imagery. A segmentation model was developed using combinations of RGB and multispectral orthoimagery, and a furrow line detection algorithm was designed to generate IPL ROIs aligned with crop rows. The ensemble model combining U-Net, DeepLabV3+, and SegFormer achieved the most stable performance (F1-score up to 0.94 and IoU up to 0.89). The furrow-guided ROI generation algorithm also accurately estimated crop counts, showing strong agreement with manual observations (R² = 0.90 and RMSE = 6.35). The generated IPL ROIs enabled accurate quantification of growth-related features, with strong agreement between automatically generated and manually delineated ROIs (R² > 0.90). Overall, the proposed preprocessing framework provides a practical and scalable solution for UAV-based high-throughput phenotyping in soybean and other ridge-based cropping systems.
Why it matches plant phenotyping methodsUAV画像から個体単位の植物領域を抽出し、成長形質を定量化する前処理・セグメンテーション手法が研究の中心であるため。
abstractThis study proposed an automatic preprocessing framework for IPL soybean growth monitoring that integrates deep-learning-based semantic segmentation with a furrow-guided region of interest (ROI) generation strategy using UAV imagery.
Reproduction assets foundThe authors state that the complete implementation of their IPL soybean preprocessing pipeline (semantic segmentation + furrow line detection) is publicly available on Zenodo. The annotated sample dataset, however, is only available upon request from the corresponding author, so it is not a public asset.Code · publicThe complete implementation of
this pipeline is publicly available at https://doi.org/10.5281/zenodo.21095307.Open asset ↗zenodo · 10.5281/zenodo.21095307pdf-page:7 lines:1-62Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
To address this challenge, we propose StyleGAN3-T, the translation-equivariant alias-free variant of StyleGAN3, as a generative framework for producing high-fidelity synthetic plant disease images, integrated with a hybrid Swin Transformer-ResNet50 classifier for precise recognition. Accurate detection of plant leaf diseases is essential for sustainable agriculture and early intervention. However, deep learning models often struggle with small, imbalanced datasets that limit generalization and robustness. To address this challenge, we propose StyleGAN3-T, a novel alias-free generative framework for producing high-fidelity synthetic plant disease images, integrated with a hybrid Swin Transformer-ResNet50 classifier for precise recognition. The proposed approach ensures translation-equivariant, artifact-free image synthesis and enhanced feature diversity. A balanced dataset of 18,000 images was developed by combining real and StyleGAN3-T-generated samples. In pooled GAN benchmarking, StyleGAN2-ADA achieved the strongest generative-quality metrics, whereas StyleGAN3-T was selected as the preferred augmentation model because its alias-free synthesis and spatial consistency yielded superior downstream classification performance in the proposed pipeline.
Why it matches plant phenotyping methods植物病害画像を合成・認識する画像解析手法が研究の中心であり、植物の病害状態を画像から推定するフェノタイピング手法に該当する。
abstractwe propose StyleGAN3-T, a novel alias-free generative framework for producing high-fidelity synthetic plant disease images, integrated with a hybrid Swin Transformer-ResNet50 classifier for precise recognition.
Reproduction assets foundThe paper's grape leaf disease image inputs are two publicly available Kaggle datasets explicitly named in the Data Availability statement. No author code, models, or synthetic dataset deposit is provided; other processed data is request-only.Dataset · publictechnical guidance. Y.L. and A.W. supervised the study, provided critical revisions, and contributed to the interpretation of results. All authors reviewed and approved the final manuscript.
Data availability
The datasets analyzed during the current study are publicly available from Kaggle: Grapevine Disease Dataset (Original) (https://www.kaggle.com/datasets/rm1000/grape-disease-dataset-original; accessed 13 March 2026; license: MIT) and Grape Leaf Disease 4 Class (https://www.kaggle.com/datasets/jawadulkarim117/grape-leaf-disease-4-class; accessed 13 March 2026; license: CC0: Public Domain). Additional processed metadata, label-harmonization records, dataset split definitions, and other daOpen asset ↗Kaggle · rm1000/grape-disease-dataset-originallines:549-576Dataset · publicthors reviewed and approved the final manuscript.
Data availability
The datasets analyzed during the current study are publicly available from Kaggle: Grapevine Disease Dataset (Original) (https://www.kaggle.com/datasets/rm1000/grape-disease-dataset-original; accessed 13 March 2026; license: MIT) and Grape Leaf Disease 4 Class (https://www.kaggle.com/datasets/jawadulkarim117/grape-leaf-disease-4-class; accessed 13 March 2026; license: CC0: Public Domain). Additional processed metadata, label-harmonization records, dataset split definitions, and other data used and/or analyzed during the current study are available from the corresponding author on reasonable request.
Declarations
Competing inOpen asset ↗Kaggle · jawadulkarim117/grape-leaf-disease-4-classlines:549-576Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Timely identification of crop diseases is imperative in precision agriculture to intervene at the right time and maximise yield sustainability. Despite achieving high accuracy, deep learning models are ineffective for rare plant disease classes, mainly due to severe data imbalance and insufficient training samples. Currently, most generative augmentation methods are designed to enhance either visual realism or data diversity, while ignoring methods that are sensitive to early-stage diseases or that control disease progression. In this paper, we propose RareAgriDetectAI, which comprises RareSimGAN, a generative deep learning framework for synthesising images of rare diseases, and a latent traversal mechanism that collaboratively visualises disease progression with increasing severity. We introduce a pipeline for synthesising realistic crop images for data augmentation. We augment the representation of rare classes using synthetic samples in a ResNet50-based classification pipeline. A strong experimental setup, relying on controlled baselines and synthetic-aided training scenarios, was employed. Evaluation on a real dataset shows significant improvement for the rare class ToLCNDV, with recall increasing from 0.42 in the baseline to 0.81 after synthetic augmentation. In contrast, the performance on other common disease classes remains stable. SSIM, Inception Score, and FID metrics were shown to validate generative quality. At the same time, an ablation study identified a suitable augmentation threshold at which sufficient performance is achieved without excessive synthetic data generation. The results further indicate improvements in feature diversity, which translate into earlier disease recognition (before full disease onset) and improved classification robustness with RareSimGAN. The post-framework combines generative modelling and latent space exploration to deliver a low-cost, scalable, and data-efficient solution for agricultural AI systems. RareAgriDetectAI utility can assist in the proactive monitoring of crop health and simulate rare disease scenarios to drive learning that can aid reliable, interpretable deep learning applications in precision agriculture.
Why it matches plant phenotyping methods希少作物病害の画像合成、病徴進行の可視化、早期病害認識を中心とする画像ベースの植物病害フェノタイピング手法であり、生成品質と分類性能も検証している。
abstractwe propose RareAgriDetectAI, which comprises RareSimGAN, a generative deep learning framework for synthesising images of rare diseases, and a latent traversal mechanism that collaboratively visualises disease progression with increasing severity.
Reproduction assets foundThe paper's data availability statement lists the public plant-disease image datasets used (PlantVillage, AI Challenger mirror, PlantDoc, Tomato Leaf Disease), and the code availability statement provides an authors' GitHub repository containing the RareSimGAN implementation, preprocessing, classifier training, andGradDataset · publicThe datasets analysed during the current study are publicly available from the following sources:
•PlantVillage dataset:
https://www.kaggle.com/datasets/emmarex/plantdiseaseOpen asset ↗pdf-page:36 lines:1-66Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Ustilago maydis is a biotrophic fungus that causes smut disease in maize, leading to tumor formation on aerial parts of the plant. While U. maydis has been a model for plant-fungal interaction studies, no tool has existed to automatically quantify infection symptoms under laboratory conditions for deep learning analysis. To address this, we developed a rotating camera system that captures videos of plants under customized lighting and shutter settings. These videos were used to train machine learning models to distinguish between healthy and infected plants. Two detection approaches have been presented. In the first approach, by employing a naive masking technique and combining classical machine learning classifiers utilizing handcrafted features, the model achieved a reasonable performance, with an Area Under the Curve (AUC) of maximum 0.90 on the Receiver Operating Characteristic in one of the classifiers, showing relatively high sensitivity and specificity. The second approach utilizes pre-trained YOLO11 model for object detection and further classification. The YOLO11-based approach outperforms traditional methods, achieving near-perfect validation accuracy (AUC: 0.99-1.00), demonstrating its superiority for real-time, scalable applications. Our toolset, featuring a cost-efficient and customizable scanning platform with open building-blocks design, provides a valuable resource as a proof-of-concept for unbiased disease symptom detection and scoring, with potential applications in other plant pathology studies. This point enables easy replication and adaptation by other research laboratories which makes the platform robust, scalable and practical beyond our specific application.
Why it matches plant phenotyping methodsトウモロコシの感染症状を画像から自動検出・スコア化する低コスト撮像プラットフォームと機械学習手法の開発が中心であり、植物病害表現型の取得・抽出方法に該当する。
abstractwe developed a rotating camera system that captures videos of plants under customized lighting and shutter settings.
Agriculture is significant in world food production and global economic stability, but leaf disease and pest infection can cause a threat to crop quantity and quality. Thus, it became crucial to have timely and accurate identification of plant leaf disease to prevent loss in agriculture on a large scale and to have sustainable crop management. This paper introduces LeafLiteX, a lightweight mobile-based deep learning application for real-time detection and classification of leaf diseases. This application uses U-Net segmentation to precisely find leaf regions and MobileNetV3-Large to quickly classify diseases with less computation on the computer. The application performs end-to-end processing, from image acquisition to segmentation and disease prediction on mobile devices. An experiment was performed on publicly available crop disease datasets containing various leaf images having different disease types. The model obtained an accuracy of 98.85% showing improved generalization with minimal latency. The design of the model was such that it was suitable for inference on-device while still being robust enough despite changes in lighting conditions, background noise, and camera resolution. LeafLiteX is a low-cost, easy to use, offline-capable, and in-the-moment decision-making supportive diagnostic application that supports farmers and agrarians who require early detection. This paper demonstrates the capabilities that can be achieved using edge-optimized machine learning and computer vision to support the development of smart agriculture technologies. While traditional methods rely solely on classification, this research focuses more on practical implementation by incorporating segmentation, lightweight classification, and explainability to develop a mobile-friendly model.
Why it matches plant phenotyping methods葉画像から病害状態をセグメンテーション・分類する手法とモバイルアプリ自体が研究の中心であり、植物病害の表現型推定に該当する。
abstractThis paper introduces LeafLiteX, a lightweight mobile-based deep learning application for real-time detection and classification of leaf diseases.
Reproduction assets foundThe paper's Data availability statement explicitly links the public PlantVillage (Mendeley) and PlantDoc (GitHub) leaf-image datasets used for its experiments, and provides the authors' LeafLiteX source code on GitHub.Dataset · publicThe dataset used in this study is publicly available from the repository:
https://data.mendeley.com/datasets/tywbtsjrjv/1Open asset ↗data.mendeley.com · tywbtsjrjv/1pdf-page:29 lines:1-74Code · publicThe source code is available on the following link: https://github.com/phdpawan/LeafLiteX.Open asset ↗github.com/phdpawan/LeafLiteXpdf-page:29 lines:1-74Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Cotton leaf diseases present a major threat to global cotton production, significantly impacting both yield and fiber quality. Traditional diagnostic methods are labor-intensive, time-consuming, and demand highly skilled professionals, making them inefficient for large-scale agricultural applications. Although earlier deep learning -based approaches have shown promising results in identifying cotton leaf diseases such as Bacterial Blight, Fusarium Wilt, and Curl Virus Disease, their performance is often limited by complex preprocessing requirements and insufficient generalization to real-world field conditions. To address these challenges, this study proposes and optimized transfer learning-based model, CLDP-CNN, designed to enhance feature extraction and classification efficiency using pre-trained deep neural networks. This study demonstrates the development of Cotton Leaf Disease Prediction Convolutional Neural Network (CLDP-CNN) automatically, utilizing Transfer Learning (TL) which operates on meticulously prepared datasets. Two distinct datasets were used to train the model: the first consisted of field images from cotton farms, while the second was sourced from Kaggle. The main goal of this research examines how the model performs on real-world field datasets. The CLDP-CNN model has proven highly accurate by attaining 99.78% detection success rates for cotton leaf diseases when processing primary dataset which surpasses its secondary dataset accuracy rate of 99.62%. Both the primary dataset and secondary dataset resulted in high accuracy values for the VGG16 pre-trained model which achieved 99.56% accuracy on the primary dataset and 98.82% on the secondary dataset. A web-based application enhances the capabilities of the CLDP-CNN model by providing real-time updates on the health status of cotton plants. This technology empowers farmers with valuable information, enabling them to take timely protective actions to prevent potential severe yield losses in their cotton crops.
Why it matches plant phenotyping methods綿花葉の画像から病害状態を推定する深層学習モデルを開発・評価しており、植物フェノタイピング手法が研究の中心です。
abstractThe main goal of this research examines how the model performs on real-world field datasets.
Reproduction assets foundThe paper's cotton leaf disease image datasets are publicly available: the authors' primary field-collected dataset on the first author's GitHub repository, and the secondary Kaggle dataset used for comparison. No analysis code or trained model checkpoints are explicitly deposited.Dataset · publicbia.
Funding: This work was supported by Princess Nourah bint Abdulrahman
University Researchers Supporting Project number (PNURSP2026R760),
Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Data and code availability
The data that support the findings of this study are openly available in Github and
Kaggle at. https://github.com/mnaeem303/Cotton-Leaf_Disease-Dataset),
and https://www.kaggle.com/datasets/seroshkarim/cotton-leaf-disease-dataset/data
Author Contributions
All the authors (Muhammad Naeem, Muhammad Ibrahim, Nadeem Sarwar, Oumaima
Saidani, Asma Irshad, Muhammad Shadab Alam Hashmi, Muhammad Tayyab
Qammar) contributed equally to this work in their respective meaningOpen asset ↗https://github.com/mnaeem303/Cotton-Leaf_Disease-Datasetpdf-raw-page:29 lines:1-54Dataset · publicbdulrahman
University Researchers Supporting Project number (PNURSP2026R760),
Princess Nourah bint Abdulrahman University, Riyadh, Saudi Arabia.
Data and code availability
The data that support the findings of this study are openly available in Github and
Kaggle at. https://github.com/mnaeem303/Cotton-Leaf_Disease-Dataset),
and https://www.kaggle.com/datasets/seroshkarim/cotton-leaf-disease-dataset/data
Author Contributions
All the authors (Muhammad Naeem, Muhammad Ibrahim, Nadeem Sarwar, Oumaima
Saidani, Asma Irshad, Muhammad Shadab Alam Hashmi, Muhammad Tayyab
Qammar) contributed equally to this work in their respective meaningful ways. All the
authors have read and approved the final manuOpen asset ↗pdf-raw-page:29 lines:1-54Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Food productivity, quantity and quality are at stake when plant diseases such as rice diseases undermine the food security. Rice leaf disease treatment necessitates accurate and timely diagnosing. This study describes a deep learning model for categorizing and forecasting rice plant diseases. Using the remora optimization algorithm (ROA) on a rice leaf dataset demonstrates its potential for plant disease classification. The ROA-DM method detects rice leaf diseases using the ROA algorithm, a deep maxout network (DMN), and a deep autoencoder (DAE). ROA is applied to the learning parameters of deep model in order to achieve better convergence and avoiding local minima, which usually happens with conventional gradient-based optimizers. Experiments show that the suggested framework is accurate and precise across illness categories. The confusion matrices display the training and validation accuracy, losses of this model. The performance of our optimal learning method with respect to other methods indicated its potential for identifying leaf diseases. The accuracy of the ROA-DM method is 98.5%.
Why it matches plant phenotyping methodsイネ葉の観察画像から病害状態を分類・検出する深層学習手法が研究の中心であり、植物病害フェノタイピング手法に該当する。
abstractThis study describes a deep learning model for categorizing and forecasting rice plant diseases.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe dataset collected from https://www.kaggle.com/datasets/emmarex/plantdisease (PlantVillage dataset) for algorithm testing in plant disease diagnosis 35 . The selected rice leaf disease samples from PlantVillage dataset consisting of 3050 colour leaf images across four classes.Open asset ↗Kaggle · emmarex/plantdiseaselines:85-97Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Spatio-Temporal Fusion (STF) has been widely used across various remote sensing applications, including environmental monitoring, land cover change detection, and water resource management by integrating multi-sensor data with different spatial and temporal resolutions. The objective of this study was to generate spatially and temporally fine-resolution imagery and to evaluate the performance of multiple STF algorithms and Consistent Adjustment of the Climatology to Actual Observations (CACAO) post-processing for parcel-level crop monitoring. The study was conducted in a soybean field located in Anseong, South Korea, covering the entire soybean growing season from late June to early November. Near-daily Planet SuperDove imagery with 3 m resolution was used to temporally enhance UAV images, which were acquired at 0.05 m resolution but only at weekly to monthly intervals. Through the downscaling process, the UAV data were converted into a daily dataset with a target spatial resolution of 0.5 m. Relative radiometric normalization was applied, followed by the implementation and comparison of four STF algorithms- Enhanced Spatial and Temporal Adaptive Reflectance Fusion Model (ESTARFM), Fitting, spatial Filtering and residual Compensation (Fit-FC), Flexible Spatiotemporal Data Fusion (FSDAF), and Variation-based Spatiotemporal Data Fusion (VSDF)-within a 4-fold cross-validation framework. CACAO post-processing was then employed to reconstruct temporally continuous Normalized Difference Vegetation Index (NDVI) and Enhanced Vegetation Index (EVI) trajectories, from which the NDVI-based Vegetation Growth Metrics (VGM)85 and the EVI-based VGMmax were derived. The validation results indicated that ESTARFM achieved the highest NDVI performance among the evaluated algorithms, with a Root Mean Square Error (RMSE) of 0.113 and the Universal Image Quality Index (UIQI) of 0.697. CACAO post-processing further improved these results, with CA-ESTARFM achieving an RMSE of 0.108 and a UIQI of 0.740, corresponding to a 4.4% reduction in RMSE and a 6.2% improvement in UIQI relative to the baseline ESTARFM. NDVI histogram and spatial analyses demonstrated that CA-ESTARFM achieved the most consistent agreement with UAV observations while preserving fine-scale spatial heterogeneity. In addition, intra-field vegetation assessment using NDVI-based VGM85 and EVI-based VGMmax showed that CA-ESTARFM remained consistent with simple linear interpolation of UAV observations while retaining finer spatial structure and reducing localized noise in the derived growth metrics. The proposed framework demonstrates strong potential for applications in comprehensive crop monitoring, precision agriculture management, and yield forecasting.
Why it matches plant phenotyping methodsUAV・衛星画像の時空間融合とCACAO処理により、NDVI/EVIおよび植生成長指標を抽出するワークフローを開発・比較検証しており、植物状態の取得手法が中心である。
abstractThe objective of this study was to generate spatially and temporally fine-resolution imagery and to evaluate the performance of multiple STF algorithms and Consistent Adjustment of the Climatology to Actual Observations (CACAO) post-processing for parcel-level crop monitoring.
Reproduction assets foundThe paper's STF/CACAO analysis code is openly available on Zenodo. The underlying Planet/UAV imagery data are only available from the corresponding author upon request, so they qualify as request_only.Code · publicThe code supporting this study is openly available at Zenodo
(https://doi.org/10.5281/zenodo.20923823).Open asset ↗Zenodo · 10.5281/zenodo.20923823pdf-page:20 lines:1-70Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Paddy leaf disease (PLD) detection has grown more difficult, yet early detection might prevent significant losses due to decreased crop yield. However, existing models struggle to accurately classify diseases under difficult circumstances like intricate backgrounds, fluctuating lighting, and overlapping leaves. Additionally, existing models do not incorporate efficient optimization strategies, leading to suboptimal accuracy and poor generalization on unseen data. To address these challenges, a novel deep learning-based YOLO-LEAFNET method for PLD detection utilizing IGT-YOLO, integrating the YOLOv8 disease detection with the Improved Gorilla Troops (IGT) optimization. The input paddy leaf images are pre-processed using Bilateral Contrast Limited Adaptive Histogram Equalization (B-CLAHE) to enhance image quality and improve local contrast while preserving disease boundaries. YOLOv8 model is utilized to detect and classify paddy leaf diseases by accurately localizing affected regions with bounding boxes. Then, the IGT algorithm boosts the disease detection accuracy by optimizing YOLOv8 through effective hyperparameter tuning. The proposed YOLO-LEAFNET method effectiveness was evaluated using recall, F1 score, specificity, accuracy, and precision. B-CLAHE enhanced noise-free images improve contrast and detection accuracy, while the IGT-YOLO model ensures scalable, efficient early diagnosis of paddy leaf diseases with 99.07% accuracy. The YOLO-LEAFNET enhanced the total accuracy by 3.21%, 5.25%, and 1.98% related to CNN, DeepRice, and FasterR-CNN, respectively.
Why it matches plant phenotyping methodsイネ葉の病害状態を画像から検出・分類するYOLOベース手法を提案し、前処理・最適化・性能評価を中心に扱っているため、植物フェノタイピング手法として該当する。
abstractTo address these challenges, a novel deep learning-based YOLO-LEAFNET method for PLD detection utilizing IGT-YOLO, integrating the YOLOv8 disease detection with the Improved Gorilla Troops (IGT) optimization.
Reproduction assets foundThe paper's phenotyping input is the public UCI Rice Leaf Diseases dataset (paddy leaf images of bacterial leaf blight, leaf smut, brown spot), also mirrored on Kaggle. No author analysis code, trained model, or supplementary assets are disclosed.Dataset · publicThe dataset is publicly available
at: https://archive.ics.uci.edu/dataset/486/rice+leaf+diseases. The dataset is distributed
under the Creative Commons Attribution 4.0 (CC BY 4.0) license.Open asset ↗rice+leaf+diseasespdf-page:7 lines:1-33Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
This paper presents a deep learning model to detect cotton plant pests and classify diseases, which must overcome limited datasets, class imbalance, and feature redundancy. At the preprocessing phase, the Gaussian blur filtering and Contrast Limited Adaptive Histogram Equalization (CLAHE) are used to sharpen images by improving their clarity and contrast. To increase and diversify the data, Spa-GAN-based data augmentation is used to produce realistic synthetic samples. To obtain an accurate Region of Interest (RoI), an Attention-Guided Multi-Scale Residual U-Net (AGMS-U-Net) is considered to segment local and global structural information. The proposed framework makes three major contributions: (i) a new attention-based feature extractor, TriAttnNet, that incorporates spatial, channel, and contextual attention to represent diseases on a fine-grained level (ii) a new optimization strategy, Hybrid Mongoose Ray Chaotic Optimization (HMRCO), which includes chaotic strategies to better tune the parameters and explore the feature space and (iii) classification layer with focal loss for final decision. Experimental analyses prove that the suggested method is much more effective than the current state-of-the-art models, providing a powerful and understandable solution to precision agriculture and sustainable cotton crop health monitoring. Experimental results show that TriAttnNet achieves 98.66% accuracy, 98.71% recall, and 98.81% F1-score, which is better than the state-of-the-art algorithms, such as EfficientNetB1-CBAM (96.38%) and BERT-ResNet-PSO (95.69%). The proposed system is computationally feasible and interpretable, and it is interpretable to provide a practical solution to precision agriculture and sustainable monitoring of the health of cotton crops.
Why it matches plant phenotyping methods綿花植物の画像から病害を分類する深層学習手法を開発・評価しており、植物の病害状態を直接推定する方法が研究の中心である。
abstractThis paper presents a deep learning model to detect cotton plant pests and classify diseases
Reproduction assets foundThe paper's plant-phenotyping input is the public Kaggle Cotton Plant Disease Dataset (Dhamodharan R), explicitly cited as the study's data source with a matching public URL. The authors' model/code is not publicly deposited (available only upon request), so no qualifying code asset exists.Dataset · publicThe dataset of this study is taken from the publicly available Kaggle repository, Cotton Plant Disease Dataset 43Open asset ↗Kagglelines:48-58Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Tomatoes are the most significant and widely consumed crops globally. Leaf diseases cause an important threat to crop production and quality. Further, various fungi, bacteria and viruses can influence the plant's various parts and gradually destroy the quality and production of tomatoes in an agricultural field, thus it impacts the surrounding cultivated plants to cause more economical loss to farmers. Therefore, various techniques are proposed recently to optimally recognize and categorize the epidemic pathogens. To enhance sustainable plant protection practices, accurate identification and classification of diseases is essential to enhance the production rates. Effective pathogen detection and monitoring of plant health are critical areas of research in agriculture. Understanding disease severity is a crucial role for management practices and preventing the spread of infections. Rapid assessment is crucial because early detection of disease can significantly enhance the crop yield and influence the management strategies implemented by farmers. In this proposed model, a deep learning approach is proposed to classify the severity of diseases in tomato plants. At first, the needed images are collected from publicly available resource. Further, the collected images are subjected to the Adaptive and Attention-based Mask Region Convolutional Neural Network (AA-MRCNN) for optimally segmenting the abnormal regions from the gathered image. Further, the hyperparameters, like epoch, steps per epoch, and hidden neuron count in the Adaptive and Attention-based Mask Region Convolutional Neural Network are tuned by the Fitness-based African Vultures Optimization (FAVO) algorithm. Also, the segmented images are passed into Multiscale Recurrent MobileNet (MRMNet) module for categorizing disease severity in the tomato plant epidemic. The assessment of the recommended severity detection approach of tomato plant disease is determined by conducting a simulation experiment. The proposed model attains better outcomes of 93% accuracy, 93% specificity, 93% precision, 7% False Negative Rate (FNR), 86% Matthews Correlation Coefficient (MCC), 93% Fowlkes mallow Index (FM), 86% Bookmaker Informedness (BM), and 86% Threat Score (TS) measures in the ReLu activation function, which is progressed than the conventional frameworks. The result defines that the suggested technique outperformed than other baseline models to ensure the dependability of the tomato plant epidemic pathogens detection performance.
Why it matches plant phenotyping methodsトマト葉画像から病変領域を分割し、植物病害の重症度を分類する画像ベースの表現型推定手法を開発・評価しており、方法が研究の中心である。
abstracta deep learning approach is proposed to classify the severity of diseases in tomato plants.
Reproduction assets foundThe paper uses a public Kaggle tomato leaf disease image dataset as its phenotyping input and states that the authors' source code is available in a public GitHub repository. Both are paper-specific, publicly accessible, and actionable.Code · publiche tomato disease classification performances were
carried out among the performance metrics like Prevalence Threshold
(PT), BM, Precision, FNR, Accuracy, FM, Specificity, MK (Markedness)
and TS to maximize the reliability of the designed approach. The source
code of the public repository on GitHub link is available on “GitHub-
https://github.com/pdeepika6078/Severity-Classification-of-Tomato-Plant-Epidemic-Pathogens-/tree/main”
In order to demonstrating the effectiveness of the designed approach,
several conventional segmentation, optimization, and classification
approaches are adopted to compare the overall process. The reason
behind selecting the traditional approaches to improve the clasOpen asset ↗pdeepika6078/Severity-Classification-of-Tomato-Plant-pdf-raw-page:55 lines:1-29Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Global food security is largely based on the accurate and timely diagnosis of crop diseases, where paddy rice is an extremely essential staple of more than half of the world population. The conventional disease identification techniques tend to be laborious, time consuming and demand a great deal of domain knowledge, which becomes a bottleneck in the efficient management of the farms. Although deep learning [and especially Convolutional Neural Networks (CNNs)] have demonstrated a spectacular performance in automated classification of diseases based on leaf images, they tend to overlook important contextual features that are implicitly processed by agronomic experts. The visual defects of a disease might be unclear and this can greatly differ depending on factors like the genetic variety of the plant and the stage of development. We overcome this shortcoming by proposing a new multi-modal deep learning framework, Multi-Modal Factorized Bilinear Pooling (MFBP) model which is capable of a more holistic and precise paddy health measurement. The proposed method is the only one that combines high-level visual information obtained using leaf images and related tabular information, namely the paddy type and number of days. The MFBP model uses Factorized Bilinear Pooling (FBP) rather than the simple feature concatenation which commonly loses the complex relationship between different data types. This systematic method efficiently encodes all the complex interactions between all components of the visual and tabular features vectors in such a way that helps the model to pick up subtle, context-specific patterns. As an example, it will only be possible to educate the model that a specific visual blemish is predictive of a given disease through a specific species at a specific age. We test our model on the Paddy Doctor: Paddy Disease Classification dataset, which is a detailed public dataset comprising of more than 10,000 labeled images and containing relevant metadata, and thus it forms a perfect testing bed to conduct multi-modal research. Through our detailed experiments, we have shown that the proposed MFBP model is much better than a baseline model based on concatenation fusion, which proves that deep, multiplicative interactions can be best modeled in this task. The findings highlight the massive possibilities of multi-modes AI in the development of more robust, more accurate, and more context-aware diagnostic instruments and precision agriculture to enable more sustainable and productive agricultural activities.
Why it matches plant phenotyping methods葉画像とメタデータを統合してイネの健康状態・病害を推定する新規深層学習手法を提案し、ベースライン比較で検証しているため、植物フェノタイピング手法が中心である。
abstractWe overcome this shortcoming by proposing a new multi-modal deep learning framework, Multi-Modal Factorized Bilinear Pooling (MFBP) model which is capable of a more holistic and precise paddy health measurement.
Reproduction assets foundThe paper's phenotyping inputs are the public Kaggle 'Paddy Doctor: Paddy Disease Classification' dataset (10,407 leaf images with tabular metadata for variety and age), explicitly named in the Data Availability statement with a persistent public URL. No author analysis code, trained models, or checkpoints are reportedDataset · publicThe datasets used and/or analysed during the current study are publicly available in the
“Paddy-doctor: paddy disease classification” repository at the following persistent
URL: https://www.kaggle.com/datasets/vbookshelf/paddy-disease-classification.Open asset ↗Kaggle · vbookshelf/paddy-disease-classificationpdf-page:20 lines:1-74Code / dataset availability confirmedCrossref · Europe PMC · checked 15 Sept 2026
Image processing plays a vital role in precision agriculture by enabling automated disease detection and crop health monitoring. This research presents a novel Hybrid Vision Transformer with Dense Attention Capsule Network (HVT-DACapNet) model for accurate cotton plant disease detection. The proposed framework integrates Adaptive Wavelet Transform Filtering (AWTF) for noise removal while preserving disease-related features. A Hybrid Vision Transformer (HVT) is employed to extract both local spatial patterns and global contextual dependencies, and the Dense Attention Capsule Network (DACapNet) captures hierarchical spatial relationships with an attention mechanism that emphasizes infected regions. In addition, a hybrid optimization strategy combining Mayfly and Aquila Optimization (HMAO) is used to fine-tune model hyperparameters for improved convergence. The model is evaluated on a publicly available Kaggle cotton leaf disease dataset containing healthy leaves and multiple disease categories including Target Spot, Powdery Mildew, Bacterial Blight, Army Worm, and Aphids, using a 70:15:15 train-validation-test split under the simulation setup and hyperparameter configuration described in the manuscript. The proposed HVT-DACapNet achieves an F1-score of 99.68%, sensitivity of 99.68%, specificity of 98.89%, and an overall accuracy of 99.79%, outperforming existing models such as ConvLSTM-ZOA, GOA, SFO, Inception-V3, and VGG-16.
Why it matches plant phenotyping methods綿花葉の画像から病害状態を推定する深層学習手法を新規開発し、公開データセットで性能評価しているため、植物フェノタイピング手法が中心である。
abstractThis research presents a novel Hybrid Vision Transformer with Dense Attention Capsule Network (HVT-DACapNet) model for accurate cotton plant disease detection.
Reproduction assets foundThe paper's only qualifying asset is the public Kaggle Cotton Plant Disease Dataset used as the input image dataset for all experiments. The authors' code is explicitly not publicly available (institutional restrictions), with only a supplementary algorithm document and on-request implementation details.Dataset · publicthodological workflow of the proposed model. Additional
implementation details may be made available from the corresponding
author upon reasonable request for academic and non-commercial
research purposes.
Data Availability-The datasets generated and/or analysed during the
current study are available in the Kaggle repository,
https://www.kaggle.com/datasets/dhamur/cotton-plant-disease
Author’s contribution – G.Neelavathi– Research proposal –
construction of the workflow and model – Final Drafting– Survey of
Existing works – Improvisation of the proposed model;
Dr.K.Venkatasalam – Initial Drafting of the paper – Collection of
datasets and choice of their suitability – Formulation of pseudOpen asset ↗Kaggle · dhamur/cotton-plant-diseasepdf-raw-page:39 lines:1-42Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Pearl millet is an important crop in arid regions, but its yield is reduced by foliar diseases like Downy Mildew and Rust. Traditional and deep learning methods struggle with accurate lesion detection, severity estimation, and robustness under complex field conditions, and often lack interpretability for practical agricultural deployment. To address these challenges, this study proposes the Adaptive Severity-Aware Swin Attention Network (ASA-SAN), an integrated framework designed for disease segmentation, classification, and severity estimation in pearl millet leaves. The proposed architecture combines a Swin Transformer encoder for hierarchical feature extraction with a ResUNet++ decoder for accurate lesion segmentation. This is further enhanced using Adaptive Channel Attention to improve feature discrimination and a dual-stream classification network to jointly capture local lesion characteristics and global contextual information. Additionally, an Adaptive Disease Severity Index (ADSI) is introduced to quantitatively assess disease progression based on lesion area ratio, color degradation, edge irregularity, and texture variations. Experimental evaluations conducted on a pearl millet leaf dataset demonstrate that the proposed method achieves a Dice score of 97.8%, IoU of 95.6%, classification accuracy of 98.3%, and F1-score of 98.2%, outperforming several state-of-the-art methods. Furthermore, Grad-CAM visualizations enhance model interpretability by highlighting disease-relevant regions. Overall, the ASA-SAN framework provides a robust, interpretable, and severity-aware solution for automated pearl millet disease analysis, enabling early detection and supporting precision agriculture practices for improved crop protection and yield optimization.
Why it matches plant phenotyping methods真珠粟葉の病斑を画像から分割・分類し、病害重症度を定量推定する手法を中心に開発・評価しているため、植物表現型計測手法として含める。
abstractAdditionally, an Adaptive Disease Severity Index (ADSI) is introduced to quantitatively assess disease progression based on lesion area ratio, color degradation, edge irregularity, and texture variations.
Reproduction assets foundThe paper's phenotyping inputs are drawn from a public, open-access image dataset: the Pearl Millet Leaf Disease dataset (Version 2) hosted on Roboflow Universe, containing annotated images of Downy Mildew, Rust, and healthy pearl millet leaves. This is a paper-specific, publicly available asset directly used for the作者Dataset · publicThe dataset used in this research was taken from the publicly available open-access Pearl
Millet Leaf Disease dataset hosted on Roboflow Universe, which has images of Downy
Mildew, Rust and healthy pearl millet leaves annotated publicly available [26]. To ensure
experimental consistency and reproducibility, all experiments were conducted with Version
2 of the open access dataset.Open asset ↗Roboflow Universepdf-raw-page:10 lines:1-28Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Plant diseases cause 20-40% annual crop losses worldwide, yet conventional detection methods remain slow, subjective, and inaccessible to smallholder farmers. This work presents GreenAid, an end-to-end plant disease detection and management system that bridges the gap between laboratory-level deep learning performance and practical agricultural deployment. The system integrates a confidence-weighted ensemble of three CNN architectures (VGG16, ResNet50, InceptionV3), fused through per-class F1-score reliability weights, with a cross-platform mobile application supporting offline inference via TensorFlow Lite, a web-based analytics dashboard, and an NLP-powered chatbot. On the PlantVillage benchmark (87,000 images, 38 classes, 14 species), the ensemble achieves 98.74% accuracy and 98.48% F1-score. Systematic comparison of six fusion strategies confirms that per-class F1 weighting outperforms alternatives including majority voting, simple averaging, and stacking. The INT8-quantised deployment model (78 MB, 127 ms on a mid-range smartphone) retains 98.43% accuracy with per-class analysis confirming disproportionate impact on the five most challenging categories. All pairwise model comparisons are validated by McNemar's test ([Formula: see text]). The primary contribution is the complete, reproducible integration of competitive classification, edge deployment, and an end-to-end agricultural delivery pipeline (mobile application, web dashboard, and NLP chatbot) rather than the ensemble mechanism itself.
Why it matches plant phenotyping methods植物画像から病害状態を推定する深層学習手法の開発・比較検証と、モバイル実装が中心であり、植物病害フェノタイピング手法として適格。
abstractThis work presents GreenAid, an end-to-end plant disease detection and management system
Reproduction assets foundThe paper's plant-disease phenotyping analysis is built on the public PlantVillage dataset (87,000 leaf images, 38 classes), which the authors explicitly state is publicly accessible via Kaggle. No authors' analysis code, trained models, or checkpoints are released with an explicit public URL in the supplied blocks.Dataset · publicThe dataset used in this study is the publicly available PlantVillage dataset, accessible via Kaggle at:Open asset ↗Kagglelines:270-340Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Wheat (Triticum aestivum L.) is a staple crop of paramount importance to global food security; however, its productivity is significantly compromised by foliar diseases. Conventional diagnostic approaches, relying on manual observation or laboratory analyses, are often labor-intensive and susceptible to inaccuracies. While recent advancements in deep learning present promising avenues for automated disease detection, persistent challenges such as limited annotated datasets, environmental heterogeneity, and model generalization continue to hinder optimal performance. This study proposes a novel hybrid deep learning model called HybridViT, which combines ConvNeXt and Vision Transformer (ViT) architectures with the Convolutional Block Attention Module (CBAM) to improve the classification of wheat leaf diseases. While ConvNeXt ensures local feature extraction and ViT provides global contextual understanding, CBAM dynamically highlights the most discriminative features. Additionally, the Contrast Limited Adaptive Histogram Equalization (CLAHE) method is employed to enhance the visibility of disease symptoms in low-contrast leaf images. Unlike conventional hybrid CNN-Transformer approaches that rely on static feature concatenation, the proposed model employs an adaptive gated fusion mechanism to dynamically balance local and global feature representations. The fused features are further refined using a lightweight CBAM module to enhance discriminative capability. Additionally, Contrast Limited Adaptive Histogram Equalization (CLAHE) is applied to improve feature visibility under varying illumination conditions. Evaluated on three different datasets obtained under both controlled and field conditions, HybridViT achieved 100% accuracy on balanced datasets and 99.10% accuracy on complex images captured in real-world conditions, surpassing existing methods. Furthermore, a 5-fold cross-validation strategy yielded an average accuracy of 99.04% ± 0.22, demonstrating the model's robustness and stability across different data splits. The results demonstrate the model's robustness against environmental noise, lighting variations, and class imbalance. This approach, which enables early and accurate disease diagnosis, supports sustainable agricultural practices, reduces pesticide use, and contributes to global food security.
Why it matches plant phenotyping methods小麦葉の病徴を画像から分類する深層学習手法を開発・検証しており、植物病害状態の取得・推定が研究の中心です。
abstractThis study proposes a novel hybrid deep learning model called HybridViT, which combines ConvNeXt and Vision Transformer (ViT) architectures with the Convolutional Block Attention Module (CBAM) to improve the classification of wheat leaf diseases.
Reproduction assets foundThe paper evaluates HybridViT on three public wheat leaf disease image datasets from Kaggle, cited in the reference list with explicit URLs. These are the paper-specific image inputs used for its disease-classification measurements. No author analysis code, trained model checkpoints, or supplementary code deposit is披露dDataset · publicAvailable: https://www.kaggle.com/datasets/olyadgetch/wheat-leaf-datasetOpen asset ↗Kaggle · olyadgetch/wheat-leaf-datasetpdf-page:51 lines:1-64Dataset · public[78] J. Jayaprakash, “Wheat Leaf Disease,” Kaggle. Accessed: May 1, 2026.
[Online]. Available:
https://www.kaggle.com/datasets/jayaprakashpondy/wheat-leaf-diseaseOpen asset ↗Kaggle · jayaprakashpondy/wheat-leaf-diseasepdf-page:51 lines:1-64Dataset · public[79] S. Kumar, “Multiple Plant Diseases Dataset,” Kaggle. Accessed: May 1, 2026.
[Online]. Available:
https://www.kaggle.com/datasets/samareshkumar/multipleplantdiseasesOpen asset ↗Kaggle · samareshkumar/multipleplantdiseasespdf-page:51 lines:1-64Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The global food security is severely threatened by various bacterial and fungal diseases that significantly degrade the quality, yield and productivity of wheat crop. This increases the need for an accurate and efficient system to improve wheat yield and mitigate these losses by enabling early intervention. The dataset used in this research comprises of 10,000 images from brown rust, yellow rust, powdery mildew, loose smut diseases and healthy wheat plants. The existing neural networks, ensembling and transformer-based models used for classifying wheat diseases are limited by high computational resource requirements that leads to inefficient feature extraction. These challenges are addressed by proposing a customized, lightweight and optimized Swin-Streamlined High Accuracy and Reduced Parameters (Swin-SHARP) transformer, a lightweight and optimized transformer model that enhances feature extraction while significantly reducing computational overhead. In particular, Swin-SHARP results in 82.5% reduction (48.9M to 8.5M parameters), making it an attractive solution for resource-constrained environments. The extracted features are further optimized by integrating the Swin-SHARP transformer with boosted Model-Agnostic Meta-Learning (MAML) and a weighted ensembling strategy to enhance generalization and classification accuracy. Our proposed model achieves a remarkable 98.1% accuracy, significantly outperforming existing CNN-based solutions, ensemble approaches, transformer, and deep learning models. We also cross-validated our proposed model on an unseen wheat plant diseases dataset, achieving 95.57% accuracy. Our proposed model is also compared against prominent models such as Inception-v3, ResNet-18, and VGG-16, which outperforms them by 1.6%, 1.7%, and 2.6%, respectively. The comparison with existing state-of-the-art models, including Sequential CNN, SGDR-S, Inception-v3, Cereal Conv, Darknet-53 CNN, EfficientNet B3, GLNet, CNN & SVM, Customized CNN, CaiT-YOLOv9 and MSFNet revealed that our method outperforms them by 0.6%, 5.8%, 5.3%, 0.75%, 2.8%, 2.68%, 1.42%, 1.3%, 3.31%, 3.29%, and 2.4% respectively. These results demonstrate the effectiveness and practicality of the Swin-SHARP transformer for wheat disease classification, particularly for real-time agricultural applications on mobile and embedded systems aimed at early disease detection and crop management.
Why it matches plant phenotyping methods小麦の病徴画像から植物の病害状態を推定する深層学習手法を開発し、別データセットで交差検証しており、植物表現型取得・判定が研究の中心である。
abstractproposing a customized, lightweight and optimized Swin-Streamlined High Accuracy and Reduced Parameters (Swin-SHARP) transformer
Reproduction assets foundThe paper's Data Availability Statement explicitly states that the authors' wheat disease dataset and analysis code are publicly available on GitHub (https://github.com/SWIN-SHARP/), which is a paper-specific, actionable asset. The paper also uses third-party public datasets (Zindi ICLR Workshop, Mundi, Watershed/GrabcCode · publicThe dataset and code used in this research have made publicly available on https://github.com/SWIN-SHARP/
SWIN-SHARP for reproducibility purposes.Open asset ↗SWIN-SHARPpdf-page:25 lines:1-104Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Accurate and efficient identification of cucumber leaf diseases is a critical step in preventing losses and facilitating timely intervention in agricultural activi-ties. However, most state-of-the-art plant disease recognition models, including those employing deep learning, often fail to identify spatial dependencies among symptomatic leaf feature regions, require high computational resources, and lack robustness in their predictions. To overcome these challenges, this paper pro-poses MobileGraph, a graph-aided deep learning model that jointly reasons local texture patterns and spatial dependencies among CNN-derived cucumber leaf feature regions using MobileNetV3 as a lightweight feature extractor. Experi-ments on a publicly available cucumber leaf disease dataset containing 5 classes and 4,000 images show that the proposed model achieves 99.75% accuracy, 99.75% macro F1-score, and 99.69% MCC, outperforming several state-of-the-art models including ResNet-152, EfficientNet-B7, DenseNet-201, ConvNeXt, and VGG16, while having a significantly lower computational cost of 0.465 GFLOPs. Explainability results from Grad-CAM and LIME indicate that the model is focused on biologically important regions of plant lesions. Furthermore, a proto-type mobile application illustrates the feasibility of real-time cucumber disease diagnosis for practical agricultural monitoring. These results indicate that Mobi-leGraph provides an efficient and interpretable solution for intelligent crop health surveillance.
Why it matches plant phenotyping methodsキュウリ葉の病斑という植物状態を画像から診断する深層学習手法を開発し、複数モデルとの性能比較・検証まで行っており、病害表現型の取得・推定が研究の中心である。
abstractExplainability results from Grad-CAM and LIME indicate that the model is focused on biologically important regions of plant lesions.
Reproduction assets foundThe paper's experiments use the publicly available Cucumber Disease Recognition Dataset (4,000 images, 5 classes) hosted on Mendeley Data, which is a paper-specific public phenotype/image asset. The MobileGraph source code is only available upon request, so it does not qualify as a public asset.Dataset · publicThe dataset analysed of this study, titled ”Cucumber Disease Recognition
Dataset” is publicly available in the Mendeley Data repository at
(https://data.mendeley.com/datasets/y6d3z6f8z9/1).Open asset ↗Mendeley Data · y6d3z6f8z9pdf-page:36 lines:1-71Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Early and accurate disease detection is important for increasing the agricultural output, decreasing the financial costs, and ensuring food security. Traditional diagnostic procedures take much time and effort, involve the necessity of having deep expertise, and are not always suitable for large scale farming disease detection. For this purpose, the current research suggests developing an explainable lightweight CNN-based model for crop disease identification based on RGB leaf images. The model utilizes several innovative architectural solutions such as depth-wise separable convolution, SE blocks, skip connections, and guided attention-based feature learning that allow enhancing the effectiveness of features extraction and decreasing computation load. Moreover, Grad-CAM is used to visualize affected areas on a map and thus increase the interpretability of the model. The suggested solution was implemented and tested on the PlantVillage dataset containing 54,305 images for 38 crop diseases out of 14 crops. The results show that the training, validation, and testing accuracies equal 97.6%, 88.3%, and 97.63%, correspondingly, along with the Macro-F1 measure of 0.867 and Micro-ROC-AUC equal to 0.99. A comparative study reveals that the presented model performs comparably well in terms of classification with lightweight structure and built-in interpretability capabilities to be applied in the mobile and edge-enabled agriculture environment. The results show that the presented approach is capable of being used as an effective and interpretable tool for diagnosing plant diseases in real-time.
Why it matches plant phenotyping methods葉画像から植物病害を推定するCNN手法の開発・評価が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として含める。
abstractthe current research suggests developing an explainable lightweight CNN-based model for crop disease identification based on RGB leaf images.
Reproduction assets foundThe paper's plant-phenotyping input is the public PlantVillage leaf-image dataset (54,305 RGB images, 38 crop-disease classes), explicitly declared in the Data availability statement with a Kaggle URL. No author code, trained model, or checkpoint is deposited.Dataset · publicThe data set analyzed during current study are available in https://www.kaggle.com/datasets/emmarex/plantdisease.Open asset ↗Kaggle · emmarex/plantdiseaselines:366-390Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
In-season estimation of wheat grain yield potential is critical for crop management and advancing breeding efforts. Spike and spikelet counts serve as key indicators directly linked to yield potential, yet their assessment still relies on manual counting which is both labor-intensive and error-prone. High-resolution digital (RGB) imagery combined with deep learning-based object detection methods has substantially advanced automatic wheat spike detection and counting. However, precise spikelet-level phenotyping remains largely underexplored. This study evaluates two recent YOLO variants, YOLOv11 and YOLOv12, for wheat spike and spikelet detection and counting using oriented bounding boxes (OBB), and introduces a new large-scale benchmark dataset comprising 48,521 spike and 60,404 spikelet instances with OBB annotations. For spike detection, the pre-trained YOLOv11 achieved superior accuracy (mAP@0.5 = 95.8%, Pearson r = 0.993) with shorter training and inference times compared to YOLOv12. For spikelet detection, the non-pretrained YOLOv11 demonstrated higher accuracy (mAP@0.5 = 99.0%), while counting performance was comparable across models. These results establish OBB-based YOLO detection as a robust and scalable approach for AI-driven wheat phenotyping.
Why it matches plant phenotyping methods小麦の穂・小穂という収量関連形質の画像ベース検出・計数手法を比較評価し、大規模ベンチマークデータセットも構築しているため、フェノタイピング手法が中心である。
abstractThis study evaluates two recent YOLO variants, YOLOv11 and YOLOv12, for wheat spike and spikelet detection and counting using oriented bounding boxes (OBB), and introduces a new large-scale benchmark dataset comprising 48,521 spike and 60,404 spikelet instances with OBB annotations.
Reproduction assets foundThe paper openly states its supporting data (spike/spikelet imagery with OBB annotations) is available on Zenodo, and the underlying models are deployed on the authors' public WheatAI cloud platform.Dataset · publicData availability
The data supporting the findings of this study are openly available at: https://doi.org/10.5281/zenodo.20215489 .Open asset ↗zenodo · 10.5281/zenodo.20215489lines:219-266Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Early and accurate detection of apple leaf diseases is critical for sustainable agriculture, yet manual diagnosis remains time-consuming and error-prone. This study introduces a novel deep learning framework centered on a custom ContinuousLayer, a spatially adaptive convolutional layer designed to overcome the limitations of standard CNNs. This architecture automates the classification of apple leaf diseases Black rot, rust, scab, and healthy leaves with high precision. The model addresses dataset imbalance through strategic resampling, achieving uniform class distribution. The ContinuousLayer introduces spatial feature modulation using trainable Gaussian basis functions, enhancing feature extraction while penalising kernel irregularities through a hybrid composite loss function. Trained on a dataset of 3,164 images balanced via bicubic up-sampling, and evaluated on a held-out test set of 10% of the data, the model attains a 98.63% test accuracy, with F1-scores ranging from 0.98 to 1.00 across classes. Visual analysis of the confusion matrix reveals minimal misclassification, predominantly between rust and scab. Comparative evaluation against baseline architectures demonstrates the efficacy of the ContinuousLayer in capturing disease-specific spatial patterns. These results underscore the potential of integrating mathematically inspired layers into CNNs for plant pathology applications, offering a highly accurate tool for precision agriculture in controlled environments.
Why it matches plant phenotyping methodsリンゴ葉の病徴を画像から分類する新規深層学習層と解析手法を開発・比較評価しており、植物病害状態の表現型抽出が中心である。
abstractThis study introduces a novel deep learning framework centered on a custom ContinuousLayer, a spatially adaptive convolutional layer designed to overcome the limitations of standard CNNs.
Reproduction assets foundThe paper's apple leaf disease image dataset (3,164 images) is explicitly stated to be publicly available on Kaggle, matching an allowed URL. No author code or model checkpoints are reported as available.Dataset · publicThe datasets analysed during the current study is publicly available in the Kaggle repository at https://www.kaggle.com/datasets/mhantor/apple-leaf-diseases.Open asset ↗Kaggle · mhantor/apple-leaf-diseaseslines:595-613Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Field / plotClassificationDisease symptoms / severity
This research discusses the incorporation of IoT with blockchain technique to enhance the efficiency of smart farming systems, particularly focusing on plant disease classification, pest detection, and smart irrigation. The study aims to develop a secure and effective IoT-based smart farming framework using the Ethereum blockchain to store and transmit data, and a Hybrid Convolution Adaptive Recurrent MobileNet (HC-ARMNet) model for predictive analytics, optimized by the Improved Secretary Bird Optimization (ISBO) algorithm. The research employs IoT sensors to acquire real-time data, which is then stored in the Ethereum blockchain to ensure security. The HC-ARMNet model, combining 1D/2D convolutions with recurrent connections, processes this data for pest detection and irrigation management. The ISBO algorithm is leveraged to fine-tune the technique's parameters. Datasets used: The proposed system utilizes three standard datasets for evaluation. The PlantifyDr Dataset is used for classifying plant disease, and the Pest Detection Dataset is used for recognizing pests. Also, for the smart irrigation process, the significant field images are collected manually. The accuracy, precision, and FNR rates of the ISBO-HC-ARMNet-aided plant disease classification are 94.16%, 94.2% and 5.87%. At the same time, the ISBO-HC-ARMNet-based pest detection process's accuracy, sensitivity, and specificity are 93.78%, 93.79% and 93.76%, respectively. In addition, the ISBO-HC-ARMNet-based smart irrigation task's MSE is 3.21, SMAPE is 0.03, and MASE is 30.23. Thus, the designed system showcases promising performance over classical approaches in terms of accuracy and error rates for plant disease classification, pest detection, and smart irrigation. The research concludes that the IoT-aided smart farming framework with blockchain and the HC-ARMNet model provides a robust solution for secure and efficient agricultural management. The system's predictive capabilities provide accurate and timely data analysis, facilitating to the improvement of precision agriculture. Future work will focus on improving the system with advanced feature extraction strategies to reduce processing time.
Why it matches plant phenotyping methods植物画像に基づく病害分類モデルの開発・評価が研究の中心的技術貢献であり、感染植物の状態を直接推定しているため含める。
abstractThe study aims to develop a secure and effective IoT-based smart farming framework using the Ethereum blockchain to store and transmit data, and a Hybrid Convolution Adaptive Recurrent MobileNet (HC-ARMNet) model for predictive analytics
Reproduction assets foundThe paper explicitly states that implementation code, trained models, and experimental configurations are publicly available in an authors' GitHub repository, and that the PlantifyDr plant disease dataset and IP02 pest detection dataset used in the study are available on Kaggle. These are paper-specific, public, and可直接Code · publicThe implementation code, trained models, and experimental configurations used are publicly available in: “ https://github.com/sumanthvmani/-Pest-Detection-and-Smart-Irrigation ”. The repository contains all necessary instructions and dependencies required to reproduce the reported experimental results.Open asset ↗https://github.com/sumanthvmani/-Pest-Detection-and-Smart-Irrigationlines:253-302Dataset · publicThe datasets generated and/or analyzed during the current study are available in the [PlantifyDr Dataset and Pest detection dataset] repository“ https://www.kaggle.com/datasets/lavaman151/plantifydr-dataset ”Open asset ↗https://www.kaggle.com/datasets/lavaman151/plantifydr-datasetlines:400-436Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Convolutional Neural Networks (CNNs) are widely used for plant disease detection, yet their performance is strongly influenced by hyperparameter selection. Traditional manual tuning or random search approaches are inefficient and may lead to suboptimal solutions. In this study, we propose and evaluate four hybrid metaheuristic strategies, Ant Lion Optimizer combined with Whale Optimization Algorithm (ALO-WOA), Ant Lion Optimizer with Dragonfly Algorithm (ALO-DA), Ant Lion Optimizer with Particle Swarm Optimization (ALO-PSO), and Particle Swarm Optimization with Whale Optimization Algorithm (PSO-WOA), for automatic hyperparameter tuning of CNNs. The methods were applied to a tomato leaf disease dataset comprising 21,421 training, 4,586 validation, and 4,602 test images across 10 classes. The CNN architecture was fixed with three convolutional blocks and a tunable dropout and learning rate. The experimental results show that ALO-DA achieved the highest test accuracy of 97.83%, closely followed by ALO-WOA (97.67%) and PSO-WOA (97.52%), while ALO-PSO achieved 95.26%. These findings demonstrate that hybrid metaheuristics can effectively improve CNN hyperparameter search compared to single optimizers, balancing exploration and exploitation more efficiently. Limitations and future research directions are discussed.
Why it matches plant phenotyping methodsトマト葉の病害状態を画像から分類するCNNについて、ハイブリッドメタヒューリスティックによるハイパーパラメータ最適化手法を開発・評価しており、植物フェノタイピング手法が中心である。
abstractIn this study, we propose and evaluate four hybrid metaheuristic strategies, Ant Lion Optimizer combined with Whale Optimization Algorithm (ALO-WOA), Ant Lion Optimizer with Dragonfly Algorithm (ALO-DA), Ant Lion Optimizer with Particle Swarm Optimization (ALO-PSO), and Particle Swarm Optimization with Whale Optimization Algorithm (PSO-WOA), for automatic hyperparameter tuning of CNNs.
Reproduction assets foundThe paper uses the public Kaggle Tomato Leaf Disease Dataset V2 as its phenotyping image data and publishes the authors' complete hybrid metaheuristic CNN optimization pipeline (training, optimization, evaluation scripts) in a public GitHub repository. Trained models/logs are only available on request.Code · publicThe source code, trained model configurations, and experimental scripts used in this study are publicly available at: https://github.com/simarkalsi24/Hybrid-of-Optimization-Algorithm-.git The repository includes the complete training and optimization pipeline, implementations of all hybrid algorithms (ALO–DA, ALO–PSO, ALO–WOA, and PSO–WOA), as well as experiment configurations, logs, evaluation scripts, and visualization outputs to ensure full reproducibility of the reported results.Open asset ↗GitHub · simarkalsi24/Hybrid-of-Optimization-Algorithm-lines:579-588Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Soybean production is significantly affected by crop diseases and improper pesticide use, which hinder effective disease management and reduce yield. In this study, we propose an efficient multi-task convolutional neural network (CNN) framework for the simultaneous detection of soybean seed diseases and pesticide presence from seed images. The model leverages a shared feature extraction backbone with task-specific output heads to learn complementary features for both disease classification and pesticide detection. A dataset of 429 soybean leaf images was preprocessed using normalization and augmentation techniques and split into training, validation, and testing sets. We evaluated three backbone architectures VGG19, MobileNetV3, and ConvNeXt within the multi-task framework. Experimental results demonstrate that the approach maintains computational efficiency suitable for real-world deployment while achieving high performance, with accuracies of 95%, 96%, and 97% for MobileNetV3, VGG19, and ConvNeXt, respectively. Additionally, explainable AI methods, such as Grad-CAM, highlight regions of focus for both tasks, making the model's decision-making process interpretable. This framework provides a practical tool for informed crop management and agricultural monitoring.
Why it matches plant phenotyping methods植物画像から病害状態を推定するCNN手法の開発・評価が研究の中心であり、病害表現型の画像ベース推定に該当する。
abstractwe propose an efficient multi-task convolutional neural network (CNN) framework for the simultaneous detection of soybean seed diseases and pesticide presence from seed images.
Reproduction assets foundThe paper's authors publicly released their custom analysis code (preprocessing, training, evaluation) on GitHub, matching an allowed URL. The enriched Kaggle image/annotation dataset is also public but its URL is not among the allowed URLs, so it is not listed.Code · publicThe custom code developed for this study is publicly available on GitHub at https://github.com/fikaduberie/Soybean-Disease-and-Pest (version v1.0).Open asset ↗fikaduberie/Soybean-Disease-and-Pesthtml-lines:1281-1329Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Quantitative disease resistance in plants emerges from complex interactions between host tissues and pathogen growth dynamics, producing a spectrum of phenotypic responses. In plant-fungal interactions, disease is most visibly expressed through lesions that vary in number, size, shape, and color, collectively defining a lesion profile. For Cochliobolus heterostrophus, a fungus causing Southern Corn Leaf Blight of maize (Zea mays ssp. mays), we show that infection on different maize genotypes produces strikingly different lesion profiles. However, it remains unclear whether such macroscopic variation in lesion profiles corresponds to consistent differences in the three-dimensional organization of pathogen colonization within host tissue. We therefore examined variation in the three-dimensional structure of C. heterostrophus-infection networks across host genotypes representing four lesion-profile classes. Using light-sheet microscopy and filament-tracing methods adapted from neuroscience, we developed quantitative metrics to characterize infection network organization, including depth, density, shape, and spatial association with host vascular tissue. In this dataset, network depth was similar across genotypes, whereas network morphology (shape and density), spatial association with vascular bundles, hyphal segment length, and branching frequency varied. Notably, genotypes with similar quantitative resistance levels sometimes exhibited distinct patterns of fungal colonization, suggesting that comparable resistance can arise from different underlying infection dynamics. These findings indicate that lesion profiles may not uniquely predict infection network structure and highlight the utility of three-dimensional network metrics for describing variation that likely reflects multiple underlying host and pathogen processes. This multi-scale framework provides tools for linking macroscopic disease phenotypes with microscopic infection processes in quantitative disease resistance.
Why it matches plant phenotyping methods植物病斑と病原菌感染ネットワークを対象に、ライトシート顕微鏡とトレーシング法を適応し、感染構造を定量化する指標を開発・適用しており、表現型取得法が研究の中心である。
abstractUsing light-sheet microscopy and filament-tracing methods adapted from neuroscience, we developed quantitative metrics to characterize infection network organization, including depth, density, shape, and spatial association with host vascular tissue.
Reproduction assets foundThe paper's Data availability statement explicitly deposits metadata, data, and computer code as Supplementary Files accompanying the open-access publication (Supplementary Materials 1-5, including XLSX datasets and an untyped Supplementary Material 5 likely holding code). These are paper-specific phenotyping assets (eCode · publicMetadata, data, and computer code from this study are available in Supplementary Files included with the publication.Open asset ↗lines:147-204Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Plant leaf diseases must be detected and treated early to improve crop yield and reduce agricultural losses. However, pixel-level representations and the inability to be read limit the applicability of existing deep learning approaches to the agricultural sector. A graph neural network termed the Attention-Enhanced Graph Neural Network (AE-GNN) may explain and diagnose multi-plant leaf disease. The proposed framework models leaf pictures as a graph with nodes representing discriminative leaf areas and edges representing their spatial connection. Before creating the global context vector and classification, graph features are aggregated, and an attention weighting method is applied to refocus on disease-relevant nodes obscured by less informative background characteristics. Final disease prediction uses a multilayer perceptron classifier. A curated dataset of half-spinach and curry leaf pictures is used to assess the proposed method for fifteen illnesses and their healthy classifications. Grad-CAM-based explainable AI methods make the model predictions' most important areas clearer. The dataset and source code from this work are available on GitHub for reproducibility and openness. Experimental results reveal that the proposed AE-GNN outperforms convolutional neural networks and graph-based models in classification. Graph-structured learning, attention enhancement, and explainability create a robust and interpretable framework for multi-plant leaf disease diagnosis.
Why it matches plant phenotyping methods葉画像から植物病害状態を分類・診断する画像解析手法を提案し、既存モデルとの比較評価と説明可能性解析を行っているため、植物フェノタイピング手法が中心である。
abstractA graph neural network termed the Attention-Enhanced Graph Neural Network (AE-GNN) may explain and diagnose multi-plant leaf disease.
Reproduction assets foundThe paper's Data availability section explicitly links a public GitHub repository containing the paper's spinach/curry leaf fungal disease image dataset used for the AE-GNN phenotyping/classification analysis.Dataset · publicData availability
The dataset is available at the link below. https://github.com/MeganathanE1990/FINAL-DISEASE-DATA-SET/tree/mainOpen asset ↗MeganathanE1990/FINAL-DISEASE-DATA-SETlines:413-463Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Pearl millet is a vital crop in arid and semi-arid regions, but its productivity is significantly impacted by fungal and bacterial diseases. Traditional disease detection methods, including manual inspection and conventional machine learning techniques, often suffer from inefficiencies due to subjectivity, time consumption, and limited feature extraction capabilities. Deep learning-based segmentation models such as U-Net, SegNet, DeepLabV3, and FCN have been employed to automate disease identification, but they exhibit limitations in accurately localizing disease-affected regions, handling complex spatial patterns, and maintaining high segmentation precision. To address these challenges, this study proposes HAMNet-Mask R-CNN, an AI-powered hierarchical multi-scale attention network integrated with a spectral-spatial fusion module for precise pearl millet disease detection. The novel framework enhances disease localization by utilizing hierarchical multi-scale feature learning, where low, mid, and high-level features contribute to superior classification accuracy. The attention mechanism prioritizes critical disease regions, reducing false positives, while Mask R-CNN enables pixel-wise segmentation, refining disease boundary identification. The proposed model is implemented using Python and TensorFlow with high-resolution image datasets. Performance evaluation demonstrates HAMNet-Mask R-CNN achieving a 99.35% Dice score, 98.80% IoU, 99.75% Precision, 99.78% Recall, and 99.65% Accuracy, outperforming U-Net (95.03% Dice, 90.53% IoU), SegNet (94.58% Dice, 89.73% IoU), DeepLabV3 (98.31% Dice, 96.69% IoU) and FCN(98.47% Dice score, 97.00% IoU). The results confirm the model's superiority in disease segmentation and classification, offering a robust and scalable solution for real-time disease monitoring in smart agriculture. The integration of hierarchical attention and spectral-spatial fusion significantly enhances detection accuracy, ensuring reliable disease identification and contributing to improved agricultural productivity.
Why it matches plant phenotyping methods真珠粟の病変領域を画像から画素単位で抽出・分類する深層学習手法を開発し、既存モデルと性能比較しているため、植物病害状態のフェノタイピング手法が中心である。
abstractthis study proposes HAMNet-Mask R-CNN, an AI-powered hierarchical multi-scale attention network integrated with a spectral-spatial fusion module for precise pearl millet disease detection.
Reproduction assets foundThe paper's authors explicitly state that the custom HAMNet–Mask R-CNN implementation code (preprocessing, training, evaluation, visualization) is publicly available on GitHub. The pearl millet leaf image dataset used as phenotyping input is also publicly hosted on Roboflow Universe and cited as the data source. The roCode · publicThe custom code developed for the implementation of the proposed HAMNet–Mask R-CNN framework, including data preprocessing, model training, evaluation, and visualization scripts, is publicly available in a GitHub repository. The code can be accessed at: https://github.com/ramyalaksha/Hamnet.gitOpen asset ↗https://github.com/ramyalaksha/Hamnet.gitlines:267-297Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Plant leaf disease detection is a critical task in precision agriculture, where reliable diagnosis under real-world conditions is essential for reducing crop losses and supporting timely intervention. Although deep learning models have achieved high classification accuracy, their performance often degrades under domain shift between controlled laboratory datasets and real-field environments, while predictive uncertainty and confidence calibration remain largely unaddressed.This study presents an uncertainty-aware cross-domain evaluation framework based on a Hierarchical Vision Transformer (HViT) for plant leaf disease classification. The framework integrates multi-scale feature learning with Monte Carlo Dropout-based predictive uncertainty estimation and temperature-based calibration to systematically analyze model behavior in terms of accuracy, reliability, and robustness. Experiments were conducted on two complementary datasets: the New Plant Diseases Dataset (controlled conditions) and the PlantDoc dataset (field conditions), enabling bidirectional cross-domain evaluation. Results demonstrate that the proposed framework achieves superior performance, attaining 97.8% accuracy on controlled data and 93.6% on field data, while significantly improving calibration with lower Expected Calibration Error (ECE = 0.032 / 0.041), reduced Negative Log-Likelihood, and lower Brier score compared to baseline CNN and transformer models. Furthermore, the framework exhibits improved robustness under domain shift, with reduced performance degradation and stable uncertainty behavior. Overall, this study highlights the importance of integrating uncertainty estimation and calibration within a hierarchical transformer-based framework, providing a more reliable and deployment-ready solution for real-world agricultural disease diagnosis.
Why it matches plant phenotyping methods植物葉の病害状態を直接推定する不確実性-aware分類フレームワークの開発・評価が中心であり、異なる条件のデータセット間で精度、校正、頑健性を検証している。
abstractThis study presents an uncertainty-aware cross-domain evaluation framework based on a Hierarchical Vision Transformer (HViT) for plant leaf disease classification.
Reproduction assets foundThe paper's Data availability statement explicitly links the two public image datasets used for its cross-domain plant leaf disease classification experiments: the New Plant Diseases Dataset on Kaggle and the PlantDoc dataset on Dataset Ninja. No author analysis code, models, or checkpoints are reported as available.Dataset · publicThe New Plant Diseases Dataset can be obtained from Kaggle at [https://www.kaggle.com/datasets/vipoooool/new-plant-diseases-dataset]Open asset ↗Kaggle · vipoooool/new-plant-diseases-datasetlines:360-398Dataset · publicThe PlantDoc dataset is available for download at [https://datasetninja.com/plantdoc#download]Open asset ↗plantdoclines:360-398Code / dataset availability confirmedEurope PMC · Crossref · checked 5 Sept 2026
Plant diseases pose a major threat to global food security, significantly reducing agricultural yields. Therefore, timely diagnosis of plant diseases can help prevent food losses and support economic stability. This study explores the use of eight Convolutional Neural Networks and two Vision Transformers for apple leaf disease diagnosis. The feature extraction layers of each model were modified to incorporate DropBlock layers, while preserving pretrained weights from the ImageNet dataset. Images from the Plant Pathology 2021 dataset were used to fine-tune the models for multi-label classification, targeting five disease categories and a healthy label. Three experiments were conducted to evaluate model performance on the test set. First, the ResNet50 model was used to determine optimal Dropout and DropBlock probabilities. Second, these parameters were applied across all models to identify those with the best performance. Finally, twenty-three Swarm Optimization Algorithms were used to optimize classifier thresholds, improving accuracy and F1-scores. A DropBlock probability of 0.05 and a Dropout probability of 0.2 yielded superior results. Among the models, SwinV2T attained an accuracy of 90.7%, while SwinV2S achieved the highest F1-score of 91.7%, slightly outperforming the ConvNeXtT and ConvNeXtS architectures. The results demonstrated the effectiveness of DropBlock regularization and optimized classifier thresholds, highlighting the superior performance of recent architectures and optimization algorithms over their older counterparts. These findings suggest that such networks hold substantial promise for accurately identifying and diagnosing apple leaf diseases.
Why it matches plant phenotyping methodsリンゴ葉画像から病害状態を分類・診断する深層学習手法を比較評価し、正則化や閾値最適化による性能改善も検証しており、植物フェノタイピング手法が研究の中心である。
abstractThis study explores the use of eight Convolutional Neural Networks and two Vision Transformers for apple leaf disease diagnosis.
Reproduction assets foundThe paper's apple leaf disease phenotyping is based on the public Plant Pathology 2021 (FGVC8) Kaggle image dataset and an authors' reorganized multi-label version publicly deposited on GitHub; both are explicitly linked in the Data availability statement. No author analysis code or trained model checkpoints are statedDataset · publicFor this research, the dataset was reorganized and extended into a multi-label format. The complete modified dataset is publicly available at: https://github.com/soroushtou/Plant-Pathology-2021---MultiLabel-Dataset.Open asset ↗Plant-Pathology-2021---MultiLabel-Datasetlines:239-262Dataset · publicThe original dataset used in this study is the publicly available Plant Pathology 2021 dataset from the FGVC8 competition available at: https://www.kaggle.com/competitions/plant-pathology-2021-fgvc8.Open asset ↗lines:239-262Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The increasing global food insecurity driven by climate-induced natural hazards and soil degradation has made the resilience of alternative agricultural systems a critical focus in risk management. This study presents a geospatially integrated monitoring framework, the Optimized Multi-Scale Adaptive Graph Neural Network (OMSA-GNN), designed to mitigate risks associated with nutrient instability in hydroponic and aeroponic environments. The proposed system leverages a Raspberry Pi-based IoT network to monitor complex interactions among microclimatic variables, plant physiological health, and nutrient concentrations, treating them as localized geospatial data points. To enhance decision-making under environmental uncertainty, an Improved Sparrow Search Algorithm (ISSA) is employed to optimize the predictive performance of the GNN. The OMSA-GNN model incorporates visual plant indices as a proximal remote sensing approach to enable early detection of physiological stress that may lead to crop failure. Evaluated using a lettuce growth dataset, the framework demonstrates superior performance in forecasting growth trajectories and managing resource-related risks compared to conventional static models. The results highlight a scalable approach for improving the reliability of urban food systems, where traditional land-based agriculture is increasingly vulnerable to natural hazards.
Why it matches plant phenotyping methods植物の生理的ストレスと成長軌跡を、視覚的植物指数およびIoTセンサーデータから推定するGNNベースの監視・解析手法が研究の中心であり、植物表現型取得と予測に該当する。
abstractThe OMSA-GNN model incorporates visual plant indices as a proximal remote sensing approach to enable early detection of physiological stress that may lead to crop failure.
Reproduction assets foundThe paper's Data Availability statement points to a public Kaggle lettuce growth dataset used for evaluation, matching an allowed URL. No author code or model checkpoints are disclosed.Dataset · publicThe datasets used and/or analyzed during the current study are available in the Kaggle repository, https://www.kaggle.com/datasets/jurijsruko/lettuce/data.Open asset ↗Kaggle · jurijsruko/lettucehtml-lines:469-500Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The research proposes Cross Disease Similarity Awareness Learning (CDSAL), a robust multiclass tomato leaf disease detection framework based on high-quality and explainable deep learning. The approach solves the problem of superimposed patterns of disease especially Leaf Miner, Tomato Spotted Wilt Virus (TSWV), and nutrient deficiencies through the combination of multi-domain feature learning and inter-disease similarity modeling. In contrast to conventional metric learning or contrastive learning methods that function on pairwise or triplet sample associations, CDSAL develops a class-level Cross Disease Similarity Matrix that represents structured inter-disease proximity within the embedding space. Moreover, rather than employing episodic prototype construction typical of few-shot learning, the proposed system persistently updates centroid representations throughout supervised training and incorporates similarity-aware regularization directly into the loss function. This facilitates structural embedding reshaping specifically designed for visually overlapping illness categories, beyond traditional prototype-based learning methodologies. The input images are processed through HSV based green masking, morphological cleaning, extraction of leaf contours and resizing, and using a large amount of geometric and color-space augmentation to reduce the imbalance among the classes. DenseNet121 and EfficientNet-B0 are used to obtain feature representations and class-separated centroid of latent embedding's to form a Cross Disease Similarity Matrix, where similarity-aware optimization is possible during training. Grad-CAM on the target layers offers decipherable disease-specific activation signatures. The findings of the experiments show that classification accuracy at unseen samples is 99.77% with high resilience to visual confounding. The predictions, proximity of diseases that are similar and explainable features are provided by CDSAL, thereby facilitating reliable decision-making in agricultural diagnostics.
Why it matches plant phenotyping methodsトマト葉の病害状態を画像から推定する深層学習手法を提案し、前処理・特徴抽出・類似度学習・説明可能性を技術的中心として評価しているため。
abstractThe research proposes Cross Disease Similarity Awareness Learning (CDSAL), a robust multiclass tomato leaf disease detection framework based on high-quality and explainable deep learning.
Reproduction assets foundThe paper's plant-phenotyping inputs are two publicly available Kaggle image datasets explicitly named in the Data Availability statement: PlantVillage (emmarex/plantdisease) used as the main dataset and TomatoVillage (mamtag/tomato-village) used for ablation/field-condition experiments. No author analysis code, modelsDataset · publicThe datasets analyzed during the current study are available in the Kaggle repository. [https://www.kaggle.com/datasets/emmarex/plantdisease]Open asset ↗Kaggle · emmarex/plantdiseasehtml-lines:605-624Code / dataset availability confirmedCrossref · Europe PMC · checked 15 Sept 2026
Abstract Plant diseases are a serious danger to the world’s food security, because they lower agricultural output and increase economic losses. Due to subjectivity, fluctuating lighting, and environmental unpredictability, traditional visual examination techniques are frequently incorrect. The Excess Green (ExG) vegetation index and pseudo-thermal representations produced from RGB pictures are two synthetically developed complementary representations that are integrated with RGB imagery in this study’s lightweight multimodal deep learning system to address these issues. Histogram shifting and pseudo-infrared color mapping are used in a reproducible picture alteration pipeline to create the pseudo-thermal modality, which allows for extra visual signals without the need for specific thermal sensors. In order to classify plant diseases while preserving computational efficiency, the suggested framework uses MobileNetV3-Small backbones to extract modality-specific characteristics. This is followed by feature-level fusion. The publicly accessible Ginger Leaf Dataset, which includes RGB pictures of ginger leaves in four different conditions—Damage-Pest, Dehydrated, Healthy, and Leaf-blight—was used for the experiments. For training, validation, and testing, the dataset was split using a stratified 70:15:15 split. Python-based preprocessing procedures were used to create the extra modalities (ExG and pseudo-thermal representations) from the original RGB images. The experimental results show that the combination of the representations with RGB images can enhance the classification performance compared with the unimodal RGB-based models. Ablation experiments are also conducted to examine the contributions of different modalities to the overall categorization accuracy. The experimental results show that plant disease recognition can be improved with the help of efficient computing by combining lightweight convolutional neural networks with computationally generated visual representations.
Why it matches plant phenotyping methodsRGB画像からExG・疑似熱画像を生成し、植物葉の病害状態を分類するマルチモーダル手法が研究の中心であり、アブレーション評価も実施している。
titleHybrid deep learning-based multimodal framework for plant leaf disease classification using RGB, Excess Green (ExG), and pseudo-thermal representations with MobileNetV2
Reproduction assets foundThe paper's phenotyping experiments use the publicly available Ginger Leaf Dataset (RGB leaf images of four ginger leaf conditions), with a public GitHub repository and dataset website. The authors' derived ExG/pseudo-thermal representations and preprocessing scripts are only available upon request, so they do not yetDataset · publicor multispectral images
IEEE Geosci. Remote Sens. Lett. 2025
10.1109/LGRS.2025.XXXXXXX
Ulku, I., Tanriover, O. O. & Akagündüz, E. Cross-band correlation-aware interactive fusion for multispectral images. IEEE Geosci. Remote Sens. Lett.
10.1109/LGRS.2025.XXXXXXX
(2025).
10. Wong, J. Ginger Leaf Dataset. GitHub Repository (2023). https://github.com/wongjay1941/Ginger-Leaf-Dataset
11.
Bhakta I
A novel plant disease prediction model based on thermal images using modified deep convolutional neural network
Precis. Agric. 2023 24 23 39
10.1007/s11119-022-09927-x
Bhakta, I. et al. A novel plant disease prediction model based on thermal images using modified deep convolutional neural network. Precis.Open asset ↗https://github.com/wongjay1941/Ginger-Leaf-Datasetlines:580-681Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Many countries greatly rely on agriculture as a means of livelihood and economic growth. Even the most industrialized countries need food, medicine, clothing, and shelter produced by crops. Rice is one of the most significant and widely grown crops worldwide. Nonetheless, the severely impacted crops in rice production are those of bacteria, fungi, and viruses, which decrease yield and quality. Manual disease detection is hectic, challenging, and, in most cases, inaccurate. Recent advances in deep learning and computer vision have demonstrated significant potential to improve the detection and classification of diseases. This study proposes a deep learning hybrid model for the automated detection and classification of rice leaf diseases. This method consists of five key stages: image preprocessing, segmentation, augmentation, multi-feature extraction via adaptive fusion, and classification. There are five rice leaf diseases to discuss and recognize: Blight, brown spot, sheath blight, tungro, and leaf blast. The first step is global contrast enhancement, which improves image quality. After that, the segmentation is performed using Otsu's Thresholding to extract the leaf area. Then, the modified VGG16 and modified ResNet50 networks are used in parallel to extract features using a transfer-learning approach. The adaptive fusion technique combines these features to obtain a dominant, proper feature representation. Lastly, the classification is done using an adaptive fusion score technique. Experimental results show excellent performance, with class-wise Precision in the range of 95.5-100%, class-wise recall in the range of 97.4-100%, and overall test accuracy of 98.5%.
Why it matches plant phenotyping methodsイネ葉の病害状態を画像から自動検出・分類する深層学習ワークフローが研究の中心であり、葉領域抽出、特徴抽出、分類性能まで評価しているため、植物病害フェノタイピング手法に該当する。
abstractThis study proposes a deep learning hybrid model for the automated detection and classification of rice leaf diseases.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe dataset employed in this study is accessible online at https://www.kaggle.com/datasets/rajeshbhattacharjee/rice-diseases-using-cnn-and-svm.Open asset ↗Kaggle · rajeshbhattacharjee/rice-diseases-using-cnn-and-svmhtml-lines:929-951Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Today, the intelligent automation of agriculture has received much attention from researchers. One of the important factors for the success of this automation is the timely diagnosis of plant disease and making a decision appropriate to the existing conditions of the plant. Since the progress of the disease is a determining factor in the type of treatment method, the diagnosis of the severity of the disease is of particular importance. However, accurate diagnosis of plant disease progression depends on various factors, including the availability of appropriate and well-annotated training datasets for designing an efficient diagnostic system. On the other hand, the similarity of the complications of different diseases has made this work challenging. In this study, two tomato diseases, namely Bacterial Spot and Mosaic Virus, are investigated using images collected from the PlantVillage, Taiwan tomato leaves, Field-PlantVillage, and Syn-PlantVillage datasets. The disease severity levels are divided into six stages for Bacterial Spot and four stages for Mosaic Virus, and a specifically designed deep convolutional neural network is proposed for severity classification. Experimental results demonstrate that the proposed method achieves high accuracy under challenging field conditions and outperforms several state-of-the-art methods.
Why it matches plant phenotyping methodsトマト葉の病徴・病害重症度を画像から段階分類するCNN、背景除去、病斑セグメンテーションを開発しており、植物状態の取得・推定手法が中心である。
titleA parallel convolutional neural network with background removal and lesion segmentation for field plant disease severity classification
Reproduction assets foundThe paper's own severity-annotated datasets are explicitly restricted (available only on request), so no public paper-specific data asset qualifies. The authors do provide an explicit public code availability link for their proposed BaSPaC model. The Mendeley and Drive links are pre-existing external datasets cited as,Code · publicCode availability
https://github.com/m-hasheminejad/BaSPaC.Open asset ↗m-hasheminejad/BaSPaChtml-lines:878-908Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The citrus diseases are affecting the fruit production worldwide thereby posing an economical burden. Major research is moving towards finding solutions using Artificial Intelligence (AI) and Image processing methods. Due to factors like illumination variations, leaf form, and disease symptoms, image data has intrinsic uncertainties that are typically difficult for traditional machine learning techniques to handle. In this paper, the interpretability of fuzzy logic is combined with the resilience of deep learning to propose a novel Fuzzy Convolutional Neural Network (Fuzzy-CNN) architecture for the automated diagnosis of citrus leaf diseases. The hybrid method uses a Convolutional Neural Network (CNN) to obtain complex features of citrus images, and a Fuzzy Inference System (FIS) to improve the classification results. The proposed approach encodes accurate data into fuzzy sets and applies linguistic concepts to determine the severity of a disease, which will contribute to the further development of the decision. In order to test and verify the proposed approach, several experiments were carried out, which proved that Fuzzy-CNN is more effective than regular CNN models with the approximate accuracy difference approximately 1.8, and especially in cases when the symptoms of disease are not clear. To strengthen experimental validation, the proposed method is evaluated on two independent datasets, including an external benchmark dataset, imbalance-aware evaluation metrics are employed to ensure robustness and generalizability. Experimental results demonstrate consistent and statistically significant improvements over existing neuro-fuzzy and machine learning approaches. This research contributes to early detection by collaborating the potential of fuzzy neural networks and offering a flexible solution for real-time disease detection in citrus crops.
Why it matches plant phenotyping methods柑橘葉画像から病害および重症度を推定するFuzzy-CNN手法を開発し、独立データセットとベンチマークで検証しており、植物フェノタイピング手法が中心である。
abstractpropose a novel Fuzzy Convolutional Neural Network (Fuzzy-CNN) architecture for the automated diagnosis of citrus leaf diseases.
Reproduction assets foundThe paper's Data Availability statement links two public image datasets used for the citrus disease phenotyping/classification experiments (a Mendeley citrus leaves dataset and a Kaggle orange fruit dataset), and a third public Kaggle citrus disease dataset is cited as the external benchmark dataset used for validationDataset · publicThe data used in the current study is publicly available from the following links. [https://data.mendeley.com/datasets/3f83gxmv57/2]Open asset ↗data.mendeley.com · 3f83gxmv57/2html-lines:525-539Dataset · publicThe data used in the current study is publicly available from the following links. [https://data.mendeley.com/datasets/3f83gxmv57/2] [https://www.kaggle.com/datasets/sgandhi2003/orange-fruit-dataset]Open asset ↗www.kaggle.com · sgandhi2003/orange-fruit-datasethtml-lines:525-539Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
This paper explores the use of optimized convolutional neural networks (CNNs) to classify diseases affecting potato leaves using TensorFlow-2. The dataset, sourced from Kaggle's Plant Village repository, includes 152 images of healthy potato leaves and 1000 images each of early and late blight. The methodology covers data preparation, model architecture design, training, evaluation, and deployment. During data preparation, the data set was split into training sets (80%) and testing sets (20%), with images resized to 128x128 pixels. The Deep Learning (DL) models built using CNN with 4 different optimizers (ADAM, SGD, RMSPROP, and ADAMAX) and trained using a sparse categorical cross-entropy loss function, include multiple convolutional and pooling layers for feature extraction, and fully connected layers for classification. Early stopping was used to prevent overfitting. Model performance was assessed using accuracy, loss curves, confusion matrix, ROC curve, precision recall curve, classification report, and F1 score. In addition, we have used data augmentation to balance the dataset by increasing healthy potato leaves 6 times and the use of Ensemble Deep Learning (EDL). EDL10 which contains DL1 (CNN + ADAM), DL2 (CNN + SGD), DL3 (CNN + RMSPROP) and DL4 (CNN + ADAMX) performs best with a accuracy score of 97.0%. This highlights the importance of data balancing and the use of the ensemble classification approach for the detection of blight in Potato Leaves.
Why it matches plant phenotyping methodsジャガイモ葉の病害状態を画像から分類するCNN・アンサンブル手法の設計、評価、データ拡張が研究の中心であり、植物病害フェノタイピング手法に該当する。
abstractThis paper explores the use of optimized convolutional neural networks (CNNs) to classify diseases affecting potato leaves using TensorFlow-2.
Reproduction assets foundThe paper uses the public Kaggle PlantVillage potato leaf image dataset and archives its complete analysis source code on Zenodo with explicit availability statements and URLs.Code · publicThe complete source code is hosted in a DOI-minting repository and has been archived on Zenodo to ensure long-term accessibility and reproducibility. The code is released under an open-source license. The archived version corresponding to this publication is available at : https://doi.org/10.5281/zenodo.19624017Open asset ↗Zenodo · 10.5281/zenodo.19624017lines:252-314Dataset · publicThe datasets generated and/or analysed during the current study are available at : PlantVillage Dataset, accessed from https://www.kaggle.com/datasets/abdallahalidev/plantvillage-dataset.Open asset ↗Kaggle · plantvillage-datasetlines:252-314Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Crop diseases pose a significant threat to agricultural productivity and global food security. Timely and accurate detection of such diseases is crucial for improving both crop yield and quality. While numerous deep learning approaches rely solely on image data for disease identification, they often overlook the complementary value of textual information in enhancing visual analysis. To address this limitation and effectively fuse features from different modalities, we propose a Cross-Model fusion framework based on a vision-language model that integrates cross-attention and gated fusion mechanisms for crop disease recognition. Our approach utilizes the Zhipu.ai multi-modal model to generate comprehensive textual descriptions of diseased crop leaves, including global description, local lesion description, and color-texture description. These textual descriptions are then encoded into feature embeddings, while visual features are extracted using the ShuffleNet-v2 model as the image encoder. Subsequently, a cross-attention module aligns and fuses the two modalities, and a gated fusion module enables dynamic feature selection during the fusion process. Extensive evaluations on the Soybean Disease and PlantVillage datasets demonstrate that our method outperforms existing image-based models in terms of accuracy. Specifically, our model achieves recognition accuracies of 99.04% and 99.12% on the respective datasets, surpassing the ShuffleNet-V2 model by 1.09% and 2.53%, respectively. These results highlight the effectiveness of Cross-Model learning in integrating visual and textual cues for accurate and efficient disease recognition, offering a scalable solution for crop disease diagnosis.
Why it matches plant phenotyping methods植物葉の病徴を画像と言語情報から認識する融合フレームワークを開発し、複数データセットで既存手法と比較評価しているため、植物フェノタイピング手法が中心である。
abstractwe propose a Cross-Model fusion framework based on a vision-language model that integrates cross-attention and gated fusion mechanisms for crop disease recognition.
Reproduction assets foundThe paper's crop disease recognition experiments use two openly available image datasets, both with explicit public availability statements in the Data Availability section: the Soybean Disease dataset (Dryad DOI) and the PlantVillage dataset (Kaggle). No author analysis code, trained models, or generated text-annotaitDataset · publicThe datasets utilized in this study are openly accessible. The soybean dataset is available at https://doi.org/10.5061/dryad.41ns1rnj3.Open asset ↗Dryad · 10.5061/dryad.41ns1rnj3html-lines:403-424Dataset · publicThe plantvillage dataset is available at https://www.kaggle.com/datasets/abdallahalidev/plantvillage-dataset.Open asset ↗Kaggle · plantvillage-datasethtml-lines:403-424Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Abstract Timely and accurate plant disease detection is important for enhancing agricultural productivity and promoting sustainability. The study introduces Multimodal Adaptive Fuzzy-based Deep Neural Network (MAF-DNN) for classification of plant diseases. The proposed method combines fuzzy logic with multimodal data fusion to effectively address the complex interactions and uncertainties in agricultural datasets. The MAF-DNN employs a robust adaptive fuzzy framework with dynamic rule optimization and integrates Hyperspectral Imaging Data (HID) with RGB imaging data to acquire detailed spectral information and high-resolution visual cues for disease classification. The multimodal fusion enhances the model’s ability to capture intricate patterns that relate to plant health, improving the accuracy of disease classification. The experimental results showed that the MAF-DNN outperforms traditional models by achieving an accuracy of 97.8%, precision of 96.5%, recall of 98.2%, and F1-score of 97.3%. Additionally, the adaptive design reduces computational overhead, increases efficiency, and improves scalability for large-scale agricultural applications. The MAF-DNN represents a significant advancement in plant disease classification and provides a robust and efficient solution for precision agriculture.
Why it matches plant phenotyping methods植物病徴を画像から分類するマルチモーダル画像・深層学習手法の開発と性能評価が中心であり、植物の病害状態を直接推定するため。
abstractThe study introduces Multimodal Adaptive Fuzzy-based Deep Neural Network (MAF-DNN) for classification of plant diseases.
Reproduction assets foundThe paper uses two public Kaggle plant disease image datasets (New Plant Diseases Dataset and CCMT Plant Disease Dataset) as its phenotyping inputs and states that the authors' custom MAF-DNN code is publicly available on GitHub, with all three URLs given in the article and matching allowed URLs.Code · publicThe custom code used to develop and evaluate the proposed Multimodal Adaptive Fuzzy Deep Neural Network (MAF-DNN) framework is publicly available at: https://github.com/skbsangeetha/MAF-DNN-Plant-disease-classificationOpen asset ↗skbsangeetha/MAF-DNN-Plant-disease-classificationhtml-lines:102-118Code / dataset availability confirmedOpenAlex · Europe PMC · checked 5 Sept 2026
Accurate three-dimensional (3D) nuclear instance segmentation is a prerequisite for quantitative phenotyping in volumetric microscopy, yet remains challenging in densely packed tissues, irregular nuclear morphologies, and across heterogeneous imaging modalities. Here we present NucVerse3D, a deep-learning framework for generalized 3D nuclei instance segmentation that combines a residual attention 3D U-Net architecture with a reversible gradient-field representation for robust centroid-aware instance reconstruction. NucVerse3D is trained end to end in 3D using modality-agnostic preprocessing and isotropic scale normalization, enabling deployment across confocal microscopy, two-photon microscopy, light-sheet microscopy, micro-computed tomography, and scanning electron microscopy volumes. We benchmarked NucVerse3D on seven volumetric datasets spanning multiple species and tissues, comprising more than forty thousand manually annotated nuclei, including newly released ground-truth datasets of mouse liver tissue (control and hepatocellular carcinoma) and Drosophila brain glial nuclei. Across datasets, NucVerse3D achieved consistently high precision and competitive recall, resulting in strong F1-scores and average precision across a wide range of imaging conditions. While a modest precision-recall imbalance is observed in certain datasets, favoring high-confidence detections, this behavior reflects a conservative instance reconstruction strategy that prioritizes accurate boundary delineation and reduces false positive segmentation in densely packed and morphologically heterogeneous tissues. A single generalized model trained on pooled data matched the performance of dataset-specific models, and ablation experiments demonstrated that preprocessing and scale normalization substantially contribute to performance under strict intersection-over-union criteria. To demonstrate the biomedical utility of NucVerse3D, we applied it to 3D liver images from a mouse model of hepatocellular carcinoma (HCC) to enable spatially resolved 3D nuclear phenotyping. In healthy liver tissue, nuclear DNA content and nuclear volume exhibited a tightly regulated log-log scaling relationship. In contrast, tumor-adjacent and tumor regions displayed progressive disruption of this coupling, forming spatially coherent domains of nuclear DNA-volume decoupling that are not detectable in conventional two-dimensional histology. We quantify this phenomenon using a Nuclear Decoupling Score (NDS), revealing increased nuclear instability aligned with pathological tissue remodeling highlighting NDS as a potential quantitative biomarker of dysplastic and tumor tissue. Together, NucVerse3D provides a robust and generalizable solution for 3D nuclear instance segmentation and enables quantitative nuclear phenotyping across imaging modalities.
Why it matches plant phenotyping methods3D核セグメンテーション手法を開発し、多様な画像データセットでベンチマーク・検証したうえで、核形態とDNA量の定量的フェノタイピングに応用しており、植物対象ではないため本索引の対象外となる可能性はあるが、提示内容上はフェノタイピング手法研究として中心的である。
abstractAccurate three-dimensional (3D) nuclear instance segmentation is a prerequisite for quantitative phenotyping in volumetric microscopy
Reproduction assets foundThe paper's newly released Zenodo deposit (10.5281/zenodo.18517324) containing raw volumes, annotations, training patches, model weights, and segmentation outputs is not among the allowed URLs, so it cannot be listed. The authors' public analysis/segmentation code repository is explicitly deposited with an authors' URLCode · publicThe source code for training and predicting nuclei segmentation using NucVerse 3D is available from https://github.com/Segovia-lab/3D-Nuclei-segmentation.git .Open asset ↗https://github.com/Segovia-lab/3D-Nuclei-segmentation.gitlines:647-728Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Climate change-induced drought increasingly constrains water management in mixed-species urban gardens, requiring scalable and non-destructive approaches. This study proposes an integrated framework combining chlorophyll fluorescence, RGB image indices, and machine learning to classify plant drought response patterns. Ten garden plant species were evaluated under varying soil moisture conditions. Hierarchical cluster analysis integrating fluorescence parameters and RGB indices identified three physiologically defined response clusters, and their reproducibility using RGB indices alone was assessed. A total of 1,629 samples were augmented to 1,881 using the synthetic minority over-sampling technique (SMOTE) to address class imbalance. A support vector machine (SVM) model with a radial basis function kernel, using green leaf index (GLI), normalized green-red difference index (NGRDI), blue-green pigment index (BGI), and soil moisture (%) as predictors, achieved an accuracy of 0.91 and a Kappa coefficient of 0.84. In contrast, PLS-DA showed lower performance (accuracy 0.79, Kappa 0.65), indicating limited separability under linear assumptions. These results demonstrate that RGB indices combined with nonlinear models were able to reproduce physiologically defined drought response patterns under the given conditions. As a proof of concept, this study demonstrates the potential of the proposed framework; however, its generalizability is limited by the controlled greenhouse setting, the relatively small number of species, and the lack of external validation in heterogeneous field environments. The framework may provide a cost-effective approach for classifying plant drought responses and has the potential to support the grouping of plants with similar water requirements, which could contribute to improved irrigation management in mixed-species gardens under further validation.
Why it matches plant phenotyping methodsRGB画像指標と機械学習により、植物の干ばつ応答パターンという生理状態を分類し、蛍光測定との再現性を評価しているため、表現型取得・抽出手法が中心です。
abstractThis study proposes an integrated framework combining chlorophyll fluorescence, RGB image indices, and machine learning to classify plant drought response patterns.
Reproduction assets foundThe paper explicitly states that the authors' analysis code (data processing, feature extraction, SVM/PLS-DA modeling) is publicly deposited on Zenodo with a DOI matching an allowed URL. The phenotype datasets are only said to be in the manuscript/supplementary files, so the code deposit is the qualifying paperSpecificCode · publicThe code supporting the findings of this study, including data processing, feature extraction, and machine learning modeling is available at Zenodo: https://doi.org/10.5281/zenodo.19127295 .Open asset ↗Zenodo · 10.5281/zenodo.19127295lines:98-116Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Field / plotLeafClassificationSegmentationStress / disease detectionDisease symptoms / severity
Early and accurate detection of plant leaf diseases is an essential requirement for precision agriculture, given their severe impact on global food security. While much has been done recently, many deep learning-based approaches will still fail in real-world tests because of challenges such as background clutter, differences in illumination, occlusion, or the fact that visual symptoms for these diseases can be very subtle early on. Traditional CNN- and Transformer-based architectures generally lack accurate lesion localisation and interpretability, hindering their practical deployment in agricultural decision-support tools. To address these issues, we present LDDHybridNet, a region-based, explanation-friendly deep learning framework that can identify leaf disease at an early, accurate stage. It then applies preprocessing steps guided by ROI, based on leaf segmentation from the U-Net, followed by a compact CNN-based spatial feature-extraction framework. We arrange spatial feature embeddings extracted from lesion regions into an ordered sequence and employ a Bi-LSTM with attention to model structured contextual dependencies, allowing progression-aware feature learning without requiring actual temporal image sequences. Lastly, Grad-CAM-based post-hoc explainability is employed to interpret model decisions, enabling transparent visualisation of disease-relevant regions. We conduct extensive experiments on the PlantVillage benchmark and the FieldPlant dataset and show that LDDHybridNet consistently outperforms representative CNN, transformer, and hybrid baselines across multiple evaluation metrics. Although the near-ceiling performance on PlantVillage reveals the dataset's artificial nature, the proposed framework achieves 95.37% accuracy under real-world field conditions and 92.84% on weak-lesion early-stage samples, demonstrating the method's robustness and early-stage detection potential. The performance boosts are statistically significant (P < 0.01). In general, LDDHybridNet is an interpretable and robust deep learning framework for leaf disease detection, which can support data-driven crop protection and precision agriculture applications.
Why it matches plant phenotyping methods葉の病害症状を画像から検出・局在化する深層学習手法の開発とベンチマーク評価が中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として収録する。
abstractwe present LDDHybridNet, a region-based, explanation-friendly deep learning framework that can identify leaf disease at an early, accurate stage.
Reproduction assets foundThe paper's phenotyping measurements are leaf disease detection experiments on two public image datasets: PlantVillage (Kaggle) and FieldPlant (IEEE Dataport), both cited with explicit public URLs. The authors' code, trained weights, and scripts are not publicly released and are available only on request, so no code/模型Dataset · public43.Hughes, D. P. & Mohanty, S. P. PlantVillage Dataset. [online] (2015). Available at: https://www.kaggle.com/datasets/emmarex/plantdiseaseOpen asset ↗PlantVillage Datasethtml-lines:657-726Dataset · public44.Moupojou, R. K., Bouachir, W., Ahamed, T. & Taki, A. H. FieldPlant: A Real-World Dataset for Leaf Disease Detection in Field Conditions. IEEE Dataport. [online] (2021). Available at: https://ieee-dataport.org/documents/fieldplant-datasetOpen asset ↗FieldPlanthtml-lines:657-726Code / dataset availability confirmedCrossref · Europe PMC · checked 15 Sept 2026
Proper diagnosis of crop diseases and accurate measurement of fruit ripeness is essential in enhancing agricultural productivity, but conventional methods of diagnosis are time-consuming, error-prone, and inefficient. With the rapid development of AI, deep learning (DL), and IoT, there is increasing demand for combined solutions that jointly address plant health monitoring and harvest optimization in a reproducible and deployment-oriented manner. This study develops a new bi-phasic DL framework, AgroDualNet, that predicts crop diseases and identifies fruit ripeness stages to optimize yield quality and minimize agricultural losses. The work explicitly targets improved classification reliability, broader class evaluation, rigorous validation and generation of decision-ready outputs for precision agriculture. AgroDualNet comprises two modules. The crop-disease prediction module integrates ResNet50 with a Convolutional Block Attention Module (CBAM), and a Sequential Minimal Optimization (SMO)-based SVM classifier to enhance feature learning and classification performance Several different architectural designs are benchmarked and the resultant model is tested on both a dedicated 3-class subset and a large multi-class model of the PlantVillage dataset with leakage safe protocol(augmentation applied only on training data), cross-validation, statistical significance testing as well as ablation. The fruit-ripeness module employs YOLOv8 for real-time fruit localization and MobileNetV2 for lightweight ripeness classification suitable for edge deployment and a prototype decision-support layer maps predictions to actionable recommendations. That is able to run on the edge. Experiments show that the hybrid CBAM + ResNet50 + SMO model achieves 99.6% accuracy for crop disease classification on a three-class configuration of the PlantVillage dataset and maintains consistently higher accuracy than strong baseline in a 38-class setting, with statistically significant results confirmed by McNemar's test (p < 0.001) outperforming baseline and intermediate architectures in accuracy, precision, Recall and F1-Score The fruit ripeness pipeline achieves 98.88% classification accuracy across four ripeness stages (unripe, semi-ripe, ripe, over-ripe) on a combined Kaggle and real-field apple dataset with low inference time, confirming its suitability for near real-time deployment on edge devices. Cross-validation, Statistical significance tests and ablation studies collectively validate the robustness and significance of these gains and the decision-support layer demonstrates the feasibility of converting raw predictions into interpretable, recommendation-oriented outputs. AgroDualNet provides an efficient and unified system for monitoring plant diseases and evaluating fruit ripeness, with statically validated performance across both focused and full multi-class settings, addressing two critical challenges in precision agriculture with a single extensible framework. The dual-module design of AgroDualNet, which combines disease prediction with ripeness analysis and a preliminary decision-support prototype offers a more comprehensive and practically relevant AI-driven monitoring solution than conventional single-task models. By emphasizing multi-class validation on PlantVillage, leakage-aware experimentation, statistical verification, and system-level integration, this works supports real-time, precise and automated guidance to reduce crop losses, improve harvest timing, and enable smarter farm-level decision making.
Why it matches plant phenotyping methods植物病害状態と果実成熟度を画像から推定する深層学習パイプラインの開発・比較検証が中心であり、PlantVillageおよび実圃場データで交差検証、アブレーション、統計検定を実施しているため、植物フェノタイピング手法として含める。
abstractThis study develops a new bi-phasic DL framework, AgroDualNet, that predicts crop diseases and identifies fruit ripeness stages
Reproduction assets foundThe paper's Data availability statement names two public datasets used directly in the phenotyping experiments: the PlantVillage crop-disease dataset and a Kaggle apple fruit-ripeness dataset, both with explicit Kaggle URLs matching allowed_urls. The statement also mentions implementation files, trained weights, and a Dataset · public. and V.V. wrote the main manuscript text, and K.N. prepared figures. All authors reviewed the manuscript.
Funding
There is no funding received from any organization for this work.
Data availability
The datasets that have been used and analysed in this study are publicly available. PlantVillage crop disease data are on kaggle ( https://www.kaggle.com/datasets/abdallahalidev/plantvillage-dataset ) accessed March 2026). The dataset on the ripeness of apple fruits can be found in Kaggle ( https://www.kaggle.com/datasets/mdsagorahmed/fruit-image-dataset-22-classes ) accessed March 2026). The files used to run the implementation, trained model weights, class definitions and split metadata are opeOpen asset ↗Kaggle · plantvillage-datasetlines:583-665Dataset · publiction for this work.
Data availability
The datasets that have been used and analysed in this study are publicly available. PlantVillage crop disease data are on kaggle ( https://www.kaggle.com/datasets/abdallahalidev/plantvillage-dataset ) accessed March 2026). The dataset on the ripeness of apple fruits can be found in Kaggle ( https://www.kaggle.com/datasets/mdsagorahmed/fruit-image-dataset-22-classes ) accessed March 2026). The files used to run the implementation, trained model weights, class definitions and split metadata are openly available at: 10.5281/zenodo.19051520.
Declarations
Competing interests
The authors declare no competing interests.
References
1.
George R Thuseethan S RagelOpen asset ↗Kaggle · fruit-image-dataset-22-classeslines:583-665Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Accurate classification of corn leaf diseases is critical for timely detection and control of pests and diseases. By accurately recognizing different types of leaf diseases, farmers and agricultural experts can quickly take targeted control measures to reduce crop losses and safeguard corn yield and quality. Since these corn leaf disease images usually contain complex backgrounds, similar lesion features, and limited labeling data, it causes traditional convolutional neural networks (CNNs) to easily confuse the lesion region with the background, making it difficult to distinguish between different disease types. To address these limitations, we propose G-ResNet, a hybrid CNN-Vision Mamba network that enhances disease-relevant feature learning through a hierarchical feature attention module and a scale feature attention module. It was demonstrated experimentally that G-ResNet can better classify maize leaf disease images. The code is available at https://github.com/gustafmy/g_resnet.git.
Why it matches plant phenotyping methodsトウモロコシ葉の病害状態を画像から分類するCNN・Vision Mamba手法の開発と実験評価が研究の中心であり、植物病害フェノタイピングに該当する。
titleCorn or maize leaf disease classification based on CNN and vision mamba model.
Reproduction assets foundThe paper's analysis code (G-ResNet) is publicly available on GitHub with explicit availability statements, and the plant image dataset used for the disease classification experiments is a public Kaggle dataset explicitly cited in the Experiment section. The underlying data availability statement also mentions request,Code · publicSource code for the algorithms described in this paper is available at https://github.com/gustafmy/g_resnet.git.Open asset ↗gustafmy/g_resnethtml-lines:300-328Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Accurate identification of maize diseases is crucial for safeguarding global food security. Traditional image-based methods often struggle with lighting variations, occlusions, and noise, limiting their robustness and generalisation. Multimodal approaches that integrate visual and textual information have shown promise. However, these methods frequently require manually curated textual descriptions for each image, increasing data collection costs and limiting scalability and practical implementation. To address these limitations, we proposed a maize image-text framework with Cross-Modal Category Alignment (mIT-CMCA). This approach enforces category-level alignment between image and text modalities, enabling more accurate and interpretable cross-modal mapping. First, we construct cross-modal representations by aligning image and text modalities at the category level within a shared embedding space. Second, inspired by contrastive learning, we introduce a Cross-Modal Category Alignment (CMCA) loss based on category-level textual descriptions, reducing annotation complexity. Finally, we present an Efficient Channel-Spatial Hybrid Attention (CSHA) module that preserves inter-class boundaries while incurring minimal computational overhead, thereby enhancing feature discriminability under complex conditions. Experimental results on the maize subset of the PlantVillage dataset (MPVD) show that mIT-CMCA achieves 99.48% accuracy, 99.28% precision, 99.54% recall, and 99.41% F1-score. These results represent improvements of 0.24%, 0.13%, 0.17%, and 0.15% over the strongest vision-only baseline, MaxViT_tiny. On the self-built Maize Leaf-Field dataset (MLFD), the model achieves 93.67% accuracy, 93.76% precision, 93.67% recall, and 93.71% F1-score. It uses only 8.27 million parameters, which is 71.6% fewer than MaxViT_tiny. Its model size is 32.13 MB, which is 72.3% smaller. The proposed method also outperforms comparative models in robustness experiments under artificially added perturbations. These results demonstrate that mIT-CMCA achieves a favorable balance between accuracy and efficiency, making it suitable for practical agricultural deployment.
Why it matches plant phenotyping methodsトウモロコシ葉画像から病害状態を推定する画像・マルチモーダル手法の開発と性能評価が研究の中心であり、植物病害フェノタイピングに該当する。
abstractwe proposed a maize image-text framework with Cross-Modal Category Alignment (mIT-CMCA).
Reproduction assets foundThe paper's maize disease identification analysis code and trained models are explicitly stated as publicly available in a GitHub repository. The phenotype image datasets (MPVD subset and self-built MLFD) are not publicly available and require contacting the corresponding author.Code · publicCode availability
The code and models are available in the GitHub repository at https://github.com/TANGFEILONG626/mIT-CMCA..Open asset ↗TANGFEILONG626/mIT-CMCAhtml-lines:673-695Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The quality of forest reproductive material is crucial for successful reforestation and afforestation. While physical seed properties like mass are known indicators of quality, the potential of non-destructive, rapid color analysis for predicting germination in coniferous species requires further exploration. This study investigates the relationship between the seed coat color of individual Pinus sylvestris seeds, quantified in RGB (Red, Green, Blue) space using a flatbed scanner, and their subsequent germination in container nurseries. The resulting images were processed using ImageJ software to measure the mean pixel intensity (0–255) for the Red (R), Green (G), and Blue (B) channels from the segmented seed area, following the «seed–culture» passport methodology [Forestry Engineering Journal 14 | 55 (2024), 37–60]. From a population of individually tracked seeds, we compared the RGB values of germinated (N = 942) and non-germinated (N = 258) seeds after 30 days. Results from the Kolmogorov-Smirnov test showed that non-germinated seeds had significantly lower individual mass (p = 0.0045) and significantly higher pixel brightness values in the R-, G-, and B-channels (p < 0.0001) compared to germinated seeds. Normalized RGB indices also showed significant differences between groups. Our findings demonstrate that seeds with a lighter, more reflective epidermis – indicative of higher RGB brightness – are statistically associated with a lower probability of successful germination under container nursery conditions. This non-destructive, low-cost method shows significant promise for the rapid pre-sorting of Scots pine seeds. It offers a practical tool to improve the efficiency and predictability of seedling production in forest nurseries by increasing the proportion of viable seeds in sowing batches.
Why it matches plant phenotyping methods個別種子のRGB画像から種皮色を定量抽出し、発芽予測・事前選別に用いる非破壊的な表現型計測法が研究の中心である。
abstractthe potential of non-destructive, rapid color analysis for predicting germination in coniferous species requires further exploration
Reproduction assets foundThe paper openly deposits its three core phenotyping datasets in Mendeley Data: morphometric seed data (Dataset 1), the raw VIS/RGB scanner images of individual Pinus sylvestris seeds (Dataset 2), and germination outcome data (Dataset 3). All three DOIs are listed in the Data Availability statement and match allowed URDataset · publicThe original morphometric data—Dataset 1—of Pinus sylvestris L. are openly available in Mendeley Data at DOI: https://doi.org/10.17632/8g258nbgmf.1Open asset ↗Mendeley Data · 10.17632/8g258nbgmf.1lines:133-160Dataset · publicThe original VIS image data of Pinus sylvestris L. are openly available in Mendeley Data at DOI: https://doi.org/10.17632/dt78jhyw2j.2Open asset ↗Mendeley Data · 10.17632/dt78jhyw2j.2lines:133-160Dataset · publicThe original germination data—Dataset 3—are openly available in Mendeley Data at DOI : https://doi.org/10.17632/hrs3fgc8tt.1Open asset ↗Mendeley Data · 10.17632/hrs3fgc8tt.1lines:133-160Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Grain number estimation plays a crucial role in agriculture, serving as a key indicator for crop yield and quality assessment. With advances in computer vision, automatic grain detection has become a significant research area, where deep learning methods have shown remarkable promise. This study proposes a vision transformer model called Swin Transformer, which leverages hierarchical attention mechanisms across shifted windows to effectively capture both local and global features of grains in complex imagery. The model achieves the highest accuracy of 98%, outperforming baseline traditional CNN (ResNet-50) and DINO models in grain counting tasks. To support and validate model performance, explainable AI (XAI) techniques such as Grad-CAM and LIME are employed, highlighting the interpretability and focus of the model on relevant grain regions. Furthermore, a comprehensive empirical analysis is conducted using multiple statistical tests to evaluate the model's robustness and generalizability across various grain morphological parameters, establishing the Swin Transformer as a powerful and interpretable solution for intelligent grain counting in agricultural data analytics.
Why it matches plant phenotyping methods画像から穀粒数を推定する深層学習手法の開発・比較検証が研究の中心であり、植物の収量関連形質を測定するため、植物フェノタイピング手法として収録する。
abstractThis study proposes a vision transformer model called Swin Transformer
Reproduction assets foundThe paper's Data availability statement names a public Kaggle dataset of wheat grain counting images used for the study's grain counting experiments. No author analysis code or trained model checkpoints are disclosed.Dataset · publicThe dataset used and/or analyzed during the current study is publicly available at: https://kaggle.com/datasets/ociule/wheat-grain-counting-100-images.Open asset ↗kaggle · ociule/wheat-grain-counting-100-imageslines:1190-1253Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Wood apple (Feronia limonia L.) is an underutilized perennial fruit tree with substantial ecological, nutritional, and economic potential, yet its phenotypic diversity and trait organization remain poorly characterized. Here, we applied a mixed-scale multivariate framework to resolve phenotypic structure in 62 wood apple genotypes using 31 ordinal and categorical vegetative, leaf, floral, fruit, and seed descriptors. Trait interrelationships were examined through the complementary use of Spearman’s rank correlation and Cramér’s V association analyses, capturing both directional rank-based dependencies and scale-independent categorical linkages. Hierarchical clustering based on Gower distance separated the genotypes into three distinct phenotypic clusters, with inter-cluster dissimilarities (0.92–1.18) consistently exceeding intra-cluster variation (0.42–0.55), indicating well-supported phenotypic stratification based on cluster validation. Multiple Correspondence Analysis (MCA) explained 23.30% of total inertia across the first two dimensions, with tree growth habit, branch angle, tree shape, and fruit color emerging as the principal drivers of phenotypic differentiation. Vegetative and leaf traits formed a tightly integrated module, whereas fruit-related traits displayed weaker monotonic but persistent categorical associations, reflecting partial phenotypic independence. The strong concordance among association analyses, clustering, and MCA indicates structured patterns of coordinated and partially independent trait associations in wood apple. Overall, this study demonstrates the effectiveness of mixed-scale multivariate approaches for resolving complex trait architecture in underutilized perennial fruit crops and provides a quantitative phenotypic framework to support germplasm conservation, parent selection, and ideotype-oriented improvement of wood apple.
Why it matches plant phenotyping methods混合尺度の多変量解析を用いて植物遺伝資源の表現型構造を定量化する手法が研究の中心であり、単なる生物学的実験の routine 測定ではない。
abstractHere, we applied a mixed-scale multivariate framework to resolve phenotypic structure in 62 wood apple genotypes using 31 ordinal and categorical vegetative, leaf, floral, fruit, and seed descriptors.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Supplement · publicAll data generated or analyzed during this study are available in the article and the accompanying Supplementary Table S1.Open asset ↗lines:137-161Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Plant diseases are a major problem for farmers around the world, reducing crop yields. The absence of expertise makes plant disease detection difficult and complicated. Plant disease detection is made easier by deep learning algorithms; however, they are computationally demanding and need huge training datasets. This research work proposes a novel Conv-7 DCNN model with modified ParNet attention layer to classify plant leaves into distinct categories with improved accuracy. Because of its architecture, the proposed network can identify leaf diseases with more accuracy by extracting the wider range of features from the images. The proposed Conv-7 DCNN model classifies the leaf diseases of three plants such as tomato, potato, and pepper-bell into fifteen categories. The CNN model is trained using publicly accessible Kaggle dataset, utilising image augmentation techniques. It is evident from the simulation results that the proposed model outperforms several pre-trained, and other trending deep learning models. Proposed model achieves 99.18% classification accuracy with an average precision of 99.17% and area under the curve (AUC) of 1, making this model highly effective in leaf diseases detection. Additionally, Conv-7 DCNN achieved high FPS of 112.49, low inference time of 18.34 s, and low GFLOPS of 13.98, making it suitable for real-time applications in smart agriculture systems.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する深層学習モデルを開発・比較しており、病害表現型の取得・分類手法が研究の中心である。
abstractThis research work proposes a novel Conv-7 DCNN model with modified ParNet attention layer to classify plant leaves into distinct categories with improved accuracy.
Reproduction assets foundThe paper's plant-phenotyping measurements (leaf disease classification of tomato, potato, and pepper-bell) are based on a publicly available Kaggle dataset explicitly named in the Data Availability statement. No author analysis code, trained model checkpoints, or other paper-specific assets are disclosed.Dataset · publicThe dataset is available online at https://www.kaggle.com/datasets/emmarex/plantdisease.Open asset ↗Kaggle · emmarex/plantdiseasehtml-lines:824-854Code / dataset availability confirmedEurope PMC · Crossref · checked 5 Sept 2026
Accurate and efficient grain quality assessment is critical for making informed decisions throughout the grain value chain. Early detection of disease enables actions to mitigate spread and further damage, and optimal batch mixing to fulfill specified quality requirements allows for maximizing value and minimizing scrapping. Vision based machine learning and deep learning approaches are gaining attention in the agricultural sector and are useful for the development of automated grain quality assessment. These techniques can reduce the current manual inspection load and are key for objective and precise analysis. Yet, the majority of prior studies are constrained to small or controlled and curated datasets. Practical challenges associated with real-world deployment and reliability are rarely addressed. That is the focus of this work. We present and demonstrate a structured approach for investigating convolutional neural networks (CNNs) and key factors influencing performance for wheat kernel classification. The objective is to determine a CNN model that ensures high and robust classification accuracy, while elucidating and explaining how different image dataset characteristics and training parameters affect performance and reliability. We use a commercial mirror-based imaging system that captures over 90% of each kernel's surface and contrast and compare model architectures, robustness, the effect on pre-processing and image resolution. Our results show similar and high overall performance for ResNet50V2 and EfficientNetV2B0 ([Formula: see text]% accuracy), but per-class analysis indicate that the smaller classes suffer from lack of representative examples, and that most classes benefit from pre-processing including downsampling whereas others benefit from higher resolution. Interactive visualizations reveal that another contributing factor is dubious annotation and multi-class belongingness. Thus, our step-by-step analysis of CNN performance underscores the need for representative data, proper pre-processing, and class-aware evaluation to ensure trustworthy deployment in wheat grain quality assessment.
Why it matches plant phenotyping methods小麦粒画像から品質・病害クラスを推定するCNN画像解析手法の性能、頑健性、前処理、解像度、データ特性を体系的に評価しており、フェノタイピング手法が中心的である。
abstractWe present and demonstrate a structured approach for investigating convolutional neural networks (CNNs) and key factors influencing performance for wheat kernel classification.
Reproduction assets foundThe paper's wheat grain image dataset has a publicly available subset deposited on Zenodo (DOI 10.5281/zenodo.17397123), explicitly stated in the Data Availability statement. The full dataset is proprietary; code is only available upon request, so no qualifying code asset.Dataset · publicA publicly available subset of the segmented wheat grain images used in this study has been deposited in Zenodo to support transparency and reproducibility. The dataset includes representative samples per class collected from instrument and can be accessed at https://doi.org/10.5281/zenodo.17397123.Open asset ↗Zenodo · 10.5281/zenodo.17397123html-lines:337-368Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Climate change, particularly increasing frequency and intensity of spring frost events, poses a serious threat to viticulture by reducing yield and product quality. This study proposes an image processing and machine learning-based framework for early, rapid, and accurate segmentation of frost damage in vineyards using YOLOv11s enhanced with Atrous Spatial Pyramid Pooling (ASPP). A unique dataset called FGVL dataset from Sultana seedless grape vineyards in Manisa, Türkiye, following a severe frost event in April 2025. FGVL includes 418 frost-damaged grapes, 510 frost-damaged leaves, 395 healthy grapes, and 698 healthy leaves, all manually annotated by experts under natural field conditions. By integrating ASPP into YOLOv11s, proposed model improved multi-scale contextual feature extraction and achieved mAP@50 of 0.7686, demonstrating stronger performance in instance segmentation of small, overlapping, and visually similar grapevine organs. In addition, Dynamic Confidence Thresholding (DCT) strategy was introduced to improve prediction reliability in dense and visually complex vineyard scenes. Despite challenges such as background clutter, object overlap, and small target structures, model maintained stable performance with low computational demand, requiring only 6.45 GB of GPU memory. Proposed framework offers an accurate, efficient, and practically deployable early recognition system for frost damage assessment in viticulture.
Why it matches plant phenotyping methodsブドウの器官における霜害状態を画像からセグメンテーションする手法を開発・評価しており、植物の病害・障害状態の取得が研究の中心である。
abstractThis study proposes an image processing and machine learning-based framework for early, rapid, and accurate segmentation of frost damage in vineyards using YOLOv11s enhanced with Atrous Spatial Pyramid Pooling (ASPP).
Reproduction assets foundThe paper's Data availability statement explicitly shares the FGVL frost-damage dataset and source code in the corresponding author's public GitHub repository, matching an allowed URL.Code · publicSource code and dataset are publicly shared in GitHub repository of corresponding author. GitHub repo: https://github.com/kaanarikk/Grape-Instance-Segmentation-For-ViticultureOpen asset ↗https://github.com/kaanarikk/Grape-Instance-Segmentation-For-Viticulturelines:230-236Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Proper detection and treatment of plant leaf diseases are essential factors for achieving good crop yields and ensuring food security. Convolutional Neural Networks (CNNs) have shown significant potential for classifying diseases from leaf images. Instead, most current work focuses on image-level prediction and ignores the relationship between infected leaves. This limitation somewhat constrains their use in modelling disease spread. Also, it makes them less efficient in typical field situations where disease is transmitted from plant to plant by physical contact. Moreover, existing CNN architectures do not access inter-lobar contextual information, an essential factor for early detection and control. To tackle this, we propose AdjLeafGNN, an innovative hybrid deep learning and graph neural network model that performs multi-class leaf disease classification and probabilistic prediction of adjacent-leaf disease spread in a single pass. The method uses the enhanced CNN model (LDDNet), with Atrous Spatial Pyramid Pooling (ASPP) and a Channel-Spatial Attention Module (CSAM), to achieve a more precise representation across multiple scales. These embeddings are then used to construct a similarity graph, enabling a GNN to infer likely disease transmission paths among leaves. We evaluate the PlantVillage dataset on the proposed model, and the results show that it outperforms state-of-the-art CNN-based methods, achieving 98.88% classification accuracy and 98.71% F1 Score. Additionally, we were able to predict disease spread with a high AUC-ROC of 0.942 and an MCC of 0.884 using our framework. These results confirm that AdjLeafGNN can accurately model both local and relational patterns. The approach we propose is scalable and interpretable, facilitating real-time monitoring and control of diseases in precision agriculture.
Why it matches plant phenotyping methods葉画像から植物病害を分類し、隣接葉間の病害拡大を推定する深層学習・GNN手法を提案・評価しており、植物の病害状態の取得・推定が研究の中心である。
abstractwe propose AdjLeafGNN, an innovative hybrid deep learning and graph neural network model that performs multi-class leaf disease classification and probabilistic prediction of adjacent-leaf disease spread in a single pass.
Reproduction assets foundThe paper uses the public Kaggle PlantVillage leaf-image dataset as its phenotyping input and releases the complete AdjLeafGNN implementation (model, preprocessing, training, evaluation) on GitHub with a Zenodo-archived DOI.Dataset · publicthe dataset was obtained from the publicly available Kaggle distribution of the PlantVillage dataset: https://www.kaggle.com/datasets/mohitsingh1804/plantvillageTheOpen asset ↗html-lines:584-607Code · publicThe complete source code of the proposed AdjLeafGNN framework, including model implementation, training scripts, and evaluation pipeline, is publicly available. GitHub repository: https://github.com/surekhareddy123/AdjLeafGNN. A permanent archived version of the repository has been deposited in Zenodo and assigned the following DOI: 10.5281/zenodo.18862439.Open asset ↗https://github.com/surekhareddy123/AdjLeafGNN · 10.5281/zenodo.18862439html-lines:584-607Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Deep learning has improved automated plant disease detection by increasing recognition accuracy and robustness compared with traditional vision-based methods. Self-supervised learning (SSL) further reduces dependence on manual labels, but its transferability across heterogeneous agricultural datasets remains insufficiently characterized. Here, we evaluate a contrastive SSL pretraining and fine-tuning pipeline, termed PlantCLR, for plant disease classification under cross-dataset transfer with target-domain fine-tuning. PlantCLR combines SimCLR-style contrastive pretraining with a lightweight convolutional classifier to balance representation quality and deployment efficiency. Experiments on PlantVillage and Cassava Leaf Disease show strong performance, achieving 99.10% accuracy and 99.04% F1-score on PlantVillage, and 96.83% accuracy and 96.70% F1-score on Cassava. Feature embedding visualization using t-SNE and explanation maps using Grad-CAM indicate improved class separability and attention to disease-relevant regions. These results suggest that contrastive SSL can improve representation transfer while maintaining computational efficiency, supporting scalable plant disease diagnostics in practical agricultural settings. Code is available at GitHub .
Why it matches plant phenotyping methods植物病害を画像から分類するPlantCLR手法を開発し、異なるデータセット間で性能評価・検証しているため、植物の病害状態を推定するフェノタイピング手法が中心である。
abstractHere, we evaluate a contrastive SSL pretraining and fine-tuning pipeline, termed PlantCLR, for plant disease classification under cross-dataset transfer with target-domain fine-tuning.
Reproduction assets foundThe paper's plant disease detection experiments use two publicly available image datasets with explicit Kaggle URLs in the Data availability statement. The authors also state code is available at GitHub, but no concrete URL is provided, so no code asset is included.Dataset · publicThe Cassava Leaf Disease Classification dataset is available at https://www.kaggle.com/c/cassava-leaf-disease-classificationOpen asset ↗Kaggle · cassava-leaf-disease-classificationlines:232-268Dataset · publicthe PlantVillage dataset is available at https://www.kaggle.com/datasets/emmarex/plantdiseaseOpen asset ↗Kaggle · emmarex/plantdiseaselines:232-268Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Sugarcane diseases significantly reduce crop yield and quality, posing persistent challenges to the agricultural sector. This study presents a novel ensemble framework that integrates Vision Transformer and Swin Transformer architectures for accurate sugarcane leaf disease detection. By combining global self-attention with localized window-based attention mechanisms, the proposed model effectively captures multi-scale visual features associated with diverse disease symptoms. Experimental evaluation on a large, labeled sugarcane leaf dataset achieved a validation accuracy of 98.16% and a test accuracy of 97.06%, outperforming several convolutional neural network baselines. Additionally, a large language model (LLM) interface is employed as a post-prediction decision-support module, generating disease-specific descriptions and management suggestions based solely on the predicted disease class. This integrated framework indicates the potential effectiveness of transformer-based ensemble models combined with intelligent advisory support for practical decision-making in precision agriculture.
Why it matches plant phenotyping methodsサトウキビ葉の病徴を画像から分類するTransformerベースの手法開発と性能評価が中心であり、植物病害状態のフェノタイピングに該当する。
abstractThis study presents a novel ensemble framework that integrates Vision Transformer and Swin Transformer architectures for accurate sugarcane leaf disease detection.
Reproduction assets foundThe paper's sugarcane leaf disease image dataset (19,926 images, six classes) is explicitly stated to be publicly available on Kaggle; no author code or model checkpoints are shared.Dataset · publicThe Sugarcane Plant Diseases Dataset used in this study is publicly available on Kaggle at: https://www.kaggle.com/datasets/akilesh253/sugarcane-plant-diseases-dataset . The dataset is released for academic research and benchmarking purposes.Open asset ↗Kagglelines:112-131Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Abstract The precise classification of plant diseases is crucial for ensuring food security for all people and boosting agricultural productivity. Although there has been significant progress in this field using deep learning approaches, cross-dataset training hasn’t drawn as much attention from researchers as intra-dataset training has. Moreover, very few models have successfully blended intra-dataset and cross-dataset training approaches. This paper proposes a novel attention-based Convolutional Neural Network (CNN) to overcome these limitations. The model improves feature extraction and classification accuracy across multiple datasets by using attention mechanisms. It was tested on five datasets (Digipathos, Northern Leaf Blight (NLB), PlantVillage, PlantDoc, and the CD&S dataset) that covered leaf diseases of both corn and potatoes. During intra-dataset training, the model achieved the highest classification accuracy of 99.38% when trained on images of potato leaves from the PlantVillage dataset. During cross-dataset training, the model exhibited the highest average classification accuracy of 82.93% for corn leaf diseases when trained on images from the CD&S dataset with their backgrounds removed. When compared to the techniques taken into consideration in this study under comparable experimental conditions, the results demonstrate improved performance. This study shows how the model may be flexible for both intra- and cross-datasets, offering a flexible way to categorize diseases that affect plants. Because of its ability to generalize across different datasets, it may be helpful in real-world agricultural applications with a wide variety of image quality and situations. This encourages the advancement of precision farming techniques and disease control.
Why it matches plant phenotyping methods植物葉の画像から病害状態を分類するCNN手法の開発・データセット間検証が中心であり、植物病害の表現型推定に該当する。
abstractThis paper proposes a novel attention-based Convolutional Neural Network (CNN) to overcome these limitations.
Reproduction assets foundThe paper's plant disease classification experiments rely on five publicly available leaf-image datasets, each cited with an explicit public access URL in the reference list: PlantVillage (GitHub), PlantDoc (GitHub), Digipathos (Embrapa), NLB (SciDB), and CD&S (OSF). No author analysis code or trained model checkpoint,Dataset · publicHughes, D., & Salathé, M. (2015). An open access repository of images on plant health to enable the development of mobile disease diagnostics. arXiv preprint arXiv:1511.08060. Dataset accessed via GitHub: https://github.com/spMohanty/PlantVillage-DatasetOpen asset ↗GitHub · spMohanty/PlantVillage-Datasethtml-lines:1013-1082Dataset · publicDataset available at: https://github.com/pratikkayal/PlantDoc-DatasetOpen asset ↗GitHub · pratikkayal/PlantDoc-Datasethtml-lines:979-1012Dataset · publicCD&S dataset: Handheld imagery dataset acquired under field conditions for corn disease identification and severity estimation. arXiv preprint arXiv:2110.12084. Dataset available at: https://osf.io/s6ru5/files/osfstorageOpen asset ↗OSF · s6ru5html-lines:1013-1082Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
The growing global demand for food production, coupled with the increasing threat of plant diseases, necessitates advanced and automated solutions for crop health monitoring. Among various crops, pome fruits such as apples and pears are widely cultivated yet highly susceptible to multiple diseases that can significantly reduce yield and quality. Existing approaches for disease detection and severity classification are often limited by their dependency on manual inspection and their inability to handle complex real-world imagery, especially when multiple diseases coexist on a single leaf. To address these limitations, this research introduces a novel dual-model deep learning framework for multi-disease severity detection and classification in pome fruit leaves. A fine-tuned MobileNetV2 backbone is employed to extract high-level discriminative features from a specialized pome leaf dataset annotated with multiple disease types and severity levels. The proposed system integrates a lightweight Lite-U-Net for semantic segmentation to isolate diseased regions and an enhanced Lite-YOLACT for instance segmentation using a linear combination of prototype masks and mask coefficients. Moreover, a new multi-disease severity scale is proposed to quantify the impact of multiple coexisting infections on a single leaf, an aspect not addressed in previous studies. To enhance interpretability, an improved Grad-CAM technique generates visual heatmaps highlighting the most influential regions in the model's decision-making process, providing transparency and validation for agricultural experts. Experimental evaluations demonstrate that the proposed framework achieves 95% accuracy in disease severity estimation, effectively identifying and grading multiple infections simultaneously. This study represents a significant step forward in precision agriculture, offering an efficient, interpretable, and scalable deep learning solution for real-world crop health monitoring and management. The source code and trained models are publicly available at: https://github.com/mqasim0787/Multi-Disease-Severity .
Why it matches plant phenotyping methods果樹葉の病斑領域を画像から分割し、複数病害の重症度を定量推定する深層学習フレームワークが研究の中心であり、植物状態の画像ベース表現型計測に該当する。
abstractthis research introduces a novel dual-model deep learning framework for multi-disease severity detection and classification in pome fruit leaves.
Reproduction assets foundThe paper's authors publicly release source code and trained models on GitHub, and the study analyzes two public Kaggle plant-image datasets (DiaMOS Plant and PlantVillage) used directly for the multi-disease severity phenotyping experiments.Code · publicThe source code and trained models are publicly available at: https://github.com/mqasim0787/Multi-Disease-Severity .Open asset ↗https://github.com/mqasim0787/Multi-Disease-Severity · mqasim0787/Multi-Disease-Severitylines:1-23Dataset · publicThe datasets analyzed during the current study are available publicly in the Kaggle repository, DiaMOS dataset (1) and PlantVillage Dataset (2) 0.1. [https://www.kaggle.com/datasets/alexandraneagu101/diamos-plant-dataset]Open asset ↗https://www.kaggle.com/datasets/alexandraneagu101/diamos-plant-dataset · diamos-plant-datasetlines:964-977Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Early detection of maize leaf diseases is essential to prevent yield losses. Existing vision-based models face challenges in real-world environments due to data imbalance, lighting variations, and interpretability. This study presents MaizeFormerX, a lightweight Vision Transformer designed for cross-domain, explainable maize disease detection on resource-limited settings. MaizeFormerX employs multi-scale patch embeddings and a Cross-Scale Attention Fusion (CSAF) module to capture both detailed lesion textures and larger disease patterns. The CSAF output is processed through a transformer encoder stack using multi-head self-attention to model long-range dependencies. Robust preprocessing and dataset-specific augmentations were applied to improve feature extraction and address class imbalances in the Dataverse, Tanzania, and Plagues Maiz datasets. For interpretability, Grad-CAM was used for pixel-level saliency mapping in an efficient web application. When benchmarked against MobileViT, EfficientFormer, TinyViT, and Swin Transformer, MaizeFormerX achieved 97.8% accuracy on Dataverse, 97.5% on Tanzania, and 96.9% on Plagues Maiz, outperforming Swin Transformer V2 by 2–3%. Cross-domain testing yielded 88.9% accuracy when trained on Dataverse and tested on Tanzania, surpassing baseline performance by 3–6%. Class-wise analysis revealed F1 scores over 98% for Healthy and MLB classes with 6× augmentation, and over 97% for MSV. Ablation studies highlighted the significance of the cross-scale attention module for high MCC during domain shifts. This study introduces a precise, explainable, and efficient image-based method for classifying maize diseases, which could aid in more targeted crop management, reduce unnecessary agrochemical use, and promote sustainable maize production in future decision-support environments.
Why it matches plant phenotyping methodsトウモロコシ葉の病徴を画像から分類する手法の開発・ベンチマーク・交差ドメイン検証が中心であり、植物病害状態の画像ベース表現型計測に該当する。
abstractThis study presents MaizeFormerX, a lightweight Vision Transformer designed for cross-domain, explainable maize disease detection on resource-limited settings.
Reproduction assets foundThe paper's Data Availability statement explicitly lists three public maize leaf image datasets used for its phenotyping/disease-classification experiments (Dataverse, Tanzania/Mendeley, Plagues Maiz/figshare) and an authors' GitHub repository containing all code, preprocessing pipelines, and experimental configs. All四Dataset · publicThe datasets used in this study are publicly available and sourced from Dataverse (https://doi.org/10.7910/DVN/LPGHKK)Open asset ↗Dataverse · 10.7910/DVN/LPGHKKhtml-lines:2304-2339Code · publicAll code, preprocessing pipelines, and experimental configurations used in this work are available at: https://github.com/rezaul-h/MaizeFormerX/.Open asset ↗github · rezaul-h/MaizeFormerXhtml-lines:2304-2339Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Oranges, mandarins, bitter oranges, and lemons are examples of citrus fruits that make delicious meals and are highly nutritious. Citrus fruits suffer from a variety of infections that affect their yield. The Department of Agriculture wants to increase the production of oranges and lemons. On the other hand, several plant diseases and their advanced stages have impacted production. The quality of fruit influences market value and its financial effect. Therefore, accurate detection of ailments and their severity is crucial for improving the output and market value of oranges and lemons. To automatically evaluate and predict diseases in citrus leaves and fruits, this paper has proposed a modified convolutional neural network (ICNN) model. Python is used to create the ICNN model, and testing is performed using benchmark datasets from various repositories. The research presented here shows that ICNN performs better than traditional deep learning and machine learning models, such as the Convolutional Neural Network (CNN) and K-Nearest Neighbours (KNN). This illustrates how machine learning models require supplementary approaches to extract parameters from data that arrives in non-automated ways. Additionally, to improve the accuracy of their classification or prediction, deep learning models require pre-trained models. As a result, ICNN, an enhanced deep learning model that can automatically predict disease with higher accuracy than other models, represents an advancement over standard CNNs. Compared with KNN and CNN, ICNN achieves 99.69% accuracy.
Why it matches plant phenotyping methods柑橘の葉・果実の病害と重症度を画像から自動推定するCNN手法を開発し、ベンチマークデータセットで比較評価しており、植物フェノタイピング手法が中心である。
abstractTo automatically evaluate and predict diseases in citrus leaves and fruits, this paper has proposed a modified convolutional neural network (ICNN) model.
Reproduction assets foundThe paper's Data Availability statement explicitly lists three public Kaggle URLs as the datasets used and analysed in the study (citrus/plant leaf disease image datasets). These are paper-specific, publicly accessible image assets directly supporting the phenotyping/disease-classification analysis. The Mendeley URL (3Dataset · publicg agricultural specialists to properly understand and accept the model’s predictions.
Author contributions
Arunapriya.R – Problem Statements, Implementation and Testing Dr.S.P.Valli – Results, Conclusion, and Summary.
Data availability
The datasets used and/or analysed during the current study available and mentioned in below [ https://www.kaggle.com/code/ghazanfarali96/leaf-disease-classification-using-cnn-lstm-rnn ]. (https:/ www.kaggle.com/code/ghazanfarali96/leaf-disease-classification-using-cnn-lstm-rnn ). [ https://www.kaggle.com/code/moazeldsokyx/plant-leaf-diseases-detection-using-cnn ]. (https:/ www.kaggle.com/code/moazeldsokyx/plant-leaf-diseases-detection-using-cnn ). [ https://wwOpen asset ↗kagglelines:372-388Dataset · publicts, Conclusion, and Summary.
Data availability
The datasets used and/or analysed during the current study available and mentioned in below [ https://www.kaggle.com/code/ghazanfarali96/leaf-disease-classification-using-cnn-lstm-rnn ]. (https:/ www.kaggle.com/code/ghazanfarali96/leaf-disease-classification-using-cnn-lstm-rnn ). [ https://www.kaggle.com/code/moazeldsokyx/plant-leaf-diseases-detection-using-cnn ]. (https:/ www.kaggle.com/code/moazeldsokyx/plant-leaf-diseases-detection-using-cnn ). [ https://www.kaggle.com/code/ritzing/plant-disease-detection-using-keras-cnn-model ]. (https:/ www.kaggle.com/code/ritzing/plant-disease-detection-using-keras-cnn-model ).
Declarations
Competing interOpen asset ↗kagglelines:372-388Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Rice production is integral to the agricultural sector of India; over 65% of the populations are dependent on rice as their major staple. The cultivation of rice sustains this important agricultural sector; yet, there are many challenges encountered by rice producers, one of which is several types of disease that negatively impact yield and quality. Due to the fact that rice leaf smut, brown spot and bacterial leaf blights are among the most important types of diseases that can significantly reduce the yield and quality of rice, it is important to be diligent when identifying these diseases using accurate and speedy methods on an annual basis for successful and sustainable production of rice crops. As technology advances there continue to be emerging technologies such as Deep Learning (DL) as applied in agriculture to identify diseases and therefore reshape the agricultural paradigm so as to address agricultural disease challenges more readily. This research proposes a previously undemonstrated approach for identifying Rice Leaf Disease using EfficientNetV2; a Diffusion Bounded Attention method for disease detection. The quality of the input imagery has been greatly increased using a Preceding Noise Reduction (PNR) using the Guided Filopic Diffusion (GFD) technique, retaining important characteristics of Rice Leaves (Leaf Texture) which are critical for disease classification within agricultural imaging. To evaluate the performance of our model we utilized the Dice Similarity Coefficient (DSC). This coefficient measures how much the predicted image areas representing disease overlap with the actual affected areas of the image. Therefore, DSC is a reliable way to evaluate model segmentation capability. The Rice Leaf Diseases Dataset we used to identify and classify Rice Leaf Diseases was very comprehensive. Our model achieved an accuracy rate of 98.92% and also attained the best recall, precision and F1 score.
Why it matches plant phenotyping methodsイネ葉の病害症状を画像から検出・分類・セグメンテーションする手法が研究の中心であり、植物の病害状態を直接推定している。
abstractThis research proposes a previously undemonstrated approach for identifying Rice Leaf Disease using EfficientNetV2; a Diffusion Bounded Attention method for disease detection.
Reproduction assets foundThe paper's sole data asset is the public Kaggle Rice Leaf Diseases Dataset used for all experiments; no author code or models are deposited.Dataset · publicl analysis and data collection. N.K has done the initial drafting and statistical analysis. P.R. did the investigation. All the authors of the article have read and approved the final article.
Funding
Open access funding provided by Vellore Institute of Technology.
Data availability
The rice leaf disease data are assessed using https://www.kaggle.com/datasets/vbookshelf/rice-leaf-diseases
Declarations
Competing interests
The authors declare no competing interests.
References
1.
Upadhyay N Gupta N
Detecting fungi-affected multi-crop disease on heterogeneous region dataset using modified ResNeXt approach
Environ. Monit. Assess. 2024 196 7 610
10.1007/s10661-024-12790-0
38862723
Upadhyay, N. & Open asset ↗Kaggle · vbookshelf/rice-leaf-diseaseslines:553-627Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
To achieve agricultural automation, deep learning applications for early and accurate disease detection in tomato plants have been extensively developed. However, there is a fundamental trade-off between computational efficiency and diagnostic accuracy in resource-constrained agricultural edge environments. This paper proposes an evaluation framework for seven architectures that represent standard, efficient, and hybrid CNN structures to assess their implementation potential. Through evaluations of explainability, computational efficiency, and diagnostic performance, seven lightweight architectures (ShuffleNetV2, MobileNetV3-Small, SqueezeNet, MobilePlantViT, DenseNet121, ResNet50, and VGG16) are thoroughly examined. Three significant findings are derived from experiments conducted on a subset of tomato diseases in the PlantVillage dataset. First, the MobilePlantViT architecture accurately strikes the ideal balance between efficiency and performance. Second, in order to quantitatively assess the explainability of XAI models (Grad-CAM, SHAP, and LIME) and identify the best option for edge devices, we propose the perturbation stability score (PSS) metric. Third, we test CPU inference measurements to better reflect the actual scenario and find that the hybrid design effectively leverages parallel computing. According to these findings, MobilePlantViT is the ideal architecture for applications that require operation on edge devices with limited resources and achieve high diagnosis accuracy (above 99.5%).
Why it matches plant phenotyping methodsトマト病害という植物状態を画像から分類する深層学習手法について、複数モデルの精度・計算効率・説明可能性を体系的に比較評価しており、病害フェノタイピング手法の技術評価が中心である。
abstractThis paper proposes an evaluation framework for seven architectures that represent standard, efficient, and hybrid CNN structures to assess their implementation potential.
Reproduction assets foundThe authors publicly deposited their paper-specific tomato phenotype image subsets (derived from PlantVillage and expert-curated PlantDoc) on Kaggle via explicit Data Availability links. No author analysis code or trained model checkpoints are shared; ONNX Runtime is a generic library, not a paper-specific asset.Dataset · publicThe datasets are available at the following links: https://www.kaggle.com/datasets/cthngon/tomato-plantvillage-datasets, https://www.kaggle.com/datasets/cthngon/tomato-only.Open asset ↗Kaggle · cthngon/tomato-plantvillage-datasetshtml-lines:710-734Dataset · publicWe enhanced the quality of the PlantDoc dataset by collaborating with experts to identify and crop regions containing disease-specific symptoms, while eliminating irrelevant image content. For long-term preservation and ease of access, we have stored copies of the datasets in the published repository. The datasets are available at the following links: https://www.kaggle.com/datasets/cthngon/tomato-plantvillage-datasets, https://www.kaggle.com/datasets/cthngon/tomato-only.Open asset ↗Kaggle · cthngon/tomato-onlyhtml-lines:710-734Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
This paper presents a three-phase deep learning framework comprising (i) multi-modal data acquisition from drones and satellites, (ii) standardized pre-processing including interpolation for missing temporal data, and (iii) CNN-based feature extraction for real-time health classification. This framework relies on a mathematical model based on neural networks that classifies and detects the condition of agriculture, removing the reliance on manual tasks and subjective diagnosis. This paper focuses on three main aspects of our framework: data acquisition, training and prediction. Data is collected using sensors like drones, cameras, and satellite imagery and is pre-processed to filter out noise and improve quality. The training part uses CNN to learn features from the data and become more meaningful. The prediction part of the task classifies, and diagnoses crop health through the trained model using the features. The framework accuracy for crops such as maize, potato, and wheat has been tested and yielded over 90% accuracy. The novelty of this work resides in the development of a multi-modal deep learning architecture that fuses macro-scale satellite imagery with micro-scale drone and IoT sensor data to improve diagnostic reliability. The framework was validated on a multi-source agricultural dataset using a 70% training, 15% validation, and 15% testing protocol. Experimental results demonstrate an accuracy exceeding 90% for staple crops. Using this framework can increase the visibility and quality of information maintained for crop health and improve the decision-making routine of farmers in real time. Additionally, automation of this process can significantly reduce labor costs and increase productivity per crop. Implementing this framework can contribute to precision agriculture and sustainable management practices.
Why it matches plant phenotyping methods作物の健康状態を植物の表現型・状態として推定するマルチモーダル画像・センサ基盤と深層学習手法を開発し、複数作物・データセットで検証しているため、方法が中心である。
abstractThis paper presents a three-phase deep learning framework comprising (i) multi-modal data acquisition from drones and satellites, (ii) standardized pre-processing including interpolation for missing temporal data, and (iii) CNN-based feature extraction for real-time health classification.
Reproduction assets foundThe article's Data availability section points to a public Kaggle dataset used for the crop classification/health diagnosis experiments, matching an allowed URL. No code or model checkpoints are disclosed.Dataset · publicript. The research work was guided by Dr. B.D.K.P. The Corresponding author Shshank Chaube collaborated for review and supervision. All authors reviewed the manuscript.
Funding
Open access funding provided by Symbiosis International (Deemed University). No funds, grants, or other support was received.
Data availability
Dataset: https://www.kaggle.com/datasets/bhagvendersingh/precision-agriculture-dataset .
Declarations
Competing interests
The authors declare no competing interests.
Ethical approval
This article does not contain any studies with human participants or animals performed by any of the authors.
References
1. Mohyuddin, G. et al. Evaluation of machine learning approaches for preciOpen asset ↗kaggle · bhagvendersingh/precision-agriculture-datasetlines:473-545Code / dataset availability confirmedOpenAlex · Europe PMC · checked 5 Sept 2026
To address the inefficiency and high cost of manual counting of tobacco leaves, this study proposes a UAV-based method for automatic leaf counting in field-grown tobacco using 3D point clouds and an improved PointNext network. Although UAV imagery has been applied to crop phenotyping, most existing UAV-based leaf-counting methods still rely on 2D images or hand-crafted features and rarely exploit 3D point clouds with dedicated leaf-level segmentation, which limits accuracy and robustness under leaf overlap, variable viewing angles, and complex field backgrounds. In this work, oblique UAV photogrammetry is used to reconstruct individual plants into 3D point clouds, and a segmentation network, SRW-PointNext, is developed by integrating an SCSA attention mechanism and a Residual-SegHead to enhance feature extraction and segmentation performance, while a re-weighted loss alleviates class imbalance. Leaf point clouds are then clustered using MeanShift to obtain leaf counts. Experiments on field-grown tobacco demonstrate that the proposed method achieves a point-cloud segmentation precision of 92.09%, a MIoU of 76.13%. Compared with the original PointNext baseline, SRW-PointNext increased MIoU and overall precision by 3.34% and 2.42% respectively. The final accuracy rate of leaf counting was 92.61%, effectively achieving accurate and stable leaf counting under actual field conditions, and providing technical support for digital management, yield estimation and seedling breeding in tobacco production.
Why it matches plant phenotyping methodsUAV三次元画像と改良セグメンテーション手法により圃場タバコの葉数を推定する方法を開発・検証しており、表現型取得が研究の中心である。
abstractthis study proposes a UAV-based method for automatic leaf counting in field-grown tobacco using 3D point clouds and an improved PointNext
Reproduction assets foundThe paper reports a UAV-based tobacco leaf counting method with an annotated 1000-plant point cloud dataset and SRW-PointNext code, both explicitly declared publicly available at author-provided Zenodo and GitHub URLs matching the allowed list.Dataset · publicData supporting the reported results can be found at: https://zenodo.org/records/15130271 .Open asset ↗zenodo · 15130271lines:531-564Code · publicThe code used in this study is available at: https://github.com/Nan20377/SRW-Pointnext.git .Open asset ↗github · Nan20377/SRW-Pointnextlines:531-564Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Maize is a vital global crop, but its productivity is often threatened by plant diseases, highlighting the need for precise and timely diagnostic methods. Traditional manual inspection is inefficient and prone to errors, motivating the development of automated solutions. Recent advances in computer vision and deep learning have enabled effective automated plant disease diagnosis. While Convolutional Neural Networks (CNNs) and Vision Transformers (ViTs) have shown promise in plant disease classification, CNNs struggle to capture global contextual information, and ViTs require large datasets and high computational resources. Inspired by mixture-of-experts (MoE) architectures, we propose a lightweight hybrid model that integrates CNN and ViT components, adaptively emphasizing local or global features based on input characteristics. Evaluated on a novel, real-world dataset of full maize plant images, our approach achieves 99.90% classification accuracy, significantly outperforming state-of-the-art baselines such as MobileViT, PiT, EdgeNeXt, and DeiT. These results demonstrate that lightweight hybrid architectures can deliver high-performance disease diagnosis suitable for practical agricultural deployment. The code is available at: https://www.github.com/sabermehdipour/MXiT .
Why it matches plant phenotyping methodsトウモロコシ全身画像から病害状態を推定する軽量CNN-ViT手法を開発・評価しており、植物表現型取得・判定が中心的です。
abstractwe propose a lightweight hybrid model that integrates CNN and ViT components
Reproduction assets foundThe paper's authors' MXiT analysis code is publicly available via a GitHub URL stated in the abstract, and the PlantVillage image dataset used for evaluation is publicly available. The Plant Scanner maize dataset is paper-specific but only available upon request, so it is listed as request_only.Code · publicThe code is available at: https://www.github.com/sabermehdipour/MXiT.Open asset ↗sabermehdipour/MXiThtml-lines:1-77Dataset · publicThe PlantVillage dataset is publicly available (https://github.com/spMohanty/PlantVillage-Dataset).Open asset ↗spMohanty/PlantVillage-Datasethtml-lines:707-785Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Common beanLeafClassificationStress / disease detectionDisease symptoms / severity
Early identification of bean leaf diseases, particularly Angular Leaf Spot and Bean Rust, is vital for ensuring crop productivity and global food security, especially within smallholder farming systems where disease outbreaks can rapidly escalate and cause severe yield losses. Conventional disease identification through visual inspection is labor-intensive, subjective, and highly dependent on expert knowledge, making it impractical for large-scale agricultural monitoring. Although recent deep learning-based approaches have demonstrated impressive accuracy in plant disease classification, their inherent “black-box” nature significantly limits real-world adoption, as farmers and agronomists often lack the ability to understand, trust, or act upon unexplained predictions. To address these challenges, this study proposes an automated and explainable disease diagnostic framework based on a Vision Transformer (ViT-B/16) architecture optimized through transfer learning from ImageNet. Unlike traditional convolutional neural networks that primarily focus on localized features, the Vision Transformer processes images as a sequence of flattened patches and leverages self-attention mechanisms to capture long-range dependencies and global contextual patterns across the entire leaf surface. This global representation enables the model to detect subtle and spatially distributed disease symptoms that are often overlooked by CNN-based approaches. To further enhance transparency and interpretability, GradCAM + + is integrated into the framework as an explainable artificial intelligence (XAI) mechanism. This method generates class-specific heatmaps that visually highlight the exact pathological regions influencing the model’s predictions, thereby establishing a human-interpretable validation loop for farmers, agronomists, and domain experts. The proposed framework was evaluated on the publicly available I-Bean dataset, achieving a validation accuracy of 97.52% along with strong precision, recall, and F1-score performance. The generated GradCAM + + visualizations consistently demonstrate the model’s sensitivity to true diseased regions, reinforcing both the reliability and trustworthiness of its predictions. By combining high-capacity global feature learning with visual explainability, the proposed approach offers a scalable, transparent, and practical solution for real-world precision agriculture. This framework not only enhances diagnostic accuracy but also bridges the critical gap between model performance and user trust, enabling informed decision-making and timely disease management in modern farming environments.
Why it matches plant phenotyping methods画像から豆葉の病害症状を分類・可視化する手法が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として適格。
abstractThis method generates class-specific heatmaps that visually highlight the exact pathological regions influencing the model’s predictions
Reproduction assets foundThe paper uses the publicly available I-Bean bean leaf disease image dataset (Healthy, Angular Leaf Spot, Bean Rust) and points to it via a Data availability DOI (10.21227/4k7y-vs03), which is an allowed URL. No author analysis code or trained model checkpoint is explicitly deposited.Dataset · publicPSP and SNT: Problem Formulation and MethodologyAS and DS: Implementation and VisualizationMVV PK and KB: Original Draft and Supervision.
Funding
Open access funding provided by Symbiosis International (Deemed University). This research received no external funding.
Data availability
[https://dx.doi.org/10.21227/4k7y-vs03]
Declarations
Competing interests
The authors declare no competing interests.
The authors declare that they have no conflict of interest.
References
1.
Wang Y Wang Q Su Y Jing B Feng M
Detection of kidney bean leaf spot disease based on a hybrid deep learning model
Sci. Rep. 2025 15 1 11185
10.1038/s41598-025-93742-7
40169647
POpen asset ↗10.21227/4k7y-vs03lines:325-415Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Accurate and non-destructive assessment of fruit maturity is critical for sustainable agricultural practices. This study proposes a novel framework for evaluating strawberry ripeness using Mid-Infrared (MIR) spectroscopy combined with metaheuristic feature selection and supervised classification. A dataset of 443 strawberries spanning eight maturity stages was analyzed using six metaheuristic algorithms—Binary Grey Wolf Optimizer, Binary Particle Swarm Optimizer, Bee Colony Optimizer, Genetic Algorithm, Ant Colony Optimizer, and Gravitational Search Optimizer—integrated with four classifiers: Naïve Bayes, Decision Tree, Linear Discriminant Analysis, and Support Vector Machine. A new fitness function was designed to optimize classifier performance, and results were validated through Self-Organizing Map Neural Networks, cross-validation, and statistical significance testing. The Genetic Algorithm–Linear Discriminant Analysis combination achieved the highest and most stable accuracy (94.6–99%), outperforming existing image-based, deep learning, and conventional spectroscopic approaches while retaining interpretability. These findings demonstrate that metaheuristic-driven MIR analysis provides a robust, explainable, and efficient method for precise strawberry maturity assessment, offering significant potential for advancing eco-friendly and intelligent agricultural practices.
Why it matches plant phenotyping methodsイチゴ果実の成熟度という植物器官の状態を、MIR分光と特徴選択・分類器で非破壊推定する方法を開発し、交差検証や統計検定で性能評価しており、フェノタイピング手法が中心である。
abstractThis study proposes a novel framework for evaluating strawberry ripeness using Mid-Infrared (MIR) spectroscopy combined with metaheuristic feature selection and supervised classification.
Reproduction assets foundThe paper's analysis code is explicitly stated to be publicly available at the authors' GitHub release URL. The spectral dataset itself is not public and is available only from the corresponding author on request.Code · publicCode availability
The code is available publicly on:
https://github.com/RabihAssaf89/RabihAssaf-codes/releases/tag/v1.0.Open asset ↗RabihAssaf89/RabihAssaf-codes · v1.0html-lines:822-851Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Abstract Agriculture and global food security are critically dependent on accurate and timely identification of plant diseases and pests. Traditional approaches to disease identification rely heavily on visual inspection and expert knowledge, which frequently lack the accuracy, speed, and scalability needed to address growing agricultural challenges. Early and precise disease detection enables proactive interventions that can prevent widespread crop damage and reduce excessive pesticide use, thereby supporting sustainable agricultural practices. Artificial intelligence, particularly deep learning methods, has emerged as a transformative solution for automated plant disease diagnosis. Convolutional neural networks (CNNs) have demonstrated remarkable capabilities in image classification tasks, evolving from individual architectures to sophisticated ensembles and transferring learning models. However, existing CNN-based research on rice disease identification has typically focused on a limited number of disease classes, restricting their practical applicability in real-world agricultural settings. This study addresses these limitations by implementing DenseNet121, an advanced CNN architecture known for its efficient feature reuse and gradient flow, for comprehensive rice disease classification. We utilized a dataset comprising seven of the most common rice diseases, significantly expanding the scope beyond previous studies. The model employs transfer learning with pre-trained ImageNet weights and is optimized using the Adam optimizer with carefully tuned hyperparameters. The experimental evaluation on an independent test set demonstrates that our proposed model achieves an overall accuracy of 97.9%, with individual disease classification accuracy ranging from 94% to 99.67%. The model exhibits balanced performance across multiple metrics, including precision (96.2%), recall (97.97%), and F1-score (97%), confirming its robustness and generalizability. These results establish DenseNet121 as a highly effective framework for automated rice disease diagnosis, offering a practical tool for enhancing agricultural productivity and food security.
Why it matches plant phenotyping methodsイネ葉の画像から病害状態を分類する深層学習手法が研究の中心であり、独立テストセットによる性能評価も実施しているため、植物病害フェノタイピング手法として収載する。
abstractThis study addresses these limitations by implementing DenseNet121, an advanced CNN architecture known for its efficient feature reuse and gradient flow, for comprehensive rice disease classification.
Reproduction assets foundThe paper's rice disease classification experiments use the public Kaggle Paddy Disease Classification dataset (8030 images, 7 disease classes), explicitly cited and linked by the authors in the Data Availability Statement. No author code or trained model checkpoints are disclosed.Dataset · publicThe data presented in this study are available in Kaggle42.Open asset ↗Kagglehtml-lines:487-556Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Abstract Plant diseases continue to pose a significant threat to worldwide food security, resulting in notable yield reductions and economic consequences. Automated disease diagnosis through machine learning has arisen as a potential solution; nevertheless, current methods frequently have difficulty in capturing both detailed local attributes and overarching contextual patterns found in plant leaf images. This study presents a thorough comparative examination of conventional and deep learning methods—such as Convolutional Neural Networks (CNNs), Vision Transformers (ViTs), YOLO, Support Vector Machines (SVMs), and Random Forests—for the classification of multi-class plant diseases. To overcome the constraints of individual CNN and transformer models, a new hybrid framework that integrates EfficientNet-B7 for strong spatial feature extraction with a Vision Transformer (ViT-B16) for comprehensive contextual modeling is suggested. The system is assessed on an extensive dataset consisting of 21,534 images covering 38 classes of plant diseases and healthy specimens. Experimental findings show that the suggested hybrid model reaches an accuracy of 98.13%, surpassing standalone CNN baselines and other rival models, while consistently achieving high precision, recall, and F1-scores for all classes. The results emphasize the success of combining convolutional and transformer-based models for scalable and precise plant disease detection, aiding the creation of smart decision-support systems for precision farming.
Why it matches plant phenotyping methods植物葉画像から病害状態を分類する新規ハイブリッド画像解析手法を提案し、複数手法との比較評価と大規模データセットでの検証を行っており、フェノタイピング手法が中心である。
abstractAutomated disease diagnosis through machine learning has arisen as a potential solution
Reproduction assets foundThe paper's plant disease image dataset (New Plant Diseases Dataset on Kaggle) and the authors' complete hybrid CNN–ViT implementation (GitHub repository with Zenodo DOI) are both publicly and explicitly available.Dataset · publicThe data that support the findings of this study are openly available in the New Plant Diseases Dataset at Kaggle [https://www.kaggle.com/datasets/vipoooool/new-plant-diseases-dataset/data].Open asset ↗Kaggle · new-plant-diseases-datasethtml-lines:296-327Code · publicThe source code, including model architecture, training scripts, evaluation routines, and Google Colab notebooks for inference, is hosted on GitHub at: https://github.com/mohdzunaidahmed15-ui/hybrid-cnn-vit-plant-disease-diagnosis.Open asset ↗GitHub · mohdzunaidahmed15-ui/hybrid-cnn-vit-plant-disease-diagnosishtml-lines:296-327Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Potato leaf diseases, if left undetected, threaten food security in agricultural economies and cause substantial crop losses. To address this critical challenge, we developed an AI-based system called the Enhanced Single Shot Multibox Detector(EF-SSD), a variant of SSD that integrates multiscale feature fusion and Squeeze-and-Excitation attention to improve fine-grained lesion detection. The enhanced model processes high-resolution images (512×512 pixels) and analyzes leaves at ten magnification levels, enabling it to identify even minor signs of infection. The inclusion of Squeeze-and-Excitation filters allows the system to focus more effectively on characteristic disease patterns, increasing detection precision. After scanning the leaves, the system applies advanced image processing techniques to localize disease regions and assess their severity. We evaluated EF-SSD using 2,500 labeled potato leaf images representing healthy plants and cases of early and late blight. The proposed model achieved a mean Average Precision (mAP) of 97% at 0.5 IoU, an F1-score of 95%, and an Intersection over Union (IoU) of 89%, outperforming advanced detectors such as YOLOv5, YOLOv8, RetinaNet, and Faster R-CNN across all metrics. It also delivers real-time inference at 47 FPS, confirming its suitability for on-field deployment. An ablation study further demonstrates the effectiveness of SE blocks and extended feature hierarchies in enhancing detection accuracy. These outcomes highlight EF-SSD’s potential as a reliable, efficient, and scalable tool for smart agriculture and early crop disease management.
Why it matches plant phenotyping methodsジャガイモ葉の病斑を画像から検出・局在化し、病害の重症度を評価する深層学習手法を開発・検証しており、植物表現型取得が中心である。
abstractwe developed an AI-based system called the Enhanced Single Shot Multibox Detector(EF-SSD)
Reproduction assets foundThe paper's Data Availability statement explicitly provides a public GitHub repository with the authors' analysis/training code and a public Google Drive link to the custom 2,500-image potato leaf disease dataset with Pascal VOC annotations used in this study.Code · publicThe code implemented in this study is openly available at the following GitHub repository: https://github.com/bhavanisravan/potato-leaf-diseases-code.Open asset ↗bhavanisravan/potato-leaf-diseases-codehtml-lines:414-439Dataset · publicThe dataset used for training and evaluation can be accessed at: https://drive.google.com/drive/folders/1Yin9zp0gQKwqJ0LD3GWdLq_V_idLO2bG?usp=sharing.Open asset ↗html-lines:414-439Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Medicinal plants including Ocimum tenuiflorum L. (Tulsi), Azadirachta indica A. Juss. (Neem), and Kalanchoe pinnata (Lam.) Pers. (Patharkuchi) are essential sources of bioactive compounds, yet leaf diseases threaten their yield and phytochemical integrity. This study proposes LSeTNet, a lightweight hybrid CNN (Convolutional Neural Network) Transformer architecture with Squeeze-and-Excitation (SE) blocks, achieving 99.72% accuracy, 1.00 macro F1-score, and AUC = 1.00 across 12 disease classes (1,000 images/class post-augmentation) using only 9.38 M parameters and 2.50 GFLOPs. Five-fold cross-validation yielded 99.74% ± 0.14% accuracy, with rapid convergence and no overfitting. Explainable Artificial Intelligence (XAI) via Gradient-weighted Class Activation Mapping (Grad-CAM) (mean intensity: 0.1664-0.2702), Local Interpretable Model-agnostic Explanations (LIME), and t-distributed Stochastic Neighbor Embedding (t-SNE) (silhouette score: 0.87) confirmed biologically meaningful attention on pathological regions. External validation on the independent BD-MediLeaves dataset (8 classes, 8,000 samples) achieved 99.42% accuracy and 0.99 macro F1. With 6.98 ms/image inference latency and 35.81 MB memory, LSeTNet enables real-time, edge-based deployment. It significantly outperforms DenseNet169 (95.56%), ViT-B16 (95.61%), and LW-CNN+SE (95.39%) ([Formula: see text], paired t-tests), establishing a transparent, efficient, and generalizable benchmark for precision phytopathology and sustainable medicinal plant cultivation.
Why it matches plant phenotyping methods植物葉の病害状態を画像から分類するCNN・Transformer手法を開発し、交差検証、外部データセット、既存モデルとの比較で検証しており、植物フェノタイピング手法が中心である。
abstractThis study proposes LSeTNet, a lightweight hybrid CNN (Convolutional Neural Network) Transformer architecture with Squeeze-and-Excitation (SE) blocks
Reproduction assets foundThe paper publicly releases its primary medicinal leaf image dataset (MedicinalLeaf-12) on Mendeley Data, uses a public external validation dataset (BD-MediLeaves, also on Mendeley), and provides full training/evaluation code for LSeTNet on GitHub. All three are paper-specific, public, and actionable.Dataset · publicThe primary dataset used in this study is available in the Mendeley Data Repository: https://data.mendeley.com/datasets/ncg7kk3gwx/1 .Open asset ↗Mendeley Data · ncg7kk3gwx/1lines:712-750Code · publicThe full training and evaluation code for the proposed LSeTNet model is publicly available on GitHub at: https://github.com/mdtuhinkhan101/LSeTNet .Open asset ↗GitHub · mdtuhinkhan101/LSeTNetlines:712-750Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Field / plotLeafObject detectionStress / disease detectionDisease symptoms / severity
Timely localization and diagnosis of crop lesions are critical for disease control and reducing pesticide use. However, in-field lesions often resemble leaf textures, vary widely in scale, and suffer from lighting and shadow interference-making simultaneous high accuracy and lightweight inference challenging. We propose WGA-YOLO, a lightweight YOLO variant for crop disease recognition. Central to our design is Wavelet Channel Recalibration (WCR), a DWT-based downsampling module: discrete wavelet transform naturally provides multi-resolution, time-frequency localized representations that explicitly separate low-frequency approximations from high-frequency edge/texture details. WCR fuses high- and low-frequency components and enhances feature representation through their frequency-domain complementarity, thereby preserving semantic and fine texture information during resolution reduction with negligible extra cost. We also introduce PS-C2f, which integrates Pinwheel-shaped convolutions into C2f to better capture tiny lesion details via multi-directional, irregular kernels, and replace SPPF with Dynamic Group Attention Pooling (DGAP) for efficient multi-scale context aggregation. On our PlantDoc_boost dataset, WGA-YOLO improves over YOLOv8n by 3.02 and 2.85% points, while reducing parameters and FLOPs by ~ 0.18 M and ~ 0.3G, demonstrating improved inference efficiency and deployment friendliness while maintaining strong detection performance in field scenarios.
Why it matches plant phenotyping methods植物葉の病斑を画像から検出・診断するYOLO改良手法の開発が中心であり、病害状態の画像ベース表現型計測に該当する。
abstractWe propose WGA-YOLO, a lightweight YOLO variant for crop disease recognition.
Reproduction assets foundThe paper's PlantDoc_boost dataset (the annotated crop-disease image dataset constructed and analyzed in this study) is explicitly stated to be publicly released on the authors' GitHub repository. No author analysis code or trained model checkpoints are stated as available. The Roboflow corn and tomato datasets are preDataset · publicThe PlantDoc_boost dataset used and analyzed in this study is publicly available from the project repository at http://github.com/YongChaoLiang/PlantDoc_boost/tree/master.Open asset ↗YongChaoLiang/PlantDoc_boosthtml-lines:693-708Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Early and precise diagnosis of diseases in tomato plants is critical in ensuring productivity in agriculture and reducing losses caused by diseases. Classical classification approaches, however, are often limited by different image quality, different lighting, low resolution, and imbalanced classes. In order to confront these issues, this paper suggests a hybrid ensemble model that integrates the advantages of deep learning, fuzzy logic, and a generative model to classify diseases successfully. The suggested approach combines three strong convolutional neural networks, ResNet-50, EfficientNet-B0, and DenseNet-121, into the adaptive ensemble system. Individual models are combined to form the final decision depending on the predictive accuracy and confidence level. The use of fuzzy logic to refine intelligently the decision-making process provides more flexibility than the decisions of the static ensemble methods. A Conditional Generative Adversarial Network (C-GAN) is used to alleviate the problem of class imbalance and overfitting through the production of multiple synthetic images of high quality. This, in turn, significantly enhances the generalization of models and even gives a balanced representation of the disease’s classes. The hybrid structure achieved a classification accuracy of 99.19% when tested on the PlantVillage dataset and outperformed the traditional ensemble and classical techniques. The results highlight the potential of the hybrid approach for real-world agricultural applications. This offers a scalable, accurate, and intelligent solution for automated plant disease diagnosis. This study contributes a novel, interpretable, and performance-driven model that can support sustainable agriculture through timely and precise disease management in tomato crops.
Why it matches plant phenotyping methodsトマト葉画像から病害状態を分類する深層学習・ファジー論理・生成モデルの統合手法を開発し、PlantVillageで性能評価しているため、植物病害フェノタイピング手法が中心です。
abstractthis paper suggests a hybrid ensemble model that integrates the advantages of deep learning, fuzzy logic, and a generative model to classify diseases successfully.
Reproduction assets foundThe paper uses four public tomato leaf image datasets (PlantVillage, Tomato Leaves, PlantDoc, Tomato-Village) plus two Mendeley-hosted datasets for robustness evaluation, and declares a public GitHub repository of C-GAN-generated tomato crop images under Code availability. No trained model checkpoints or analysis code/Dataset · publicTomato Leaves Dataset
https://www.kaggle.com/datasets/ashishmotwani/tomato?
Over 20,000 samples of tomato leaves with 10 diseases and 1 healthy class are availableOpen asset ↗html-lines:399-523Dataset · publicThe “Tomato-Village” dataset: The “Tomato-Village” dataset is designed to improve tomato disease detection in real-world agricultural conditions. It is available at the following link.: https://github.com/mamta-joshi-gehlot/Tomato-VillageOpen asset ↗Tomato-Villagehtml-lines:1009-1039Dataset · publicMendeley Data: This dataset contains images of tomato leaves afflicted with distinct diseases gathered under several conditions. This is available on link: https://data.mendeley.com/datasets/zfv4jj7855/1Open asset ↗html-lines:1009-1039Dataset · publicGTLD: The images in the dataset were taken with a DSLR and a quality mobile phone. Some images were taken in direct sunlight, while others were captured in shaded areas beneath the plants. This is available on link: https://data.mendeley.com/datasets/2bdfjb99k5/1Open asset ↗html-lines:1009-1039Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 5 Sept 2026
Segmentation of hyperspectral image data is a well-established technique in remote sensing. While it is commonly applied to individual field crops, its use for individual trees is less prevalent. Conifers are crucial in forestry, and assessing physiological status, or genetic diversity is required for effective early-age treatment in nurseries and hyperspectral imaging (HSI) combined with high-throughput phenotyping (HTP) offers faster and non-destructive evaluation. NDVI-based thresholding is sufficient for detection of leaves with large projection areas, but needles of conifers present challenges due to spatial resolution constraints and increased proportion of border pixels. This study monitored the offspring of three locally adapted Scots pine (Pinus sylvestris L.) populations, representing distinct upland and lowland ecotypes. This study presents a hyperspectral image processing pipeline for segmenting and isolating individual Scots pine seedlings. Using a K-means algorithm, 23 hyperspectral centroids were successfully derived and subsequently classified into ten biologically distinct groups. Random forest classification model effectively differentiated Scots pine seedlings based on origin during water stress and recovery periods. This study highlights the potential of hyperspectral imaging and machine learning in evaluating the physiological state of conifer seedlings, demonstrating promising applications in forest tree physiology research and tree breeding.
Why it matches plant phenotyping methods個体のマツ苗を分離・セグメンテーションするハイパースペクトル画像処理パイプラインを開発し、機械学習で生理状態や由来を評価しており、表現型取得手法が中心である。
abstractThis study presents a hyperspectral image processing pipeline for segmenting and isolating individual Scots pine seedlings.
Reproduction assets foundThe paper's Data availability statement explicitly deposits demonstration hyperspectral sample data on Zenodo and the segmentation/classification scripts on GitHub; both are paper-specific, public, and actionable. Full experimental data is request-only and not listed as a public asset.Dataset · publicDemonstration sample data and their accompanying descriptions are available in the Zenodo repository (https://doi.org/10.5281/zenodo.17167809).Open asset ↗Zenodo · 10.5281/zenodo.17167809lines:161-192Code · publicThe scripts developed for this study are available on GitHub at: https://github.com/JCepl/Pine-hyperspectral-image-segmentaionCompleteOpen asset ↗GitHub · JCepl/Pine-hyperspectral-image-segmentaionCompletelines:161-192Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Hyperspectral reflectance provides rapid, non-destructive phenotyping of plant leaves. These data have been used to develop machine learning models for predicting diverse plant traits, yet key challenges remain. We collected hyperspectral reflectance data together with 25 anatomical, gas exchange, and chlorophyll fluorescence traits from 320 recombinant inbred lines grown over three seasons. Using these data, we systematically (1) compare the performance of PLSR and SVR across a wide range of traits, including also slow fluorescence kinetics, (2) assess model generalizability and transferability, and (3) investigate how different aggregation strategies affect predictive accuracy. Based on a nested cross-validation framework, single cross-validation with MSE as metric performed comparably to repeated cross-validation or PRESS-based calibration. Optimal performance of trait-specific predictions was found to be dependent on the combination of model and data aggregation levels. Structural and biochemical traits showed the best generalizability and transferability, whereas physiological traits, particularly those derived from gas exchange and fluorescence kinetics, exhibited markedly reduced transferability. Together, these results provide a rigorous benchmark for evaluating machine learning models for trait prediction from hyperspectral reflectance data, and highlight both the opportunities and limitations for achieving robust generalization across diverse environments and genotypes.
Why it matches plant phenotyping methodsハイパースペクトル反射データから植物形質を予測する機械学習手法を、複数形質・環境・遺伝子型で系統的に比較し、一般化性と転移性を厳密にベンチマークしているため、方法論が中心である。
abstractHyperspectral reflectance provides rapid, non-destructive phenotyping of plant leaves.
Reproduction assets foundThe paper's Data availability statement explicitly deposits all code and raw hyperspectral/trait data in a public GitHub repository, matching an allowed URL.Code · publicAll code and raw data to ensure reproducibility of the results can be accessed at: [https://github.com/Rudan-X/HyperspectralML](https:/github.com/Rudan-X/HyperspectralML).Open asset ↗Rudan-X/HyperspectralMLlines:158-246Code / dataset availability confirmedCrossref · Europe PMC · checked 5 Sept 2026
Abstract Plant diseases pose a critical threat to global food security, agricultural sustainability, and farmer livelihoods, particularly in regions with limited access to advanced diagnostic technologies. Traditional methods of disease detection rely heavily on manual inspection, which is time-consuming, error-prone, and often results in delayed interventions. This paper presents a novel, solar-powered autonomous robotic system designed to detect plant diseases in real time using deep learning and IoT technologies. The proposed system integrates a high-resolution imaging unit, IoT-based environmental sensors, and an onboard processing module based on Raspberry Pi. Deep CNNs, trained on diverse datasets including PlantVillage, are used for accurate disease classification, while soil moisture and temperature sensors provide contextual environmental data to support diagnosis. The robot’s mobility, powered by solar energy, allows for continuous field monitoring with minimal human intervention. Experimental results demonstrate the system’s high classification performance, achieving 99.39% training accuracy, 97.47% validation accuracy, and 97.13% testing accuracy. Furthermore, the model achieved 99.63% overall accuracy, with a Precision of 99.40%, a Recall/Sensitivity of 99.56%, an F1-score of 99.46%, and a Specificity of 99.99% across multiple disease classes. These results highlight the robustness of the proposed approach in real-world agricultural conditions, enabling reliable disease detection and monitoring. The integration of cloud-based monitoring enables farmers to receive real-time alerts and insights, supporting timely and informed decision-making. This cost-effective, scalable, and environmentally sustainable solution has the potential to transform precision agriculture by enhancing early disease detection, reducing pesticide overuse, and improving crop yield and health.
Why it matches plant phenotyping methods植物病害状態を画像と深層学習で検出するロボット型フェノタイピング基盤が研究の中心であり、技術性能も評価している。
abstractThis paper presents a novel, solar-powered autonomous robotic system designed to detect plant diseases in real time using deep learning and IoT technologies.
Reproduction assets foundThe paper's Data Availability statement explicitly points to the public Kaggle PlantVillage leaf-disease image dataset used to train the CNN models, which is a paper-specific, publicly accessible phenotyping image asset. No author code, models, or field-collected data are deposited.Dataset · publicThe data presented in this study are available in [kaggle and roboflow] at [ [https://www.kaggle.com/datasets/emmarex/plantdisease](https:/www.kaggle.com/datasets/emmarex/plantdisease) ], reference number [46].Open asset ↗kaggle · emmarex/plantdiseasehtml-lines:319-384Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Precise and timely identification of cotton leaf diseases is essential for sustaining crop yield and quality, yet manual inspection remains time-consuming, labor-intensive, and prone to error. Existing automated approaches are limited by insufficient dataset diversity, inconsistent evaluation practices, limited use of explainable AI (XAI), and high computational cost. To address these challenges, we propose an attention-enhanced CNN ensemble, namely CottonLeafNet, which integrates lightweight convolutional neural networks for accurate cotton leaf disease classification across two publicly available datasets. CottonLeafNet achieves state-of-the-art performance, obtaining 98.33% accuracy, a macro F1-score of 0.9833, Cohen's kappa of 0.9800, a mean PPV of 0.9838, and an NPV of 0.9967 on Dataset D1, with an inference time of 0.51 s per image. On Dataset D2, it reaches 99.43% accuracy, a macro F1-score of 0.9942, Cohen's kappa of 0.9924, a mean PPV of 0.9943, and an NPV of 0.9981, with a 0.40 s inference time. Moreover, a unified eight-class dataset created by merging both datasets yields a test accuracy of 99.08%. Robustness analysis under artificially induced class imbalance further confirms the model's stability, with consistently strong macro F1-scores. To evaluate the generalization capability of the proposed CottonLeafNet, we conducted cross-dataset experiments, and the results indicate that the model maintains moderate performance even when trained and tested on different datasets. Gradient-Weighted Class Activation Mapping (Grad-CAM) visualizations demonstrate that CottonLeafNet reliably attends to disease-relevant regions, enhancing interpretability. Finally, real-time feasibility is validated through a web-based deployment achieving ≈1 s inference per image. These results establish CottonLeafNet as an accurate, robust, interpretable, and computationally efficient solution for automated cotton leaf disease diagnosis.
Why it matches plant phenotyping methods綿花葉の画像から病害状態を推定する分類手法を開発・評価しており、植物病害フェノタイピングが中心的である。
abstractwe propose an attention-enhanced CNN ensemble, namely CottonLeafNet, which integrates lightweight convolutional neural networks for accurate cotton leaf disease classification across two publicly available datasets.
Reproduction assets foundThe paper's plant-phenotyping inputs are three publicly available Kaggle cotton leaf disease image datasets (D1, D2, and cross-dataset D3) explicitly named in the Data availability statement. No author analysis code, trained model checkpoints, or supplementary code repository is disclosed in the supplied blocks.Dataset · publicThe datasets analyzed during the current study are publicly available in the Kaggle repository. Dataset D1 can
be accessed atOpen asset ↗Kagglepdf-page:17 lines:68-84Dataset · publicThe dataset used for
cross-dataset testing is publicly available at:Open asset ↗pdf-page:17 lines:68-84Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Accurate localization of plant growth points is essential for precision agriculture applications, including electro-weeding and laser weeding. While crop and weed detection has been extensively studied, existing methods focus primarily on object-level recognition and often neglect fine-grained growth point localization. To address this limitation, we propose a novel training strategy, epoch-based prior annealing (EPA), which incorporates the excess green minus excess red (ExG-ExR) index as prior knowledge and introduces schedule factor and gain factor to effectively steer keypoint regression. The experimental results show that incorporating EPA improves keypoint localization performance, with mAP50 increasing by 0.024 and mAP50:95 by 0.011, while maintaining bounding box detection performance. The parameter sensitivity experiments confirmed that both excessively strong and weak guidance can hinder training. Furthermore, analysis of parameters and computational cost shows that the additional overhead introduced by the EPA strategy accounts for less than 0.5% of the total, and be considered negligible. In summary, the proposed EPA strategy significantly improves the accuracy, robustness, and generalizability of plant and growth point detection models, offering a practical and scalable solution for precision agricultural applications.
Why it matches plant phenotyping methods植物の生長点を画像から局在化する学習戦略を開発し、その性能を実験的に検証しており、植物器官の表現型取得が中心です。
abstractwe propose a novel training strategy, epoch-based prior annealing (EPA), which incorporates the excess green minus excess red (ExG-ExR) index as prior knowledge and introduces schedule factor and gain factor to effectively steer keypoint regression.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicYou can access the dataset used in this study at the following links: https://github.com/cropandweed/cropandweed-dataset.Open asset ↗cropandweed/cropandweed-datasethtml-lines:497-528Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Potato plants are highly vulnerable to numerous diseases that can substantially affect both yield and quality. Conventional approaches for detecting these diseases are often labor-intensive, slow, and prone to inaccuracies, particularly under variable environmental conditions. This study presents a hybrid deep learning architecture, termed potato leaf diseases DenseNet (PLDNet), which integrates a DenseNet-based convolutional neural network with a Transformer-based attention module to accurately classify potato leaf diseases. Furthermore, an adaptive parametric activation function, referred to as Adaptive Flatten p-Mish (AFpM), is proposed to enhance the model's learning flexibility and representational capacity. When evaluated on the PlantVillage and Mendeley datasets, PLDNet attains classification accuracies of 99.54% and 87.50%, respectively, surpassing contemporary state-of-the-art models and activation techniques. The proposed framework exhibits strong generalization performance and offers a scalable, efficient approach for automated plant disease identification. To highlight the novelty, the proposed AFpM activation function introduces a learnable parameter enabling adaptive nonlinearity, improving over Mish, Swish, and PFpM activation functions through dynamic gradient control. AFpM improves accuracy by 2.52% on Mendeley dataset, and 1.93% on PlantVillage dataset compared to PFpM, and by more than 3% compared to Swish and Mish.
Why it matches plant phenotyping methods葉画像から植物病害状態を推定する深層学習モデルと新規活性化関数を開発・評価しており、植物フェノタイピング手法が研究の中心である。
abstractThis study presents a hybrid deep learning architecture, termed potato leaf diseases DenseNet (PLDNet), which integrates a DenseNet-based convolutional neural network with a Transformer-based attention module to accurately classify potato leaf diseases.
Reproduction assets foundThe paper's phenotyping inputs are two public leaf-image datasets used directly for its classification experiments: the Mendeley Potato Leaf Disease dataset (explicitly deposited with URL) and the PlantVillage dataset via Kaggle (explicitly linked in Data availability). The authors' PLDNet code is only promised 'upon' Dataset · publicsis. A.M initially drafted the paper, and all the authors (A.M, A.C, and N.A) reviewed and edited the paper.
Funding
Open access funding provided by University of Inland Norway. INN have subscription for open-access (OA) publication in Scientific Reports, Nature.
Data availability
The dataset used in this study is available at: https://data.mendeley.com/datasets/ptz377bwb8/1 and https://www.kaggle.com/datasets/emmarex/plantdisease .
Code availabilityOpen asset ↗Mendeley · ptz377bwb8/1lines:1390-1403Dataset · publicthors (A.M, A.C, and N.A) reviewed and edited the paper.
Funding
Open access funding provided by University of Inland Norway. INN have subscription for open-access (OA) publication in Scientific Reports, Nature.
Data availability
The dataset used in this study is available at: https://data.mendeley.com/datasets/ptz377bwb8/1 and https://www.kaggle.com/datasets/emmarex/plantdisease .
Code availabilityOpen asset ↗Kaggle · emmarex/plantdiseaselines:1390-1403Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Timely crop stress detection is essential for safeguarding yields and promoting sustainable agriculture. Traditional vegetation indices (e.g., NDVI, EVI) are widely used but remain static, crop-agnostic, and often insensitive to early stress signals. This study proposed RL-VI, a reinforcement learning-based framework that dynamically formulates vegetation indices optimized for rice stress detection. Unlike existing methods, RL-VI integrates Sentinel-2 multispectral imagery with smartphone-captured RGB data, creating the first cross-platform environment where vegetation indices are learned rather than predefined. The reinforcement learning agent adaptively selects stress-sensitive spectral band combinations guided by classification rewards. Experiments on real-world rice fields in Tamil Nadu, India, and benchmark datasets (Indian Pines, wheat salt stress) show that RL-VI achieves an overall accuracy of 89.4% and F1-score of 0.88, outperforming static and machine-learned indices by up to 12%. Importantly, RL-VI enables early stress detection up to 10 14 days before visible symptoms, providing actionable lead time for intervention. The proposed framework is computationally lightweight and scalable to UAV or edge devices, offering a farmer-ready tool for precision agriculture, bridging field-level mobile sensing with satellite monitoring for low-cost, real-time crop health management. Statistical validation using ANOVA (F = 88.24, p < 0.001) and pairwise t-tests (p < 0.001) confirmed RL-VI's superiority, while SHAP analyses emphasized the physiological significance of red-edge and SWIR bands in stress discrimination.
Why it matches plant phenotyping methods植物ストレス状態を推定する動的植生指数と強化学習フレームワークを開発し、実圃場・ベンチマークデータで性能検証しているため、フェノタイピング手法が中心である。
abstractThis study proposed RL-VI, a reinforcement learning-based framework that dynamically formulates vegetation indices optimized for rice stress detection.
Reproduction assets foundThe paper publicly releases its authors' field-captured mobile RGB rice canopy dataset on Kaggle and its full RL-VI analysis code (RL formulation, preprocessing, VI computation, training, evaluation) on GitHub. Sentinel-2 imagery and benchmark datasets are third-party public sources, not paper-specific deposits.Dataset · publicThe Mobile RGB dataset, consisting of field-captured rice canopy images collected by the authors at Polur, Tamil Nadu, India, is publicly available on Kaggle under a CC BY-NC 4.0 license (DOI: [https://doi.org/10.34740/kaggle/dsv/14105754](https:/doi.org/10.34740/kaggle/dsv/14105754)).Open asset ↗Kaggle · 10.34740/kaggle/dsv/14105754html-lines:616-683Code · publicAll custom code developed for this work including the RL-VI (Reinforcement Learning–based Vegetation Index) formulation algorithm, image preprocessing scripts, vegetation index computation modules, model training pipelines, and evaluation routines is openly accessible in a public GitHub repository. The code is available without restriction for non-commercial research use and fully available at Github Repository (https://github.com/Poornisrm/Vegetation-Index.git).Open asset ↗GitHub · Poornisrm/Vegetation-Indexhtml-lines:684-711Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Agriculture is a cornerstone of the economies of many countries, and wheat is a staple cereal crop that sustains nearly half of the worldwide population. However, production of wheat is highly vulnerable to biotic stress such as pathogens and pests, as well as adverse environmental conditions. These factors significantly affect yield and quality, posing critical threats to food security and economic resilience. Conventional disease detection methods often involve intense human labor, prolonged procedures, and are predisposed to subjectivity. Therefore, the development of an automated, accurate, and real-time disease monitoring system is imperative for modern precision agriculture. We propose a hybrid deep learning based Multi-Disease Detection Framework for Wheat Diseases (MDDM-WD) for the identification of multiple wheat diseases using UAV imagery. The framework leverages the pre-trained VGG-16 convolutional neural network for deep feature extraction via a transfer learning approach. These features are subsequently classified using Support Vector Machine (SVM), Random Forest (RF), Decision Tree (DT), XGBoost, and Bernoulli Naïve Bayes (BNB) algorithms of machine learning. The model is trained and evaluated on a custom-curated dataset, containing wheat diseases: stripe rust, powdery mildew, scab (Fusarium head blight), and yellow dwarf. Evaluation of experiments demonstrates that the classification performance is enhanced significantly through our hybrid approach, with accuracy ranging from 74 to 97%, precision from 73 to 96%, and recall from 73 to 95.7%. The SVM-based variant of the model achieved the highest performance, yielding 96% precision, 95.7% recall, 96% F1-score, and 97% accuracy. The proposed two-phase fine-tuned system demonstrates its effectiveness and efficiency in detecting multiple wheat diseases. The MDDM-WD model offers a resource-efficient and scalable approach for early disease detection, supporting informed decision-making for farmers, agronomists, and policymakers in advancing sustainable agriculture.
Why it matches plant phenotyping methodsUAV画像からコムギの病徴・病害状態を直接推定する深層学習フレームワークを開発・評価しており、植物表現型取得法が中心です。
abstractWe propose a hybrid deep learning based Multi-Disease Detection Framework for Wheat Diseases (MDDM-WD) for the identification of multiple wheat diseases using UAV imagery.
Reproduction assets foundThe paper's wheat disease image analysis is based on two public datasets (DAE-Mask GitHub dataset and a Kaggle multi-class crop disease image dataset), explicitly declared as open-source and publicly available in the Data Availability statement. No author code or trained model is released.Dataset · publicThe two datasets analyzed during the current study are open-source and publicly available online in the repositories https://github.com/YcZhangSing/Dataset-of-DAE-Mask and https://www.kaggle.com/datasets/jawadali1045/20k-multi-class-crop-disease-images.Open asset ↗https://github.com/YcZhangSing/Dataset-of-DAE-Maskhtml-lines:699-732Dataset · publicThe two datasets analyzed during the current study are open-source and publicly available online in the repositories https://github.com/YcZhangSing/Dataset-of-DAE-Mask and https://www.kaggle.com/datasets/jawadali1045/20k-multi-class-crop-disease-images.Open asset ↗https://www.kaggle.com/datasets/jawadali1045/20k-multi-class-crop-disease-imageshtml-lines:699-732Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Effective pest and disease detection plays a crucial role in minimizing crop losses and improving decision-making in precision agriculture. Among the most destructive pests affecting maize crops globally is the Fall Army Worm (FAW), known for its rapid spread and high impact on yield. Existing detection practices often rely on manual scouting, which can be inefficient, labour intensive and prone to human error. This study proposes a novel deep learning based framework for the automatic classification of FAW infested and healthy maize crops by integrating RGB and thermal image modalities. The core objective is to enhance detection accuracy through multimodal image fusion. A hybrid DNN-ViT model is introduced, combining two complimentary pipelines: (i) feature-level fusion, where CNN extracted features from RGB and thermal images are fused and classified using a Deep Neural Network (DNN) and (ii) image-level fusion, where a 6 channel RGB-thermal image is directly processed using a modified Vision Transformer (ViT). Experimental results demonstrate that the fused model achieved superior performance with an accuracy of 0.98, precision, recall and F1-score of 0.98 and AUC-ROC of 0.98 on the test set, outperforming models trained on RGB-only, thermal-only and unfused data. The ablation study confirms the effectiveness of multimodal fusion, with the no-fusion model showing significantly lower performance (accuracy-0.60 and AUC-ROC-0.67). This work highlights the benefits of integrating complementary data sources for robust crop health monitoring. Future research will explore enhanced fusion strategies, environmental robustness and field level deployment to validate the model's practical applicability.
Why it matches plant phenotyping methodsRGB・熱画像融合によるFAW被害・健全状態の画像判定モデルを開発し、融合方式や性能を比較検証しているため、植物の健康状態を取得する方法が中心である。
abstractThis study proposes a novel deep learning based framework for the automatic classification of FAW infested and healthy maize crops by integrating RGB and thermal image modalities.
Reproduction assets foundThe paper's paired RGB/thermal maize FAW image dataset is publicly deposited on Figshare (part of a peer-reviewed data publication), and the authors' custom Python analysis code is released as a public supplementary file (Supplementary Code.zip) with explicit availability language. The Figshare URL matches an allowed, Dataset · publicThe dataset has been made publicly available in the Figshare Data repository as a part of a peer reviewed data publication54. Detailed information on data acquisition, sensor specifications, environmental conditions and annotation protocols is provided in the associated data article. The dataset can be accessed at: https://figshare.com/s/677d2384ba6e02db9230 (10.6084/m9.figshare.28388018).Open asset ↗Figshare · 10.6084/m9.figshare.28388018html-lines:324-345Code · publicThe custom python code developed for this study is available as supplementary file (“Supplementary Code.zip”) and includes all scripts necessary to reproduce the multimodal feature fusion, image-level fusion and ablation experiments described in the manuscript. The dataset used is publicly available on Figshare. All dependencies are listed within the code file. Readers can execute the python script to reproduce the reported results.Open asset ↗html-lines:324-345Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Timely and precise detection of diseases on plants is crucial for minimizing losses during crop production in order to sustain food supply demands worldwide. In this work, deep learning (DL) was used to develop an automatic disease identification system for the leaves of potato and mango plants using two publicly available datasets, the PlantVillage Potato Leaf Disease (2,152 images) dataset and the Kaggle Mango Leaf Disease dataset (4,000 images). Images were pre-processed, augmented, and split into training and testing datasets (80:20), to enable better model generalization. Four deep learning architectures, namely Convolutional Neural Networks (CNN), AlexNet, Residual Networks (ResNet), and EfficientNet, were evaluated in the context of multi-class disease classification. The baseline CNN achieved a training accuracy of 93.67% and a testing accuracy of 92.61%, with balanced precision and recall (92.5%), thus providing a very strong feature extraction and classification capability. AlexNet showed moderate performance (91.3% training, 90.2% validation), and a very small overfitting was observed. ResNet had an efficient convergence, and attained 96.7% validation accuracy in just a few epochs, thus pointing out the advantage of residual connections in the context of deeper learning. EfficientNet surpassed all the other architectures, since it reached a training accuracy of 98.2% and a validation accuracy of 97.8%, with very small loss (≈ 0.015) and no overfitting, thus proving to have the best generalization ability. The models demonstrated stability and discriminative ability with the support of confusion matrices and accuracy and loss plots produced on an epoch-wise basis. Therefore, the findings indicate that DL models can be adapted for real-time and accurate plant disease diagnosis, establishing a pathway for early remediation, and supporting precision agriculture. The research establishes the opportunity for EfficientNet to be considered a promising solution for scalable smart farming.
Why it matches plant phenotyping methods葉画像から植物病害を分類する深層学習手法を開発・比較し、複数データセットで精度を検証しており、植物の病害状態の取得・推定が研究の中心である。
abstractdeep learning (DL) was used to develop an automatic disease identification system for the leaves of potato and mango plants
Reproduction assets foundThe paper uses two public Kaggle leaf-image datasets (PlantVillage potato, mango leaf disease) and states that all code, preprocessing scripts, dataset splits, and model artifacts are publicly available in a GitHub repository (also archived on Zenodo). All three are paper-specific, public, and actionable.Dataset · publicThe datasets analyzed during the current study are available in (https://www.kaggle.com/datasets/aarishasifkhan/plantvillage-potato-disease-dataset)Open asset ↗html-lines:473-503Code · publicAll code, preprocessing scripts, dataset splits, and model artifacts used in this study are publicly available in the GitHub repository at: [https://github.com/logeswarig/PROJECT_1].Open asset ↗logeswarig/PROJECT_1html-lines:473-503Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
Rice leaf diseases pose a significant and escalating threat to global food security. Timely and accurate detection, particularly in the critical early stages characterized by subtle lesions, is paramount for effective disease management. However, existing solutions often struggle with the complexities of real-world field environments (e.g., variable lighting, occlusions, complex backgrounds), computational constraints on edge devices, and limited generalizability across diverse disease types and plant species. To address these challenges, this study proposes a novel lightweight deep learning framework specifically designed for robust rice leaf disease detection. Our key innovations include: (1) A Multi-branch Large-kernel Fusion Depthwise (MLFD) module enhancing multi-scale contextual feature extraction critical for identifying subtle early lesions; (2) A Multi-scale Dilated Transformer Attention (MDTA) module integrating spatial and channel attention mechanisms to improve feature representation under complex conditions; (3) A Lightweight Detection Head (Lo-Head) optimized with grouped and depthwise convolutions, drastically reducing model complexity without sacrificing accuracy. Crucially, extensive experiments demonstrate the framework's superior performance. On a dedicated rice leaf disease dataset, it achieves a mean Average Precision mAP@0.5:0.95 of 62.62%, outperforming state-of-the-art lightweight detectors including YOLOv5n (56.73%), YOLOv8n (57.41%), YOLOv10n (56.14%), and the baseline YOLOv11n (60.85%), while maintaining low computational demands (6.3 GFLOPs, 2.66M parameters). Significantly, rigorous generalization experiments validate the model's exceptional transferability. Evaluated on independent datasets encompassing potato and tomato leaf diseases, the proposed framework consistently surpasses comparable models in mAP@0.5:0.95, demonstrating its robust capability to detect diseases across different plant species. This combination of high accuracy, computational efficiency, and remarkable cross-crop generalizability positions our framework as a highly promising tool for practical deployment on resource-limited edge devices (e.g., drones, field sensors) in smart agriculture systems, enabling proactive disease surveillance and precision control strategies across diverse crops.
Why it matches plant phenotyping methods植物葉の病徴を画像から検出する深層学習手法の開発と、独立データセットによる性能・汎化性検証が中心であり、植物の病害状態を直接推定するフェノタイピング手法に該当する。
abstractthis study proposes a novel lightweight deep learning framework specifically designed for robust rice leaf disease detection.
Reproduction assets foundThe paper's rice leaf disease detection dataset was curated from three publicly available repositories (one Kaggle, two Roboflow), and the cross-species generalization used two additional public Roboflow datasets (tomato and potato leaf diseases). All five URLs are explicitly listed in the article as data sources. No作者Dataset · publicData Sources: The dataset utilized in this study was curated and screened from the following publicly available online repositories:.Open asset ↗html-lines:110-216Dataset · publicThe Tomato Leaf and Potato Leaf disease datasets were acquired from public domain resources. The dataset links are: Tomato Leaf Diseases: https://universe.roboflow.com/dyploma/tomato-leaf-diseases-4xa5iOpen asset ↗dyploma/tomato-leaf-diseases-4xa5ihtml-lines:747-783Code / dataset availability confirmedEurope PMC · checked 5 Sept 2026
TomatoGreenhouseMultimodalRGB / grayscaleMultispectral / hyperspectralFruitClassificationSegmentationGrowth / development / phenology
Computer vision and multispectral imaging have increasingly become essential tools in modern precision agriculture. Accurate ripeness assessment is critical for yield optimization, reducing post-harvest losses, and enabling automated harvesting systems. However, traditional RGB-based approaches struggle to differentiate subtle maturity changes, and existing solutions often fail under varying lighting, occlusion, or cultivar-specific conditions. To address these challenges, this study focuses on the integration of complementary spectral cues for reliable tomato ripeness evaluation. The work utilizes a curated RGB-NIR tomato dataset comprising 224 hyperspectral samples, processed into aligned multimodal image pairs with balanced ripeness categories.The proposed TomatoRipen-MMT model employs a multimodal Transformer framework with dual encoders, cross-spectral attention, and a joint decoder to fuse spatial and biochemical cues. The novelty of the methodology lies in the dynamic cross-attention mechanism, which learns inter-modal dependencies between RGB and NIR signals for enhanced ripeness interpretation. Performance metrics including accuracy, precision, recall, F1-score, mIoU, and AUC were used to comprehensively evaluate the system. Experimental results demonstrate that TomatoRipen-MMT significantly outperforms all baseline RGB-only, NIR-only, and fusion methods, achieving 94.8% classification accuracy and 82.6% mIoU. These findings establish the effectiveness of multimodal Transformers for robust, high-precision fruit maturity assessment in controlled and greenhouse environments.
Why it matches plant phenotyping methodsトマト果実の成熟度という植物器官の状態を、RGB・NIR画像融合とTransformerで推定する手法を開発・評価しており、フェノタイピング手法が中心です。
abstractThe proposed TomatoRipen-MMT model employs a multimodal Transformer framework with dual encoders, cross-spectral attention, and a joint decoder to fuse spatial and biochemical cues.
Reproduction assets foundThe paper's phenotyping analysis is built on a publicly available USDA/NAL hyperspectral tomato dataset, explicitly linked in the Data Availability statement with an exact URL match. No author code or model checkpoints are disclosed.Dataset · publicThe dataset analyzed in this study is publicly available at the https://agdatacommons.nal.usda.gov/articles/dataset/Data_from_b_Hyperspectral_Imaging_Analysis_for_Early_Detection_of_Tomato_Bacterial_Leaf_Spot_Disease_b_/26046328.Open asset ↗26046328html-lines:1038-1053Code / dataset availability confirmedCrossref · Europe PMC · checked 6 Sept 2026
Abstract The development of accurate and efficient plant disease classification systems is vital for addressing the challenges of climate change and the growing global demand for food. This study presents $$\hbox {V}^2$$ PlantNet, a novel lightweight multi-class classification model based on a modified MobileNet architecture, designed to detect plant leaf diseases across a diverse range of crop types. $$\hbox {V}^2$$ PlantNet employs depthwise separable convolutions to significantly reduce model complexity without compromising accuracy. The architecture integrates Batch Normalization (BN) and Rectified Linear Unit (ReLU) activation after each convolutional layer, while a multi-stage design enhances feature extraction and overall performance. Despite its compact size, comprising only 389,286 parameters and requiring just 1.46 MB of memory, $$\hbox {V}^2$$ PlantNet achieved up to 99% training accuracy, with validation and test accuracies of 97% and 98%, respectively. Across most classes, precision, recall, and F1-scores ranged from 0.97 to 1.0, demonstrating consistent and robust generalization across diverse plant species. These architectural innovations enable $$\hbox {V}^2$$ PlantNet to outperform larger models such as ResNet-50 and Inception V3 in terms of computational efficiency, owing to its smaller model size (1.46 MB), reduced parameter count (389,286), and faster inference time (0.676 s), offering a scalable solution for real-time plant disease detection in precision agriculture.
Why it matches plant phenotyping methods植物葉の病害状態を画像から分類する軽量な深層学習手法を開発し、精度・計算量・推論速度を比較評価しており、フェノタイピング手法が中心である。
abstractThis study presents $$\hbox {V}^2$$ PlantNet, a novel lightweight multi-class classification model based on a modified MobileNet architecture, designed to detect plant leaf diseases across a diverse range of crop types.
Reproduction assets foundThe paper's Data Availability statement explicitly links the PlantVillage dataset used to train and evaluate PlantNet, hosted publicly on Kaggle. No author code or trained model checkpoint is deposited.Dataset · publicThe datasets generated and analysed during the current study are available in the Kaggle repository (see link to dataset: https://www.kaggle.com/datasets/abdallahalidev/plantvillage-dataset).Open asset ↗Kaggle · plantvillage-datasethtml-lines:1649-1677Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Cotton has, in recent years, become one of the most important cash crops worldwide while being impacted in yield from leaf disease which generally goes unnoticed in the early stage. Detection methods depend on manual efforts producing slow processes and human errors. Automated detection methods establish low accuracies, limited scalability and real time applications. To tackle the research issue, this study proposes the CLD-Net which stands for Cotton Leaf Disease Detection Network a novel deep learning-based framework which combines Faster-RCNN and YOLOv5 algorithms into a single action to achieve ultimately real time detection of accurate diseases the combination helps identify both the high detection speed of YOLOv5 along with Faster-RCNN regional proposal accuracy. The new method is that the compilation of these two modern object detection methods has been compiled and designed specifically for detecting leaf disease across varying environmental conditions. Notable contributions to this method include increases in classification accuracy, processing speed, real time detection making these methods suitable for farmers agronomists and sensor deployment. CLD-Net integrates YOLOv5 and Faster R-CNN, combining real-time detection capability with precise classification, to deliver robust cotton leaf disease identification. Experimental validation on a curated dataset of cotton leaf images demonstrates the superiority of CLD-Net, achieving an accuracy of 96.7%, which surpasses that of traditional models. These results confirm the potential of the proposed approach to revolutionize crop disease detection, leading to timely intervention and increased yield.
Why it matches plant phenotyping methods綿花葉の病害状態を画像から推定する深層学習手法を開発し、画像データセットで性能検証しており、植物フェノタイピング手法が中心です。
abstractthis study proposes the CLD-Net which stands for Cotton Leaf Disease Detection Network a novel deep learning-based framework which combines Faster-RCNN and YOLOv5 algorithms
Reproduction assets foundThe paper's Data Availability statement points to a public Kaggle cotton leaf disease image dataset used for the CLD-Net experiments; no author code or trained model deposit with a public URL is provided despite a mention of 'reproducible code and trained models'.Dataset · publicThe data used in this research are available in the following links: https://www.kaggle.com/datasets/seroshkarim/cotton-leaf-disease-dataset.Open asset ↗Kaggle · seroshkarim/cotton-leaf-disease-datasethtml-lines:541-573Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Early and accurate detection of sugarcane leaf diseases is critical for improving crop productivity and reducing economic losses in the agricultural sector. Timely interventions enable sustainable crop management and better resource use. In this study, we propose a deep learning-based approach for sugarcane leaf disease classification that leverages a novel architecture, the Multi-scale Attention-based Dense Residual Network (MADRN). The MADRN model integrates dense residual learning and multi-scale attention mechanisms to effectively capture fine-grained, disease-specific features and address challenges related to domain variability and complex data patterns. Two datasets are used to evaluate the model: a Kaggle dataset and a blended dataset created by combining Kaggle images with those from the Bangladesh Sugarcrop Research Institute (BSRI), simulating real-world conditions. All images undergo preprocessing steps, including resizing, normalization, and data augmentation, before training. Additionally, several baseline models (CNN, VGG16, MobileNetV2, and XceptionNet) are fine-tuned and compared with the MADRN model. Experimental results demonstrate that MADRN consistently outperforms baseline models in accuracy, precision, recall, and F1-score across both datasets, achieving up to 94.78% accuracy on the Kaggle dataset and 92.25% on the blended dataset. These findings highlight MADRN's superior ability to learn discriminative features and generalize effectively across diverse data sources, making it a promising tool for precision agriculture and disease management. To facilitate practical implementation, a web-based application is developed, enabling real-time and user-friendly disease detection. This research lays a strong foundation for the development of accurate, scalable, and practical disease classification tools that can support sustainable agricultural practices.
Why it matches plant phenotyping methodsサトウキビ葉の画像から病害状態を推定する深層学習手法を開発し、複数データセットとベースラインで比較検証しているため、植物フェノタイピング手法が中心です。
abstractwe propose a deep learning-based approach for sugarcane leaf disease classification that leverages a novel architecture, the Multi-scale Attention-based Dense Residual Network (MADRN).
Reproduction assets foundThe paper's Kaggle sugarcane leaf disease image dataset (2521 images, five classes) is a public, paper-specific phenotyping image asset with an explicit URL in the Data Availability statement. The BSRI field images are only available upon request, and no author analysis code or trained model is deposited.Dataset · publicg and preparation.
Funding
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Data availability
The data supporting the findings of this study are publicly available and can be accessed through the following sources. Kaggle Sugarcane Leaf Disease Dataset [https://www.kaggle.com/datasets/nirmalsankalana/sugarcane-leaf-disease-dataset](https:/www.kaggle.com/datasets/nirmalsankalana/sugarcane-leaf-disease-dataset) (accessed Jan. 15, 2025). Bangladesh Sugarcrop Research Institute (BSRI): Data available upon request. For BSRI data inquiries, please contact the corresponding author, Dr. Md. Shamim Reza.
Declarations
CompetinOpen asset ↗Kagglelines:278-299Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Recent advancements in precision agriculture have introduced innovative approaches to addressing plant stress, a critical factor influencing crop productivity and agricultural sustainability. Accurate, real-time prediction of plant stress has become essential for optimizing water utilization and promoting healthy crop development. While existing machine learning methods have demonstrated efficacy, they often lack the adaptability required to accommodate the dynamic conditions of agricultural environments. Prior research has identified soil moisture and chlorophyll content as key indicators of plant health and stress, with conventional models relying on simplistic algorithms for stress prediction. However, these models exhibit limitations in scalability, adaptability and interpretability. To overcome these challenges, this study employed sparse additive models with learning (SAM-L) algorithms, integrated with explainable artificial intelligence (XAI), to provide a flexible and transparent solution. In this paper, we proposed a novel framework that integrates SAM-L and XAI to predict plant stress using soil moisture and chlorophyll content. The SAM-L algorithm is a machine learning method that focuses on sparsely selecting relevant features through additive models. It aims to enhance model interpretability while maintaining high prediction accuracy by learning sparse representations of input data. The SAM-L algorithm enhances interpretability while preserving high predictive accuracy by learning sparse feature representations from input data. Additionally, XAI was incorporated to ensure interpretable decision-making, enabling farmers and stakeholders to comprehend the rationale behind irrigation recommendations. The model's architecture incorporates a three-layer Long Short-Term Memory (LSTM) network to process sequential data effectively. The proposed framework achieved a high performance on publicly available dataset, yielding an overall accuracy of 89.2% on the multi-class classification task. Further analysis of the results across the three predefined stress categories (healthy, moderate stress, and high stress) revealed strong performance, with the model obtaining a macro F1-score of 0.88 and a macro recall of 0.88. The proposed framework not only can enhance prediction accuracy but also can promote sustainable farming practices by reducing water wastage and improving crop resilience.
Why it matches plant phenotyping methods植物ストレス状態を土壌水分とクロロフィルから推定するSAM-L・XAI・LSTM統合手法が研究の中心であり、植物の生理状態を対象とした計算的フェノタイピング手法に該当する。
abstractIn this paper, we proposed a novel framework that integrates SAM-L and XAI to predict plant stress using soil moisture and chlorophyll content.
Reproduction assets foundThe paper states its plant-stress phenotyping data came from a publicly available Kaggle dataset ('Real-Time Plant Health Insights: Simulated Biosensor Data for AI-Driven Monitoring'), used directly for the SAM-L/XAI stress-prediction experiments. Code is only available on request, so it does not qualify as a public,作者Dataset · publicThe dataset used in this study was sourced from Kaggle and was publicly available.Open asset ↗Kagglepdf-page:16 lines:1-70Code / dataset availability confirmedOpenAlex · Europe PMC · checked 6 Sept 2026
Domain adaptation in agricultural settings has traditionally focused on 2D imagery, leaving a significant gap in the robust application of 3D sensing technologies for plant monitoring and classification. In this paper, we propose an adversarial unsupervised domain adaptation framework for 3D point cloud classification in agriculture, addressing the domain shift between controlled (Crops3D) and real-world (Pheno4D) datasets. Our approach leverages a PointNet-based feature extractor, a domain discriminator trained with a Gradient Reversal Layer (GRL), and an entropy minimization objective to ensure confident predictions on the unlabeled target domain. Extensive experiments demonstrate that our method achieves a classification accuracy of 97% on the target domain, with strong per-class F1 scores, despite significant sensor and environmental differences between datasets. We also evaluate model performance in real-time scenarios and discuss deployment feasibility on edge devices. This work highlights the potential of 3D domain adaptation in precision agriculture and paves the way for more generalizable plant phenotyping models.
Why it matches plant phenotyping methods3D点群分類のための敵対的ドメイン適応フレームワークを開発・評価しており、植物モニタリングおよび植物フェノタイピングへの適用が中心である。
abstractwe propose an adversarial unsupervised domain adaptation framework for 3D point cloud classification in agriculture
Reproduction assets foundThe paper uses two public 3D plant point cloud datasets as its source (Crops3D) and target (Pheno4D) domains; both are explicitly described as publicly available. No author code, trained models, or repository URLs are provided in the supplied blocks.Dataset · publicThe Crops3D dataset was collected by researchers at the Leibniz Institute of Agricultural Engineering and
Bioeconomy (ATB Potsdam) and made publicly available for academic research under an open data license.Open asset ↗pdf-page:5 lines:1-54Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Field / plotLeafClassificationStress / disease detectionDisease symptoms / severity
Existing disease discovery in papaya leaves is most significant in achieving yield and profitability stability in the tropics but has proven difficult in the presence of deficiencies in manual exploration and tailored crop models in crop-AI systems. Therefore, this study introduces PapayaNet, a lightweight attention-guided convolutional network specifically structured for the automated classification of six papaya leaf states, including major diseases and healthy leaves. For real-world deployment in scarce-resource farming contexts, PapayaNet adopts batch norm and hierarchical attention steps in five convolution stages and accelerates both computational celerity and discriminability. Trained on 6618 manually annotated orchard images sourced from orchards in Bangladesh at a very high resolution, it has a 98.79% classification accuracy, all of which was realized using 483,926 parameters and an average infer time of 0.01 s, which is significantly better when evaluated using EfficientNetB6, DenseNet121, and VGG16. XAI methods, including Grad-CAM and LIME, showed model decisions towards the biologically informative parts of the leaf, thus boosting interpretability and user confidence. Systematic ablation analysis also confirmed the importance of distributed attention in ensuring robust generalization towards visually similar disease classes. An in-browser diagnostic portal deployed using Gradio provides intra-browser predictive deployment and interpretability overlay in real time, thus inviting field practicability. Given its low-latency inference and minimal computational footprint, PapayaNet is well-suited for integration into edge devices and drone platforms, offering a scalable solution for real-time in-situ crop health monitoring. This study advances the field of precision agriculture by delivering a crop-specialized, explainable, and deployable AI system for sustainable management of papaya diseases.
Why it matches plant phenotyping methodsパパイヤ葉の病害・健全状態を画像から分類するCNN手法を開発し、データセット、比較評価、アブレーション、実運用ポータルまで中心的に扱っているため、植物病害フェノタイピング手法に該当する。
abstractthis study introduces PapayaNet, a lightweight attention-guided convolutional network specifically structured for the automated classification of six papaya leaf states, including major diseases and healthy leaves.
Reproduction assets foundThe paper's papaya leaf image dataset is publicly deposited on Mendeley Data with an explicit availability statement and authors' URL; no code or model checkpoint availability is stated.Dataset · publiccript.
Funding
This research did not receive any specific grant from funding agencies in the public, commercial, or not-for-profit sectors.
Data availability
The dataset analysed of this study, titled ”Healthy and Unhealthy Papaya Leaf Images from Bangladeshi Orchards,” is publicly available in the Mendeley Data repository at ( https://data.mendeley.com/datasets/44p8v6ywsm/1 ).
Competing interests
The authors declare no competing interests.
References
1. Sandhu, G. K. & Kaur, R. Plant disease detection techniques: A review. In 2019 International Conference on Automation, Computational and Technology Management, ICACTM 2019 34–38 (2019). 10.1109/ICACTM.2019.8776827
2.
Ngugi LC Abelwahab M AboOpen asset ↗Mendeley Datalines:622-669Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
In accordance with human life, agriculture has main role in it, and in addition to that most people are involved in some kind of agricultural activity either in a direct or indirect manner. Moreover, the agricultural sectors acquired a major role in supplying better quality food and thus made the greatest attribution to the growth of populations and economics. But, the disease over the crop has influenced the growth of the corresponding species and thus requires an earlier diagnosis of plant disease by utilizing the most adequate and automatic detection approach for improving the quality of the production of food as well as to reduce the loss in economic. But, there are no techniques in the conventional system for identifying the disease in diverse crops in the agricultural environment. In modern times, deep learning approaches have acquired tremendous enhancement in the identification of image categorization as well as the object detection system. For precise detection of plant disease, an improved classification model is developed. Initially, from the standard publicly available database, the images of the plants are aggregated. The gathered images are segmented using Dilated, Adaptive, and Attention-based Mask Recurrent Convolutional Neural Networks (DAA-MRCNN). Then, it is fed into a hybrid classification phase, where the new model namely Dilated, Adaptive, and Attention-based Multiscale DenseNet termed as (DAA-MDeNet) for classification. The classifier performance is improved by optimizing the parameter in Mask RCNN and Multiscale DenseNet using the hybrid optimization algorithm named African Vulture and Lemur Optimizer (AVLO). When compared with the other model, a superior performance is shown in the proposed model.
Why it matches plant phenotyping methods植物画像から病害をセグメンテーション・分類する深層学習手法を開発しており、罹病状態の推定が中心的な方法論的貢献である。
abstractFor precise detection of plant disease, an improved classification model is developed.
Reproduction assets foundThe paper uses the public PlantifyDr Kaggle dataset of plant disease images and provides the authors' implementation code on GitHub with explicit availability statements.Dataset · publica total of 12,500 images in it from 10 different plant types, where the 10 different types are considered as 10 individual datasets. (1) Apple, (2) Cherry, (3) Citrus, (4) Corn, (5) Grape, (6) Peach, (7) Pepper, (8) Potato, (9) Strawberry, and (10) Tomato. It contains a total of 37 as plant diseases. It was collected through “ https://www.kaggle.com/datasets/lavaman151/plantifydr-dataset ”: “Access Date: 2023-08-09”.
Thus, the images are significantly aggregated, and it has been termed as \documentclass[12pt]{minimal}
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\setlength{\oddsidemargin}{-Open asset ↗lines:103-129Code · publicThis research did not receive any specific funding.
Data availability
In case of benchmark data: The data underlying this article are available in the dataset link as: https://www.kaggle.com/datasets/lavaman151/plantifydr-dataset .
Code availability
The code for the implementation of the developed model is available at the link https://github.com/kalicharan8u/Plant-Disease-Detection-using-Mask-RCNN-with-Multiscale-DenseNet - and it has been given in Section " Simulation setup ".
Declarations
Competing interests
The authors declare no competing interests.
References
1.
Ashourloo D Matkan AA Huete A Aghighi H Mobasheri MR
Developing an index for detection and identification of disease stages
IOpen asset ↗lines:1529-1559Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Black gram, also known as urad bean, is an economically crucial crop widely cultivated in India, particularly in the central and southern regions. However, black gram is highly prone to multiple leaf diseases, resulting in considerable crop losses and economic challenges for farmers. Manual disease identification is slow and often unreliable, necessitating the development of automated disease detection methods. In this study, we propose ConViTSE, a lightweight hybrid deep learning architecture specifically designed for black gram leaf disease classification. ConViTSE integrates ConvMixer, Vision Transformer (ViT), and Squeeze and Excitation (SE) blocks to effectively extract and refine both local and global features. The model introduces Local Channel Attention Refinement (LCAR) and Global Channel Attention Refinement (GCAR) modules to enhance feature representation at different hierarchical levels. Extensive studies show that ConViTSE achieves a leading classification accuracy of 99.30% on the black gram dataset, outperforming traditional deep learning models. Furthermore, ConViTSE exhibits robust cross-domain generalization, achieving accuracies of 98.75% for rice, 98.20% for maize, and 95% for wheat, highlighting its potential for widespread adoption in precision agriculture. ConViTSE enhances disease detection accuracy while remaining computationally efficient, making it a practical tool for real-time disease management in diverse agricultural environments.
Why it matches plant phenotyping methods黒豆葉の病徴を画像から分類する深層学習手法の開発が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として適格です。
abstractwe propose ConViTSE, a lightweight hybrid deep learning architecture specifically designed for black gram leaf disease classification.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe Black Gram Plant Leaf Disease (BPLD) dataset from Mendeley Data49 is used in this work to classify leaf
diseases into five classes: anthracnose, healthy, leaf crinkle, powdery mildew, and yellow mosaic.Open asset ↗Mendeley Datapdf-page:14 lines:1-40Code / dataset availability confirmedCrossref · Europe PMC · checked 15 Sept 2026
Abstract The disease and pest recognition algorithms based on computer vision can automatically process and analyze a large amount of disease and pest images, thereby achieving rapid and accurate identification of disease and pest categories on crop leaves. Currently, most studies use deep learning models for feature extraction and identification of crop leaf disease and pest images. However, these methods are often seen as “black box” model, making it difficult to interpret the basis for their specific decisions. To address this issue, we propose an intrinsically interpretable crop leaf disease and pest identification model named C ontrastive P rototypical Part Net work (CPNet). The idea of CPNet is to find the key regions that influence the model’s decision by calculating the similarity values between the convolutional feature maps and the learnable latent prototype feature representations. Moreover, because the limited availability of data resources for crop leaf disease and pest images, we employ a supervised contrastive learning strategy to capture the similar information between examples in one class and contrast them with examples in other classes. Finally, we evaluate our approach on four publicly available datasets, and the experimental results demonstrate that our proposed CPNet not only achieves improvements in performance over baseline methods across multiple datasets, but also provides interpretable evidence for crop leaf disease and pest identification.
Why it matches plant phenotyping methods作物葉の病害状態を画像から推定する解釈可能なコンピュータビジョン手法を開発し、複数データセットで評価しており、病害表現型の取得・分類手法が中心です。
titleAn interpretable crop leaf disease and pest identification model based on prototypical part network and contrastive learning
Reproduction assets foundThe paper evaluates CPNet on four public leaf disease/pest image datasets. Two are directly linked in the Data availability statement: the Wheat Plant Diseases dataset (Kaggle) and the Dataset for Crop Pest and Disease Detection (Mendeley), both paper-specific image assets used for the phenotyping-style classification/Dataset · publicnstruction Project of Anhui Science and Technology University (Grant No. XK-XJGY002), the Anhui Provincial Department of Education Natural Science Major Project (Grant No. 2023AH040276).
Data availability
The data used in this study is publicly available at the following link: https://data.mendeley.com/datasets/bwh3zbpkpv/1 and https://www.kaggle.com/datasets/kushagra3204/wheat-plant-diseases .
Declarations
Competing interests
The authors declare no competing interests.
References
1.
Xing S Lee HJ
Crop pests and diseases recognition using DANet with TLDP
Comput. Electron. Agric. 2022 199 107144
10.1016/j.compag.2022.107144
Xing, S. & Lee, H. J. Crop pests and diseases recognition using DANetOpen asset ↗Kaggle · kushagra3204/wheat-plant-diseaseslines:1594-1660Dataset · publicversity (Grant No. FZ230122 ), the key Discipline Construction Project of Anhui Science and Technology University (Grant No. XK-XJGY002), the Anhui Provincial Department of Education Natural Science Major Project (Grant No. 2023AH040276).
Data availability
The data used in this study is publicly available at the following link: https://data.mendeley.com/datasets/bwh3zbpkpv/1 and https://www.kaggle.com/datasets/kushagra3204/wheat-plant-diseases .
Declarations
Competing interests
The authors declare no competing interests.
References
1.
Xing S Lee HJ
Crop pests and diseases recognition using DANet with TLDP
Comput. Electron. Agric. 2022 199 107144
10.1016/j.compag.2022.107144
Xing, S. & Lee, HOpen asset ↗Mendeley · bwh3zbpkpv/1lines:1594-1660Code / dataset availability confirmedCrossref · Europe PMC · checked 15 Sept 2026
Abstract The growth and productivity of banana crops are critically affected by micronutrient deficiencies, which are often difficult to detect at early stages. Lightweight deep learning models, optimized through neural architecture search (NAS) and attention mechanisms, are hypothesized to provide accurate and efficient classification of such deficiencies for real-time agricultural applications. In this study, multiple convolutional neural networks (CNNs) and mobile-friendly architectures, including ResNet50, VGG16, NASNetMobile, and MobileNet variants (V1, V2, V3), were evaluated using transfer learning on a curated banana leaf deficiency dataset. To improve robustness and prediction accuracy, modified classification layers and ensemble strategies–initially average ensembling and later a NAS-guided dynamic attention weighting mechanism were employed. This optimization resulted in a novel lightweight model, NASMobV2 (NASNetMobile + MobileNetV2), capable of both classifying nutrient deficiencies and assessing their severity levels. The proposed model achieved a validation accuracy of 98.57%, outperforming baseline and state-of-the-art counterparts in precision, recall, and F1 score. To improve generalization, banana crop diseases along with an additional Coffee crop dataset were included for evaluation. Finally, the practical utility of the model was demonstrated by deploying the trained system in both mobile and web applications, enabling farmers and agronomists to perform fast and accurate diagnostics directly in the field.
Why it matches plant phenotyping methodsバナナ葉画像から栄養欠乏と重症度を推定する軽量深層学習モデルを開発・評価し、実運用アプリにも展開しており、植物状態の取得・推定手法が中心である。
titleA neural architecture search optimized lightweight attention ensemble model for nutrient deficiency and severity assessment in diverse crop leaves.
Reproduction assets foundThe paper's banana leaf nutrient-deficiency image dataset is publicly available on Mendeley Data and was directly used for the phenotyping/classification measurements. No author analysis code or trained model checkpoints are explicitly deposited; the GitHub/Streamlit links are deployment apps rather than deposited codeDataset · publiclidation and editing in addition to overall supervision.
Funding
Open access funding provided by Vellore Institute of Technology. We thank our Management “Vellore Institute of Technology, Vellore” for open access funding support.
Data availability
An openly available repository (Mendeley dataset) was used to perform this study;(https://data.mendeley.com/datasets/7vpdrbdkd4/1), Request for any data or materials shall be addressed to the author(sudhakar.m2020@vitstudent.ac.in).
Declarations
Competing interests
The authors declare that they have no competing interests.
References
1.
Sherefu A Zewide I
Review paper on effect of micronutrients for crop production
J. Nutr. Food Process. 2021
10.31Open asset ↗Mendeley · 7vpdrbdkd4lines:1245-1307Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
The agriculture sector plays a pivotal role in the growth of the global economy, but remains highly susceptible to prediction errors, particularly in disease identification. To address the limitations of existing approaches, this study proposes a deep learning-based framework for the classification of medicinal plant leaf diseases. "Medicinal plant leaf images are collected from the standard data source. These images undergo a pre-processing phase that includes filtering and Contrast-Limited Adaptive Histogram Equalization (CLAHE) to enhance visual quality. Subsequently, an adaptive thresholding mechanism is employed for precise leaf segmentation. For effective disease recognition, deep feature extraction is carried out using a customized Multi-Scale VGG16 architecture," capturing diverse features such as color, shape, and texture. These heterogeneous features are then subjected to a weighted fused feature selection process, where feature weights are optimized using a novel Hybridized Zebra with Krill Herd Optimization (HZKHO) algorithm. The optimized feature set is input to the disease classification stage, which employs an Attention-based Dilated Adaptive DenseNet (A-DADensenet) model to produce accurate classification results. The proposed model achieves an impressive classification accuracy of 90.69%, thereby demonstrating its effectiveness in accurately identifying diseased medicinal plant leaves. "The integration of deep learning with a hybrid optimization technique" significantly enhances the model's classification performance, proving its potential for real-world agricultural applications.
Why it matches plant phenotyping methods薬用植物葉の画像から病害を分類する画像ベースの表現型推定手法を開発しており、葉の前処理・分割・特徴抽出・分類モデルが研究の中心である。
abstractthis study proposes a deep learning-based framework for the classification of medicinal plant leaf diseases.
Reproduction assets foundThe paper's medicinal plant leaf disease classification uses a public Mendeley Data image dataset (3838 soursop leaf images, six classes), explicitly cited with URL and access date in the Data availability statement. No author code or trained model is deposited.Dataset · publicThe medicinal plant leaf images are garnered from the dataset of Mendeley Data that is available in the data
source of https://data.mendeley.com/datasets/hjrhrt5hs8/2 with access date: 2025-07-21.Open asset ↗Mendeley Data · hjrhrt5hs8/2pdf-page:5 lines:1-34Code / dataset availability confirmedEurope PMC · Crossref · checked 6 Sept 2026
Accurate and early disease detection in paddy crops is essential for maximizing crop yield which ensures food security. Traditional methods are often labor-intensive, time-consuming, and domain-specific expertise. Feed-forward deep-learning models will perform accurate disease detection through the identification of spatial patterns. However, they cannot predict the diseases at the early stages due to the lack of temporal information. Temporal observations will help perform continuous monitoring and detect minute changes in the crops at the early times. To tackle this problem, we proposed Self-Supervised Deep Hierarchical Reconstruction (SSDHR), and Long Short-Term Memory (LSTM) which perform early disease detection based on the spatial and temporal data respectively. The SSDHR network uses multi-branch convolution kernels to extract distinct discriminative characteristics rather than conventional leaf-based indicators. It incorporates spatial, and temporal-based attention mechanism Symmetric Fusion Attention (SFA) to improve feature selection and XGBoost (XGB) classifier for better stability. According to experimental findings, the suggested framework achieves a 99.25% accuracy rate in identifying and classifying 13 paddy classes, including normal, blast, hispa, tungro, white stem borer, brown spot, leaf roller, downy mildew, yellow stem borer, bacterial leaf blight, bacterial leaf streak, black stem borer, and bacterial panicle blight.
Why it matches plant phenotyping methodsイネ病害の症状を空間・時間画像データから検出・分類する深層学習フレームワークを提案し、その性能を評価しているため、植物フェノタイピング手法が中心です。
abstractwe proposed Self-Supervised Deep Hierarchical Reconstruction (SSDHR), and Long Short-Term Memory (LSTM) which perform early disease detection based on the spatial and temporal data respectively.
Reproduction assets foundThe paper's phenotyping inputs are the publicly available Paddy Doctor image dataset (16,225 annotated paddy disease images) hosted on IEEE DataPort, with an explicit dataset link and data availability statement. No author analysis code or trained models are shared.Dataset · publicThe dataset used in our study was obtained from the publicly available repository titled “Paddy Disease and Pest Image Dataset” on IEEE Data Port. The dataset comprises 16,225 high-quality images across 13 classes, including 12 paddy disease and pest categories along with healthy samples.Open asset ↗IEEE Data Porthtml-lines:118-200Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Accurately detecting and counting potatoes during early harvest is essential for estimating yield, automating sorting, and supporting data-driven agricultural decisions. However, field environments often present practical challenges-such as soil occlusion, overlapping tubers, and inconsistent lighting-that hinder robust visual recognition. In response, we introduce SCG-YOLOv8n, a compact and field-adapted detection framework built upon the YOLOv8n architecture and specifically tailored for small-object detection in real-world farming conditions. The model incorporates three practical enhancements: a C-SPD module that preserves spatial detail to improve recognition of partially buried tubers; an S-CARAFE operator that reconstructs fine-scale features during upsampling; and GhostShuffleConv layers that reduce computational overhead without sacrificing accuracy. Through extensive field-based experiments, SCG-YOLOv8n consistently outperforms YOLOv5n and its base version across all key metrics. Float16 quantization compresses the model to 3.2 MB, enabling real-time inference on Android devices. We also developed PotatoDetector, a mobile application that demonstrates stable performance in field trials, achieving an RMSE of 1.38 and [Formula: see text] of 0.96 in counting tasks. These results suggest that SCG-YOLOv8n offers a practical and scalable tool for precision agriculture, with potential applicability to other root and tuber crop monitoring scenarios.
Why it matches plant phenotyping methodsジャガイモ塊茎の画像検出・計数を行うモデルとモバイル実装を開発し、圃場で性能検証している。塊茎数という植物器官形質の取得が中心であり、単なる収量測定ではない。
abstractwe introduce SCG-YOLOv8n, a compact and field-adapted detection framework built upon the YOLOv8n architecture and specifically tailored for small-object detection in real-world farming conditions.
Reproduction assets foundThe paper's custom potato image dataset is not publicly available (available only from the corresponding author on request), but the authors provide a public GitHub repository for the SCG-YOLOv8n analysis code with an explicit availability statement and URL.Code · publicCode availability
Code can be found at https://github.com/AiXia520/SCG-YOLOv8n.git.Open asset ↗https://github.com/AiXia520/SCG-YOLOv8n.githtml-lines:359-392Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Mango is a fruit of great economic importance in India. India is the top mango-producing nation in the world, accounting for over half of global mango output. In order to determine the production capability of the insured orchards, a complete inventory is carried out in situ every three years. The inventory includes counting number of trees, grouping them into yield categories, and assessing damaged ones. Satellite Remote Sensing proves to be a vital tool for estimating ecological parameters such as population density, tree health, volume, biomass, and carbon sequestration rates. The significance of tree counting extends beyond orchard evaluations, playing a vital role in environmental protection, agricultural planning, and crop yield forecast. unfortunately, conventional tree counting methods often require very expensive feature engineering, which leads to more errors as well as lower overall optimization. In order to overcome these obstacles, deep learning-based methods have been used to count trees, exhibiting cutting-edge results in this crucial activity. This paper introduces a novel approach employing deep learning for Image-Based Mango Tree counting in high-resolution satellite imagery data. The proposed model, named Bi-directional Feature Pyramid Network (BiFPN)-YOLOv8m an improved version of YOLOv8, employs object detection to effectively separate, locate, and count mango trees with in orchards. A dataset of 1700 training and 300 testing images of mango orchards with trees of various ages is used to evaluate the various YOLOv8 variants, YOLOv8n, YOLOv8s, YOLOv8m, YOLOv8l, YOLOv8x, including YOLOv9, YOLOv10, and BiFPN-YOLOv8m, with a focus on computational efficiency, accuracy, and speed. Experimental findings show that, even under difficult circumstances, the proposed method continuously outperforms state-of-the-art techniques.
Why it matches plant phenotyping methods衛星画像からマンゴー樹木を分離・位置推定・計数する深層学習手法を開発・評価しており、植物個体数という観測可能な形態・構造形質の抽出が中心である。
abstractThis paper introduces a novel approach employing deep learning for Image-Based Mango Tree counting in high-resolution satellite imagery data.
Reproduction assets foundThe paper's satellite remote sensing image dataset used for mango tree counting is publicly deposited on GitHub per the Data Availability Statement. No separate analysis code or trained model checkpoint is explicitly deposited.Dataset · publicRemote Sensing Image Data that support the findings of this study have been deposited in the GitHub. The url to the data uploaded is https://github.com/lbirla/Mango_tree_satellite_data.Open asset ↗https://github.com/lbirla/Mango_tree_satellite_datahtml-lines:497-525Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Citrus fruits, especially lemons, play a vital economic and nutritional role worldwide but are increasingly threatened by a wide range of diseases that diminish yield quality and quantity. Traditional manual and automated methods for disease detection requires domain expert, ample observation time, and is often ineffective during early infection stages. This paper presents a novel automated approach for the symptom based detection and classification of citrus leaf diseases using a nonlinear Fuzzy Rank-Based Ensemble (NL-FuRBE) methodology, enhanced by image quality improvement techniques. The study emphasizes the significance of timely disease diagnosis in citrus crops, which are vital for global food security and economic stability. The methodology begins with image quality enhancement through Vector-Valued Anisotropic Diffusion (VAD) and morphological filtering, evaluated using PSNR, SSIM, and NIQE metrics to ensure optimal visual clarity for classifier input. The core ensemble integrates three deep learning (DL) architectures-VGG19, AlexNet, and Xception-using a fuzzy rank-based scoring mechanism built on nonlinear transformations (exponential, tanh, and sigmoid functions) to address prediction uncertainty and model bias. A comprehensive dataset of lemon leaf diseases, consisting of 1354 images across nine classes, was utilized for training and evaluation. Experimental results using five-fold cross-validation demonstrate that the proposed model achieves superior performance with an average accuracy of 96.51%, outperforming conventional ensemble and state-of-the-art approaches. The results validate the proposed NL-FuRBE as an effective, automated, and cost-efficient tool for precision agriculture and early disease diagnosis in citrus farming.
Why it matches plant phenotyping methods柑橘葉の病徴を画像から検出・分類する手法を新規開発し、画像処理、アンサンブル分類、交差検証で技術性能を評価しているため、植物病害状態の表現型取得が中心である。
abstractThis paper presents a novel automated approach for the symptom based detection and classification of citrus leaf diseases using a nonlinear Fuzzy Rank-Based Ensemble (NL-FuRBE) methodology, enhanced by image quality improvement techniques.
Reproduction assets foundThe paper's lemon leaf disease image dataset (1354 images, nine classes) is publicly deposited on Mendeley Data with explicit availability statement and DOI; no author code or models are shared.Dataset · publicThe dataset used during the current study are available in the Mendeley Data repository under the title “Comprehensive Lemon Leaf Disease Dataset for Advanced Detection and Sustainable Agriculture” (DOI: 10.17632/44nrn4593f.1), https://data.mendeley.com/datasets/44nrn4593f/1.Open asset ↗Mendeley Data · 10.17632/44nrn4593f.1html-lines:805-842Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Deep learning models have shown remarkable success in disease detection and classification tasks, but lack transparency in their decision-making process, creating reliability and trust issues. Although traditional evaluation methods focus entirely on performance metrics such as classification accuracy, precision and recall, they fail to assess whether the models are considering relevant features for decision-making. The main objective of this work is to develop and validate a comprehensive three-stage methodology that combines conventional performance evaluation with qualitative and quantitative evaluation of explainable artificial intelligence (XAI) visualizations to assess both the accuracy and reliability of deep learning models. Eight pre-trained deep learning models - ResNet50, InceptionResNetV2, DenseNet 201, InceptionV3, EfficientNetB0, Xception, VGG16 and AlexNet,were evaluated using a three-stage methodology. First, the models are assessed using traditional classification metrics. Second, Local Interpretable Model-agnostic Explanations (LIME) is employed to visualize and quantitatively evaluate feature selection using metrics such as Intersection over Union (IoU) and the Dice Similarity Coefficient (DSC). Third, a novel overfitting ratio metric is introduced to quantify the reliance of the models on insignificant features. In the experimental analysis, ResNet50 emerged as the most accurate model, achieving 99.13% classification accuracy as well as the most reliable model demonstrating superior feature selection capabilities (IoU: 0.432, overfitting ratio: 0.284). Despite the high classification accuracies, models such as InceptionV3 and EfficientNetB0 showed poor feature selection capabilities with low IoU scores (0.295 and 0.326) and high overfitting ratios (0.544 and 0.458), indicating potential reliability issues in real-world applications. This study introduces a novel quantitative methodology for evaluating deep learning models that goes beyond traditional accuracy metrics, enabling more reliable and trustworthy AI systems for agricultural applications. This methodology is generic and researchers can explore the possibilities of extending it to other domains that require transparent and interpretable AI systems.
Why it matches plant phenotyping methodsイネ葉の病徴を画像から検出・分類する深層学習モデルについて、性能と説明可能性を評価する三段階の方法論を開発・検証しており、植物病害状態のフェノタイピング手法が中心である。
abstractThe main objective of this work is to develop and validate a comprehensive three-stage methodology that combines conventional performance evaluation with qualitative and quantitative evaluation of explainable artificial intelligence (XAI) visualizations to assess both the accuracy and reliability of deep learning models.
Reproduction assets foundThe paper uses the public Kaggle rice leaf disease dataset and explicitly states that the authors' analysis code is publicly available on GitHub and MATLAB File Exchange via short URLs, both of which are in the allowed URL list.Dataset · publicChinna Gopi Simhadri: Formal analysis, investigation, resources, data collection, writing an original draft, Review & editing. All authors have read and agreed to the published version of the manuscript.
Data availability
In this work, the publicly available dataset was used. The data set is available through the link to Kaggle https://www.kaggle.com/datasets/dedeikhsandwisaputra/rice-leafs-disease-dataset.
Code availability
The code developed and used in this study has been uploaded and is available on GitHub and MATLAB File Exchange. These repositories include all the necessary scripts, tools, and instructions required to replicate the results presented in this work. The repository can be Open asset ↗Kaggle · dedeikhsandwisaputra/rice-leafs-disease-datasetlines:1332-1347Code · publicode availability
The code developed and used in this study has been uploaded and is available on GitHub and MATLAB File Exchange. These repositories include all the necessary scripts, tools, and instructions required to replicate the results presented in this work. The repository can be accessed via the following links: Github: https://shorturl.at/Github_Quant_XAI MATLAB File Exchange link: https://shorturl.at/MATLAB_Quant_XAI
Declarations
Competing interestsOpen asset ↗GitHublines:1332-1347Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Potato blight is a serious disease that affects potato crops and leads to substantial agricultural and economic losses. To enhance detection accuracy, we propose Bayesian Optimized CNN Weighted Ensemble Potato Blight Detection, a deep learning-based approach that optimizes CNN models through Bayesian optimization and ensemble learning. In the proposed study, extensive experiments were conducted to develop an optimized Bayesian Weighted Ensemble CNN model for the detection of potato leaf blight. First, multiple CNN architectures were trained using different optimizers: ADAM (DL1), SGD (DL2), RMSProp (DL3), and ADAMAX (DL4), evaluating their individual performance. To mitigate class imbalance, data augmentation techniques were applied, increasing the number of healthy leaves by 6 times. In addition, fuzzy image enhancement was implemented to improve feature extraction and classification accuracy. Bayesian optimization was then used to determine the optimal weights for a deep ensemble model, exploring 11 possible model combinations. The final EDL7 ensemble model (DL1 + DL2 + DL3), optimized through Bayesian optimization, achieved the highest accuracy of 97.94%, outperforming individual models. Furthermore, the ensemble model achieved a precision of 0.981, recall of 0.983, and an F1 score of 0.982, ensuring a well-balanced trade-off between precision and recall. These results highlight the effectiveness of Bayesian-optimized ensemble learning in improving potato blight detection, making it a robust and reliable solution for agricultural disease classification.
Why it matches plant phenotyping methodsジャガイモ葉の病徴を画像から分類するCNN・画像強調・ベイズ最適化アンサンブルを中心に開発・評価しており、植物病害状態の取得手法が主題である。
abstractwe propose Bayesian Optimized CNN Weighted Ensemble Potato Blight Detection, a deep learning-based approach that optimizes CNN models through Bayesian optimization and ensemble learning.
Reproduction assets foundThe paper's potato leaf blight detection models were trained on a public Kaggle dataset, explicitly declared in the Data availability statement. No author analysis code or trained model checkpoints are reported as publicly available.Dataset · publicThe datasets generated and analyzed during the current study are available in the Kaggle repository https://www.kaggle.com/datasets/muhammadardiputra/potato-leaf-disease-datasetOpen asset ↗Kaggle · muhammadardiputra/potato-leaf-disease-datasetlines:308-345Code / dataset availability confirmedCrossref · Europe PMC · checked 6 Sept 2026
Abstract Crop disease is a significant challenge in agriculture, requiring quick and precise detection to safeguard yields and reduce economic losses. Traditional diagnostic methods are slow, labor-intensive, and rely on expert knowledge, limiting scalability for large-scale operations. To overcome these challenges, a novel architecture called Mob-Res , combining residual learning with the MobileNetV2 feature extractor, is introduced in this work. Despite having only 3.51 million parameters, Mob-Res is lightweight and well-suited for mobile applications while delivering exceptional performance. The proposed model is assessed using two benchmark datasets: Plant Disease Expert , consisting of 199,644 images across 58 classes, and PlantVillage , with 54,305 images across 38 classes. Through a rigorous training strategy, Mob-Res demonstrates robust performance, achieving 97.73% average accuracy on the Plant Disease Expert dataset and 99.47% on the PlantVillage dataset. The cross-domain validation rate ( CDVR ) is computed to assess its cross-domain adaptability, with the model showing competitive results compared to other pre-trained models. Additionally, Mob-Res outperforms prominent pre-trained CNN architectures, surpassing ViT-L32 while maintaining a significantly lower parameter count and achieving faster inference times. The proposed model enhances interpretability by utilizing Gradient-weighted Class Activation Mapping ( Grad-CAM ), Grad-CAM++ , and Local Interpretable Model-agnostic Explanations ( LIME ). These techniques provide visual insights into the neural regions influencing the predictions. The experimental results conducted in the current work highlight Mob-Res as a promising solution for automated plant disease detection, supporting large-scale agricultural operations and advancing global food security.
Why it matches plant phenotyping methods植物画像から病害状態を推定するCNNを開発し、複数データセットで性能比較・検証しているため、画像ベースの植物フェノタイピング手法が中心である。
titleA lightweight and explainable CNN model for empowering plant disease diagnosis
Reproduction assets foundThe paper's authors publicly host the Mob-Res architecture code on GitHub, and the study directly uses two public plant-disease image datasets (PlantVillage and the Mendeley Sugarcane Leaf Disease Dataset) for its phenotyping/classification experiments. The Plant Disease Expert dataset (Kaggle) is also used but its URLCode · publicThe code of the architecture
is available at https://github.com/Chiranjit369/Mob-Res.Open asset ↗Chiranjit369/Mob-Respdf-page:6 lines:1-71Dataset · publicwe have used a benchmark Sugarcane Leaf Disease Dataset as mentioned in Section Experiments on field
dataset which can be accessed at https://data.mendeley.com/datasets/9424skmnrk/1.Open asset ↗pdf-page:13 lines:1-60Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
The biodiversity function of the desert steppe ecosystem faces many challenges under the pressure of climate change and human activities. Accurate and efficient assessment of plant diversity is critical for guiding desert steppe restoration efforts. However, desert steppe vegetation has sparse leaves and sparse distribution. It is difficult to accurately distinguish micro-vegetation types based on a single spectrum, vegetation index or texture feature, and the resolution of satellite remote sensing cannot meet the needs of high-precision diversity assessment. To this end, this study proposed a novel method for assessing plant diversity index in degraded desert grassland based on multimodal UAV hyperspectral data and Encoder-CNN. Through experiments on different modal feature combinations, spatial spectra, vegetation indices and texture features were targeted and fused. Channel Attention Fusion (CAF) was introduced into Encoder to achieve cross-layer "soft" residual fusion, the Encoder and CNN models were fused to construct a global-local co-expression structure, and finally the quantitative calculation of the plant diversity index at the pixel level was realized. The results show that the vegetation types determined by the fusion of multimodal data and deep learning are consistent with the existing species, dominant species and sub-dominant species of the actual community, and the calculated diversity index results are also consistent with the actual situation. The use of multimodal data combining spatial spectral features with index features, combined with the Encode-CNN model, can provide the most accurate information on community composition. The overall accuracy of sparse vegetation classification can reach 90.01%, and the average accuracy can reach 85.23%, which is better than single mode or traditional 3DCNN, VIT models. This study demonstrates the application potential of UAV hyperspectral multimodal technology and deep learning in the assessment of desert steppe plant diversity, providing important technical support for ecological protection and conservation.
Why it matches plant phenotyping methodsUAVハイパースペクトルとEncoder-CNNを用いて、植物多様性指数を画素レベルで定量推定する手法を開発・評価しており、植物状態の取得・抽出が研究の中心である。
abstractthis study proposed a novel method for assessing plant diversity index in degraded desert grassland based on multimodal UAV hyperspectral data and Encoder-CNN.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publicThe codes used in this study are available at https://github.com/15204718180/encoder-cnn.Open asset ↗15204718180/encoder-cnnpdf-page:17 lines:56-74Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
The vigor of potato plants is of crucial importance for potato seed producers, who are interested in predicting it at scale by exploiting the dependence of plant growth and development on the origin and physiological state of the seed tuber. In this article we present the results of a three-year long experiment in which we studied six potato varieties in three test fields. We identify a 73-[Formula: see text] overall correlation in the vigor of plants from the same seedlot grown in different test fields. Similarly, the biochemical tuber data produce plant vigor predictions that correlate up to 70-[Formula: see text] with the measurements. However, these relatively large data and prediction correlations are mostly due to the strong dependence of the seedlot vigor on the tuber genotype. For five out of six studied varieties, variety-specific cross-field and cross-year vigor predictions produce negligible or even negative correlations when the seed tubers and young plants experience environmental stress. At the same time, for the variety that appeared to be less sensitive to environmental stresses, we obtained cross-field and cross-year vigor predictions correlating up to [Formula: see text] with the measurements. Analysis of individual predictor variables, such as the abundance of a particular metabolite, indicates that the vigor-enhancing properties of the seed tubers are also variety-specific and that the FTIR spectroscopy data is the most reliable predictor.
Why it matches plant phenotyping methodsFTIR・生化学データからジャガイモ植物の vigor を予測し、圃場間・年次間で予測性能を検証しており、植物形質の取得・推定法が中心です。
abstractinterested in predicting it at scale
Reproduction assets foundThe paper's Data Availability statement points to a public 4TU.ResearchData deposit containing the seed tuber and plant canopy (drone-derived vigor) datasets plus the Python code needed to reproduce the regression results — a paper-specific, publicly actionable asset.Dataset · publicBoth the seed tuber and plant canopy datasets are available at http://doi.org/10.4121/3a97fa0c-8c7d-451a-b8fe-d521f1cec55e . The data also includes the Python code necessary to reproduce the results of regression.Open asset ↗10.4121/3a97fa0c-8c7d-451a-b8fe-d521f1cec55elines:267-336Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Precision and timeliness in the detection of plant diseases are important to limit crop losses and maintain global food security. Much work has been performed to detect plant diseases using deep learning methods. However, deep learning techniques demand a large quantity of data to train the models for diagnosis and further classification. Few-shot learning has surfaced to remove the drawbacks of deep learning methods. Therefore, the proposed work presents a novel GRCornShot model for corn disease diagnosis using few-shot learning with Prototypical Networks based on metric learning. Metric Learning calculates the distance to measure the similarity between the data points. Hence, addressing the challenge of limited labeled data, GRCornShot effectively classifies healthy and corn diseases. Furthermore, the Gabor filter is incorporated into the backbone network ResNet-50 to extract the texture features and to enhance the classification performance. The experiments show the promising application of few-shot learning in agronomic applications, providing a robust solution for detecting corn diseases precisely with minimal data requirements. Using a 4-way 2-shot, 3-shot, 4-shot, and 5-shot learning strategy, GRCornShot achieves impressive accuracy of 96.19%, 96.54%, 96.90%, and 97.89%, respectively.
Why it matches plant phenotyping methodsトウモロコシ葉の病害状態を画像から分類する少数ショット深層学習手法を開発し、精度を評価しており、植物フェノタイピング手法が中心である。
abstractthe proposed work presents a novel GRCornShot model for corn disease diagnosis using few-shot learning with Prototypical Networks based on metric learning.
Reproduction assets foundThe paper's corn disease detection experiments use two publicly available image datasets (Roboflow corn-disease and the Kaggle corn/maize leaf disease dataset), explicitly linked in the Data Availability statement. No author analysis code or trained model checkpoint is deposited.Dataset · publiction, Writing—Original draft preparation. J.S. Conceptualization of this study, Editing, Supervision. S.B. Conceptualization of this study, Editing, Supervision. S.K. Funding, Editing, Supervision. All authors reviewed the manuscript.
Data availability
Publicly available datasets were used in this study which can be found here: https://universe.roboflow.com/final-enlye/corn-disease and https://www.kaggle.com/datasets/smaranjitghose/corn-or-maize-leaf-disease-dataset.
Declarations
Competing interests
The authors declare no competing interests.
Footnotes
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
JayakOpen asset ↗Roboflow · final-enlye/corn-diseaselines:355-376Dataset · publication of this study, Editing, Supervision. S.B. Conceptualization of this study, Editing, Supervision. S.K. Funding, Editing, Supervision. All authors reviewed the manuscript.
Data availability
Publicly available datasets were used in this study which can be found here: https://universe.roboflow.com/final-enlye/corn-disease and https://www.kaggle.com/datasets/smaranjitghose/corn-or-maize-leaf-disease-dataset.
Declarations
Competing interests
The authors declare no competing interests.
Footnotes
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Jayakrushna Sahoo, Sivaiah Bellamkonda and Sumit Kumar contributOpen asset ↗Kaggle · smaranjitghose/corn-or-maize-leaf-disease-datasetlines:355-376Code / dataset availability confirmedEurope PMC · Crossref · checked 14 Sept 2026
Precise classification and detection of apple diseases are essential for efficient crop management and maximizing yield. This paper presents a fine-tuned EfficientNet-B0 convolutional neural network (CNN) for the automated classification of apple leaf diseases. The model builds upon a pre-trained EfficientNet-B0 base, enhanced through architectural modifications such as the integration of a global max pooling (GMP) layer, dropout, regularization, and full-model fine-tuning. To address class imbalance and improve generalization, the study adopts a holistic training strategy that integrates data augmentation, stratified data splitting, and class weighting, alongside transfer learning. The model is evaluated on the PlantVillage (PV) dataset and a curated Apple PV (APV) dataset and compared against EfficientNet-B0, EfficientNet-B3, Inception-v3, ResNet50, and VGG16 models. The fine-tuned model demonstrates outstanding test accuracies of 99.69% and 99.78% for classifying plant diseases using the APV and PV datasets, respectively. The fine-tuned model outperforms EfficientNet-B0, EfficientNet-B3, and VGG16 on both datasets and shows superior performance compared to Inception-v3 and ResNet-50 on the PV dataset. Both EfficientNet-B0 and the fine-tuned model demonstrate the lowest memory consumption and floating-point operations per second (FLOPs). Also, as compared to the EfficientNet-B0 model, the fine-tuned model achieves an 11% increase in accuracy on the APV dataset and a 49.5% accuracy improvement on the PV dataset, with approximately a 7-8% increase in both memory usage and FLOPs. The fine-tuned model thus emerges as an effective solution for plant leaf disease classification, delivering outstanding accuracy with optimized memory consumption and FLOPs, making it suitable for resource-constrained environments. This study demonstrates that fine-tuned CNN approaches, when combined with transfer learning, advanced data pre-processing, and architectural optimizations, can significantly enhance the accuracy of diseased leaf classification in crops with efficient implementation in limited-resource settings.
Why it matches plant phenotyping methodsリンゴ葉の病害状態を画像から分類するCNN手法の開発・比較評価が研究の中心であり、植物病害表現型の取得・推定に該当する。
abstractThis paper presents a fine-tuned EfficientNet-B0 convolutional neural network (CNN) for the automated classification of apple leaf diseases.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicThe PV dataset used for this research work is taken from: https://data.mendeley.com/datasets/tywbtsjrjv/1Open asset ↗tywbtsjrjv/1lines:334-374Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
With the increasing demand for precision agriculture, automatic detection of tomato leaf diseases has become a critical technological challenge in smart agriculture. Among various diseases, Tomato Yellow Virus Leaf, due to its unique pathological characteristics, presents a particularly challenging identification target. Traditional image recognition methods often fail to meet the high-precision detection requirements for this disease, leading to delayed responses in disease control by farmers, which severely impacts tomato yield and quality. To address this issue, this paper proposes an optimized YOLOv8n algorithm, incorporating a C2f-DynamicConv optimization module. By dynamically adjusting the weights of convolutional kernels, the model can adapt to the characteristics of different input data, thereby enhancing its ability to represent diverse features. Additionally, we introduce the SimAM attention mechanism, which enhances the model's focus on key areas by weighting the feature map, significantly improving the accuracy of disease detection while filtering out irrelevant features and enhancing sensitivity. During the upsampling process, we adopt the Dysample upsampling operator, optimizing the quality of feature map reconstruction and improving detection resolution through a refined upsampling strategy. To better address the bounding box regression problem in object detection, we incorporate the GIoU loss function. Compared to traditional loss functions, GIoU performs excellently in handling bounding box overlap and positional accuracy, further improving the model's detection performance. Experimental results show that the improved model achieves an average precision of 81.8%, precision of 77.1%, and recall of 77.4%. Compared to existing methods, our approach shows significant advantages in detection accuracy, localization precision, and model computational efficiency, achieving improved detection performance on the tomato leaf disease dataset.
Why it matches plant phenotyping methodsトマト葉の病徴を画像から検出する改良YOLOv8n手法を開発し、精度・再現率・位置特定性能を評価しており、植物の病害状態の取得が研究の中心である。
abstractExperimental results show that the improved model achieves an average precision of 81.8%, precision of 77.1%, and recall of 77.4%.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicData availability
The datasets can be downloaded in https://github.com/weilaibot/sanyau_02.git.Open asset ↗weilaibot/sanyau_02pdf-page:20 lines:1-65Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Plant diseases pose a significant threat to global food security, with severe implications for agricultural productivity. Early and accurate detection of these diseases is crucial, yet it remains a challenging task, significantly impacting crop yields and food supply chains. Despite the progress in artificial intelligence, particularly deep learning, challenges persist in real-world applications due to environmental noise, varying light conditions, and other complicating factors that hinder detection accuracy. This study introduces the AttCM-Alex model, a novel deep-learning framework designed to boost the detection and classification of plant diseases under challenging environmental conditions. By integrating convolutional operations with self-attention mechanisms, AttCM-Alex effectively addresses the variability in light intensity and image noise, ensuring robust performance. To simulate practical agricultural scenarios, the study employs bilinear interpolation for image dimension adjustment and introduces Salt-and-Pepper noise. Additionally, the model's robustness was evaluated by varying image brightness levels by ±10%, ±20%, and ±30%. Experimental results demonstrate that AttCM-Alex significantly outperforms traditional models, particularly in scenarios involving fluctuating light conditions and noise interference. The model achieved a peak detection accuracy of 0.97 with a 30% increase in image brightness and maintained an accuracy of 0.93 even with a 30% decrease in brightness, highlighting its robustness and reliability. The findings affirm the AttCM-Alex model as a powerful tool for real-world agricultural applications, capable of enhancing disease detection systems' accuracy and efficiency. This advancement not only supports better crop management practices but also contributes to sustainable agriculture and global food security.
Why it matches plant phenotyping methods植物画像から病害を検出・分類する深層学習手法を開発し、照明変動やノイズ条件で性能評価しており、病害状態の取得・推定が研究の中心である。
abstractThis study introduces the AttCM-Alex model, a novel deep-learning framework designed to boost the detection and classification of plant diseases under challenging environmental conditions.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publicThe proposed model codes are publicly available at: https://github.cOpen asset ↗pdf-page:5 lines:1-71Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
The increasing global population, coupled with the diminishing availability of arable land, has rendered the challenge of ensuring food security more pronounced. The prompt and precise identification of plant diseases is essential for reducing crop losses and improving agricultural yield. This paper introduces the Swin Transformer with Convolutional Feature Interactions (ST-CFI), a state-of-the-art deep learning framework designed for detecting plant diseases through the analysis of leaf images. The ST-CFI model effectively integrates the strengths of the Convolutional Neural Networks (CNNs) and Swin Transformers, enabling the extraction of both local and global features from plant images. This is achieved through the implementation of an inception architecture and cross-channel feature learning, which collectively enhance the information necessary for detailed feature extraction. Comprehensive experiments were conducted using five distinct datasets: PlantVillage, Plant Pathology 2021 competition dataset, PlantDoc, AI2018, and iBean. The ST-CFI model exhibited exceptional performance, achieving an accuracy of 99.96% on the PlantVillage dataset, 99.22% on iBean, 86.89% on AI2018, and 77.54% on PlantDoc. These results underscore the model's robustness and its capacity to generalize across various datasets and real-world conditions. The high accuracy and F1 scores, in conjunction with low loss values, further validate the model's efficacy in learning discriminative features. The ST-CFI model signifies a substantial advancement in the early and accurate detection of plant diseases, serving as a valuable instrument for precision agriculture. Its capacity to integrate CNNs and Transformers within a unified framework enhances the model's feature extraction capabilities, resulting in improved accuracy in the identification of plant diseases. This study concludes that the ST-CFI model effectively addresses plant disease detection challenges, with significant implications for agricultural sustainability and productivity.
Why it matches plant phenotyping methods葉画像から植物病害を識別する深層学習手法を提案し、複数データセットで性能評価しているため、植物状態の画像ベース表現型推定が中心である。
abstractThis paper introduces the Swin Transformer with Convolutional Feature Interactions (ST-CFI), a state-of-the-art deep learning framework designed for detecting plant diseases through the analysis of leaf images.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicAll data sets in the paper are public data sets, and the corresponding data sets can be downloaded according to
the references provided.Open asset ↗pdf-page:18 lines:1-26Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Cotton, the backbone of global textile production, demands sustainable agricultural practices to ensure fiber, food, and environmental security. Cotton crop play an essential role in farming economies; however, production is sometimes affected by various diseases that harm production. We proposed a methodology that uses formal modeling and verification for requirements confirmation to improve the monitoring and detection of cotton crop diseases. The correct information and requirements about disease symptoms can improve disease monitoring and prediction. The Temporal Logic of Action (TLA+) is used to construct a mathematical model to verify requirements by providing disease symptoms and then model checking to ensure correctness properties. Using model checking in TLA + ensures the reliability and correctness of disease symptom detection. We consequently used deep learning models to predict cotton diseases, i.e., Aphids, Armyworms, Bacterial Blight, Powdery Mildew, Target Spot, and Healthy leaf. Our results show that the Convolutional Neural Network (CNN) model achieved an overall accuracy of 98.7% with class-specific accuracy ranging from with F1-scores across all classes (e.g., 0.90 for Powdery Mildew and 0.87 for Army Worm).
Why it matches plant phenotyping methods綿花葉の病徴を画像から深層学習で分類・検出する手法が研究の中心であり、植物の病害状態を推定するため、植物フェノタイピング手法として収録対象。
abstractWe proposed a methodology that uses formal modeling and verification for requirements confirmation to improve the monitoring and detection of cotton crop diseases.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publictoolbox modules, are publicly available on GitHub at https://github.com/abdulpk/MuhammadMusa for furtherOpen asset ↗https://github.com/abdulpk/MuhammadMusapdf-page:18 lines:1-64Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Plant diseases pose significant challenges to farmers and the agricultural sector at large. However, early detection of plant diseases is crucial to mitigating their effects and preventing widespread damage, as outbreaks can severely impact the productivity and quality of crops. With advancements in technology, there are increasing opportunities for automating the monitoring and detection of disease outbreaks in plants. This study proposed a system designed to identify and monitor plant diseases using a transfer learning approach. Specifically, the study utilizes YOLOv7 and YOLOv8, two state-of-the-art models in the field of object detection. By fine-tuning these models on a dataset of plant leaf images, the system is able to accurately detect the presence of Bacteria, Fungi and Viral diseases such as Powdery Mildew, Angular Leaf Spot, Early blight and Tomato mosaic virus. The model's performance was evaluated using several metrics, including mean Average Precision (mAP), F1-score, Precision, and Recall, yielding values of 91.05, 89.40, 91.22, and 87.66, respectively. The result demonstrates the superior effectiveness and efficiency of YOLOv8 compared to other object detection methods, highlighting its potential for use in modern agricultural practices. The approach provides a scalable, automated solution for early any plant disease detection, contributing to enhanced crop yield, reduced reliance on manual monitoring, and supporting sustainable agricultural practices.
Why it matches plant phenotyping methods植物葉画像から病害の有無・種類を推定する画像ベースの病害表現型計測法をYOLOモデルで開発・評価しており、手法が研究の中心です。
abstractThis study proposed a system designed to identify and monitor plant diseases using a transfer learning approach.
Reproduction assets foundThe paper's Data Availability statement provides a public Google Drive link to the study data and a public GitHub repository, and the study's input images come from the public Roboflow 'Detecting Diseases' dataset. These are paper-specific, publicly accessible assets supporting the plant disease detection experiments.Dataset · publicone who inspired our work.
Author contributions
BSM: conceptualization and drafting, HSN: data collection and analysis. MNW: Methodology and review. NOF: Drafting and interpretation. CCZ: Writing and method. HDA: writing and Data Collection; EMO: Drafting, Supervision and Editing.
Data availability
The data can be accessed via: https://drive.google.com/file/d/1kA_JWhHQhyzzuzlpzppK2nNTtbiR2N77/view . https://github.com/Sachinthana-Lokuyaddage/Plant_Disease_Detection_Using_Transfer_Learning_with_ResNet50 .
Declarations
Competing interests
The authors declare no competing interests.
Consent to participate
All authors consent to participate.
Footnotes
Publisher’s note
Springer Nature remains neuOpen asset ↗lines:209-247Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Agricultural diseases pose significant challenges to plant production. With the rapid advancement of deep learning, the accuracy and efficiency of plant disease identification have substantially improved. However, conventional convolutional neural networks that rely on multi-layer small-kernel structures are limited in capturing long-range dependencies and global contextual information due to their constrained receptive fields. To overcome these limitations, this study proposes a plant disease recognition method based on RepLKNet, a convolutional architecture with large kernel designs that significantly expand the receptive field and enhance feature representation. Transfer learning is incorporated to further improve training efficiency and model performance. Experiments conducted on the Plant Diseases Training Dataset, comprising 95,865 images across 61 disease categories, demonstrate the effectiveness of the proposed method. Under five-fold cross-validation, the model achieved an overall accuracy (OA) of 96.03%, an average accuracy (AA) of 94.78%, and a Kappa coefficient of 95.86%. Compared with ResNet50 (OA: 95.62%) and GoogleNet (OA: 94.98%), the proposed model demonstrates competitive or superior performance. Ablation experiments reveal that replacing large kernels with 3×3 or 5×5 convolutions results in accuracy reductions of up to 1.1% in OA and 1.3% in AA, confirming the effectiveness of the large kernel design. These results demonstrate the robustness and superior capability of RepLKNet in plant disease recognition tasks.
Why it matches plant phenotyping methods植物病害状態を画像から認識する深層学習手法の開発と比較検証が研究の中心であり、植物フェノタイプ（病害状態）の抽出に該当する。
abstractthis study proposes a plant disease recognition method based on RepLKNet
Reproduction assets foundThe paper's plant disease image dataset (Plant Diseases Training Dataset, 95,865+ images across 61 disease categories) is explicitly stated to be publicly available on Kaggle, with the exact URL matching an allowed URL. No author analysis code or trained model checkpoints are reported.Dataset · publicThe dataset used for this model is called the Plant Diseases Training Dataset, available on Kaggle at https://www.kaggle.com/code/gpiosenka/efficentnet-mobilenet-f1-s-93-93/input .Open asset ↗Kagglelines:83-103Code / dataset availability confirmedCrossref · Europe PMC · checked 6 Sept 2026
Abstract There are some key problems faced in modern agriculture that IoT-based smart farming. These problems such shortage of water, plant diseases, and pest attacks. Thus, artificial intelligence (AI) technology cooperates with the Internet of Things (IoT) toward developing the agriculture use cases and transforming the agriculture industry into robustness and ecologically conscious. Various IoT smart agriculture techniques are escalated in this field to solve these challenges such as drop irrigation, plant diseases detection, and pest detection. Several agriculture devices were installed to perform these techniques on the agriculture field such as drones and robotics but in expense of their limitations. This paper proposes an AI-IoT smart agriculture pivot as a good candidate for the plant diseases detection and treatment without the limitations of both drones and robotics. Thus, it presents a new IoT system architecture and a hardware pilot based on the existing central pivot to develop deep learning (DL) models for plant diseases detection across multiple crops and controlling their actuators for the plant diseases treatment. For the plant diseases detection, the paper augments a dataset of 25,940 images to classify 11-classes of plant leaves using a pre-trained ResNet50 model, which scores the testing accuracy of 99.8%, compared to other traditional works. Experimentally, the F1-score, Recall, and Precision, for ResNet50 model were 99.91%, 99.92%, and 100%, respectively.
Why it matches plant phenotyping methods植物葉画像から病害状態を推定する深層学習モデルと、検出・処置用のIoTシステム／ハードウェアを中心的に開発・評価しているため、植物フェノタイピング手法として含める。
abstractit presents a new IoT system architecture and a hardware pilot based on the existing central pivot to develop deep learning (DL) models for plant diseases detection across multiple crops
Reproduction assets foundThe paper's plant-phenotyping measurements (ResNet50 training/validation/testing on 25,940 leaf images) are based on the public New Plant Diseases Dataset from Kaggle, explicitly stated by the authors. No author analysis code, trained model checkpoints, or supplementary data deposit is mentioned.Dataset · publicThe used dataset is available on the Kaggle repositoryOpen asset ↗Kagglepdf-page:7 lines:1-60Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
In complex farmland environments, wheat canopy coverage is insufficient at the tillering stage, posing a considerable challenge to the accurate extraction of its canopy using UAV(unmanned air vehicle) remote sensing images. In this paper, an end-to-end semantic segmentation method based on visible light (RGB) and thermal infrared (TIR) images, Tiff-SegFormer, which fused spectral features and temperature features effectively, is proposed for accurate pixel-level classification of winter wheat at the tillering stage images taken by UAV to segment the wheat canopy and background. Tiff-SegFormer utilizes hierarchical feature representation and efficient self-attention in the encoder stage to extract features of detail contours of RGB images and temperature changes of TIFF images, respectively. In the decoder stage, the features are concatenated and then the channel and spatial attention mechanisms are superimposed, aiming to further improve the segmentation accuracy and efficiency of winter wheat at the tillering stage in UAV remote sensing images. The results show that Tiff-SegFormer can achieve accurate segmentation of wheat canopy and background from UAV images of winter wheat at the tillering stage (mIoU = 84.28%, mPA = 88.97%, accuracy = 94.55%). In order to verify the efficiency of the proposed method, Tiff-SegFormer is compared with four widely used semantic segmentation methods, all of which show better performance. The four methods are UNet, DeepLabv3+, HRNet, SegFormer and four-channel (RGB + TIFF) Segformer. The generalization test shows that the proposed Tiff-SegFormer also achieves better performance than other comparison methods (mIoU = 84.94%, mPA = 91.46%, accuracy = 94.71%). Tiff-SegFormer provides a robust and efficient tool for segmenting winter wheat canopy from UAV remote sensing images of winter wheat at the tillering stage, and has great potential in applications (model implementation and results can be found at https://github.com/wylSUGAR/Tiff-SegFormer ).
Why it matches plant phenotyping methodsUAVのRGB・熱赤外画像から冬コムギのキャノピーを抽出するセマンティックセグメンテーション手法を開発し、複数手法との比較および汎化性能検証を行っており、植物状態の取得方法が中心である。
abstractan end-to-end semantic segmentation method based on visible light (RGB) and thermal infrared (TIR) images, Tiff-SegFormer, which fused spectral features and temperature features effectively, is proposed for accurate pixel-level classification of winter wheat at the tillering stage images taken by UAV to segment the wheat canopy and background.
Reproduction assets foundThe paper publicly releases the UAV RGB/TIR winter wheat tillering-stage image dataset and the TIR-to-TIFF conversion code via two author GitHub repositories. The Tiff-SegFormer model repository is referenced but its URL is not among the allowed URLs, and labelme is a generic third-party tool, so neither is included.Dataset · publicThe image can be found at
https://github.com/wylSUGAR/wheat_tillering_stage.Open asset ↗wylSUGAR/wheat_tillering_stagepdf-page:2 lines:56-74Code · publicthe TIR image was
converted into a TIFF image (the code can be found at https://github.com/wylSUGAR/TIR_DJ_tiff)Open asset ↗wylSUGAR/TIR_DJ_tiffpdf-page:2 lines:56-74Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
As one of the important indicators of soybean seed quality identification, the appearance of soybeans has always been of great concern to people, and in traditional detection, it is mainly through the naked eye to check whether there are defects on its surface. The field of machine learning, particularly deep learning technology, has undergone rapid advancements and development, making it possible to detect the defects of soybean seeds using deep learning technology. This method can effectively replace the traditional detection methods in the past and reduce the human resources consumption in this work, leading to decreased expenses associated with agricultural activities. In this paper, we propose a Yolov9-c-ghost-Forward model improved by introducing GhostConv, a lightweight convolutional module in GhostNet, which enhances the recognition of soybean seed images through grayscale conversion, filtering processing, image segmentation, morphological operations, etc. and greatly reduces the noise in them, to separate the soybean seeds from the original images. Based on the Yolov9 network, the soybean seed features are extracted, and the defects of soybean seeds are detected. Based on the experiments' findings, the recall rate can reach 98.6%, and the mAP0.5 can reach 99.2%. This shows that the model can provide a solid theoretical foundation and technical support for agricultural breeding screening and agricultural development.
Why it matches plant phenotyping methods大豆種子表面の欠陥という植物器官の状態を、画像処理と改良YOLOv9で抽出・検出する手法開発が研究の中心であり、性能評価も行っている。
abstractwe propose a Yolov9-c-ghost-Forward model improved by introducing GhostConv, a lightweight convolutional module in GhostNet, which enhances the recognition of soybean seed images through grayscale conversion, filtering processing, image segmentation, morphological operations, etc.
Reproduction assets foundThe paper's soybean seed defect detection study uses a public Kaggle dataset of 4,388 soybean seed images (intact, broken, skin-damaged, spotted), explicitly stated in the Data Availability statement. No author analysis code or trained model checkpoints are deposited; makesense.ai is a generic annotation tool, not a deDataset · publicuthors reviewed and approved the final manuscript.
Funding
This work was supported in part by the Special Support Plan for High level Talents in Zhejiang Province (2021R52019), and the Education Department of Hainan Province (Hnky2024-18).
Data availability
The data used in this article can be downloaded from the following link https://www.kaggle.com/datasets/warcoder/soyabean-seeds .
Competing interests
The authors declare no competing interests.
Footnotes
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Contributor Information
Xia Yu, Email: 100170@hainnu.edu.cn.
Qi Dai, Email: daiqi@zstu.edu.cn.
ReferenOpen asset ↗Kaggle · warcoder/soyabean-seedslines:388-411Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Cassava is a tuberous edible plant native to the American tropics and is essential for its versatile applications including cassava flour, bread, tapioca, and laundry starch. Cassava leaf diseases reduce crop yields, elevate production costs, and disrupt market stability. This places significant burdens on farmers and economies while highlighting the need for effective management strategies. Traditional methods of manual disease diagnosis are costly, labor-intensive, and time-consuming. This research aims to address the challenge of accurate disease classification by overcoming the limitations of existing methods, which encounter difficulties with the complexity and variability of leaf disease symptoms. To the best of our knowledge, this is the first study to propose a novel dual-track feature aggregation architecture that integrates the Residual Inception Positional Encoding Attention (RIPEA) Network with EfficientNet for the classification of cassava leaf diseases. The proposed model employs a dual-track feature aggregation architecture which integrates the RIPEA Network with EfficientNet. The RIPEA track extracts significant features by leveraging residual connections for preserving gradients and uses multi-scale feature fusion for combining fine-grained details with broader patterns. It also incorporates Coordinate and Mixed Attention mechanisms which focus on cross-channel and long-range dependencies. The extracted features from both tracks are aggregated for classification. Furthermore, it incorporates an image augmentation method and a cosine decay learning rate schedule to improve model training. This improves the ability of the model to accurately differentiate between Cassava Bacterial Blight (CBB), Brown Streak Disease (CBSD), Green Mottle (CGM), Mosaic Disease (CMD), and healthy leaves, addressing both local textures and global structures. Additionally, to enhance the interpretability of the model, we apply Grad-CAM to provide visual explanations for the model's decision-making process, helping to understand which regions of the leaf images contribute to the classification results. The proposed network achieved a classification accuracy of 93.06%.
Why it matches plant phenotyping methods葉画像から病徴・病害状態を分類する深層学習手法の開発が研究の中心であり、植物病害フェノタイピング手法に該当する。
abstractThis research aims to address the challenge of accurate disease classification
Reproduction assets foundThe paper uses the public Kaggle cassava leaf disease classification dataset (Makerere AI Lab images) as its phenotyping input, with an explicit data availability statement pointing to Kaggle. No author analysis code, trained model checkpoints, or supplementary repository is disclosed; the Kaggle URL itself is not in aDataset · publicThe datasets generated and/or analyzed during the current study are available in Kaggle.Open asset ↗Kagglepdf-page:14 lines:1-78Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Corn seed breeding is a global issue, and has attracted great attention in recent years. Deploying autonomous robots for corn kernel recognition and classification has great potential in terms of constructing environmentally friendly agriculture, and saving manpower. Existing segmentation methods that utilize U-shaped architectures typically operate by processing images in discrete pixel-based segments. This approach often overlooks the finer pixel-level structural details within these segments, leading to models that struggle to preserve the continuity of target edges effectively. In this paper, we propose a new framework for corn seed image segmentation, called VMUnet-MSADI, which aims to integrate MSADI module into the encoder and decoder of the VMUnet architecture. Our VMUnet-MSADI model benefits from self-attention computation in VMUnet and multi-scale coding to effectively model non-local dependencies and context relationships at the scale layer, thus improving the segmentation quality of different images. Unlike previous Unet-based improvement schemes, the proposed VMUnet-MSADI adopts a multiscale convolutional attention module coding mechanism at the depth level and an efficient multiscale deep convolutional decoder at the spatial level to extract coarse-grained features and fine-grained features at different semantic scales and effectively avoid the loss of information at the target boundary to improve the quality and accuracy of target segmentation. We introduce a Visual State Space (VSS) block to capture a wide range of contextual information and a Detail Infusion Block (DIB) to enhance the fusion of low-level and high-level features, which further fills in the remote contextual information during the up-sampling process. Comprehensive experiments were conducted on open-source datasets and the results demonstrate that the VMUnet-MSADI model excels in the task of corn kernel segmentation. The model achieved a segmentation accuracy of 95.96%, surpassing the leading method by 0.9%. Compared to other segmentation models, our method exhibits superior performance in both accuracy and loss metrics. Extensive comparative experiments conducted on various benchmark datasets further substantiate that our approach outperforms the state-of-the-art models. Code, pre-trained models and data processing protocols are available at https://github.com/corbining/VMUnet-MSADI .
Why it matches plant phenotyping methodsトウモロコシ種子画像から不健全カーネルを抽出する新規セグメンテーション手法の開発・ベンチマークが中心であり、画像ベースの植物器官状態の表現型取得に該当する。
abstractIn this paper, we propose a new framework for corn seed image segmentation, called VMUnet-MSADI
Reproduction assets foundThe paper reports corn kernel segmentation experiments and explicitly states that code, pre-trained models, and data processing protocols are publicly available at the authors' GitHub repository, which is a paper-specific actionable asset. The corn kernel dataset itself is described as open-source from GaoZhe Tech but,Code · publicCode, pre-trained models and data processing protocols are
available at https://github.com/corbining/VMUnet-MSADI.Open asset ↗corbining/VMUnet-MSADIpdf-page:1 lines:1-57Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Early automation in identifying plant diseases is crucial for the precise protection of crops. Plant diseases pose substantial risks to agriculture-dependent nations, often leading to notable crop losses and financial challenges, particularly in developing countries. Symptoms such as chlorosis, structural deformities, and wilting, characterize these diseases. However, early identification can be challenging due to symptoms similarity. Researchers using artificial intelligence (AI) for plant disease classification, challenges like data imbalance, symptom variability, real-time performance, and costly annotation hinder accuracy and adoption. This work introduced a novel approach using the You Only Look Once (YOLO) deep learning model, chosen for its exceptional accuracy and speed. The study focuses on analyzing YOLO models, specifically YOLOv3 and YOLOv4, to identify fruit plant diseases. This work examines healthy peach and strawberry leaves, as well as peach leaves affected by bacterial spots and strawberry leaves with scorch disease. These models underwent thorough training using data from the publicly accessible Plant Village dataset. The simulation results were highly promising, numerically YOLOv3 model achieved 97% accuracy and a Mean Average Precision (mAP) of 92%, within a total detection time of 105 s. In comparison, the YOLOv4 model outperformed, with a 98% accuracy and an impressive mean average precision of 98%, all while completing the detection process in just 29 s. YOLOv4 demonstrated lower complexity, significantly faster, and more precise performance, especially in detecting multiple items. Serving as an efficient real-time detector, it holds the potential to transform plant disease diagnosis and mitigation strategies, ultimately leading to increased agricultural productivity and enhanced financial outcomes for developing nations.
Why it matches plant phenotyping methods植物葉の病徴・病害状態を画像から識別するYOLO手法の開発と性能比較が研究の中心であり、植物病害フェノタイピング手法に該当する。
abstractThis work introduced a novel approach using the You Only Look Once (YOLO) deep learning model
Reproduction assets foundThe paper's plant-phenotyping input is the publicly available Plant Village dataset on Kaggle (4,222 peach/strawberry leaf images across four classes), used to train and evaluate YOLOv3/YOLOv4 disease-detection models. No author analysis code, trained model checkpoints, or paper-specific supplements are publicly statedDataset · publicThis study utilizes data from the publicly available Plant Village dataset 41 , accessible on Kaggle.Open asset ↗Kagglelines:139-155Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
CowpeaSoybeanRootMorphology / geometry measurementSegmentationRoot system architecture
A simple Python algorithm was used to estimate the four major root traits: total root length (TRL), surface area (SA), average diameter (AD), and root volume (RV) of legumes (adzuki bean, mung bean, cowpea, and soybean) based on two-dimensional images. Four different thresholding methods; Otsu, Gaussian adaptive, mean adaptive and triangle threshold were used to know the effect of thresholding in root trait estimation and to optimize the accuracy of root trait estimation. The results generated by the algorithm applied to 400 legume root images were compared with those generated by two separate software (WinRHIZO and RhizoVision), and the algorithm was validated using ground truth data. Distance transform method was used for estimating SA, AD, and RV and ConnectedComponentsWithStat function for TRL estimation. Among the thresholding methods, Otsu thresholding worked well for distance transform, while triangle threshold was effective for TRL. All the traits showed a high correlation with an R² ≥0.98 (p < 0.001) with the ground truth data. The root mean square error (RMSE) and mean bias error (MBE) were also minimal when comparing the algorithm-derived values to the ground truth values, with RMSE and MBE both < 10 for TRL, < 6 for SA, and < 0.5 for AD and RV. This lower value of error metrics indicates smaller differences between the algorithm-derived values and software-derived values. Although the observed error metrics were minimal for both software, the algorithm-derived root traits were closely aligned with those derived from WinRHIZO. We provided a simple Python algorithm for easy estimation of legume root traits where the images can be analyzed without any incurring expenses, and being open source; it can be modified by an expert based on their requirements.
Why it matches plant phenotyping methods根の二次元画像から主要形質を抽出するPythonアルゴリズムを開発し、既存ソフトウェアおよびグラウンドトゥルースで検証しており、植物フェノタイピング手法が研究の中心です。
abstractA simple Python algorithm was used to estimate the four major root traits: total root length (TRL), surface area (SA), average diameter (AD), and root volume (RV) of legumes
Reproduction assets foundThe authors publicly release their Python root-trait analysis source code together with the 400 legume root images and validation images on GitHub, as stated in the article text and Data availability statement. The Zenodo DOI cited for ground-truth images is a third-party dataset from Rose and Lobet (2018), i.e., citedCode · publicThe source code along with the root images and the validation images can be downloaded from ( https://github.com/AG9843/Legume-Root-Analysis.git ).Open asset ↗AG9843/Legume-Root-Analysislines:65-75Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Timely detection of plant diseases is crucial for agricultural safety, product quality, and environmental protection. However, plant disease detection faces several challenges, including the diversity of plant disease scenarios and complex backgrounds. To address these issues, we propose a plant disease detection model named PYOLO. Firstly, the model enhances feature fusion capabilities by optimizing the PAN structure, introducing a weighted bidirectional feature pyramid network (BiFPN), and repeatedly fusing top and bottom scale features. Additionally, the model's ability to focus on different parts of the image is improved by redesigning the EC2f structure and dynamically adjusting the convolutional kernel size to better capture features at various scales. Finally, the MHC2f mechanism is designed to enhance the model's ability to perceive complex backgrounds and targets at different scales by utilizing its self-attention mechanism for parallel processing. Experiments demonstrate that the model's mAP value increases by 4.1% compared to YOLOv8n, confirming its superiority in plant disease detection.
Why it matches plant phenotyping methods植物病害を画像から検出するPYOLOモデルを開発し、YOLOv8nとの性能比較で検証しているため、植物の病害状態を推定する画像ベースの表現型計測手法が中心である。
abstractwe propose a plant disease detection model named PYOLO.
Reproduction assets foundThe paper's authors publicly release the YOLOv8-based PYOLO/YOLO-ESC model code used for plant disease detection via a GitHub release, matching an allowed URL. No separate phenotype dataset or trained checkpoint is explicitly deposited in the supplied text.Code · publicThe improvements and execution process of the YOLOv8 code discussed in this article are available on GitHub, and can be downloaded from https://github.com/WANG9711/my-source-code/releases/tag/yolov8 under the file name yolov8.zip.Open asset ↗WANG9711/my-source-code · yolov8lines:193-288Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
The soybean-cyst nematode (SCN; Heterodera glycines) is one of the most destructive pests affecting soybean crops. Effective management of SCN is imperative for the sustainability of soybean agriculture. A promising approach to achieving this goal is the development and breeding of new resistant soybean varieties. Researchers and breeders typically employ exploratory methods such as Genome-Wide Association Studies or Quantitative Trait Loci mapping to identify genes linked to resistance. These methods depend on extensive phenotypic screening. The primary phenotypic measure for assessing SCN resistance is often the number of cysts that form on a plant's root system. Manual counting hundreds of cysts on a given root system is not only laborious but also subject to variability due to individual assessor differences. Additionally, while measuring cyst size could provide valuable insights due to its correlation with cyst development, this aspect is frequently overlooked because it demands even more hands-on work. To address these challenges, we have created Nemacounter, an intuitive software designed to detect, count, and measure the size of cysts autonomously. Nemacounter boasts a user-friendly graphical interface, simplifying the process for users to obtain reliable results. It enhances productivity by delivering annotated images and compiling data into csv files for easy analysis and reporting.
Why it matches plant phenotyping methodsダイズ根上の線虫シスト数とサイズという植物病害抵抗性関連形質を、画像から自動検出・計測するソフトウェアを開発しており、表現型取得手法が研究の中心です。
abstractwe have created Nemacounter, an intuitive software designed to detect, count, and measure the size of cysts autonomously.
Reproduction assets foundThe paper's SCN cyst phenotyping assets are publicly available: the authors' Nemacounter analysis software on GitHub, two annotated cyst image datasets on Roboflow (bounding-box and segmentation/area annotations), and the authors' trained YOLOv5-xl model (cystmodel.pt) on Iowa State's Box. The SAM weights and ultralyptCode · publicThe Nemacounter software can be downloaded here: https://github.com/DjampaKozlowski/NemaCounter and we provide an installation manual and utilization manual as supplementary data.Open asset ↗DjampaKozlowski/NemaCounterlines:65-70Dataset · publicThe complete dataset is accessible on the Roboflow website at: https://universe.roboflow.com/iowa-state-university-cwvqa/cystnewboundingboxv2Open asset ↗lines:118-138Dataset · publicAll training datasets are available on Roboflow website at : https://universe.roboflow.com/iowa-state-university-cwvqa/cystnewboundingboxv2 and https://universe.roboflow.com/iowa-state-university-cwvqa/cyst-detectors-area.Open asset ↗lines:139-197Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Assessing the health status of vegetation is of vital importance for all stakeholders. Multi-spectral and hyper-spectral imaging systems are tools for evaluating the health of vegetation in laboratory settings, and also hold the potential of assessing vegetation of large portions of land. However, the literature lacks benchmark datasets to test algorithms for predicting plant health status, with most researchers creating tailored datasets. This work presents a dataset composed of multi-spectral images, hyper-spectral reflectance values, and measurements of weight, chlorophyll, and nitrogen content of leaves at five different drying stages, from avocado, olive, and grape trees, which are common crops in the Valparaíso region of Chile. This dataset is a valuable asset for developing tools in the field of precision agriculture and assessing the general health status of vegetation.
Why it matches plant phenotyping methods植物のマルチスペクトル・ハイパースペクトル画像と葉の水分状態・化学形質を含む評価用データセットを構築しており、フェノタイピング手法開発のためのベンチマークが中心である。
abstractThis work presents a dataset composed of multi-spectral images, hyper-spectral reflectance values, and measurements of weight, chlorophyll, and nitrogen content of leaves at five different drying stages
Reproduction assets foundThe paper's multispectral images, hyperspectral reflectance, and trait measurements (weight, chlorophyll, nitrogen, fuel moisture) are publicly deposited on Figshare with an explicit DOI. The authors' sample Matlab code is included within that dataset. The MicaSense imageprocessing repository is a generic third-party工具Dataset · publicAll the data is available at this repository DOI: https://doi.org/10.6084/m9.figshare.26950660.v2.Open asset ↗figshare · 10.6084/m9.figshare.26950660.v2pdf-page:14 lines:1-35Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
Identification and diagnosis of tobacco diseases are prerequisites for the scientific prevention and control of these ailments. To address the limitations of traditional methods, such as weak generalization and sensitivity to noise in segmenting tobacco leaf lesions, this study focused on four tobacco diseases: angular leaf spot, brown spot, wildfire disease, and frog eye disease. Building upon the Unet architecture, we developed the Multi-scale Residual Dilated Segmentation Model (MD-Unet) by enhancing the feature extraction module and integrating attention mechanisms. The results demonstrated that MD-Unet achieved 92.75%, 90.94%, 84.93%, and 91.81% for the lesion CPA, recall, IoU, and F1 metrics, respectively, with an overall Dice score of 94.67%. Furthermore, the model parameters, floating-point operations, and inference time per single image for MD-Unet were 4.65 × 10 7 , 2.3392 × 10 11 , and 65.096 ms, respectively. Compared to Unet, PSP, DeepLab v3+, FCN, SegNet, UNET++, and DoubleU-Net, MD-Unet significantly improved accuracy while effectively managing model complexity, achieving optimal overall performance. This work provides the theoretical foundations and technical support for precise segmentation of tobacco lesions, with potential applications in the segmentation of other plant diseases.
Why it matches plant phenotyping methodsタバコ葉の病斑を画像から分割・定量する深層学習手法を開発し、複数モデルと精度・計算量・推論時間を比較検証しており、植物病害状態の取得方法が中心である。
abstractwe developed the Multi-scale Residual Dilated Segmentation Model (MD-Unet)
Reproduction assets foundThe paper's tobacco leaf disease image dataset is explicitly stated as publicly available via a Kaggle DOI in the Data availability statement. Labelme is a generic annotation tool, not a paper-specific asset; no author code or model checkpoint is released.Dataset · publicThe dataset was publicly available and the linkage is https://doi.org/10.34740/kaggle/dsv/10393041.Open asset ↗kaggle · 10.34740/kaggle/dsv/10393041pdf-page:15 lines:1-61Code / dataset availability confirmedEurope PMC · checked 6 Sept 2026
A wide range of portable chlorophyll meters are increasingly being used to measure leaf chlorophyll content as an indicator of plant performance, providing reference data for remote sensing studies. We tested the effect of leaf anatomy on the relationship between optical assessments of chlorophyll (Chl) against biochemically determined Chl content as a reference. Optical Chl assessments included measurements taken by four chlorophyll meters: three transmittance-based (SPAD-502, Dualex-4 Scientific, and MultispeQ 2.0), one fluorescence-based (CCM-300), and vegetation indices calculated from the 400-2500 nm leaf reflectance acquired using an ASD FieldSpec and a contact plant probe. Three leaf types with different anatomy were included: dorsiventral laminar leaves, grass leaves, and needles. On laminar leaves, all instruments performed well for chlorophyll content estimation (R 2 > 0.80, nRMSE 2 > 0.90, nRMSE 2 = 0.45, nRMSE = 11%) and failed for SPAD. For Norway spruce needles, the relation of CCM-300 values to chlorophyll content was also weak (R 2 = 0.45, nRMSE = 11%). To improve the accuracy of data used for remote sensing algorithm development, we recommend calibration of chlorophyll meter measurements with biochemical assessments, especially for species with anatomy other than laminar dicot leaves. The take-home message is that portable chlorophyll meters perform well for laminar leaves and grasses with wider leaves, however, their accuracy is limited for conifer needles and narrow grass leaves. Species-specific calibrations are necessary to account for anatomical variations, and adjustments in sampling protocols may be required to improve measurement reliability.
Why it matches plant phenotyping methods携帯型クロロフィルメーターによる葉クロロフィル量推定を、葉の解剖学的差異と生化学測定を基準に比較・検証し、校正とサンプリング改善を提案しているため、植物表現型取得法が中心です。
abstractWe tested the effect of leaf anatomy on the relationship between optical assessments of chlorophyll (Chl) against biochemically determined Chl content as a reference.
Reproduction assets foundThe paper's Data availability statement explicitly deposits the study's chlorophyll measurement and trait data in a public Zenodo repository, which is an allowed URL. No separate author analysis code URL is given (analyses were in Matlab/R), so the qualifying asset is the deposited dataset.Dataset · publicData are available in Zenodo repository found by https://zenodo.org/records/14615430.Open asset ↗Zenodo · 14615430pdf-page:14 lines:1-62Code / dataset availability confirmedOpenAlex · Europe PMC · checked 6 Sept 2026
The hairiness of the leaves is an essential morphological feature within the genus Vitis that can serve as a physical barrier. A high leaf hair density present on the abaxial surface of the grapevine leaves influences their wettability by repelling forces, thus preventing pathogen attack such as downy mildew and anthracnose. Moreover, leaf hairs as a favorable habitat may considerably affect the abundance of biological control agents. The unavailability of accurate and efficient objective tools for quantifying leaf hair density makes the study intricate and challenging. Therefore, a validated high-throughput phenotyping tool was developed and established in order to detect and quantify leaf hair using images of single grapevine leaf discs and convolution neural networks (CNN). We trained modified ResNet CNNs with a minimalistic number of images to efficiently classify the area covered by leaf hairs. This approach achieved an overall model prediction accuracy of 95.41%. As final validation, 10,120 input images from a segregating F1 biparental population were used to evaluate the algorithm performance. ResNet CNN-based phenotypic results compared to ground truth data received by two experts revealed a strong correlation with R values of 0.98 and 0.92 and root-mean-square error values of 8.20% and 14.18%, indicating that the model performance is consistent with expert evaluations and outperforms the traditional manual rating. Additional validation between expert vs. non-expert on six varieties showed that non-experts contributed to over- and underestimation of the trait, with an absolute error of 0% to 30% and -5% to -60%, respectively. Furthermore, a panel of 16 novice evaluators produced significant bias on set of varieties. Our results provide clear evidence of the need for an objective and accurate tool to quantify leaf hairiness.
Why it matches plant phenotyping methodsブドウ葉の毛密度という形態形質を画像とCNNで自動定量する高スループット手法を開発し、専門家評価および大規模集団で検証しており、表現型取得・抽出法が研究の中心である。
abstractTherefore, a validated high-throughput phenotyping tool was developed and established in order to detect and quantify leaf hair using images of single grapevine leaf discs and convolution neural networks (CNN).
Reproduction assets foundThe authors publicly released the ResNet CNN training code, the leaf disc image datasets, and the full leaf hair quantification pipeline in a GitHub repository, directly reproducing this paper's phenotyping analysis.Code · publicAll datasets and the code to train the CNNs are available in the GitHub repository.Open asset ↗lines:91-99Dataset · publicThe script of the ResNet CNN along with the images are available in the GitHub repository: https://github.com/1708nagarjun/ResNet-CNN-Leaf-hair.Open asset ↗1708nagarjun/ResNet-CNN-Leaf-hairlines:143-183Code / dataset availability confirmedCrossref · Europe PMC · checked 15 Sept 2026
Abstract The rice plant is one of the most significant crops in the world, and it suffers from various diseases. The traditional methods for rice disease detection are complex and time-consuming, mainly depending on the expert’s experience. The explosive growth in image processing, computer vision, and deep learning techniques provides effective and innovative agriculture solutions for automatically detecting and classifying these diseases. Moreover, more information can be extracted from the input images due to different feature extraction techniques. This paper proposes a new system for detecting and classifying rice plant leaf diseases by fusing different features, including color texture with Local Binary Pattern (LBP) and color features with Color Correlogram (CC). The proposed system consists of five stages. First, input images acquire RGB images of rice plants. Second, image preprocessing applies data augmentation to solve imbalanced problems, and logarithmic transformation enhancement to handle illumination problems has been applied. Third, the features extraction stage is responsible for extracting color features using CC and color texture features using multi-level multi-channel local binary pattern (MCLBP). Fourth, the feature fusion stage provides complementary and discriminative information by concatenating the two types of features. Finally, the rice image classification stage has been applied using a one-against-all support vector machine (SVM). The proposed system has been evaluated on three benchmark datasets with six classes: Blast (BL), Bacterial Leaf Blight (BLB), Brown Spot (BS), Tungro (TU), Sheath Blight (SB), and Leaf Smut (LS) have been used. Rice Leaf Diseases First Dataset, Second Dataset, and Third Dataset achieved maximum accuracy of 99.53%, 99.4%, and 99.14%, respectively, with processing time from $$100(\pm 17)ms$$ . Hence, the proposed system has achieved promising results compared to other state-of-the-art approaches.
Why it matches plant phenotyping methodsイネ葉の病徴を画像から検出・分類する特徴抽出および分類システムが研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法に該当する。
abstractThis paper proposes a new system for detecting and classifying rice plant leaf diseases by fusing different features, including color texture with Local Binary Pattern (LBP) and color features with Color Correlogram (CC).
Reproduction assets foundThe paper evaluates its rice leaf disease classification system on three publicly available image datasets, each with explicit public URLs in the Data Availability statement. No author analysis code or trained models are shared.Dataset · publichors have read and agreed to the published version of the manuscript.
Funding
Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).
Data availibility
This research study was tested using three datasets which are publicly available in: https://data.mendeley.com/datasets/fwcj7stb8r/1 . https://www.kaggle.com/datasets/rajeshbhattacharjee/rice-diseases-using-cnn-and-svm . https://data.mendeley.com/datasets/dwtn3c6w6p/1 .
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Competing interests
Te authors declare no competing interests.
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Springer Nature remains neutral with regard to jurisdictional claims in publishedOpen asset ↗fwcj7stb8rlines:2657-2679Dataset · publicof the manuscript.
Funding
Open access funding provided by The Science, Technology & Innovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).
Data availibility
This research study was tested using three datasets which are publicly available in: https://data.mendeley.com/datasets/fwcj7stb8r/1 . https://www.kaggle.com/datasets/rajeshbhattacharjee/rice-diseases-using-cnn-and-svm . https://data.mendeley.com/datasets/dwtn3c6w6p/1 .
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Competing interests
Te authors declare no competing interests.
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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
1.Open asset ↗Kaggle · rajeshbhattacharjee/rice-diseases-using-cnn-and-svmlines:2657-2679Dataset · publicInnovation Funding Authority (STDF) in cooperation with The Egyptian Knowledge Bank (EKB).
Data availibility
This research study was tested using three datasets which are publicly available in: https://data.mendeley.com/datasets/fwcj7stb8r/1 . https://www.kaggle.com/datasets/rajeshbhattacharjee/rice-diseases-using-cnn-and-svm . https://data.mendeley.com/datasets/dwtn3c6w6p/1 .
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Te authors declare no competing interests.
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Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
1. Viana, C. M., Freire, D., Abrantes, P., Rocha, J. & Pereira, P. Agricultural land syOpen asset ↗dwtn3c6w6plines:2657-2679Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
This paper presents a novel approach to agricultural disease diagnostics through the integration of Deep Learning (DL) techniques with Visual Question Answering (VQA) systems, specifically targeting the detection of wheat rust. Wheat rust is a pervasive and destructive disease that significantly impacts wheat production worldwide. Traditional diagnostic methods often require expert knowledge and time-consuming processes, making rapid and accurate detection challenging. We drafted a new, WheatRustDL2024 dataset (7998 images of healthy and infected leaves) specifically designed for VQA in the context of wheat rust detection and utilized it to retrieve the initial weights on the federated learning server. This dataset comprises high-resolution images of wheat plants, annotated with detailed questions and answers pertaining to the presence, type, and severity of rust infections. Our dataset also contains images collected from various sources and successfully highlights a wide range of conditions (different lighting, obstructions in the image, etc.) in which a wheat image may be taken, therefore making a generalized universally applicable model. The trained model was federated using Flower. Following extensive analysis, the chosen central model was ResNet. Our fine-tuned ResNet achieved an accuracy of 97.69% on the existing data. We also implemented the BLIP (Bootstrapping Language-Image Pre-training) methods that enable the model to understand complex visual and textual inputs, thereby improving the accuracy and relevance of the generated answers. The dual attention mechanism, combined with BLIP techniques, allows the model to simultaneously focus on relevant image regions and pertinent parts of the questions. We also created a custom dataset (WheatRustVQA) with our augmented dataset containing 1800 augmented images and their associated question-answer pairs. The model fetches an answer with an average BLEU score of 0.6235 on our testing partition of the dataset. This federated model is lightweight and can be seamlessly integrated into mobile phones, drones, etc. without any hardware requirement. Our results indicate that integrating deep learning with VQA for agricultural disease diagnostics not only accelerates the detection process but also reduces dependency on human experts, making it a valuable tool for farmers and agricultural professionals. This approach holds promise for broader applications in plant pathology and precision agriculture and can consequently address food security issues.
Why it matches plant phenotyping methods小麦葉の画像からさび病の有無・種類・重症度を推定するVQA、データセット、連合学習モデルを開発・評価しており、植物病害状態の取得が中心的な方法貢献である。
abstractThis dataset comprises high-resolution images of wheat plants, annotated with detailed questions and answers pertaining to the presence, type, and severity of rust infections.
Reproduction assets foundThe paper's data availability statement explicitly releases the authors' FL/VQA code on GitHub and the paper-specific wheat rust image datasets (WheatRustDL2024, WheatRustVQA images and question-answer text) via public SharePoint/Google Drive/Docs links.Dataset · public• This study introduces a Federated Learning and a Visual Question-Answering model. These models are available online on this study’s GitHub (https://github.com/aknnvt/FL-VQA-in-Wheat-Rust). • The custom datasets curated for this study, WheatRustDL2024 (https://bitspilaniac-my.sharepoint.com/:f:/g/personal/f20212378_pilani_bits-pilani_ac_in/EvwjsY_JT4FIu7ZTU8zyXOMB8Ywk4OXgO6LYwTk8dOiN_Q? e=45eTOD) and WheatRustVQA (https://docs.google.com/document/d/1EvVdrMi7W-JZkeeePEkNmVIEJ1dn7eln/edit? usp=sharing&ouid=114090611032812705334&rtpof=true&sd=true), are available for public use. Additionally, the images in WheatRustVQA (https://drive.google.com/drive/folders/1izs5ZVmi9V__ixk4ODiJAyachAulP3RL? Open asset ↗WheatRustDL2024lines:292-351Dataset · publicmodels are available online on this study’s GitHub (https://github.com/aknnvt/FL-VQA-in-Wheat-Rust). • The custom datasets curated for this study, WheatRustDL2024 (https://bitspilaniac-my.sharepoint.com/:f:/g/personal/f20212378_pilani_bits-pilani_ac_in/EvwjsY_JT4FIu7ZTU8zyXOMB8Ywk4OXgO6LYwTk8dOiN_Q? e=45eTOD) and WheatRustVQA (https://docs.google.com/document/d/1EvVdrMi7W-JZkeeePEkNmVIEJ1dn7eln/edit? usp=sharing&ouid=114090611032812705334&rtpof=true&sd=true), are available for public use. Additionally, the images in WheatRustVQA (https://drive.google.com/drive/folders/1izs5ZVmi9V__ixk4ODiJAyachAulP3RL? usp=drive_link). The raw version of the answers can be found on the GitHub repository.
DecOpen asset ↗WheatRustVQAlines:292-351Dataset · public/g/personal/f20212378_pilani_bits-pilani_ac_in/EvwjsY_JT4FIu7ZTU8zyXOMB8Ywk4OXgO6LYwTk8dOiN_Q? e=45eTOD) and WheatRustVQA (https://docs.google.com/document/d/1EvVdrMi7W-JZkeeePEkNmVIEJ1dn7eln/edit? usp=sharing&ouid=114090611032812705334&rtpof=true&sd=true), are available for public use. Additionally, the images in WheatRustVQA (https://drive.google.com/drive/folders/1izs5ZVmi9V__ixk4ODiJAyachAulP3RL? usp=drive_link). The raw version of the answers can be found on the GitHub repository.
Declarations
Competing interests
The authors declare no competing interests.
References
1.
Abebe W
Wheat Leaf Rust Disease Management: a review
J. Plant. Pathol. Microbiol. 2021 12 1 8 Abebe, W. Wheat Leaf RusOpen asset ↗WheatRustVQAlines:292-351Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Recent advancements in hyperspectral imaging (HSI) for early disease detection have shown promising results, yet there is a lack of validated high-resolution (spatial and spectral) HSI data representing the responses of plants at different stages of leaf disease progression. To address these gaps, we used bacterial leaf spot (Xanthomonas perforans) of tomato as a model system. Hyperspectral images of tomato leaves, validated against in planta pathogen populations for seven consecutive days, were analyzed to reveal differences between infected and healthy leaves. Machine learning models were trained using leaf-level full spectra data, leaf-level Vegetation index (VI) data, and pixel-level full spectra data at four disease progression stages. The results suggest that HSI can detect disease on tomato leaves at pre-symptomatic stages and differentiate bacterial disease spots from abiotic leaf spots. Using VI data as features for machine learning improved overall classification performance by 26-37% compared to the direct use of raw data. Critical wavelength bands and VIs varied across disease progression stages, suggesting that pre-symptomatic disease detection relied more on changes in leaf water content (1400 nm) and plant defense hormone-mediated responses (750 nm) rather than changes in leaf pigments or internal structure (800-900 nm), which may become more crucial during symptomatic stages. In conclusion, this study provides valuable insights into the dynamics of bacterial spot disease, revealing the potential benefits of leaf structure segmentation and VI group pattern analysis in HSI studies for the early detection of leaf diseases.
Why it matches plant phenotyping methodsトマト葉の病徴状態をハイパースペクトル画像と機械学習で推定し、病害進行段階、前症状検出、異常葉斑との識別を検証することが中心である。
abstractHyperspectral images of tomato leaves, validated against in planta pathogen populations for seven consecutive days, were analyzed to reveal differences between infected and healthy leaves.
Reproduction assets foundThe paper's raw hyperspectral image data (tomato leaf HSI used for phenotyping/analysis) are publicly deposited on Ag Data Commons. No author analysis code is publicly shared; evaluation metrics are only available upon request.Dataset · publicSpecies at Risk of Extinction and the Convention on the Trade in Endangered Species of Wild Fauna and Flora.
Author contributions
X.Z. designed and conducted the experiment. X.Z. analyzed the data. X.Z., B.V, and S.L. wrote the manuscript.
Data availability
The raw hyperspectral image data have been uploaded to Ag Data Commons: https://data.nal.usda.gov/dataset/early-detection-bacterial-spot-disease-tomato-hyperspectral-imaging . Full evaluation metrics for all models are available upon request.
Declarations
Competing interests
The authors declare no competing interests.
Footnotes
Publisher’s note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and instOpen asset ↗Ag Data Commonslines:100-122Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Identification of high carotenoid germplasm is crucial to assist breeders in provitamin-A biofortification of sorghum (Sorghum bicolor [L.] Moench). High-performance liquid chromatography is the gold standard for carotenoid quantification, however, it is not feasible for large scale phenotyping due to its high cost and low throughput. In this study, we tested the feasibility of using grain color as a high-throughput method of carotenoid biofortification breeding. We hypothesized that visual, color-based selection can be an effective strategy to identify high-carotenoid accessions. Yellow grain had significantly higher carotenoid content than red, brown, and white grain. The degree of yellowness could distinguish the presence or absence of carotenoids, but could not distinguish carotenoid concentrations within yellow-only accessions. The degree of luminosity of the grain, however, was able to better predict carotenoid concentrations within yellow-only accessions. Genome-wide association studies identified significant marker-trait associations for qualitative and quantitative grain color traits and carotenoid concentrations near carotenoid pathway genes-ZEP, PDS, CYP97A, NCED, CCD, and LycE-three of which were common between grain color and carotenoid traits. These findings suggest that using grain color as a method for screening germplasm may be an effective high-throughput selection tool for prebreeding and early-stage breeding in carotenoid biofortification.
Why it matches plant phenotyping methods穀粒色を用いたカロテノイド含量推定・高スループット選抜法の実現可能性を検証しており、植物形質取得法が研究の中心である。
abstractIn this study, we tested the feasibility of using grain color as a high-throughput method of carotenoid biofortification breeding.
Reproduction assets foundThe paper's grain-color/carotenoid phenotyping data are in public supplementary files (Supplementary Data S1–S3: GRIN color traits, visual scores, colorimeter measurements), and the authors' analysis code is publicly deposited on GitHub with an explicit availability statement.Code · publicAll other data files are available in the supplemental files and code is available at: https://github.com/rmcdower/sorghumbiofortification/tree/a8457f87068867eb687235c255a6222863102e1aOpen asset ↗rmcdower/sorghumbiofortification · a8457f87068867eb687235c255a6222863102e1alines:134-147Dataset · publicThree grains each per accession were scored independently by two individuals and classified as white, yellow, red, or brown (Supplementary Data S2).Open asset ↗lines:71-78Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Pollination is critical for crop development, especially those essential for subsistence. This study addresses the pollination challenges faced by Actinidia, a dioecious plant characterized by female and male flowers on separate plants. Despite the high protein content of pollen, the absence of nectar in kiwifruit flowers poses difficulties in attracting pollinators. Consequently, there is a growing interest in using artificial intelligence and robotic solutions to enable pollination even in unfavourable conditions. These robotic solutions must be able to accurately detect flowers and discern their genders for precise pollination operations. Specifically, upon identifying female Actinidia flowers, the robotic system should approach the stigma to release pollen, while male Actinidia flowers should target the anthers to collect pollen. We identified two primary research gaps: (1) the lack of gender-based flower detection methods and (2) the underutilisation of contemporary deep learning models in this domain. To address these gaps, we evaluated the performance of four pretrained models (YOLOv8, YOLOv5, RT-DETR and DETR) in detecting and determining the gender of Actinidia flowers. We outlined a comprehensive methodology and developed a dataset of manually annotated flowers categorized into two classes based on gender. Our evaluation utilised k-fold cross-validation to rigorously test model performance across diverse subsets of the dataset, addressing the limitations of conventional data splitting methods. DETR provided the most balanced overall performance, achieving precision, recall, F1 score and mAP of 89%, 97%, 93% and 94%, respectively, highlighting its robustness in managing complex detection tasks under varying conditions. These findings underscore the potential of deep learning models for effective gender-specific detection of Actinidia flowers, paving the way for advanced robotic pollination systems.
Why it matches plant phenotyping methodsキウイフルーツ花の画像検出と雌雄判定という植物器官の状態推定手法を開発・比較検証し、注釈付きデータセットと交差検証による性能評価も行っているため、フェノタイピング手法が中心である。
abstractwe evaluated the performance of four pretrained models (YOLOv8, YOLOv5, RT-DETR and DETR) in detecting and determining the gender of Actinidia flowers.
Reproduction assets foundThe paper's authors publicly deposited their gender-annotated Actinidia flower image dataset (augmented version) on Zenodo, explicitly linked in the Data availability statement. No author analysis code or trained model checkpoints are stated as publicly available.Dataset · publicThe data presented in this study are openly available in the digital repository Zenodo: Actinidia chinensis cv. ’Hayward’ Flower Dataset 2024 (augmented version)— https://doi.org/10.5281/zenodo.13692222Open asset ↗Zenodo · 10.5281/zenodo.13692222lines:226-255Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
This study introduces a comprehensive approach for classifying individual malting barley kernels, involving dual-sided kernel imaging, a specifically designed image processing algorithm, an optimized deep neural network architecture, and a mechanical sorting system. The proposed method achieves precise classification into multiple classes, aligning with quality standards for malting material assessment. Throughout the study, various image analysis techniques were assessed, including traditional feature engineering, established transfer learning deep neural network architectures, and our custom-designed convolutional neural network tailored for barley kernel image analysis. Comparative analysis underscores the superior performance of our network model. The study reveals that our proposed deep learning network achieves a 94% accuracy in classifying barley kernel defects and varieties, outperforming well-established transfer learning models to complex architectures that attain 93% accuracy. Additionally, it surpasses the traditional machine learning approach involving feature extraction and support vector machine classifiers, which achieve accuracy below 90% in detecting defective kernels and below 70% in varietal classification. However, we also noted the traditional approach's advantage in morphological feature recognition. This observation guides new research toward integrating morphological feature extraction techniques with modern convolutional networks. This paper presents a deep neural network designed specifically for the analysis of cereal kernel images in two applications: defect and variety classification. It emphasizes the importance of standardizing kernel orientation and merging images from both sides of the kernel, and introduces a device for image acquisition that fulfills this need.
Why it matches plant phenotyping methods麦芽大麦粒の欠陥・品種という植物器官の状態・属性を、両面画像、画像処理、深層学習、画像取得装置で分類する方法が研究の中心であり、比較検証も行っている。
abstractThis study introduces a comprehensive approach for classifying individual malting barley kernels, involving dual-sided kernel imaging, a specifically designed image processing algorithm, an optimized deep neural network architecture, and a mechanical sorting system.
Reproduction assets foundThe paper's dual-sided malting barley kernel image dataset (MaBaKI) is publicly deposited in a repository with an explicit DOI, matching an allowed URL. No author analysis code or trained model checkpoints are stated as publicly available.Dataset · publicThe datasets generated and/or analysed during the current study are available in the Malting Barley Kernel Images (MaBaKI) database repository. The MaBaKI dataset is available at https://doi.org/10.34658/RDB.MMLNNX.Open asset ↗MaBaKI · 10.34658/RDB.MMLNNXlines:170-191Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Huanglongbing (HLB), associated with the psyllid-vectored phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), is a disease threat to all citrus production worldwide. Currently, there are no sustainable curative or prophylactic treatments available. In this study, we utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues to explore how these chemistries change in vivo in HLB-infected trees. We demonstrate how spatial information from molecular maps of branches and single leaves yields insight into the biology not accessible otherwise. In particular, we found evidence that flavonoid biosynthesis is disrupted in HLB-infected trees, and an increase in the polyamine, feruloylputrescine, is highly correlated with an increase in disease severity. Based on mechanistic details revealed by these molecular maps, followed by metabolic modeling, we formulated and tested the hypothesis that CLas infection either directly or indirectly converts the precursor compound, ferulic acid, to feruloylputrescine to suppress the antimicrobial effects of ferulic acid and biosynthetically downstream flavonoids. Using in vitro bioassays, we demonstrated that ferulic acid and bioflavonoids are indeed highly bactericidal to CLas, with the activity on par with a reference antibiotic, oxytetracycline, recently approved for HLB management. We propose these compounds should be evaluated as therapeutics alternatives to the antibiotics for HLB treatment. Overall, the utilized 3D metabolic mapping approach provides a promising methodological framework to identify pathogen-specific inhibitory compounds in planta for potential prophylactic or therapeutic applications.
Why it matches plant phenotyping methods植物組織内の化学状態を3D分子マッピングで可視化し、HLB感染と病徴重症度に関連する状態を抽出する方法論的枠組みが研究の中心であり、単なる代謝測定ではない。
abstractwe utilized mass spectrometry (MS)-based metabolomics in combination with 3D molecular mapping to visualize complex chemistries within plant tissues
Reproduction assets foundThe paper deposits its citrus LC-MS/MS metabolomics raw data in MassIVE and provides GNPS molecular networking job links for its 2D/3D molecular mapping analyses. These are paper-specific, publicly accessible assets directly reproducing the study's measurements and computational analysis.Dataset · publicThe data were deposited in the MassIVE online repository and are available below links: https://massive.ucsd.edu/ProteoSAFe/dataset.jsp?task=bc1261c22e6c49d1b4f6490c7414845bOpen asset ↗MassIVE · bc1261c22e6c49d1b4f6490c7414845blines:146-218Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Nitrogen (N)-fixing symbiosis is critical to terrestrial ecosystems, yet possession of this trait is known for few plant species. Broader presence of the symbiosis is often indirectly determined by phylogenetic relatedness to taxa investigated via manipulative experiments. This data gap may ultimately underestimate phylogenetic, spatial, and temporal variation in N-fixing symbiosis. Still needed are simpler field or collections-based approaches for inferring symbiotic status. N-fixing plants differ from non-N-fixing plants in elemental and isotopic composition, but previous investigations have not tested predictive accuracy using such proxies. Here we develop a regional field study and demonstrate a simple classification model for fixer status using nitrogen and carbon content measurements, and stable isotope ratios (δ 15 N and δ 13 C), from field-collected leaves. We used mixed models and classification approaches to demonstrate that N-fixing phenotypes can be used to predict symbiotic status; the best model required all predictors and was 80-94% accurate. Predictions were robust to environmental context variation, but we identified significant variation due to native vs. non-native (exotic) status and phylogenetic affinity. Surprisingly, N content-not δ 15 N-was the strongest predictor, suggesting that future efforts combine elemental and isotopic information. These results are valuable for understudied taxa and ecosystems, potentially allowing higher-throughput field-based N-fixer assessments.
Why it matches plant phenotyping methods葉の元素・安定同位体測定と分類モデルを組み合わせ、N固定という植物状態を推定する方法を開発・精度検証しており、単なる生物学的測定ではない。
abstractHere we develop a regional field study and demonstrate a simple classification model for fixer status using nitrogen and carbon content measurements, and stable isotope ratios (δ 15 N and δ 13 C), from field-collected leaves.
Reproduction assets foundThe paper's Data availability statement explicitly deposits raw isotope/elemental phenotype data and analysis scripts in a public GitHub repository with a Zenodo stable release, including the isotope CSV dataset and phylogenetic tree used in the analyses.Code · publicRaw data and analysis scripts are available via GitHub at https://github.com/ryanafolk/isotope . A stable release of this repository is available at: https://doi.org/10.5281/zenodo.8407949Open asset ↗ryanafolk/isotopelines:143-208Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Plant diseases pose significant threats to agriculture, impacting both food safety and public health. Traditional plant disease detection systems are typically limited to recognizing disease categories included in the training dataset, rendering them ineffective against new disease types. Although out-of-distribution (OOD) detection methods have been proposed to address this issue, the impact of fine-tuning paradigms on these methods has been overlooked. This paper focuses on studying the impact of fine-tuning paradigms on the performance of detecting unknown plant diseases. Currently, fine-tuning on visual tasks is mainly divided into visual-based models and visual-language-based models. We first discuss the limitations of large-scale visual language models in this task: textual prompts are difficult to design. To avoid the side effects of textual prompts, we futher explore the effectiveness of purely visual pre-trained models for OOD detection in plant disease tasks. Specifically, we employed five publicly accessible datasets to establish benchmarks for open-set recognition, OOD detection, and few-shot learning in plant disease recognition. Additionally, we comprehensively compared various OOD detection methods, fine-tuning paradigms, and factors affecting OOD detection performance, such as sample quantity. The results show that visual prompt tuning outperforms fully fine-tuning and linear probe tuning in out-of-distribution detection performance, especially in the few-shot scenarios. Notably, the max-logit-based on visual prompt tuning achieves an AUROC score of 94.8 % in the 8-shot setting, which is nearly comparable to the method of fully fine-tuning on the full dataset (95.2 % ), which implies that an appropriate fine-tuning paradigm can directly improve OOD detection performance. Finally, we visualized the prediction distributions of different OOD detection methods and discussed the selection of thresholds. Overall, this work lays the foundation for unknown plant disease recognition, providing strong support for the security and reliability of plant disease recognition systems. We will release our code at https://github.com/JiuqingDong/PDOOD to further advance this field.
Why it matches plant phenotyping methods植物画像から未知の病害状態を認識するOOD検出手法を、複数データセットでベンチマーク・比較しており、病害表現型の取得・推定手法が研究の中心です。
abstractwe employed five publicly accessible datasets to establish benchmarks for open-set recognition, OOD detection, and few-shot learning in plant disease recognition.
Reproduction assets foundThe paper's authors state they will release their analysis code (fine-tuning paradigms, OOD detection benchmarks, and detailed results) at a public GitHub repository, and note that more detailed results are available in that code repository. The datasets used (Cotton, Mango, Strawberry, Tomato, Plant Village) are citedCode · publicWe will release our code at https://github.com/JiuqingDong/PDOOD to further
advance this field.Open asset ↗JiuqingDong/PDOODpdf-page:1 lines:1-62Code / dataset availability confirmedOpenAlex · Europe PMC · checked 15 Sept 2026
Abstract Determination of pasting properties of high quality cassava flour using rapid visco analyzer is expensive and time consuming. The use of mobile near infrared spectroscopy (SCiO™) is an alternative high throughput phenotyping technology for predicting pasting properties of high quality cassava flour traits. However, model development and validation are necessary to verify that reasonable expectations are established for the accuracy of a prediction model. In the context of an ongoing breeding effort, we investigated the use of an inexpensive, portable spectrometer that only records a portion (740–1070 nm) of the whole NIR spectrum to predict cassava pasting properties. Three machine-learning models, namely glmnet, lm, and gbm, implemented in the Caret package in R statistical program, were solely evaluated. Based on calibration statistics (R 2 , RMSE and MAE), we found that model calibrations using glmnet provided the best model for breakdown viscosity, peak viscosity and pasting temperature. The glmnet model using the first derivative, peak viscosity had calibration and validation accuracy of R 2 = 0.56 and R 2 = 0.51 respectively while breakdown had calibration and validation accuracy of R 2 = 0.66 and R 2 = 0.66 respectively. We also found out that stacking of pre-treatments with Moving Average, Savitzky Golay, First Derivative, Second derivative and Standard Normal variate using glmnet model resulted in calibration and validation accuracy of R 2 = 0.65 and R 2 = 0.64 respectively for pasting temperature. The developed calibration model predicted the pasting properties of HQCF with sufficient accuracy for screening purposes. Therefore, SCiO™ can be reliably deployed in screening early-generation breeding materials for pasting properties.
Why it matches plant phenotyping methods携帯型近赤外分光法と機械学習モデルを用いて、カッサバ育種材料のペースト特性を推定するモデルを開発・検証しており、形質取得法が研究の中心である。
abstractThe use of mobile near infrared spectroscopy (SCiO™) is an alternative high throughput phenotyping technology for predicting pasting properties of high quality cassava flour traits.
Reproduction assets foundThe paper's Data Availability statement points to a public GitHub repository containing the SCiO calibration data (spectra and pasting-property reference values) used in this study. The URL matches an allowed URL verbatim. No separate analysis code or trained model deposit is stated.Dataset · publicM.A; methodology, M.A, and W.A; data analyses, M.A and W.A; writing – original draft preparation, M.A; review and editing, P.W, E.M, G.M, E.K, R.E, P.T, S.K, I.R, P.O.O, and H.K, All authors have read and agreed to the published version of the manuscript.
Data availability
The data used in this study are available on GitHub at https://github.com/mikidadio/SCiO-Calibration-data.gi.
Competing interests
The authors declare no competing interests.
Footnotes
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
1. Hershberger, J. et al. Low-cost, handheld near-infrared spectroscopy for root dry matter conOpen asset ↗SCiO-Calibration-datalines:341-371Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 7 Sept 2026
Artificially extracted agricultural phenotype information exhibits high subjectivity and low accuracy, while the utilization of image extraction information is susceptible to interference from haze. Furthermore, the effectiveness of the agricultural image dehazing method used for extracting such information is limited due to unclear texture details and color representation in the images. To address these limitations, we propose AgriGAN (unpaired image dehazing via a cycle-consistent generative adversarial network) for enhancing the dehazing performance in agricultural plant phenotyping. The algorithm incorporates an atmospheric scattering model to improve the discriminator model and employs a whole-detail consistent discrimination approach to enhance discriminator efficiency, thereby accelerating convergence towards Nash equilibrium state within the adversarial network. Finally, by training with network adversarial loss + cycle consistent loss, clear images are obtained after dehazing process. Experimental evaluations and comparative analysis were conducted to assess this algorithm's performance, demonstrating improved accuracy in dehazing agricultural images while preserving detailed texture information and mitigating color deviation issues.
Why it matches plant phenotyping methods農業植物フェノタイピング画像から特徴情報を抽出するための画像デヘイズ手法を開発し、性能比較・評価しており、フェノタイプ取得前処理が研究の中心である。
abstractwe propose AgriGAN (unpaired image dehazing via a cycle-consistent generative adversarial network) for enhancing the dehazing performance in agricultural plant phenotyping.
Reproduction assets foundThe paper's Data availability statement explicitly commits the authors' cucumber hazy/haze-free image dataset and AgriGAN analysis code to a public GitHub repository (HZSUZJ/DLDF), which is listed in the allowed URLs. The dataset (407 haze-free / 479 hazy training images, 51/49 test images) and TensorFlow code are the纸Code · publicOur dataset and code will be publicly available at https://github.com/HZSUZJ/DLDF.Open asset ↗pdf-page:10 lines:1-65Code / dataset availability confirmedCrossref · Europe PMC · checked 7 Sept 2026
Abstract Deep learning has emerged as a highly effective and precise method for classifying images. The presence of plant diseases poses a significant threat to food security. However, accurately identifying these diseases in plants is challenging due to limited infrastructure and techniques. Fortunately, the recent advancements in deep learning within the field of computer vision have opened up new possibilities for diagnosing plant pathology. Detecting plant diseases at an early stage is crucial, and this research paper proposes a deep convolutional neural network model that can rapidly and accurately identify plant diseases. Given the minimal variation in image texture and color, deep learning techniques are essential for robust recognition. In this study, we introduce a deep, explainable neural architecture specifically designed for recognizing plant diseases. Fine-tuned deep convolutional neural network is designed by freezing the layers and adjusting the weights of learnable layers. By extracting deep features from a down sampled feature map of a fine-tuned neural network, we are able to classify these features using a customized K-Nearest Neighbors Algorithm. To train and validate our model, we utilize the largest standard plant village dataset, which consists of 38 classes. To evaluate the performance of our proposed system, we estimate specificity, sensitivity, accuracy, and AUC. The results demonstrate that our system achieves an impressive maximum validation accuracy of 99.95% and an AUC of 1, making it the most ideal and highest-performing approach compared to current state-of-the-art deep learning methods for automatically identifying plant diseases.
Why it matches plant phenotyping methods植物病害画像から病害状態を推定する深層学習手法の開発と性能評価が研究の中心であり、植物フェノタイピング手法に該当する。
abstractthis research paper proposes a deep convolutional neural network model that can rapidly and accurately identify plant diseases.
Reproduction assets foundThe paper's Data availability statement names the PlantVillage leaf-image dataset (the paper's phenotyping input) as publicly available on Kaggle; no author code or model deposit is provided.Dataset · publicThis work is based on the plant village dataset. It is publicly available at https://www.kaggle.com/datasets/abdalOpen asset ↗Kagglepdf-page:12 lines:1-86Code / dataset availability confirmedCrossref · Europe PMC · checked 14 Sept 2026
Growth chamberRGB / grayscaleRootObject detectionSegmentationGrowth / time-series analysisBiomass / plant weightGrowth / development / phenologyRoot system architecture
Abstract Flatbed scanners are commonly used for root analysis, but typical manual segmentation methods are time-consuming and prone to errors, especially in large-scale, multi-plant studies. Furthermore, the complex nature of root structures combined with noisy backgrounds in images complicates automated analysis. Addressing these challenges, this article introduces RhizoNet, a deep learning-based workflow to semantically segment plant root scans. Utilizing a sophisticated Residual U-Net architecture, RhizoNet enhances prediction accuracy and employs a convex hull operation for delineation of the primary root component. Its main objective is to accurately segment root biomass and monitor its growth over time. RhizoNet processes color scans of plants grown in a hydroponic system known as EcoFAB, subjected to specific nutritional treatments. The root detection model using RhizoNet demonstrates strong generalization in the validation tests of all experiments despite variable treatments. The main contributions are the standardization of root segmentation and phenotyping, systematic and accelerated analysis of thousands of images, significantly aiding in the precise assessment of root growth dynamics under varying plant conditions, and offering a path toward self-driving labs.
Why it matches plant phenotyping methods植物根の画像分割と根バイオマス・成長の表現型抽出ワークフローを開発・検証しており、フェノタイピング手法が中心である。
abstractthis article introduces RhizoNet, a deep learning-based workflow to semantically segment plant root scans.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publicPython codes for root scans segmentation enabled by RhizoNet were created by the authors and are described in this paper. These codes will be available free of charge upon acceptance, and with open source at: https://github.com/lbl-camera/rhizonet .Open asset ↗lbl-camera/rhizonetlines:154-177Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
To address the issues of low accuracy and slow response speed in tea disease classification and identification, an improved YOLOv7 lightweight model was proposed in this study. The lightweight MobileNeXt was used as the backbone network to reduce computational load and enhance efficiency. Additionally, a dual-layer routing attention mechanism was introduced to enhance the model's ability to capture crucial details and textures in disease images, thereby improving accuracy. The SIoU loss function was employed to mitigate missed and erroneous judgments, resulting in improved recognition amidst complex image backgrounds.The revised model achieved precision, recall, and average precision of 93.5%, 89.9%, and 92.1%, respectively, representing increases of 4.5%, 1.9%, and 2.6% over the original model. Furthermore, the model's volum was reduced by 24.69M, the total param was reduced by 12.88M, while detection speed was increased by 24.41 frames per second. This enhanced model efficiently and accurately identifies tea disease types, offering the benefits of lower parameter count and faster detection, thereby establishing a robust foundation for tea disease monitoring and prevention efforts.
Why it matches plant phenotyping methods茶葉の病害画像から病状を推定するYOLOv7改良モデルの開発・性能評価が中心であり、植物の病害状態を対象とする画像ベース表現型計測に該当する。
abstractan improved YOLOv7 lightweight model was proposed in this study
Reproduction assets foundThe paper's data availability statement says all data and code are available on GitHub and provides an authors' public URL for the improved YOLOv7 code, which matches an allowed URL. The tea disease image dataset itself is referenced but no explicit dataset URL is supplied, so only the code asset qualifies.Code · publicAll data generated or analysed during this study are available in the Github repository. Links to the code and datasets are provided in the below hyperlinked text. Code of Improved YOLOv7 project: https://github.com/anqi99/yolov7.gitOpen asset ↗https://github.com/anqi99/yolov7.gitlines:192-263Code / dataset availability confirmedOpenAlex · Europe PMC · checked 7 Sept 2026
The presence of Arbuscular Mycorrhizal Fungi (AMF) in vascular land plant roots is one of the most ancient of symbioses supporting nitrogen and phosphorus exchange for photosynthetically derived carbon. Here we provide a multi-scale modeling approach to predict AMF colonization of a worldwide crop from a Recombinant Inbred Line (RIL) population derived from Sorghum bicolor and S. propinquum. The high-throughput phenotyping methods of fungal structures here rely on a Mask Region-based Convolutional Neural Network (Mask R-CNN) in computer vision for pixel-wise fungal structure segmentations and mixed linear models to explore the relations of AMF colonization, root niche, and fungal structure allocation. Models proposed capture over 95% of the variation in AMF colonization as a function of root niche and relative abundance of fungal structures in each plant. Arbuscule allocation is a significant predictor of AMF colonization among sibling plants. Arbuscules and extraradical hyphae implicated in nutrient exchange predict highest AMF colonization in the top root section. Our work demonstrates that deep learning can be used by the community for the high-throughput phenotyping of AMF in plant roots. Mixed linear modeling provides a framework for testing hypotheses about AMF colonization phenotypes as a function of root niche and fungal structure allocations.
Why it matches plant phenotyping methods根内AMF構造をMask R-CNNで画素単位に分割し、AMF定着を高スループット推定する画像解析手法が研究の中心である。
abstractThe high-throughput phenotyping methods of fungal structures here rely on a Mask Region-based Convolutional Neural Network (Mask R-CNN) in computer vision for pixel-wise fungal structure segmentations
Reproduction assets foundThe paper's authors publicly release their segmentation/analysis code and summary data on GitHub, and the paper uses the public Cambridge AMF image dataset from Zenodo as training data. The >20,000 raw Georgia images are only available upon request.Code · publicCodes are available in GitHub: https://github.com/Arnold-Lab/image_seg_sorghum_am .Open asset ↗Arnold-Lab/image_seg_sorghum_amlines:222-293Dataset · publicThe publicly available Cambridge dataset (zenodo ID https://doi.org/10.5281/zenodo.5118948 ) included 15 whole slide scanning imagesOpen asset ↗10.5281/zenodo.5118948lines:185-204Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Agriculture plays a pivotal role in the economic development of a nation, but, growth of agriculture is affected badly by the many factors one such is plant diseases. Early stage prediction of these disease is crucial role for global health and even for game changers the farmer's life. Recently, adoption of modern technologies, such as the Internet of Things (IoT) and deep learning concepts has given the brighter light of inventing the intelligent machines to predict the plant diseases before it is deep-rooted in the farmlands. But, precise prediction of plant diseases is a complex job due to the presence of noise, changes in the intensities, similar resemblance between healthy and diseased plants and finally dimension of plant leaves. To tackle this problem, high-accurate and intelligently tuned deep learning algorithms are mandatorily needed. In this research article, novel ensemble of Swin transformers and residual convolutional networks are proposed. Swin transformers (ST) are hierarchical structures with linearly scalable computing complexity that offer performance and flexibility at various scales. In order to extract the best deep key-point features, the Swin transformers and residual networks has been combined, followed by Feed forward networks for better prediction. Extended experimentation is conducted using Plant Village Kaggle datasets, and performance metrics, including accuracy, precision, recall, specificity, and F1-rating, are evaluated and analysed. Existing structure along with FCN-8s, CED-Net, SegNet, DeepLabv3, Dense nets, and Central nets are used to demonstrate the superiority of the suggested version. The experimental results show that in terms of accuracy, precision, recall, and F1-rating, the introduced version shown better performances than the other state-of-art hybrid learning models.
Why it matches plant phenotyping methods植物葉の病害状態を画像から認識する深層学習手法の開発・比較評価が研究の中心であり、植物病害表現型の画像ベース推定に該当する。
abstractnovel ensemble of Swin transformers and residual convolutional networks are proposed.
Reproduction assets foundThe paper's plant-phenotyping measurements are based on the public PlantVillage leaf-image dataset, which the authors explicitly state is publicly available with a link in the Data availability section. No author analysis code, trained model checkpoints, or paper-specific supplements are disclosed.Dataset · publicData availability
The PlantVillage data set is available at the following link: https://github.com/spMohanty/PlantVillage-Dataset/Open asset ↗pdf-page:12 lines:1-89Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Wheat head detection and counting using deep learning techniques has gained considerable attention in precision agriculture applications such as wheat growth monitoring, yield estimation, and resource allocation. However, the accurate detection of small and dense wheat heads remains challenging due to the inherent variations in their size, orientation, appearance, aspect ratios, density, and the complexity of imaging conditions. To address these challenges, we propose a novel approach called the Oriented Feature Pyramid Network (OFPN) that focuses on detecting rotated wheat heads by utilizing oriented bounding boxes. In order to facilitate the development and evaluation of our proposed method, we introduce a novel dataset named the Rotated Global Wheat Head Dataset (RGWHD). This dataset is constructed by manually annotating images from the Global Wheat Head Detection (GWHD) dataset with oriented bounding boxes. Furthermore, we incorporate a Path-aggregation and Balanced Feature Pyramid Network into our architecture to effectively extract both semantic and positional information from the input images. This is achieved by leveraging feature fusion techniques at multiple scales, enhancing the detection capabilities for small wheat heads. To improve the localization and detection accuracy of dense and overlapping wheat heads, we employ the Soft-NMS algorithm to filter the proposed bounding boxes. Experimental results indicate the superior performance of the OFPN model, achieving a remarkable mean average precision of 85.77% in oriented wheat head detection, surpassing six other state-of-the-art models. Moreover, we observe a substantial improvement in the accuracy of wheat head counting, with an accuracy of 93.97%. This represents an increase of 3.12% compared to the Faster R-CNN method. Both qualitative and quantitative results demonstrate the effectiveness of the proposed OFPN model in accurately localizing and counting wheat heads within various challenging scenarios.
Why it matches plant phenotyping methods小麦穂の検出・計数という植物器官形質の画像ベース推定手法を開発し、専用データセットを構築して性能評価しているため、方法が中心的である。
abstractwe propose a novel approach called the Oriented Feature Pyramid Network (OFPN) that focuses on detecting rotated wheat heads by utilizing oriented bounding boxes.
Reproduction assets foundThe paper introduces the RGWHD dataset (oriented-bounding-box annotations of GWHD wheat images), publicly released via Baidu pan with extraction code, and makes its experiment scripts publicly available on GitHub. The underlying GWHD image dataset (public on Kaggle) is the image source used for the paper's phenotyping.Dataset · publiction, Validation, Writing—original draft preparation. N.L.: Formal analysis, Resources, Project ad-ministration.C .F.: Investigation, Data curation. All authors have read and agreed to the published version of the manuscript.
Data availability
The datasets generated and analysed during the current study are available in RGWHD ( https://pan.baidu.com/s/1Fy3HpIfAeQhRef_ZuKu4iw ) and the extraction code is vbiy. The datasets generated during and/or analyzed during the current study areavailable from the corresponding author on reasonable request. The scripts to run all experiments are publicly available through our GitHub page https://github.com/cwr0821/OFPN .
Competing interests
The authors deOpen asset ↗RGWHDlines:445-520Code · publicdy are available in RGWHD ( https://pan.baidu.com/s/1Fy3HpIfAeQhRef_ZuKu4iw ) and the extraction code is vbiy. The datasets generated during and/or analyzed during the current study areavailable from the corresponding author on reasonable request. The scripts to run all experiments are publicly available through our GitHub page https://github.com/cwr0821/OFPN .
Competing interests
The authors declare no competing interests.
Footnotes
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
1. Sharma, S., Kooner, R., Arora, R., Insect pests and crop losses. Breeding insect resistant crops for suOpen asset ↗cwr0821/OFPNlines:445-520Dataset · public. The GWHD dataset is a comprehensive collection of well-annotated wheat head images, compiled by nine research institutions across seven countries. It serves as a valuable resource for training robust models to accurately estimate the location and density of wheat heads in seven categories. The GWHD dataset can be accessed at https://www.kaggle.com/competitions/global-wheat-detection/data . The SPIKE dataset comprises 335 images captured at three distinct growth stages, covering ten different wheat varieties. The UWHD dataset consists of 550 images captured by a drone at an altitude of 10 m. The ACID dataset consists of 520 images taken in controlled greenhouse conditions, featuring 4158 laOpen asset ↗lines:52-149Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
A large number of countries worldwide depend on the agriculture, as agriculture can assist in reducing poverty, raising the country's income, and improving the food security. However, the plan diseases usually affect food crops and hence play a significant role in the annual yield and economic losses in the agricultural sector. In general, plant diseases have historically been identified by humans using their eyes, where this approach is often inexact, time-consuming, and exhausting. Recently, the employment of machine learning and deep learning approaches have significantly improved the classification and recognition accuracy for several applications. Despite the CNN models offer high accuracy for plant disease detection and classification, however, the limited available data for training the CNN model affects seriously the classification accuracy. Therefore, in this paper, we designed a Cycle Generative Adversarial Network (CycleGAN) to overcome the limitations of over-fitting and the limited size of the available datasets. In addition, we developed an efficient plant disease classification approach, where we adopt the CycleGAN architecture in order to enhance the classification accuracy. The obtained results showed an average enhancement of 7% in the classification accuracy.
Why it matches plant phenotyping methods植物病害を画像から分類するCycleGANベースの手法開発が研究の中心であり、植物の病害状態を直接推定するため、植物フェノタイピング手法として適格です。
abstractTherefore, in this paper, we designed a Cycle Generative Adversarial Network (CycleGAN) to overcome the limitations of over-fitting and the limited size of the available datasets.
Reproduction assets foundThe paper's Data availability statement explicitly points to the DiaMOS pear disease image dataset (the plant image dataset used for all classification and CycleGAN experiments) hosted publicly on Zenodo. No author analysis code, trained models, or generated CycleGAN image dataset is reported as publicly available.Dataset · publicData availability
The dataset that has been used in this study is available in https://zenodo.org/record/5557313.Open asset ↗zenodopdf-page:10 lines:1-64Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
This study introduces YOLOv8n-vegetable, a model designed to address challenges related to imprecise detection of vegetable diseases in greenhouse plant environment using existing network models. The model incorporates several improvements and optimizations to enhance its effectiveness. Firstly, a novel C2fGhost module replaces partial C2f. with GhostConv based on Ghost lightweight convolution, reducing the model's parameters and improving detection performance. Second, the Occlusion Perception Attention Module (OAM) is integrated into the Neck section to better preserve feature information after fusion, enhancing vegetable disease detection in greenhouse settings. To address challenges associated with detecting small-sized objects and the depletion of semantic knowledge due to varying scales, an additional layer for detecting small-sized objects is included. This layer improves the amalgamation of extensive and basic semantic knowledge, thereby enhancing overall detection accuracy. Finally, the HIoU boundary loss function is introduced, leading to improved convergence speed and regression accuracy. These improvement strategies were validated through experiments using a self-built vegetable disease detection dataset in a greenhouse environment. Multiple experimental comparisons have demonstrated the model's effectiveness, achieving the objectives of improving detection speed while maintaining accuracy and real-time detection capability. According to experimental findings, the enhanced model exhibited a 6.46% rise in mean average precision (mAP) over the original model on the self-built vegetable disease detection dataset under greenhouse conditions. Additionally, the parameter quantity and model size decreased by 0.16G and 0.21 MB, respectively. The proposed model demonstrates significant advancements over the original algorithm and exhibits strong competitiveness when compared with other advanced object detection models. The lightweight and fast detection of vegetable diseases offered by the proposed model presents promising applications in vegetable disease detection tasks.
Why it matches plant phenotyping methods温室内の野菜病害を画像から検出・推定するYOLOv8改良法を開発し、専用データセットで性能検証しており、植物の病害状態の取得が中心的な方法貢献である。
abstractThis study introduces YOLOv8n-vegetable, a model designed to address challenges related to imprecise detection of vegetable diseases in greenhouse plant environment using existing network models.
Reproduction assets foundThe paper's self-built greenhouse vegetable disease detection dataset is partially made publicly available via the authors' GitHub repository; the complete dataset and code are only available by emailing the corresponding author.Dataset · publicThe data utilized in this paper is obtained through self-gathering and is made publicly available (a part of it) to make the study reproducible. It can be accessed at https://github.com/tyuiouio/plant-disease-detection-in-real-field . If you want to request the complete dataset and code, please email the corresponding author.Open asset ↗tyuiouio/plant-disease-detection-in-real-fieldlines:243-270Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Diets consisting of greater quantity/diversity of phytochemicals are correlated with reduced risk of disease. This understanding guides policy development increasing awareness of the importance of consuming fruits, grains, and vegetables. Enacted policies presume uniform concentrations of phytochemicals across crop varieties regardless of production/harvesting methods. A growing body of research suggests that concentrations of phytochemicals can fluctuate within crop varieties. Improved awareness of how cropping practices influence phytochemical concentrations are required, guiding policy development improving human health. Reliable, inexpensive laboratory equipment represents one of several barriers limiting further study of the complex interactions influencing crop phytochemical accumulation. Addressing this limitation our study validated the capacity of a low-cost Reflectometer ($500) to measure phytochemical content in selected crops, against a commercial grade laboratory spectrophotometer. Our correlation results ranged from r 2 = 0.81 for protein in wheat and oats to r 2 = 0.99 for polyphenol content in lettuce in both the Reflectometer and laboratory spectrophotometer assessment, suggesting the Reflectometer provides an accurate accounting of phytochemical content within evaluated crops. Repeatability evaluation demonstrated good reproducibility of the Reflectometer to assess crop phytochemical content. Additionally, we confirmed large variation in phytochemical content within specific crop varieties, suggesting that cultivar is but one of multiple drivers of phytochemical accumulation. Our findings indicate dramatic nutrient variations could exist across the food supply, a point whose implications are not well understood. Future studies should investigate the interactions between crop phytochemical accumulation and farm management practices that influence specific soil characteristics.
Why it matches plant phenotyping methods作物の植物化学成分量を測定する低コスト反射計を、実験室用分光光度計と比較して精度・再現性検証しており、植物形質の取得手法の技術的検証が中心である。
abstractour study validated the capacity of a low-cost Reflectometer ($500) to measure phytochemical content in selected crops, against a commercial grade laboratory spectrophotometer.
Reproduction assets foundThe paper explicitly states that all Bionutrient Institute data (reflectometer/spectrometer phytochemical measurements used in this study) are publicly available in the authors' GitLab repository, and the authors' data-processing pipeline code is also publicly hosted on GitLab.Dataset · publicAll data derived from the Bionutrient Institute methods are available publicly from our repository: https://gitlab.com/our-sci/bionutrient-institute/dataset . The data used in this manuscript covers samples submitted up to 7/31/2022.Open asset ↗our-sci/bionutrient-institute/datasetlines:156-212Code · publicAn automated data pipeline was built using SurveyStacks API’s to merge data from each completed survey and mongoDB scripts ( https://gitlab.com/our-sci/real-food-campaign/lab-data-review-dashboard/-/tree/main ) calculated measurement outcomes.Open asset ↗our-sci/real-food-campaign/lab-data-review-dashboardlines:132-143Code / dataset availability confirmedOpenAlex · Europe PMC · checked 15 Sept 2026
Plant image analysis is a significant tool for plant phenotyping. Image analysis has been used to assess plant trails, forecast plant growth, and offer geographical information about images. The area segmentation and counting of the leaf is a major component of plant phenotyping, which can be used to measure the growth of the plant. Therefore, this paper developed a convolutional neural network-based leaf counting model called LC-Net. The original plant image and segmented leaf parts are fed as input because the segmented leaf part provides additional information to the proposed LC-Net. The well-known SegNet model has been utilised to obtain segmented leaf parts because it outperforms four other popular Convolutional Neural Network (CNN) models, namely DeepLab V3+, Fast FCN with Pyramid Scene Parsing (PSP), U-Net, and Refine Net. The proposed LC-Net is compared to the other recent CNN-based leaf counting models over the combined Computer Vision Problems in Plant Phenotyping (CVPPP) and KOMATSUNA datasets. The subjective and numerical evaluations of the experimental results demonstrate the superiority of the LC-Net to other tested models.
Why it matches plant phenotyping methodsロゼット植物の葉数という表現型を画像から推定するCNN手法を開発し、既存モデルおよび標準データセットで比較評価しており、方法が研究の中心である。
abstractTherefore, this paper developed a convolutional neural network-based leaf counting model called LC-Net.
Reproduction assets foundThe paper's leaf-counting experiments were run on the CVPPP and KOMATSUNA plant image datasets, which the authors explicitly state are publicly available with download links. No author code or trained model is released.Dataset · publication, Investigation, Validation. L.A.: Supervision, Validation, Writing-Reviewing and Editing. A.G.: Software, Visualization, Writing-Reviewing.
Funding
Open access funding provided by Linköping University.
Data availibility
The datasets that support the findings of this study are publicly available. Link for CVPPP dataset is: http://www.plant-phenotyping.org/datasets. Link for KOMATSUNA dataset is: https://limu.ait.kyushu-u.ac.jp/ agri/komatsuna/.
Competing interests
The authors declare no competing interests.
Footnotes
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
1. Furbank RT, Tester M. Open asset ↗plant-phenotyping.org · CVPPPlines:275-305Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Habanero plant diseases can significantly reduce crop yield and quality, making early detection and treatment crucial for farmers. In this study, we discuss the creation of a modified VGG16 (MVGG16) Deep Transfer Learning (DTL) model-based smartphone app for identifying habanero plant diseases. With the help of the smartphone application, growers can quickly diagnose the health of a habanero plant by taking a photo of one of its leaves. We trained the DTL model on a dataset of labelled images of healthy and infected habanero plants and evaluated its performance on a separate test dataset. The MVGG16 DTL algorithm had an accuracy, precision, f1-score, recall and AUC of 98.79%, 97.93%, 98.44%, 98.95 and 98.63%, respectively, on the testing dataset. The MVGG16 DTL model was then integrated into a smartphone app that enables users to upload photographs, get diagnosed, and explore a history of earlier diagnoses. We tested the software on a collection of photos of habanero plant leaves and discovered that it was highly accurate at spotting infected plants. The smartphone software can boost early identification and treatment of habanero plant diseases, resulting in higher crop output and higher-quality harvests.
Why it matches plant phenotyping methods葉画像から感染状態を推定する深層学習モデルとスマートフォンアプリの開発・評価が研究の中心であり、植物病害状態の表現型計測に該当する。
abstractcreation of a modified VGG16 (MVGG16) Deep Transfer Learning (DTL) model-based smartphone app for identifying habanero plant diseases
Reproduction assets foundThe authors trained and evaluated their MVGG16 habanero disease-detection model on a public Kaggle image dataset (PlantVillage), explicitly linked in the Data availability statement. No author analysis code, trained model, or app source is publicly deposited.Dataset · publicWe gathered a dataset of habanero plant images, including 1478 images of healthy plants and 997 photos of plants with one of five common diseases: bacterial spot, anthracnose, powdery mildew, Phytophthora blight, and tomato spotted wilt virus as obtained from the Kaggle repository with link: https://www.kaggle.com/datasets/arjuntejaswi/plant-village?resource=downloadOpen asset ↗Kaggle · arjuntejaswi/plant-villagelines:82-91Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
The use of unmanned aerial vehicles (UAVs) has facilitated crop canopy monitoring, enabling yield prediction by integrating regression models. However, the application of UAV-based data to individual-level harvest weight prediction is limited by the effectiveness of obtaining individual features. In this study, we propose a method that automatically detects and extracts multitemporal individual plant features derived from UAV-based data to predict harvest weight. We acquired data from an experimental field sown with 1196 Chinese cabbage plants, using two cameras (RGB and multi-spectral) mounted on UAVs. First, we used three RGB orthomosaic images and an object detection algorithm to detect more than 95% of the individual plants. Next, we used feature selection methods and five different multi-temporal resolutions to predict individual plant weights, achieving a coefficient of determination (R 2 ) of 0.86 and a root mean square error (RMSE) of 436 g/plant. Furthermore, we achieved predictions with an R 2 greater than 0.72 and an RMSE less than 560 g/plant up to 53 days prior to harvest. These results demonstrate the feasibility of accurately predicting individual Chinese cabbage harvest weight using UAV-based data and the efficacy of utilizing multi-temporal features to predict plant weight more than one month prior to harvest.
Why it matches plant phenotyping methodsUAV画像から個体特徴を自動抽出し、収穫重量という植物形質を予測する手法が研究の中心であるため。
abstractwe propose a method that automatically detects and extracts multitemporal individual plant features derived from UAV-based data to predict harvest weight.
Reproduction assets foundThe paper's analysis code is publicly available on GitHub with explicit availability language. The UAV imagery (RGB/multispectral orthomosaics and point cloud data) is only available upon reasonable request from the corresponding author, so it does not qualify as a public asset.Code · publicAll code associated with the current study is available at: https://github.com/anaguilarar/CC_Weight_Prediction .Open asset ↗anaguilarar/CC_Weight_Predictionlines:155-233Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
The world's population is expected to exceed 9 billion people by 2050, necessitating a 70% increase in agricultural output and food production to meet the demand. Due to resource shortages, climate change, the COVID-19 pandemic, and highly harsh socioeconomic predictions, such a demand is challenging to complete without using computation and forecasting methods. Machine learning has grown with big data and high-performance computers technologies to open up new data-intensive scientific opportunities in the multidisciplinary agri-technology area. Throughout the plant's developmental period, diseases and pests are natural disasters, from seed production to seedling growth. This paper introduces an early diagnosis framework for plant diseases based on fog computing and edge environment by IoT sensors measurements and communication technologies. The effectiveness of employing pre-trained CNN architectures as feature extractors in identifying plant illnesses has been studied. As feature extractors, standard pre-trained CNN models, AlexNet are employed. The obtained in-depth features are eliminated by proposing a revised version of the grey wolf optimization (GWO) algorithm that approved its efficiency through experiments. The features subset selected were used to train the SVM classifier. Ten datasets for different plants are utilized to assess the proposed model. According to the findings, the proposed model achieved better outcomes for all used datasets. As an average for all datasets, the accuracy of the proposed model is 93.84 compared to 85.49, 87.89, 87.04 for AlexNet, GoogleNet, and the SVM, respectively.
Why it matches plant phenotyping methods植物の病害を観察データから識別するCNN・GWO・SVMベースの早期診断フレームワークを提案・評価しており、植物病害状態の推定手法が中心である。
abstractThis paper introduces an early diagnosis framework for plant diseases based on fog computing and edge environment by IoT sensors measurements and communication technologies.
Reproduction assets foundThe paper's plant disease classification experiments use a public Mendeley Data leaf-image dataset covering ten plant species (healthy vs. diseased), explicitly cited in the Data availability statement with an open-access URL matching the allowed list. No author analysis code or trained model checkpoints are deposited.Dataset · publicvironmental Protection Agency
TN
True negatives
FN
False negatives
FP
False positives
TP
True positives
ACO
Ant colony optimization
PSO
Particle swarm optimization
BOA
Butterfly optimization algorithm
Author contributions
All authors have equal contributions.
Data availability
The datasets analyzed for this study available in “ https://data.mendeley.com/datasets/hb74ynkjcn/1 ” focus on plants that contribute both ecologically and economically. All datasets used are open access data, and we didn’t use any private data. Our research complies with institutional, national, and international guidelines and legislation. We have permissions from our institutional committee for scientific research eOpen asset ↗hb74ynkjcn/1lines:897-1011Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Arbuscular mycorrhizas (AM) are one of the most widespread symbiosis on earth. This plant-fungus interaction involves around 72% of plant species, including most crops. AM symbiosis improves plant nutrition and tolerance to biotic and abiotic stresses. The fungus, in turn, receives carbon compounds derived from the plant photosynthetic process, such as sugars and lipids. Most studies investigating AM and their applications in agriculture requires a precise quantification of the intensity of plant colonization. At present, the majority of researchers in the field base AM quantification analyses on manual visual methods, prone to operator errors and limited reproducibility. Here we propose a novel semi-automated approach to quantify AM fungal root colonization based on digital image analysis comparing three methods: (i) manual quantification (ii) image thresholding, (iii) machine learning. We recognize machine learning as a very promising tool for accelerating, simplifying and standardizing critical steps in analysing AM quantification, answering to an urgent need by the scientific community studying this symbiosis.
Why it matches plant phenotyping methods植物根の菌根菌コロニー形成という植物状態を、画像閾値処理と機械学習で定量する半自動手法を開発・比較しており、表現型取得・抽出法が中心である。
abstractHere we propose a novel semi-automated approach to quantify AM fungal root colonization based on digital image analysis comparing three methods: (i) manual quantification (ii) image thresholding, (iii) machine learning.
Reproduction assets foundThe paper's Data Availability statement deposits the analysed root image datasets (mycorrhizal and non-mycorrhizal) on Figshare, directly reproducing the paper's phenotyping inputs for thresholding and machine learning segmentation. The Zeiss GitHub link is a generic third-party algorithm documentation page, not a codeDataset · publicuthors have read and approved the final manuscript.
Funding
Ministero dell’Università e della Ricerca: PhD fellowship to AC; Università degli Studi di Torino: Ricerca Locale 2023 to AG.
Data availability
The analysed datasets are available from Figshare: Segmentation using thresholding and machine learning of mycorrhizal roots. https://doi.org/10.6084/m9.figshare.14679729 . Segmentation using thresholding and machine learning of non mycorrhizal roots. https://doi.org/10.6084/m9.figshare.14679684 .
Competing interests
The authors declare no competing interests.
Footnotes
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional aOpen asset ↗Figshare · 10.6084/m9.figshare.14679729lines:300-319Dataset · publicniversità degli Studi di Torino: Ricerca Locale 2023 to AG.
Data availability
The analysed datasets are available from Figshare: Segmentation using thresholding and machine learning of mycorrhizal roots. https://doi.org/10.6084/m9.figshare.14679729 . Segmentation using thresholding and machine learning of non mycorrhizal roots. https://doi.org/10.6084/m9.figshare.14679684 .
Competing interests
The authors declare no competing interests.
Footnotes
Publisher's note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
References
1. Rich MK, Nouri E, Courty PE, Reinhardt D. Diet of arbuscular mycorrhizal fungi: bread and butter? TOpen asset ↗Figshare · 10.6084/m9.figshare.14679684lines:300-319Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Conventional forest inventories are labour-intensive. This limits the spatial extent and temporal frequency at which woody vegetation is usually monitored. Remote sensing provides cost-effective solutions that enable extensive spatial coverage and high sampling frequency. Recent studies indicate that convolutional neural networks (CNNs) can classify woody forests, plantations, and urban vegetation at the species level using consumer-grade unmanned aerial vehicle (UAV) imagery. However, whether such an approach is feasible in species-rich savanna ecosystems remains unclear. Here, we tested whether small data sets of high-resolution RGB orthomosaics suffice to train U-Net, FC-DenseNet, and DeepLabv3 + in semantic segmentation of savanna tree species. We trained these models on an 18-ha training area and explored whether models could be transferred across space and time. These models could recognise trees in adjacent (mean F1-Score = 0.68) and distant areas (mean F1-Score = 0.61) alike. Over time, a change in plant morphology resulted in a decrease of model accuracy. Our results show that CNN-based tree mapping using consumer-grade UAV imagery is possible in savanna ecosystems. Still, larger and more heterogeneous data sets can further improve model robustness to capture variation in plant morphology across time and space.
Why it matches plant phenotyping methodsUAV画像とCNNによる樹木の空間的な認識・セグメンテーション手法を開発・評価し、植物形態の時空間変動に対する頑健性も検証しているため、手法が中心である。
abstractwe tested whether small data sets of high-resolution RGB orthomosaics suffice to train U-Net, FC-DenseNet, and DeepLabv3 + in semantic segmentation of savanna tree species.
Reproduction assets foundThe paper's authors explicitly state that all code used for model training and statistical analyses is publicly available on GitHub at the authors' repository (LELENet), which matches an allowed URL. This is the paper-specific analysis code for the CNN semantic segmentation of savanna tree species. No separate public影像Code · publicCode availability
All code used during model training and statistical analyses is accessible at: https://github.com/ManuelPopp/LELENet .Open asset ↗ManuelPopp/LELENetlines:136-196Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Some plant diseases can significantly reduce harvest, but their early detection in cultivation may prevent those consequential losses. Conventional methods of diagnosing plant diseases are based on visual observation of crops, but the symptoms of various diseases may be similar. It increases the difficulty of this task even for an experienced farmer and requires detailed examination based on invasive methods conducted in laboratory settings by qualified personnel. Therefore, modern agronomy requires the development of non-destructive crop diagnosis methods to accelerate the process of detecting plant infections with various pathogens. This research pathway is followed in this paper, and an approach for classifying selected Solanum lycopersicum diseases (anthracnose, bacterial speck, early blight, late blight and septoria leaf) from hyperspectral data captured on consecutive days post inoculation (DPI) is presented. The objective of that approach was to develop a technique for detecting infection in less than seven days after inoculation. The dataset used in this study included hyperspectral measurements of plants of two cultivars of S. lycopersicum: Benito and Polfast, which were infected with five different pathogens. Hyperspectral reflectance measurements were performed using a high-spectral-resolution field spectroradiometer (350-2500 nm range) and they were acquired for 63 days after inoculation, with particular emphasis put on the first 17 day-by-day measurements. Due to a significant data imbalance and low representation of measurements on some days, the collective datasets were elaborated by combining measurements from several days. The experimental results showed that machine learning techniques can offer accurate classification, and they indicated the practical utility of our approaches.
Why it matches plant phenotyping methodsトマト感染株の病害状態をハイパースペクトル測定と機械学習で非破壊・早期推定する方法を開発しており、表現型取得・判定手法が中心である。
abstractan approach for classifying selected Solanum lycopersicum diseases (anthracnose, bacterial speck, early blight, late blight and septoria leaf) from hyperspectral data captured on consecutive days post inoculation (DPI) is presented.
Reproduction assets foundThe paper's Data availability statement explicitly provides the authors' hyperspectral tomato disease measurements (the paper-specific phenotyping dataset) at a public link.Dataset · publicData availability
The hyperspectral measurements presented in this study are available at https://bit.ly/3W7VroF .Open asset ↗lines:175-230Code / dataset availability confirmedEurope PMC · checked 7 Sept 2026
Accurately detecting disease occurrences of crops in early stage is essential for quality and yield of crops through the decision of an appropriate treatments. However, detection of disease needs specialized knowledge and long-term experiences in plant pathology. Thus, an automated system for disease detecting in crops will play an important role in agriculture by constructing early detection system of disease. To develop this system, construction of a stepwise disease detection model using images of diseased-healthy plant pairs and a CNN algorithm consisting of five pre-trained models. The disease detection model consists of three step classification models, crop classification, disease detection, and disease classification. The 'unknown' is added into categories to generalize the model for wide application. In the validation test, the disease detection model classified crops and disease types with high accuracy (97.09%). The low accuracy of non-model crops was improved by adding these crops to the training dataset implicating expendability of the model. Our model has the potential to apply to smart farming of Solanaceae crops and will be widely used by adding more various crops as training dataset.
Why it matches plant phenotyping methods植物画像から病害の有無・種類を推定するCNNモデルの構築と検証が研究の中心であり、植物病害状態を直接評価する画像ベース表現型計測に該当する。
abstractconstruction of a stepwise disease detection model using images of diseased-healthy plant pairs and a CNN algorithm consisting of five pre-trained models.
Reproduction assets foundThe paper's plant disease detection models were trained on leaf image datasets explicitly released publicly: the PlantVillage dataset (GitHub) and the AI-hub strawberry field image dataset. Both are cited in the Data availability statement with exact URLs. No author analysis code or trained model checkpoints are statedDataset · publicThe PlantVillage dataset is available at https://github.com/spMohanty/PlantVillage-DatasetOpen asset ↗spMohanty/PlantVillage-Datasetlines:131-158Code / dataset availability confirmedEurope PMC · Crossref · OpenAlex · checked 7 Sept 2026
Conventional crop height measurements performed using aerial drone images require 3D reconstruction results of several aerial images obtained through structure from motion. Therefore, they require extensive computation time and their measurement accuracy is not high; if the 3D reconstruction result fails, several aerial photos must be captured again. To overcome these challenges, this study proposes a high-precision measurement method that uses a drone equipped with a monocular camera and real-time kinematic global navigation satellite system (RTK-GNSS) for real-time processing. This method performs high-precision stereo matching based on long-baseline lengths (approximately 1 m) during the flight by linking the RTK-GNSS and aerial image capture points. As the baseline length of a typical stereo camera is fixed, once the camera is calibrated on the ground, it does not need to be calibrated again during the flight. However, the proposed system requires quick calibration in flight because the baseline length is not fixed. A new calibration method that is based on zero-mean normalized cross-correlation and two stages least square method, is proposed to further improve the accuracy and stereo matching speed. The proposed method was compared with two conventional methods in natural world environments. It was observed that error rates reduced by 62.2% and 69.4%, for flight altitudes between 10 and 20 m respectively. Moreover, a depth resolution of 1.6 mm and reduction of 44.4% and 63.0% in the error rates were achieved at an altitude of 4.1 m, and the execution time was 88 ms for images with a size of 5472 × 3468 pixels, which is sufficiently fast for real-time measurement.
Why it matches plant phenotyping methodsドローン画像とRTK-GNSSを用いた植物高のリアルタイム測定法を開発し、既存法との精度・処理時間比較で検証しており、植物表現型取得が研究の中心である。
abstractA new calibration method that is based on zero-mean normalized cross-correlation and two stages least square method, is proposed to further improve the accuracy and stereo matching speed.
Reproduction assets foundThe paper's Data availability statement points to a public supplementary materials zip on the authors' site (nobuharaken.com) containing the datasets (drone images with GNSS data) generated and analysed in this plant-height measurement study. No separate analysis code repository is stated.Dataset · publicThe datasets generated and/or analysed during the current study are available in the https://nobuharaken.com/Open asset ↗nobuharaken.compdf-page:14 lines:1-60Code / dataset availability confirmedEurope PMC · Crossref · checked 7 Sept 2026
Field / plotRootWhole plant / canopy / plot / fieldObject detectionSegmentationRoot system architecture
Plant roots influence many ecological and biogeochemical processes, such as carbon, water and nutrient cycling. Because of difficult accessibility, knowledge on plant root growth dynamics in field conditions, however, is fragmentary at best. Minirhizotrons, i.e. transparent tubes placed in the substrate into which specialized cameras or circular scanners are inserted, facilitate the capture of high-resolution images of root dynamics at the soil-tube interface with little to no disturbance after the initial installation. Their use, especially in field studies with multiple species and heterogeneous substrates, though, is limited by the amount of work that subsequent manual tracing of roots in the images requires. Furthermore, the reproducibility and objectivity of manual root detection is questionable. Here, we use a Convolutional Neural Network (CNN) for the automatic detection of roots in minirhizotron images and compare the performance of our RootDetector with human analysts with different levels of expertise. Our minirhizotron data come from various wetlands on organic soils, i.e. highly heterogeneous substrates consisting of dead plant material, often times mainly roots, in various degrees of decomposition. This may be seen as one of the most challenging soil types for root segmentation in minirhizotron images. RootDetector showed a high capability to correctly segment root pixels in minirhizotron images from field observations (F1 = 0.6044; r 2 compared to a human expert = 0.99). Reproducibility among humans, however, depended strongly on expertise level, with novices showing drastic variation among individual analysts and annotating on average more than 13-times higher root length/cm 2 per image compared to expert analysts. CNNs such as RootDetector provide a reliable and efficient method for the detection of roots and root length in minirhizotron images even from challenging field conditions. Analyses with RootDetector thus save resources, are reproducible and objective, and are as accurate as manual analyses performed by human experts.
Why it matches plant phenotyping methodsCNNによるミニライゾトロン画像からの根の自動検出・根長推定を開発し、人間専門家との性能比較と再現性評価を行っており、植物表現型取得手法が研究の中心である。
abstractHere, we use a Convolutional Neural Network (CNN) for the automatic detection of roots in minirhizotron images and compare the performance of our RootDetector with human analysts with different levels of expertise.
Reproduction assets foundThe paper's authors publicly released the RootDetector CNN analysis code on GitHub, explicitly stated in the Data availability section. No separate public image/phenotype dataset deposit is stated; the minirhizotron images themselves are not explicitly deposited.Code · publicRootDetector is supplied as readily usable
code on GitHub, enabling easy use by ecologists without the need of advanced programming skills.Open asset ↗pdf-page:8 lines:1-75Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Achieving accurate and reliable maize disease identification in complex environments is a huge challenge. This is because disease images obtained from natural environments are often in complex contexts that may contain elements similar to disease characteristics or symptoms. Based on cascade network and two-stage transformation learning, the new method is proposed in this paper and applied the improved method to the task of identification and classification of four maize leaf types in a complex environment. The proposed method has a cascade structure which consists of a Faster R-CNN leaf detector (denoted as LS-RCNN) and a CNN disease classifier, named CENet(Complex Environment Network). The LS-RCNN detector with an attention mechanism was used to detect maize leaves from the image, and the CENet model further classified the leaf images detected in the first stage into four categories: Cercospora leaf spot, Common rust, Northern Leaf Blight, and Healthy, which allowed image features to be extracted more efficiently. The subsequent use of a two-stage transfer learning strategy to train CENet models of disease images in complex contexts allows for faster training of the models while ensuring accuracy. The experimental results show that the proposed method is used to identify four types of maize leaves with an F1-score of 99.70%, which is better than some popular CNN models and others' methods, and has a more obvious advantage in terms of training speed. The model proposed in this experiment has a positive significance for exploring other Crop variety identification and classification under complex backgrounds.
Why it matches plant phenotyping methods複雑環境下のトウモロコシ葉画像から病害症状を検出・分類するカスケード画像解析手法を開発し、性能評価しているため、植物病害表現型の取得が中心である。
abstractBased on cascade network and two-stage transformation learning, the new method is proposed in this paper and applied the improved method to the task of identification and classification of four maize leaf types in a complex environment.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Dataset · publicIn this experiment, corresponding datasets were created for different types of maize leaves, which can be accessed at https://github.com/gitergo/Datasets/ .Open asset ↗gitergo/Datasetslines:74-105Code / dataset availability confirmedCrossref · checked 8 Sept 2026
Abstract Mitigating the effects of global change on biodiversity requires its understanding in the past. The main proxy of plant diversity, fossil pollen record, has a complex relationship to surrounding vegetation and unknown spatial scale. We explored both using modern pollen spectra in species-rich and species-poor regions in temperate Central Europe. We also considered the biasing effects of the trees by using sites in forests and open habitats in each region. Pollen samples were collected from moss polsters at 60 sites and plant species were recorded along two 1 km-transects at each site. We found a significant positive correlation between pollen and plant richness (alpha diversity) in both complete datasets and for both subsets from open habitats. Pollen richness in forest datasets is not significantly related to floristic data due to canopy interception of pollen rather than to pollen productivity. Variances (beta diversity) of the six pollen and floristic datasets are strongly correlated. The source area of pollen richness is determined by the number of species appearing with increasing distance, which aggregates information on diversity of individual patches within the landscape mosaic and on their compositional similarity. Our results validate pollen as a reconstruction tool for plant diversity in the past.
Why it matches plant phenotyping methods現代の花粉データと植物種多様性を比較し、花粉を過去の植物多様性再構築に用いる測定・推定手法を明示的に検証しているため、単なる生態学的なルーチン測定ではない。
abstractOur results validate pollen as a reconstruction tool for plant diversity in the past.
Reproduction assets foundThe paper's pollen and vegetation datasets are deposited on Zenodo and the analysis code is on GitHub, both with explicit availability statements.Dataset · publicPollen data are available in the Neotoma Palaeoecological database. The list of the Neotoma datasets, vegetation data and further data at https://doi.org/10.5281/zenodo.7233824 .Open asset ↗zenodo · 10.5281/zenodo.7233824lines:133-178Code · publicCode to reproduce the numerical analysis is available at https://github.com/vojtechabraham/SpatialScalingPollenDiversity/ .Open asset ↗github · vojtechabraham/SpatialScalingPollenDiversitylines:133-178Code / dataset availability confirmedOpenAlex · Europe PMC · Crossref · checked 15 Sept 2026
RootAnnotation / quality controlSegmentationRoot system architecture
Accurate segmentation of root system architecture (RSA) from 2D images is an important step in studying phenotypic traits of root systems. Various approaches to image segmentation exist but many of them are not well suited to the thin and reticulated structures characteristic of root systems. The findings presented here describe an approach to RSA segmentation that takes advantage of the inherent structural properties of the root system, a segmentation network architecture we call ITErRoot. We have also generated a novel 2D root image dataset which utilizes an annotation tool developed for producing high quality ground truth segmentation of root systems. Our approach makes use of an iterative neural network architecture to leverage the thin and highly branched properties of root systems for accurate segmentation. Rigorous analysis of model properties was carried out to obtain a high-quality model for 2D root segmentation. Results show a significant improvement over other recent approaches to root segmentation. Validation results show that the model generalizes to plant species with fine and highly branched RSA's, and performs particularly well in the presence of non-root objects.
Why it matches plant phenotyping methods植物根系画像からRSAを抽出するセグメンテーション手法を開発し、データセット作成と他手法との検証・比較を行っており、植物フェノタイピング手法が中心です。
abstractAccurate segmentation of root system architecture (RSA) from 2D images is an important step in studying phenotypic traits of root systems.
Reproduction assets foundThe paper's Data availability statement provides public GitHub repositories for the authors' ITErRoot training code and the Friendly Ground Truth annotation tool used to create the paper's root segmentation ground truth. Both are paper-specific, public, and actionable. No separate phenotype image dataset deposit URL isCode · publicada First Research Excellence Fund. https://www.cfref-apogee.gc.ca/program-programme/communication_guidelines-lignes_directrices-eng.aspx . This work was also supported by the Google Cloud Platform (GCP) Research Credits Program.
Data availability
The code used to train the neural networks in this study is available on Github ( https://github.com/p2irc/ITErRoot ). The annotation tool used to create ground truth segmentations for training is available on Github ( https://github.com/p2irc/friendly_ground_truth ).
Competing interests
The authors declare no competing interests.
References
1.
Clark RT
Three-dimensional root phenotyping with a novel imaging and software platform
Plant PhysiOpen asset ↗p2irc/ITErRootlines:1379-1497Code · publicby volunteer Computer Science students with experience with other annotation tools. Friendly Ground Truth was successfully employed to generate a dataset of root images that were used to train and evaluate the segmentation network structure proposed in this work. The annotation tool has been made publicly available on GitHub ( https://github.com/p2irc/friendly_ground_truth ) for use by the community to generate root segmentation datasets.
Iterative neural network architectureOpen asset ↗p2irc/friendly_ground_truthlines:70-78Code / dataset availability confirmedEurope PMC · Crossref · checked 8 Sept 2026
Yield estimation (YE) of the crop is one of the main tasks in fruit management and marketing. Based on the results of YE, the farmers can make a better decision on the harvesting period, prevention strategies for crop disease, subsequent follow-up for cultivation practice, etc. In the current scenario, crop YE is performed manually, which has many limitations such as the requirement of experts for the bigger fields, subjective decisions and a more time-consuming process. To overcome these issues, an intelligent YE system was proposed which detects, localizes and counts the number of tomatoes in the field using SegNet with VGG19 (a deep learning-based semantic segmentation architecture). The dataset of 672 images was given as an input to the SegNet with VGG19 architecture for training. It extracts features corresponding to the tomato in each layer and detection was performed based on the feature score. The results were compared against the other semantic segmentation architectures such as U-Net and SegNet with VGG16. The proposed method performed better and unveiled reasonable results. For testing the trained model, a case study was conducted in the real tomato field at Manapparai village, Trichy, India. The proposed method portrayed the test precision, recall and F1-score values of 89.7%, 72.55% and 80.22%, respectively along with reasonable localization capability for tomatoes.
Why it matches plant phenotyping methodsトマト果実の検出・局在化・計数による収量推定手法を開発し、複数モデルと比較・実地検証しており、植物フェノタイピング手法が中心である。
abstractan intelligent YE system was proposed which detects, localizes and counts the number of tomatoes in the field using SegNet with VGG19
Reproduction assets foundThe paper's phenotyping input images come from a publicly available annotated tomato image dataset (Rob2Pheno, 123 RGB images) hosted on 4TU under CC BY 4.0, which the authors explicitly used for training their SegNet-VGG19 yield estimation model. No author analysis code or trained model is reported as publicly shared.Dataset · publicThe dataset of 123 RGB images 18 used for this work is acquired from the publicly available dataset under a creative common license. ( https://data.4tu.nl/articles/dataset/Rob2Pheno_Annotated_Tomato_Image_Dataset/13173422 ), ( https://creativecommons.org/licenses/by/4.0/ ).Open asset ↗data.4tu.nl · Rob2Pheno_Annotated_Tomato_Image_Dataset/13173422lines:74-89Code / dataset availability confirmedEurope PMC · Crossref · checked 8 Sept 2026
Plants are complex organisms subject to variable environmental conditions, which influence their physiology and phenotype dynamically. We propose to interpret plants as reservoirs in physical reservoir computing. The physical reservoir computing paradigm originates from computer science; instead of relying on Boolean circuits to perform computations, any substrate that exhibits complex non-linear and temporal dynamics can serve as a computing element. Here, we present the first application of physical reservoir computing with plants. In addition to investigating classical benchmark tasks, we show that Fragaria × ananassa (strawberry) plants can solve environmental and eco-physiological tasks using only eight leaf thickness sensors. Although the results indicate that plants are not suitable for general-purpose computation but are well-suited for eco-physiological tasks such as photosynthetic rate and transpiration rate. Having the means to investigate the information processing by plants improves quantification and understanding of integrative plant responses to dynamic changes in their environment. This first demonstration of physical reservoir computing with plants is key for transitioning towards a holistic view of phenotyping and early stress detection in precision agriculture applications since physical reservoir computing enables us to analyse plant responses in a general way: environmental changes are processed by plants to optimise their phenotype.
Why it matches plant phenotyping methods植物の葉厚センサーを用いた物理リザバーコンピューティングを提案・実証し、光合成速度や蒸散速度などの生理形質推定とストレス早期検出への応用を中心に扱うため、植物フェノタイピング手法として適格。
abstractHere, we present the first application of physical reservoir computing with plants.
Reproduction assets foundThe paper's Data availability statement explicitly deposits the datasets generated/analysed (leaf thickness sensor traces, environmental variables, gas exchange data) on Zenodo and the analysis data/code on a public GitHub repository, both with exact URLs matching allowed_urls.Dataset · publicDatasets generated and/or analysed during the current study are available in the Zenodo repository, https://doi.org/10.5281/zenodo.4264624 .Open asset ↗Zenodo · 10.5281/zenodo.4264624lines:153-237Code / dataset availability confirmedEurope PMC · checked 8 Sept 2026
Plant disease can diminish a considerable portion of the agricultural products on each farm. The main goal of this work is to provide visual information for the farmers to enable them to take the necessary preventive measures. A lightweight deep learning approach is proposed based on the Vision Transformer (ViT) for real-time automated plant disease classification. In addition to the ViT, the classical convolutional neural network (CNN) methods and the combination of CNN and ViT have been implemented for the plant disease classification. The models have been trained and evaluated on multiple datasets. Based on the comparison between the obtained results, it is concluded that although attention blocks increase the accuracy, they decelerate the prediction. Combining attention blocks with CNN blocks can compensate for the speed.
Why it matches plant phenotyping methods植物画像から病害状態を分類するVision Transformer等の手法開発・比較が研究の中心であり、植物病害フェノタイピングに該当する。
abstractA lightweight deep learning approach is proposed based on the Vision Transformer (ViT) for real-time automated plant disease classification.
Reproduction assets foundThe paper uses the public Wheat Rust Classification Dataset (Kaggle) and the authors' analysis code is publicly available on GitHub; both are paper-specific, public, and actionable.Dataset · publicThe Wheat Rust Classification Dataset is available at: https://www.kaggle.com/sinadunk23/behzad-safari-jalal .Open asset ↗lines:79-99Code · publicThe code of this paper is available at https://github.com/yasaminborhani/PlantDiseaseClassification .Open asset ↗yasaminborhani/PlantDiseaseClassificationlines:136-143Code / dataset availability confirmedEurope PMC · checked 8 Sept 2026
Common beanPepper / chilliField / plotSegmentation
Fine segmentation labelling tasks are time consuming and typically require a great deal of manual labor. This paper presents a novel method for efficiently creating pixel-level fine segmentation labelling that significantly reduces the amount of necessary human labor. The proposed method utilizes easily produced multiple and complementary coarse labels to build a complete fine label via supervised learning. The primary label among the coarse labels is the manual label, which is produced with simple contours or bounding boxes that roughly encompass an object. All others coarse labels are complementary and are generated automatically using existing algorithms. Fine labels can be rapidly created during the supervised learning of such coarse labels. In the experimental study, the proposed technique achieved a fine label IOU (intersection of union) of 92% in segmenting our newly constructed bean field dataset. The proposed method also achieved 95% and 92% mean IOU when tested on publicly available agricultural CVPPP and CWFID datasets, respectively. Our proposed method of segmentation also achieved a mean IOU of 81% when it was tested on our newly constructed paprika disease dataset, which includes multiple categories.
Why it matches plant phenotyping methods画素レベル画像セグメンテーション手法そのものを開発し、植物・農業およびパプリカ病害データセットで性能評価しているため、植物画像から状態・領域を抽出する方法研究として中心的です。
abstractThis paper presents a novel method for efficiently creating pixel-level fine segmentation labelling
Reproduction assets foundThe paper's authors explicitly state their analysis code is publicly available on GitHub. The newly constructed plant datasets (Bean-Field, Paprika-Disease, Circle) are only available on request, so they qualify as request_only, not public.Code · publicCode availability
The code is available at https://github.com/hololee/coarse-to-fine-segmentation-labelling .Open asset ↗hololee/coarse-to-fine-segmentation-labellinglines:226-263Code / dataset availability confirmedEurope PMC · checked 8 Sept 2026
We investigated prediction of malting quality (MQ) phenotypes in different locations using metabolomic spectra, and compared the prediction ability of different models, and training population (TP) sizes. Data of five MQ traits was measured on 2667 individual plots of 564 malting spring barley lines from three years and two locations. A total of 24,018 metabolomic features (MFs) were measured on each wort sample. Two statistical models were used, a metabolomic best linear unbiased prediction (MBLUP) and a partial least squares regression (PLSR). Predictive ability within location and across locations were compared using cross-validation methods. For all traits, more than 90% of the total variance in MQ traits could be explained by MFs. The prediction accuracy increased with increasing TP size and stabilized when the TP size reached 1000. The optimal number of components considered in the PLSR models was 20. The accuracy using leave-one-line-out cross-validation ranged from 0.722 to 0.865 and using leave-one-location-out cross-validation from 0.517 to 0.817. In conclusion, the prediction accuracy of metabolomic prediction of MQ traits using MFs was high and MBLUP is better than PLSR if the training population is larger than 100. The results have significant implications for practical barley breeding for malting quality.
Why it matches plant phenotyping methodsメタボロームスペクトルから大麦の麦芽品質形質を予測し、複数モデルと交差検証で予測精度を比較・検証しており、育種利用可能な形質推定法が中心です。
abstractWe investigated prediction of malting quality (MQ) phenotypes in different locations using metabolomic spectra, and compared the prediction ability of different models, and training population (TP) sizes.
Reproduction assets foundThe article states that all data used (malting quality trait records and metabolomic features for 2667 plots of 564 spring barley lines) are deposited in a public Mendeley Data repository with a direct link, making the paper's phenotyping measurements publicly available.Dataset · publicAll the data used are available in a public accessible repository with the direct link as https://data.mendeley.com/datasets/s3s4ft92wj/1 .Open asset ↗s3s4ft92wjlines:160-241Code / dataset availability confirmedOpenAlex · Europe PMC · checked 8 Sept 2026
Current methods in measuring maize (Zea mays L.) southern rust (Puccinia polyspora Underw.) and subsequent crop senescence require expert observation and are resource-intensive and prone to subjectivity. In this study, unoccupied aerial system (UAS) field-based high-throughput phenotyping (HTP) was employed to collect high-resolution aerial imagery of elite maize hybrids planted in the 2020 and 2021 growing seasons, with 13 UAS flights obtained from 2020 and 17 from 2021. In total, 36 vegetation indices (VIs) were extracted from mosaicked aerial images that served as temporal phenomic predictors for southern rust scored in the field and senescence as scored using UAS-acquired mosaic images. Temporal best linear unbiased predictors (TBLUPs) were calculated using a nested model that treated hybrid performance as nested within flights in terms of rust and senescence. All eight machine learning regressions tested (ridge, lasso, elastic net, random forest, support vector machine with radial and linear kernels, partial least squares, and k-nearest neighbors) outperformed a general linear model with both higher prediction accuracies (92-98%) and lower root mean squared error (RMSE) for rust and senescence scores (linear model RMSE ranged from 65.8 to 2396.5 across all traits, machine learning regressions RMSE ranged from 0.3 to 17.0). UAS-acquired VIs enabled the discovery of novel early quantitative phenotypic indicators of maize senescence and southern rust before being detectable by expert annotation and revealed positive correlations between grain filling time and yield (0.22 and 0.44 in 2020 and 2021), with practical implications for precision agricultural practices.
Why it matches plant phenotyping methodsUAS画像から植生指数を抽出し、機械学習でトウモロコシのさび病と老化を推定するフェノタイピング手法が研究の中心であるため。
abstractunoccupied aerial system (UAS) field-based high-throughput phenotyping (HTP) was employed to collect high-resolution aerial imagery
Reproduction assets foundThe article explicitly states that the analysis code is publicly viewable on the first author's GitHub repository, which directly reproduces the paper's machine learning phenomic prediction analysis. Supplementary Datasets S1–S3 are mentioned but no public URLs for them are provided in the supplied blocks.Code · publicThe code used in this analysis is viewable at ( https://github.com/alperadak/phenomic-prediction-/blob/main/Phenomic%20prediction ).Open asset ↗alperadak/phenomic-prediction-lines:111-127Code / dataset availability confirmedEurope PMC · checked 8 Sept 2026
ClassificationVisualization / data managementArchitecture / morphology / geometry
The significance of automatic plant identification has already been recognized by academia and industry. There were several attempts to utilize leaves and flowers for identification; however, bark also could be beneficial, especially for trees, due to its consistency throughout the seasons and its easy accessibility, even in high crown conditions. Previous studies regarding bark identification have mostly contributed quantitatively to increasing classification accuracy. However, ever since computer vision algorithms surpassed the identification ability of humans, an open question arises as to how machines successfully interpret and unravel the complicated patterns of barks. Here, we trained two convolutional neural networks (CNNs) with distinct architectures using a large-scale bark image dataset and applied class activation mapping (CAM) aggregation to investigate diagnostic keys for identifying each species. CNNs could identify the barks of 42 species with > 90% accuracy, and the overall accuracies showed a small difference between the two models. Diagnostic keys matched with salient shapes, which were also easily recognized by human eyes, and were typified as blisters, horizontal and vertical stripes, lenticels of various shapes, and vertical crevices and clefts. The two models exhibited disparate quality in the diagnostic features: the old and less complex model showed more general and well-matching patterns, while the better-performing model with much deeper layers indicated local patterns less relevant to barks. CNNs were also capable of predicting untrained species by 41.98% and 48.67% within the correct genus and family, respectively. Our methodologies and findings are potentially applicable to identify and visualize crucial traits of other plant organs.
Why it matches plant phenotyping methodsCNNとCAMを用いて樹皮画像から識別に有用な形態的特徴を抽出・可視化する方法が研究の中心であり、植物器官の観察可能な形質の推定に該当する。
abstractwe trained two convolutional neural networks (CNNs) with distinct architectures using a large-scale bark image dataset and applied class activation mapping (CAM) aggregation to investigate diagnostic keys for identifying each species.
Reproduction assets foundThe paper's own bark image dataset (BARK-KR) is publicly deposited on Zenodo, the authors' analysis scripts are on GitHub, and the CAM extended figures are hosted on Figshare. The BarkNet 1.0 dataset is cited prior work and excluded.Dataset · publicthe bark image data collected in this study were published and are available on Zenodo ( https://doi.org/10.5281/zenodo.4749062 ) 48 .Open asset ↗Zenodo · 10.5281/zenodo.4749062lines:134-164Code · publicThe python scripts used in this study are available on GitHub ( https://github.com/snutp/TBKFE ).Open asset ↗GitHub · snutp/TBKFElines:134-164Code / dataset availability confirmedCrossref · Europe PMC · checked 8 Sept 2026
Abstract Cassava brown streak disease (CBSD) is an emerging viral disease that can greatly reduce cassava productivity, while causing only mild aerial symptoms that develop late in infection. Early detection of CBSD enables better crop management and intervention. Current techniques require laboratory equipment and are labour intensive and often inaccurate. We have developed a handheld active multispectral imaging (A-MSI) device combined with machine learning for early detection of CBSD in real-time. The principal benefits of A-MSI over passive MSI and conventional camera systems are improved spectral signal-to-noise ratio and temporal repeatability. Information fusion techniques further combine spectral and spatial information to reliably identify features that distinguish healthy cassava from plants with CBSD as early as 28 days post inoculation on a susceptible and a tolerant cultivar. Application of the device has the potential to increase farmers’ access to healthy planting materials and reduce losses due to CBSD in Africa. It can also be adapted for sensing other biotic and abiotic stresses in real-world situations where plants are exposed to multiple pest, pathogen and environmental stresses.
Why it matches plant phenotyping methods植物のウイルス感染状態（病徴）を対象に、携帯型マルチスペクトル画像装置と空間・スペクトル機械学習を開発し、早期検出性能を示した研究であり、表現型取得法が中心です。
abstractWe have developed a handheld active multispectral imaging (A-MSI) device combined with machine learning for early detection of CBSD in real-time.
Reproduction assets foundThe article's Data availability statement explicitly deposits the paper's own multispectral imaging dataset (Cassava-TME204-UCBSV trials) on Zenodo. No author analysis code or trained models are stated as available.Dataset · publicThe MSI dataset of these three trials (Cassava-TME204-UCBSV) are available at https://doi.org/10.5281/zenodo.4636968 .Open asset ↗zenodo · 10.5281/zenodo.4636968lines:203-262Code / dataset availability confirmedEurope PMC · OpenAlex · Crossref · checked 13 Sept 2026
Field / plotPhotogrammetry / SfM / MVSLiDAR / point cloudRootStem / branch2D/3D reconstructionSkeletonization / topologyArchitecture / morphology / geometry
Living architecture, changing in structure with annual growth, requires precise, regular characterisation. However, its geometric irregularity and topological complexity make documentation using traditional methods difficult and presents challenges in creating useful models for mechanical and physiological analyses. Two kinds of living architecture are examined: historic living root bridges grown in Meghalaya, India, and contemporary 'Baubotanik' structures designed and grown in Germany. These structures exhibit common features, in particular network-like structures of varying complexity that result from inosculations between shoots or roots. As an answer to this modelling challenge, we present the first extensive documentation of living architecture using photogrammetry and a subsequent skeleton extraction workflow that solves two problems related to the anastomoses and varying nearby elements specific to living architecture. Photogrammetry was used as a low cost method, supplying detailed point clouds of the structures' visible surfaces. A workflow based on voxel-thinning (using deletion templates and adjusted p-simplicity criteria) provides efficient, accurate skeletons. A volume reconstruction method is derived from the thinning process. The workflow is assessed on seven characteristics beneficial in representing living architecture in comparison with alternative skeleton extraction methods. The resulting models are ready for use in analytical tools, necessary for functional, responsible design.
Why it matches plant phenotyping methods植物の生体構造をフォトグラメトリで取得し、点群から骨格・体積を再構成するワークフロー自体が中心的な方法開発であり、植物構造の表現・解析に用いるため。
abstractwe present the first extensive documentation of living architecture using photogrammetry and a subsequent skeleton extraction workflow
Reproduction assets foundThe paper's Data availability statement explicitly provides public access to the authors' skeletonisation source code on GitHub and the photogrammetric point clouds (Freiburg pavilion, Ficus joint, Baubotanik joint) on the TUM media repository. Both are paper-specific, public, and actionable.Code · publicThe source code is available at: https://github.com/QiguanShu/skeleton-abstraction-of-point-cloud-by-voxel-thinningOpen asset ↗QiguanShu/skeleton-abstraction-of-point-cloud-by-voxel-thinninglines:141-214Code / dataset availability confirmedEurope PMC · checked 9 Sept 2026
Plant functional traits ('traits') are essential for assessing biodiversity and ecosystem processes, but cumbersome to measure. To facilitate trait measurements, we test if traits can be predicted through visible morphological features by coupling heterogeneous photographs from citizen science (iNaturalist) with trait observations (TRY database) through Convolutional Neural Networks (CNN). Our results show that image features suffice to predict several traits representing the main axes of plant functioning. The accuracy is enhanced when using CNN ensembles and incorporating prior knowledge on trait plasticity and climate. Our results suggest that these models generalise across growth forms, taxa and biomes around the globe. We highlight the applicability of this approach by producing global trait maps that reflect known macroecological patterns. These findings demonstrate the potential of Big Data derived from professional and citizen science in concert with CNN as powerful tools for an efficient and automated assessment of Earth's plant functional diversity.
Why it matches plant phenotyping methods市民科学画像とCNNを用いて植物機能形質を自動推定する計算手法を開発・評価しており、形質取得が研究の中心である。
abstractwe test if traits can be predicted through visible morphological features by coupling heterogeneous photographs from citizen science (iNaturalist) with trait observations (TRY database) through Convolutional Neural Networks (CNN).
Reproduction assets foundThe paper's Data/Code availability statements provide public figshare deposits with the trained CNN ensemble models and global trait maps (10.6084/m9.figshare.13312040), raw data tables with image download links and mean trait values (10.6084/m9.figshare.14410379), the iNaturalist raw image dataset via GBIF (10.15468/3Dataset · publicthe raw data tables containing the download links for the plant images as well as the mean trait values that were the basis for further processing are available on figshare ( https://doi.org/10.6084/m9.figshare.14410379 )Open asset ↗figshare · 10.6084/m9.figshare.14410379lines:142-198Dataset · publicThe raw image dataset can be obtained from iNaturalist database via https://doi.org/10.15468/ab3s5x 47Open asset ↗10.15468/ab3s5xlines:142-198Code · publicThe code supporting this manuscript is available online at https://github.com/ChrSchiller/cnn_traitsOpen asset ↗GitHub · ChrSchiller/cnn_traitslines:142-198Code / dataset availability confirmedOpenAlex · Europe PMC · checked 8 Sept 2026
ArabidopsisLaboratory / benchtopSeed / grainObject detectionGrowth / development / phenology
A high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed with the aim of screening genotype responsiveness and chemical drugs. This technology was presently used with Arabidopsis thaliana seeds to allow characterizing seed samples germination behavior by incubating seeds in 96-well microplates under defined conditions and detecting radicle protrusion through the seed coat by automated image analysis. This study shows that this technology provides a fast procedure allowing to handle thousands of seeds without compromising repeatability or accuracy of the germination measurements. Potential biases of the experimental protocol were assessed through statistical analyses of germination kinetics. Comparison of the ScreenSeed procedure with commonly used germination tests based upon visual scoring displayed very similar germination kinetics.
Why it matches plant phenotyping methods種子発芽を自動画像解析で測定する高スループット表現型解析法を開発し、再現性・精度・既存法との一致を検証しており、測定手法自体が中心である。
abstractA high throughput phenotyping tool for seed germination, the ScreenSeed technology, was developed
Reproduction assets foundThe paper's seed-by-seed germination time measurements (the core phenotyping data) are published as online supplementary XLSX files (Supplementary Data S3, S4, S5), and a ZIP supplement (Supplementary Information 2) corresponds to the image time series (Supplementary Data S2). These are hosted with the open-access (CC Dataset · publicAll the germination time seed by seed in the analyses are provided in the Supplementary Data S3 online for Col-0 accession in water condition, in Supplementary Data S4 online for comparison with standard assays and in Supplementary Data S5 online for the analyse of Col-0 and L er ABA dose response.Open asset ↗lines:101-105Code / dataset availability confirmedEurope PMC · checked 14 Sept 2026
Enhancing nitrogen fertilization efficiency for improving yield is a major challenge for smallholder farming systems. Rapid and cost-effective methodologies with the capability to assess the effects of fertilization are required to facilitate smallholder farm management. This study compares maize leaf and canopy-based approaches for assessing N fertilization performance under different tillage, residue coverage and top-dressing conditions in Zimbabwe. Among the measurements made on individual leaves, chlorophyll readings were the best indicators for both N content in leaves (R < 0.700) and grain yield (GY) (R < 0.800). Canopy indices reported even higher correlation coefficients when assessing GY, especially those based on the measurements of the vegetation density as the green area indices (R < 0.850). Canopy measurements from both ground and aerial platforms performed very similar, but indices assessed from the UAV performed best in capturing the most relevant information from the whole plot and correlations with GY and leaf N content were slightly higher. Leaf-based measurements demonstrated utility in monitoring N leaf content, though canopy measurements outperformed the leaf readings in assessing GY parameters, while providing the additional value derived from the affordability and easiness of using a pheno-pole system or the high-throughput capacities of the UAVs.
Why it matches plant phenotyping methods葉・キャノピーのリモートセンシング手法を比較し、窒素含量や収量推定との相関を検証しており、植物表現型取得法が研究の中心である。
abstractThis study compares maize leaf and canopy-based approaches for assessing N fertilization performance under different tillage, residue coverage and top-dressing conditions in Zimbabwe.
Reproduction assets found保存済みの本文根拠を更新済みルールで再検証し、公開資産1件を確認しました。Code · publicregions of interest corresponding to each plot were segmented and exported using the MosaicTool (Shawn C. Kefauver, https://integrativecropecophysiology.com/software-development/mosaictool/ , https://gitlab.com/sckefauver/MosaicTool , University of Barcelona, Barcelona, Spain) integrated as a plugin for FIJIOpen asset ↗sckefauver/MosaicToollines:152-165Code / dataset availability confirmedEurope PMC · checked 9 Sept 2026
Optical Projection Tomography (OPT) is a powerful three-dimensional imaging technique used for the observation of millimeter-scaled biological samples, compatible with bright-field and fluorescence contrast. OPT is affected by spatially variant artifacts caused by the fact that light diffraction is not taken into account by the straight-light propagation models used for reconstruction. These artifacts hinder high-resolution imaging with OPT. In this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT, drastically reducing the amount of acquired data, compared to previously reported approaches. The method, purely based on bright-field contrast of the unstained sample, provides a comprehensive picture of the sample anatomy, as demonstrated in vivo on Arabidopsis thaliana and zebrafish embryos. Furthermore, this bright-field reconstruction can be implemented on practically any multi-view light-sheet fluorescence microscope without complex hardware modifications or calibrations, complementing the fluorescence information with tissue anatomy.
Why it matches plant phenotyping methods多視点取得と画像融合による3D明視野再構成法を開発し、Arabidopsisの解剖学的形態を実証しているため、植物形態の取得手法が中心です。
abstractIn this work we show that, by using a multiview imaging approach, a 3D reconstruction of the bright-field contrast can be obtained without the diffraction artifacts typical of OPT
Reproduction assets foundThe paper's authors state that the Python sample code implementing their bright-field multi-view reconstruction (used for the Arabidopsis thaliana and zebrafish phenotyping/imaging analysis) is publicly available on GitHub under the authors' account. The GitHub URL in the text contains formatting artifacts and does notCode · publicData processing was performed in Python; a sample code is available on GitHubOpen asset ↗pdf-page:7 lines:1-55Code / dataset availability confirmedCrossref · OpenAlex · Europe PMC · checked 9 Sept 2026
Abstract Statistical data-mining (DM) and machine learning (ML) are promising tools to assist in the analysis of complex dataset. In recent decades, in the precision of agricultural development, plant phenomics study is crucial for high-throughput phenotyping of local crop cultivars. Therefore, integrated or a new analytical approach is needed to deal with these phenomics data. We proposed a statistical framework for the analysis of phenomics data by integrating DM and ML methods. The most popular supervised ML methods; Linear Discriminant Analysis (LDA), Random Forest (RF), Support Vector Machine with linear (SVM -l ) and radial basis (SVM- r ) kernel are used for classification/prediction plant status (stress/non-stress) to validate our proposed approach. Several simulated and real plant phenotype datasets were analyzed. The results described the significant contribution of the features (selected by our proposed approach) throughout the analysis. In this study, we showed that the proposed approach removed phenotype data analysis complexity, reduced computational time of ML algorithms, and increased prediction accuracy.
Why it matches plant phenotyping methods植物フェノミクスデータを対象とした統計・機械学習解析フレームワークの提案と、シミュレーションおよび実データによる検証が中心であり、植物状態の推定手法に該当する。
abstractWe proposed a statistical framework for the analysis of phenomics data by integrating DM and ML methods.
Reproduction assets foundThe paper's real-data analysis is based on a public quantitative barley phenomics dataset downloaded from the IAP G2P site (iapg2p.sourceforge.net/modeling/#dataset), which is a paper-specific, publicly actionable asset. The authors' R analysis code is only 'available upon request', so it qualifies as a request-only,非-Dataset · publicWe downloaded the quantitative phenomics dataset from http://iapg2p.sourceforge.net/modeling/#dataset , and the details description of this dataset is available at Chen et al . 9 .Open asset ↗iapg2p.sourceforge.netlines:63-73Code / dataset availability confirmedEurope PMC · checked 15 Sept 2026
Field / plotRaman / spectroscopyRootPhysiological trait estimation
1. Root lignin is a key driver of root decomposition, which in turn is a fundamental component of the terrestrial carbon cycle and increasingly in the focus of ecologists and global climate change research. However, measuring lignin content is labor-intensive and therefore not well-suited to handle the large sample sizes of most ecological studies. To overcome this bottleneck, we explored the applicability of high-throughput near infrared spectroscopy (NIRS) measurements to predict fine root lignin content. 2. We measured fine root lignin content in 73 plots of a field biodiversity experiment containing a pool of 60 grassland species using the Acetylbromid (AcBr) method. To predict lignin content, we established NIRS calibration and prediction models based on partial least square regression (PLSR) resulting in moderate prediction accuracies (RPD = 1.96, R 2 = 0.74, RMSE = 3.79). 3. In a second step, we combined PLSR with spectral variable selection. This considerably improved model performance (RPD = 2.67, R 2 = 0.86, RMSE = 2.78) and enabled us to identify chemically meaningful wavelength regions for lignin prediction. 4. We identified 38 case studies in a literature survey and quantified median model performance parameters from these studies as a benchmark for our results. Our results show that the combination Acetylbromid extracted lignin and NIR spectroscopy is well suited for the rapid analysis of root lignin contents in herbaceous plant species even if the amount of sample is limited.
Why it matches plant phenotyping methods近赤外分光とPLSRによる植物細根リグニン含量の高速推定法を開発・検証し、性能比較とベンチマークも行っており、植物形質取得が中心である。
abstractwe explored the applicability of high-throughput near infrared spectroscopy (NIRS) measurements to predict fine root lignin content.
Reproduction assets foundThe paper's fine root lignin/NIR spectral dataset is publicly deposited in PANGAEA. The carspls, pls, baseline, and prospectr R packages are generic third-party libraries, not authors' analysis code, and no author code or trained model is deposited.Dataset · publicltivation.
Author Contributions
A.W. designed the experiment. O.E. collected the data. R.R. and O.E. analyzed the data with input of M.V. O.E., R.R. and A.W. wrote the manuscript with input from M.V. and all authors provided input on the final written manuscript.
Data Availability
The data used in this article is accessible via https://doi.pangaea.de/10.1594/PANGAEA.895501 .
Competing Interests
The authors declare no competing interests.
Footnotes
Publisher’s note: Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Oliver Elle and Ronny Richter contributed equally.
Supplementary information
Supplementary information accompanOpen asset ↗PANGAEA · 10.1594/PANGAEA.895501lines:233-256Code / dataset availability confirmedEurope PMC · checked 10 Sept 2026
Rice disease recognition is crucial in automated rice disease diagnosis systems. At present, deep convolutional neural network (CNN) is generally considered the state-of-the-art solution in image recognition. In this paper, we propose a novel rice blast recognition method based on CNN. A dataset of 2906 positive samples and 2902 negative samples is established for training and testing the CNN model. In addition, we conduct comparative experiments for qualitative and quantitatively analysis in our evaluation of the effectiveness of the proposed method. The evaluation results show that the high-level features extracted by CNN are more discriminative and effective than traditional hand-crafted features including local binary patterns histograms (LBPH) and Haar-WT (Wavelet Transform). Moreover, quantitative evaluation results indicate that CNN with Softmax and CNN with support vector machine (SVM) have similar performances, with higher accuracy, larger area under curve (AUC), and better receiver operating characteristic (ROC) curves than both LBPH plus an SVM as the classifier and Haar-WT plus an SVM as the classifier. Therefore, our CNN model is a top performing method for rice blast disease recognition and can be potentially employed in practical applications.
Why it matches plant phenotyping methodsイネ葉の画像から病害状態を認識するCNN手法の開発・比較評価が研究の中心であり、植物病害の表現型推定に該当する。
abstractwe propose a novel rice blast recognition method based on CNN.
Reproduction assets foundThe authors publicly released the rice blast disease image dataset (5808 expert-labeled 128×128 patches) used to train and test their CNN model, with an explicit availability statement and URL.Dataset · publicThe dataset is available
from the, http://www.51agritech.com/zdataset.data.zip.Open asset ↗pdf-page:2 lines:1-57Code / dataset availability confirmedEurope PMC · OpenAlex · checked 10 Sept 2026
To ensure food security in the face of population growth, decreasing water and land for agriculture, and increasing climate variability, crop yields must increase faster than the current rates. Increased yields will require implementing novel approaches in genetic discovery and breeding. Here we demonstrate the potential of field-based high throughput phenotyping (HTP) on a large recombinant population of rice to identify genetic variation underlying important traits. We find that detecting quantitative trait loci (QTL) with HTP phenotyping is as accurate and effective as traditional labor-intensive measures of flowering time, height, biomass, grain yield, and harvest index. Genetic mapping in this population, derived from a cross of an modern cultivar (IR64) with a landrace (Aswina), identified four alleles with negative effect on grain yield that are fixed in IR64, demonstrating the potential for HTP of large populations as a strategy for the second green revolution.
Why it matches plant phenotyping methods圃場ハイスループット表現型解析を大規模イネ集団に適用し、従来測定との精度・有効性を比較してQTL検出に利用しており、表現型取得法が研究の中心である。
abstractHere we demonstrate the potential of field-based high throughput phenotyping (HTP) on a large recombinant population of rice to identify genetic variation underlying important traits.
Reproduction assets foundThe paper's QTL/phenotyping analysis code is publicly hosted on Bitbucket, and the authors state that the code and original phenotyping data files are deposited in Dryad under a DOI. Both are paper-specific, public, and actionable.Code · publicR code is currently hosted at bitbucket.org ( https://bitbucket.org/paultanger/rilpopr ) and code as well as original data files will be deposited into http://dx.doi.org/10.5061/dryad.53bj8 and www.datadryad.org for publication and availability.Open asset ↗bitbucket.org/paultanger/rilpoprlines:62-105Dataset · publicR code is currently hosted at bitbucket.org ( https://bitbucket.org/paultanger/rilpopr ) and code as well as original data files will be deposited into http://dx.doi.org/10.5061/dryad.53bj8 and www.datadryad.org for publication and availability.Open asset ↗Dryad · 10.5061/dryad.53bj8lines:62-105Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 5 Sept 2026
Modern phenotyping and plant disease detection methods, based on optical sensors and information technology, provide promising approaches to plant research and precision farming. In particular, hyperspectral imaging have been found to reveal physiological and structural characteristics in plants and to allow for tracking physiological dynamics due to environmental effects. In this work, we present an approach to plant phenotyping that integrates non-invasive sensors, computer vision, as well as data mining techniques and allows for monitoring how plants respond to stress. To uncover latent hyperspectral characteristics of diseased plants reliably and in an easy-to-understand way, we "wordify" the hyperspectral images, i.e., we turn the images into a corpus of text documents. Then, we apply probabilistic topic models, a well-established natural language processing technique that identifies content and topics of documents. Based on recent regularized topic models, we demonstrate that one can track automatically the development of three foliar diseases of barley. We also present a visualization of the topics that provides plant scientists an intuitive tool for hyperspectral imaging. In short, our analysis and visualization of characteristic topics found during symptom development and disease progress reveal the hyperspectral language of plant diseases.
Why it matches plant phenotyping methods非侵襲的ハイパースペクトル画像から植物の病害進展・生理状態を抽出し、トピックモデルで自動追跡する新しい表現・解析手法が研究の中心である。
abstractwe present an approach to plant phenotyping that integrates non-invasive sensors, computer vision, as well as data mining techniques
Reproduction assets foundThe paper's authors publicly released the Python implementation of online regularized LDA used for their hyperspectral plant phenotyping analysis on GitHub. No public phenotype dataset or image deposit is stated; the hyperspectral data itself is only described, not deposited.Code · publicThe Python implementation of online regularized LDA is freely available at https://github.com/mirwaes/sclda .Open asset ↗mirwaes/scldalines:78-87