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Automatic preprocessing pipeline for individual-plant level (IPL) soybean growth monitoring through UAV multisource imagery.

Scientific Reports · 16 Jul 2026 · 10.1038/s41598-026-61673-6

Abstract

Orthoimagery derived from unmanned aerial vehicles (UAVs) has become a valuable data source for crop-growth monitoring. Individual plant-level (IPL) information enables high-throughput analyses by capturing plant-to-plant variability within fields. However, reliable IPL-based analysis requires accurate extraction of plant-specific regions, which remains challenging in soybean cultivation due to weed interference and canopy overlap. This study proposed an automatic preprocessing framework for IPL soybean growth monitoring that integrates deep-learning-based semantic segmentation with a furrow-guided region of interest (ROI) generation strategy using UAV imagery. A segmentation model was developed using combinations of RGB and multispectral orthoimagery, and a furrow line detection algorithm was designed to generate IPL ROIs aligned with crop rows. The ensemble model combining U-Net, DeepLabV3+, and SegFormer achieved the most stable performance (F1-score up to 0.94 and IoU up to 0.89). The furrow-guided ROI generation algorithm also accurately estimated crop counts, showing strong agreement with manual observations (R² = 0.90 and RMSE = 6.35). The generated IPL ROIs enabled accurate quantification of growth-related features, with strong agreement between automatically generated and manually delineated ROIs (R² > 0.90). Overall, the proposed preprocessing framework provides a practical and scalable solution for UAV-based high-throughput phenotyping in soybean and other ridge-based cropping systems.

Plant phenotyping relevance

UAV画像から個体単位の植物領域を抽出し、成長形質を定量化する前処理・セグメンテーション手法が研究の中心であるため。

abstractThis study proposed an automatic preprocessing framework for IPL soybean growth monitoring that integrates deep-learning-based semantic segmentation with a furrow-guided region of interest (ROI) generation strategy using UAV imagery.
abstractThe generated IPL ROIs enabled accurate quantification of growth-related features, with strong agreement between automatically generated and manually delineated ROIs

Code and data availability

The authors state that the complete implementation of their IPL soybean preprocessing pipeline (semantic segmentation + furrow line detection) is publicly available on Zenodo. The annotated sample dataset, however, is only available upon request from the corresponding author, so it is not a public asset.

Codepublic

The complete implementation of this pipeline is publicly available at https://doi.org/10.5281/zenodo.21095307.

Open resource ↗zenodo · 10.5281/zenodo.21095307 · pdf-page:7 lines:1-62

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