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Plant phenotyping methods.

植物形質を測っただけの研究ではなく、フェノタイピング手法の開発・検証・実質的利用・ベンチマーク・方法レビューとの関連性が見つかった論文を中心に表示します。

表示条件: Organ identification条件を解除 ×
18 papers · plant phenotyping relevance matchLatest completed run · 2016-01-01 – 2026-09-13

自動判定された未検証候補です。Catalogへの掲載にはキュレーター承認が必要です。

Plant phenotyping relevance match · UnverifiedOpenAlex · checked 14 Sept 2026
Published1 Jul 2026The Crop JournalCited by 1 · OpenAlex ↗

Lightweight contour-aware 2D Gaussian splatting under Plant-to-Camera

MaizeWheatNeRF / 3D Gaussian SplattingLiDAR / point cloudPanicle / ear / spikeLeafStem / branchMorphology / geometry measurementOrgan identificationPose / keypoint estimation

The objective of this study was to develop a 3D plant modeling strategy that enables camera pose recovery from segmented plant images and the reconstruction of an initial point cloud. A lightweight, contour-aware framework leverages the view-consistent and surface-oriented representation of 2D Gaussian Splatting, making it suitable for plant surface reconstruction under the Plant-to-Camera mode. A contour-weighted Laplacian regularization suppresses depth discontinuities around plant boundaries, while simplified Gaussian primitives improve computational efficiency without compromising geometric fidelity. Organ-level semantics are integrated into the reconstructed geometry to distinguish plant organs such as leaves, stems, and ears. On maize and wheat datasets, our method outperformed existing approaches in terms of morphological fidelity, organ-level structural consistency, and processing speed, demonstrating its suitability for plant phenotyping

Why it matches plant phenotyping methods植物器官の3D再構成と形態情報抽出を目的とする計算手法を開発し、既存法と形態忠実度・器官構造整合性・処理速度で比較評価しており、フェノタイピング手法が中心である。

abstractThe objective of this study was to develop a 3D plant modeling strategy that enables camera pose recovery from segmented plant images and the reconstruction of an initial point cloud.
Plant phenotyping relevance match · UnverifiedEurope PMC · checked 5 Sept 2026
Published3 Jun 2026Plant methodsCited by 0 · OpenAlex ↗

Developing a sinusoidal-polynomial fitting model for deriving the structural and biochemical circadian rhythms for different parts of Birch from hyperspectral LiDAR data.

LiDAR / point cloudMultispectral / hyperspectralLeafStem / branchWhole plant / canopy / plot / fieldClassificationOrgan identificationGrowth / time-series analysisArchitecture / morphology / geometryPigment / colour / senescence

A deeper understanding of circadian rhythms in plants, especially trees, is crucial for uncovering how structural and physiological processes align with daily environmental cycles. However, most studies analyze biochemical changes and positional variations separately, with limited exploration of their coordination within the whole-plant system. Hyperspectral light detection and ranging (HSL) integrates three-dimensional (3D) structural mapping with hyperspectral reflectance, enabling non-destructive assessment of plant biochemistry. Previous work showed that HSL can detect nocturnal vertical canopy displacements with centimeter accuracy, but organ-level rhythmic patterns (e.g., branches vs. leaves) remain poorly studied. Here, we developed a hyperspectral point cloud classification method combining spectral and spatial data to separate branches from leaves and analyze their sleep movements independently. A novel sinusoidal-polynomial fitting model was then proposed to characterize circadian rhythms in both sleep movements and reflectance variations. We applied HSL data from a single birch tree (Betula pendula) collected at 30 distinct HSL measurement times over 24 h to develop a 3D canopy partitioning approach that divides the canopy into nine grids (3 × 3) and ten vertical layers per grid. Results revealed near-24-hour rhythmic patterns (max R² = 0.5841, P < 0.05) and stratified sleep movements: branches exhibited larger amplitudes than the corresponding canopy layers, with the overall maximum movement amplitude occurring shortly before sunrise (04:00-06:30) and recovering after sunrise. The model also effectively characterized diurnal reflectance variations (max R² = 0.5812, P < 0.05). In addition, chlorophyll-related spectral indices exhibited a sinusoidal variation, reaching a minimum around 03:00. These findings highlight the potential of HSL for the joint analysis of structural and biochemical circadian rhythms, providing a non-destructive approach to investigate plant rhythmicity in both structural and physiological domains.

Why it matches plant phenotyping methods hyperspectral LiDARによる植物の構造・生理形質の取得と、葉・枝の分類および概日リズム推定モデルの開発が研究の中心である。

abstractHere, we developed a hyperspectral point cloud classification method combining spectral and spatial data to separate branches from leaves and analyze their sleep movements independently.
Plant phenotyping relevance match · UnverifiedCrossref · OpenAlex · checked 15 Sept 2026
Published1 Mar 2026Artificial Intelligence in AgricultureCited by 1 · OpenAlex ↗

PlantSegNeRF: A few-shot, cross-species method for plant 3D instance point cloud reconstruction via joint-channel NeRF with multi-view image instance matching

NeRF / 3D Gaussian SplattingLiDAR / point cloudRGB / grayscaleWhole plant / canopy / plot / fieldOrgan identification2D/3D reconstructionSegmentation

Organ segmentation of plant point clouds is a prerequisite for the high-resolution and accurate extraction of organ-level phenotypic traits. Although the fast development of deep learning has boosted much research on segmentation of plant point clouds, the existing techniques for organ segmentation still face limitations in resolution, segmentation accuracy, and generalizability across various plant species. In this study, we proposed a novel approach called plant segmentation neural radiance fields (PlantSegNeRF), aiming to directly generate high-precision instance point clouds from multi-view RGB image sequences for a wide range of plant species. PlantSegNeRF performed two-dimensional (2D) instance segmentation on the multi-view images to generate instance masks for each organ with a corresponding instance identification (ID). The multi-view instance IDs corresponding to the same plant organ were then matched and refined using a specially designed instance matching (IM) module. The instance NeRF was developed to render an implicit scene containing color, density, semantic and instance information, which was ultimately converted into high-precision plant instance point clouds based on volume density. The results proved that in semantic segmentation of point clouds, PlantSegNeRF outperformed the commonly used methods, demonstrating an average improvement of 16.1 %, 18.3 %, 17.8 %, and 24.2 % in precision, recall, F1-score, and intersection over union (IoU) compared to the second-best results on structurally complex datasets. More importantly, PlantSegNeRF exhibited significant advantages in instance segmentation. Across all plant datasets, it achieved average improvements of 11.7 %, 38.2 %, 32.2 % and 25.3 % in mean precision (mPrec), mean recall (mRec), mean coverage (mCov), and mean weighted coverage (mWCov), respectively. Furthermore, PlantSegNeRF demonstrates superior few-shot, cross-species performance, requiring only multi-view images of few plants to train models applicable to specific or similar varieties. This study extends organ-level plant phenotyping and provides a high-throughput way to supply high-quality 3D data for developing large-scale artificial intelligence (AI) models in plant science. • A comprehensive dataset of well-labeled two-dimensional (2D) images and point clouds dataset of plants was established, including various varieties and growth stages. 50 plant samples were collected for each type. • A novel multi-view image instance matching (IM) module was proposed to align plant organ instance identifications (IDs) across different viewpoints, serving as the foundation for organ-level instance segmentation. • A multi-channel instance neural radiance fields (NeRF) module with encoding color, semantic, and instance information was developed to achieve high-precision mapping of 2D image colors, semantics, and aligned instances into 3D space, enabling point cloud background removal and fine-grained segmentation of plant organs.

Why it matches plant phenotyping methods植物器官の3D点群再構成・インスタンス分割を開発し、セグメンテーション性能を検証する手法研究であり、器官レベル表現型抽出を直接支援するため。

abstractwe proposed a novel approach called plant segmentation neural radiance fields (PlantSegNeRF), aiming to directly generate high-precision instance point clouds from multi-view RGB image sequences for a wide range of plant species.
Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Published1 Jan 2026Methods in molecular biology (Clifton, N.J.)Cited by 0 · OpenAlex ↗

Immunolabeling Sieve Element Cell Walls with the LM26 Antibody.

Laboratory / benchtopCell / cellular structureCountingMorphology / geometry measurementOrgan identificationArchitecture / morphology / geometry

Sieve elements in the phloem transport carbon and small molecules, such as RNA and phytohormones, throughout the plant body. Understanding the physical dimensions of sieve elements and phloem tissue is thus crucial for predicting how much carbon can be moved at any given time. Quantification of sieve element diameters and areas has previously been performed using transmission electron microscopy, scanning electron microscopy, and light microscopy, but sieve element identification is difficult because the phloem is a heterogeneous tissue. The recently identified LM26 antibody labels a pectin in the sieve element cell wall, allowing the identification of sieve elements and the measurement of their properties, such as diameter, relatively quickly and the quantification of their number in cross sections using image analysis software. Here, we describe methods for immunolabelling sieve elements in fresh or fixed tissue embedded in polyethylene glycol or methacrylate. The protocol is broadly adaptable to various fixation and sectioning methods, provided they do not alter the structure of pectins in the cell wall.

Why it matches plant phenotyping methods師部篩要素の同定、画像解析による直径・面積・数の定量を可能にする免疫標識プロトコルが中心で、植物形態形質の取得手法を提供している。

abstractallowing the identification of sieve elements and the measurement of their properties, such as diameter, relatively quickly and the quantification of their number in cross sections using image analysis software.
Plant phenotyping relevance match · UnverifiedCrossref · checked 14 Sept 2026
Published4 Aug 2025Applied SciencesCited by 0 · OpenAlex ↗

Deep Point Cloud Facet Segmentation and Applications in Downsampling and Crop Organ Extraction

LiDAR / point cloudOrgan identificationCalibration / preprocessingSegmentation

To address the issues in existing 3D point cloud facet generation networks, specifically, the tendency to produce a large number of empty facets and the uncertainty in facet count, this paper proposes a novel deep learning framework for robust facet segmentation. Based on the generated facet set, two exploratory applications are further developed. First, to overcome the bottleneck where inaccurate empty-facet detection impairs the downsampling performance, a facet-abstracted downsampling method is introduced. By using a learned facet classifier to filter out and discard empty facets, retaining only non-empty surface facets, and fusing point coordinates and local features within each facet, the method achieves significant compression of point cloud data while preserving essential geometric information. Second, to solve the insufficient precision in organ segmentation within crop point clouds, a facet growth-based segmentation algorithm is designed. The network first predicts the edge scores for the facets to determine the seed facets. The facets are then iteratively expanded according to adjacent-facet similarity until a complete organ region is enclosed, thereby enhancing the accuracy of segmentation across semantic boundaries. Finally, the proposed facet segmentation network is trained and validated using a synthetic dataset. Experiments show that, compared with traditional methods, the proposed approach significantly outperforms both downsampling accuracy and instance segmentation performance. In various crop scenarios, it demonstrates excellent geometric fidelity and semantic consistency, as well as strong generalization ability and practical application potential, providing new ideas for in-depth applications of facet-level features in 3D point cloud analysis.

Why it matches plant phenotyping methods作物3D点群から器官を抽出・分割する画像解析手法を開発し、合成データで検証しており、植物形態の取得が中心的な技術貢献です。

abstractSecond, to solve the insufficient precision in organ segmentation within crop point clouds, a facet growth-based segmentation algorithm is designed.
Plant phenotyping relevance match · UnverifiedOpenAlex · arXiv · checked 8 Sept 2026
Published1 Jul 2025arXiv (Cornell University)Cited by 0 · OpenAlex ↗

PlantSegNeRF: A few-shot, cross-species method for plant 3D instance point cloud reconstruction via joint-channel NeRF with multi-view image instance matching

NeRF / 3D Gaussian SplattingLiDAR / point cloudRGB / grayscaleWhole plant / canopy / plot / fieldOrgan identification2D/3D reconstructionSegmentation

Organ segmentation of plant point clouds is a prerequisite for the high-resolution and accurate extraction of organ-level phenotypic traits. Although the fast development of deep learning has boosted much research on segmentation of plant point clouds, the existing techniques for organ segmentation still face limitations in resolution, segmentation accuracy, and generalizability across various plant species. In this study, we proposed a novel approach called plant segmentation neural radiance fields (PlantSegNeRF), aiming to directly generate high-precision instance point clouds from multi-view RGB image sequences for a wide range of plant species. PlantSegNeRF performed 2D instance segmentation on the multi-view images to generate instance masks for each organ with a corresponding ID. The multi-view instance IDs corresponding to the same plant organ were then matched and refined using a specially designed instance matching module. The instance NeRF was developed to render an implicit scene, containing color, density, semantic and instance information. The implicit scene was ultimately converted into high-precision plant instance point clouds based on the volume density. The results proved that in semantic segmentation of point clouds, PlantSegNeRF outperformed the commonly used methods, demonstrating an average improvement of 16.1%, 18.3%, 17.8%, and 24.2% in precision, recall, F1-score, and IoU compared to the second-best results on structurally complex species. More importantly, PlantSegNeRF exhibited significant advantages in plant point cloud instance segmentation tasks. Across all plant species, it achieved average improvements of 11.7%, 38.2%, 32.2% and 25.3% in mPrec, mRec, mCov, mWCov, respectively. This study extends the organ-level plant phenotyping and provides a high-throughput way to supply high-quality 3D data for the development of large-scale models in plant science.

Why it matches plant phenotyping methods植物器官のマルチビュー画像から高精度な3Dインスタンスポイントクラウドを再構成・分割する手法を開発し、植物フェノタイピングへの応用と性能比較を行っているため、方法が研究の中心です。

abstractwe proposed a novel approach called plant segmentation neural radiance fields (PlantSegNeRF), aiming to directly generate high-precision instance point clouds from multi-view RGB image sequences for a wide range of plant species.
Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 6 Sept 2026
Published22 May 2025Frontiers in Plant ScienceCited by 0 · OpenAlex ↗

Automated dynamic phenotyping of whole oilseed rape ( Brassica napus ) plants from images collected under controlled conditions.

Rapeseed / canolaLaboratory / benchtopFlowerFruitLeafStem / branchWhole plant / canopy / plot / fieldClassificationOrgan identificationGrowth / time-series analysis

Introduction Recent advancements in sensor technologies have enabled collection of many large, high-resolution plant images datasets that could be used to non-destructively explore the relationships between genetics, environment and management factors on phenotype or the physical traits exhibited by plants. The phenotype data captured in these datasets could then be integrated into models of plant development and crop yield to more accurately predict how plants may grow as a result of changing management practices and climate conditions, better ensuring future food security. However, automated methods capable of reliably and efficiently extracting meaningful measurements of individual plant components (e.g. leaves, flowers, pods) from imagery of whole plants are currently lacking. In this study, we explore interdisciplinary application of MapReader, a computer vision pipeline for annotating and classifying patches of larger images that was originally developed for semantic exploration of historical maps, to time-series images of whole oilseed rape (Brassica napus) plants. Methods Models were trained to classify five plant structures in patches derived from whole plant images (branches, leaves, pods, flower buds and flowers), as well as background patches. Three modelling methods are compared: (i) 6-label multi-class classification, (ii) a chain of binary classifiers approach, and (iii) an approach combining binary classification of plant and background patches, followed by 5-label multi-class classification of plant structures. Results A combined plant/background binarization and 5-label multi-class modelling approach using a ‘resnext50d_4s2x40d’ model architecture for both the binary classification and multi-class classification components was found to produce the most accurate patch classification for whole B. napus plant images (macro-averaged F1-score = 88.50, weighted average F1-score = 97.71). This combined binary and 5-label multi-class classification approach demonstrate similar performance to the top-performing MapReader ‘railspace’ classification model. Discussion This highlights the potential applicability of the MapReader model framework to images data from across scientific and humanities domains, and the flexibility it provides in creating pipelines with different modelling approaches. The pipeline for dynamic plant phenotyping from whole plant images developed in this study could potentially be applied to imagery from varied laboratory conditions, and to images datasets of other plants of both agricultural and conservation concern.

Why it matches plant phenotyping methods植物全体画像から葉・花・莢などの構造を自動抽出・分類する動的フェノタイピング手法を開発・評価しており、方法が研究の中心である。

abstractautomated methods capable of reliably and efficiently extracting meaningful measurements of individual plant components (e.g. leaves, flowers, pods) from imagery of whole plants are currently lacking.
Reproduction assets foundThe paper's phenotyping analysis is based on a public RGB image dataset of Brassica napus plants, explicitly deposited by the authors with a public URL. MapReader is a generic pre-existing library and the HuggingFace railspace models are cited prior work, not paper-specific assets.
Dataset · publicThe datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://research.aber.ac.uk/en/datasets/collection-of-side-view-and-top-view-rgb-images-of-brassica-napus .Open asset ↗research.aber.ac.uklines:982-1027
Code / dataset availability confirmedCrossref · Europe PMC · checked 6 Sept 2026
Published1 Mar 2025Plant PhenomicsCited by 6 · OpenAlex ↗

3D-NOD: 3D new organ detection in plant growth by a spatiotemporal point cloud deep segmentation framework

LiDAR / point cloudWhole plant / canopy / plot / fieldObject detectionOrgan identificationImage / point-cloud registrationSegmentationGrowth / time-series analysisGrowth / development / phenologyYield / yield components

Automatic plant growth monitoring is an important task in modern agriculture for maintaining high crop yield and boosting the breeding procedure. The advancement of 3D sensing technology has made 3D point clouds to be a better data form on presenting plant growth than images, as the new organs are easier identified in 3D space and the occluded organs in 2D can also be conveniently separated in 3D. Despite the attractive characteristics, analysis on 3D data can be quite challenging. We present 3D-NOD, a framework to detect new organs from time-series 3D plant data by spatiotemporal point cloud deep semantic segmentation. The design of 3D-NOD framework drew inspiration from how a well-experienced human utilizes spatiotemporal information to identify growing buds from a plant at two different growth stages. In the training phase, by introducing the Backward & Forward Labeling, the Registration & Mix-up, and the Humanoid Data Augmentation step, our backbone network can be trained to recognize growth events with organ correlation from both temporal and spatial domains. In testing, 3D-NOD has shown better sensitivity at segmenting new organs against the conventional way of using a network to conduct direct semantic segmentation. On a time-series dataset containing multiple species, Our method reached a mean F1-measure at 88.13 ​% and a mean IoU at 80.68 ​% on detecting both new and old organs with the DGCNN backbone.

Why it matches plant phenotyping methods植物の時系列3D点群から新生器官を検出・分割する手法を開発し、複数種データセットで性能評価しており、植物表現型取得が中心である。

abstractWe present 3D-NOD, a framework to detect new organs from time-series 3D plant data by spatiotemporal point cloud deep semantic segmentation.
Reproduction assets foundThe authors explicitly state that both the dataset (labeled time-series plant point clouds for tobacco, tomato, and sorghum) and the analysis code for the 3D-NOD framework are publicly available in a GitHub repository.
Dataset · publicOur data and the code are available at: https://github.com/zingersu/3D-New-Organ-Detection-in-Plant-Growth-from-Spatiotemporal-Point-Clouds.Open asset ↗https://github.com/zingersu/3D-New-Organ-Detection-in-Plant-Growth-from-Spatiotemporal-Point-Cloudshtml-lines:481-514
Code / dataset availability confirmedEurope PMC · checked 13 Sept 2026
Published10 Jul 2024Data in briefCited by 3 · OpenAlex ↗

PC4C_CAPSI: Image data of capsicum plant growth in protected horticulture.

Pepper / chilliGreenhouseLiDAR / point cloudRGB-D / ToFWhole plant / canopy / plot / fieldMorphology / geometry measurementOrgan identification2D/3D reconstructionSegmentationGrowth / development / phenology

Feeding the increasing global population and reducing the carbon footprint of agricultural activities are two critical challenges of our century. Growing crops under protected horticulture and precise crop monitoring have emerged to address these challenges. Crop monitoring in commercial protected facilities remains mostly manual and labour intensive. Using computer vision to solve specific problems in image-based crop monitoring in these compact and complex growth environments is currently hindered by the scarcity of available data. We collected an RGBD dataset for vertically supported, hydroponically-grown capsicum plants in a commercial-scale glasshouse facility to fill this gap. Data were collected weekly using a single top-angled stereo camera mounted on a mobile platform running between the hydroponic gutters. The RGBD streams covered 80 % of the crop growing season in three different light conditions. The metadata include camera configurations and light condition information. Manually measured plant heights of ten selected plants per gutter are provided as ground truth. The images covered the whole plants and focused on the top third. This dataset will support research on plant height estimation, plant organ identification, object segmentation, organ measurements, 3D reconstruction, 3D data processing, and depth noise reduction. The usability of the dataset has been successfully demonstrated in a previously published study on plant height estimation using machine learning and 3D point cloud.

Why it matches plant phenotyping methods植物の草丈推定や器官計測を目的としたRGBD画像データセットを構築し、地上真値も提供しているため、植物フェノタイピング手法・データセットが中心です。

abstractWe collected an RGBD dataset for vertically supported, hydroponically-grown capsicum plants in a commercial-scale glasshouse facility to fill this gap.
Reproduction assets foundThis data article directly deposits its paper-specific phenotyping assets: the PC4C_CAPSI RGBD image dataset (Rosbag streams, JSON metadata, manual plant-height ground truth) on the Western Sydney University ResearchDirect repository, and the authors' RGBD processing code (image extraction, depth correction, 3D reconss
Dataset · publicData accessibility Repository name: Image Data of Capsicum Plant Growth in Protected Horticulture: PC4C_CAPSI. [ 1 ] Data identification number: 10.26183/1A0R-E318 Direct URL to data: https://rds.westernsydney.edu.au/Institutes/HIE/2024/Jayasuriya_N/Open asset ↗rds.westernsydney.edu.aulines:1-40
Plant phenotyping relevance match · UnverifiedCrossref · checked 15 Sept 2026
Published29 Feb 2024Journal of Korean Institute of Intelligent SystemsCited by 0 · OpenAlex ↗

Accurate Plant Parts Recognition for Smart Farm Robot Using Deep Learning

TomatoRGB / grayscaleFruitLeafStem / branchOrgan identificationSegmentation

스마트팜에서 로봇으로 작물의 생육을 관리하기 위해 줄기, 가지, 잎, 과일 같은 작물 부위를 정밀하게 인식하는 것이 선행되어야 한다. 이 본문에서는 시맨틱 세그멘테이션 딥러닝을 이용하여 작물 부위를 인식하는 기술을 제안한다. 딥러닝 학습용 데이터셋 구축을 위해 토마토 작물의 RGB 이미지를 확보하고 라벨링하여 시맨틱 세그멘테이션 신경망 학습용 데이터셋을 구축한다. 시맨틱 세그멘테이션 신경망 U-Net을 개선하여 제안한 작물 부위 인식 기술에 적용하여 실험하고 신경망 종류에 따른 성능을 평가한다. 개선된 U-Net은 효율적 구성으로 실시간 처리가 필요한 로봇 작업에 적합하다. 제안한 작물 부위 인식 기술은 스마트팜 로봇에 접목하여 작물의 생육 관리에 활용될 것으로 기대된다.

Why it matches plant phenotyping methodsトマトの茎・枝・葉・果実を対象に、RGB画像のデータセット構築と改良U-Netによるセマンティックセグメンテーションを開発・評価しており、植物部位の画像ベース取得が研究の中心である。

titleAccurate Plant Parts Recognition for Smart Farm Robot Using Deep Learning
Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Published31 Jan 2023Frontiers in plant scienceCited by 30 · OpenAlex ↗

DFSP: A fast and automatic distance field-based stem-leaf segmentation pipeline for point cloud of maize shoot.

MaizeLiDAR / point cloudLeafStem / branchOrgan identificationSegmentation

The 3D point cloud data are used to analyze plant morphological structure. Organ segmentation of a single plant can be directly used to determine the accuracy and reliability of organ-level phenotypic estimation in a point-cloud study. However, it is difficult to achieve a high-precision, automatic, and fast plant point cloud segmentation. Besides, a few methods can easily integrate the global structural features and local morphological features of point clouds relatively at a reduced cost. In this paper, a distance field-based segmentation pipeline (DFSP) which could code the global spatial structure and local connection of a plant was developed to realize rapid organ location and segmentation. The terminal point clouds of different plant organs were first extracted via DFSP during the stem-leaf segmentation, followed by the identification of the low-end point cloud of maize stem based on the local geometric features. The regional growth was then combined to obtain a stem point cloud. Finally, the instance segmentation of the leaf point cloud was realized using DFSP. The segmentation method was tested on 420 maize and compared with the manually obtained ground truth. Notably, DFSP had an average processing time of 1.52 s for about 15,000 points of maize plant data. The mean precision, recall, and micro F1 score of the DFSP segmentation algorithm were 0.905, 0.899, and 0.902, respectively. These findings suggest that DFSP can accurately, rapidly, and automatically achieve maize stem-leaf segmentation tasks and could be effective in maize phenotype research. The source code can be found at https://github.com/syau-miao/DFSP.git.

Why it matches plant phenotyping methodsトウモロコシの点群から茎・葉を自動分割する手法を開発し、420個体の正解データで精度・速度を検証しており、植物表現型取得の中心的方法である。

abstracta distance field-based segmentation pipeline (DFSP) which could code the global spatial structure and local connection of a plant was developed to realize rapid organ location and segmentation.
Plant phenotyping relevance match · UnverifiedEurope PMC · bioRxiv · checked 8 Sept 2026
Published26 Nov 2022bioRxivCited by 0 · OpenAlex ↗

Self-Supervised Maize Kernel Classification and Segmentation for Embryo Identification

MaizeSeed / grainAnnotation / quality controlClassificationOrgan identificationSegmentation

ABSTRACT Computer vision and deep learning (DL) techniques have succeeded in a wide range of diverse fields. Recently, these techniques have been successfully deployed in plant science applications to address food security, productivity, and environmental sustainability problems for a growing global population. However, training these DL models often necessitates the large-scale manual annotation of data which frequently becomes a tedious and time-and-resource-intensive process. Recent advances in self-supervised learning (SSL) methods have proven instrumental in overcoming these obstacles, using purely unlabeled datasets to pre-train DL models. Here, we implement the popular self-supervised contrastive learning methods of NNCLR (Nearest neighbor Contrastive Learning of visual Representations) and SimCLR (Simple framework for Contrastive Learning of visual Representations) for the classification of spatial orientation and segmentation of embryos of maize kernels. Maize kernels are imaged using a commercial high-throughput imaging system. This image data is often used in multiple downstream applications across both production and breeding applications, for instance, sorting for oil content based on segmenting and quantifying the scutellum’s size and for classifying haploid and diploid kernels. We show that in both classification and segmentation problems, SSL techniques outperform their purely supervised transfer learning-based counterparts and are significantly more annotation efficient. Additionally, we show that a single SSL pre-trained model can be efficiently finetuned for both classification and segmentation, indicating good transferability across multiple downstream applications. Segmentation models with SSL-pretrained backbones produce DICE similarity coefficients of 0.81, higher than the 0.78 and 0.73 of those with ImageNet-pretrained and randomly initialized backbones, respectively. We observe that finetuning classification and segmentation models on as little as 1% annotation produces competitive results. These results show SSL provides a meaningful step forward in data efficiency with agricultural deep learning and computer vision.

Why it matches plant phenotyping methodsトウモロコシ種子胚の分類・セグメンテーションに対する自己教師あり画像解析手法を開発・比較検証しており、植物形質の取得・抽出が研究の中心です。

titleSelf-Supervised Maize Kernel Classification and Segmentation for Embryo Identification
Code / dataset availability confirmedOpenAlex · Europe PMC · Crossref · checked 8 Sept 2026
Published10 Nov 2022Frontiers in Plant ScienceCited by 31 · OpenAlex ↗

A graph-based approach for simultaneous semantic and instance segmentation of plant 3D point clouds

SpinachTomatoLiDAR / point cloudLeafStem / branchWhole plant / canopy / plot / fieldMorphology / geometry measurementOrgan identificationSegmentation

Accurate simultaneous semantic and instance segmentation of a plant 3D point cloud is critical for automatic plant phenotyping. Classically, each organ of the plant is detected based on the local geometry of the point cloud, but the consistency of the global structure of the plant is rarely assessed. We propose a two-level, graph-based approach for the automatic, fast and accurate segmentation of a plant into each of its organs with structural guarantees. We compute local geometric and spectral features on a neighbourhood graph of the points to distinguish between linear organs (main stem, branches, petioles) and two-dimensional ones (leaf blades) and even 3-dimensional ones (apices). Then a quotient graph connecting each detected macroscopic organ to its neighbors is used both to refine the labelling of the organs and to check the overall consistency of the segmentation. A refinement loop allows to correct segmentation defects. The method is assessed on both synthetic and real 3D point-cloud data sets of Chenopodium album (wild spinach) and Solanum lycopersicum (tomato plant).

Why it matches plant phenotyping methods植物3D点群から器官を自動分割・識別するグラフベース手法を開発し、合成および実データで評価しており、植物表現型取得の技術が中心である。

abstractAccurate simultaneous semantic and instance segmentation of a plant 3D point cloud is critical for automatic plant phenotyping.
Reproduction assets foundThe paper's Chenopodium 3D point cloud dataset (with ground truth annotations) is publicly deposited on Zenodo, and the reconstruction pipeline code is open source on GitHub (romi/plant-3d-vision).
Dataset · publicThe datasets presented in this study can be found in online repositories. The names of the repository/repositories and accession number(s) can be found below: https://zenodo.org/record/6962994#.YuvYkS8itqs .Open asset ↗zenodo · 6962994lines:641-715
Code · publicThe entire code is open source and available online ( https://github.com/romi/plant-3d-vision ).Open asset ↗github · romi/plant-3d-visionlines:428-438
Code / dataset availability confirmedCrossref · checked 13 Sept 2026
Published13 Sept 2022AgricultureCited by 26 · OpenAlex ↗

Segmentation and Stratification Methods of Field Maize Terrestrial LiDAR Point Cloud

MaizeField / plotLiDAR / point cloudPanicle / ear / spikeLeafWhole plant / canopy / plot / fieldMorphology / geometry measurementOrgan identificationSegmentationLeaf traits

Three-dimensional (3D) laser point cloud technology is an important research method in the field of agricultural remote sensing research. The collection and processing technology of terrestrial light detection and ranging (LiDAR) point cloud of crops has greatly promoted the integration of agricultural informatization and intelligence. In a smart farmland based on 3D modern agriculture, the manager can efficiently and conveniently achieve the growth status of crops through the point cloud collection system and processing model integrated in the smart agricultural system. To this end, we took field maize as the research object in this study and processed four sets of field maize point clouds, named Maize-01, Maize-02, Maize-03, and Maize-04, respectively. In this research, we established a field individual maize segmentation model with the density-based clustering algorithm (DBSCAN) as the core, and four groups of field maize were used as research objects. Among them, the value of the overall accuracy (OA) index, which was used to evaluate the comprehensive performance of the model, were 0.98, 0.97, 0.95, and 0.94. Secondly, the multi-condition identification method was used to separate different maize organ point clouds from the individual maize point cloud. In addition, the organ stratification model of field maize was established. In this organ stratification study, we take Maize-04 as the research object and obtained the recognition accuracy rates of four maize organs: tassel, stalk, ear, and leaf at 96.55%, 100%, 100%, and 99.12%, respectively. We also finely segmented the leaf organ obtained from the above-mentioned maize organ stratification model into each leaf individual again. We verified the accuracy of the leaf segmentation method with the leaf length as the representative. In the linear analysis of predicted values of leaf length, R2 was 0.73, RMSE was 0.12 m, and MAE was 0.07 m. In this study, we examined the segmentation of individual crop fields and established 3D information interpretations for crops in the field as well as for crop organs. Results visualized the real scene of the field, which is conducive to analyzing the response mechanism of crop growth and development to various complex environmental factors.

Why it matches plant phenotyping methods圃場トウモロコシのLiDAR点群から個体・器官・葉を分割し、葉長を推定・検証する手法が研究の中心であり、再利用可能な植物表現型抽出ワークフローに該当する。

abstractwe established a field individual maize segmentation model with the density-based clustering algorithm (DBSCAN) as the core
Reproduction assets foundThe paper's Data Availability Statement explicitly deposits the authors' PCL/C++ segmentation and stratification code at a public GitHub repository matching an allowed URL. The maize point cloud dataset is also stated to be online at scidb.cn, but the full dataset URL does not exactly match any allowed_urls entry, so a
Code · publicThe main PCL/C++ code in this paper are available online at https://github.com/1117ismore/HZAU-Segmentation-Stratification-pcd.gitOpen asset ↗HZAU-Segmentation-Stratification-pcdpdf-page:18 lines:1-56
Plant phenotyping relevance match · UnverifiedOpenAlex · checked 13 Sept 2026
Published30 Aug 2022Remote SensingCited by 8 · OpenAlex ↗

An Unsupervised Canopy-to-Root Pathing (UCRP) Tree Segmentation Algorithm for Automatic Forest Mapping

Aerial / UAVField / plotPhotogrammetry / SfM / MVSLiDAR / point cloudStem / branchWhole plant / canopy / plot / fieldOrgan identificationSegmentation

Terrestrial laser scanners, unmanned aerial LiDAR, and unmanned aerial photogrammetry are increasingly becoming the go-to methods for forest analysis and mapping. The three-dimensionality of the point clouds generated by these technologies is ideal for capturing the structural features of trees such as trunk diameter, canopy volume, and biomass. A prerequisite for extracting these features from point clouds is tree segmentation. This paper introduces an unsupervised method for segmenting individual trees from point clouds. Our novel, canopy-to-root, least-cost routing method segments trees in a single routine, accomplishing stem location and tree segmentation simultaneously without needing prior knowledge of tree stem locations. Testing on benchmark terrestrial-laser-scanned datasets shows that we achieve state-of-the-art performances in individual tree segmentation and stem-mapping accuracy on boreal and temperate hardwood forests regardless of forest complexity. To support mapping at scale, we test on unmanned aerial photogrammetric and LiDAR point clouds and achieve similar results. The proposed algorithm’s independence from a specific data modality, along with its robust performance in simple and complex forest environments and accurate segmentation results, make it a promising step towards achieving reliable stem-mapping capabilities and, ultimately, towards building automatic forest inventory procedures.

Why it matches plant phenotyping methods森林点云から個体樹木を分割し、幹位置や樹木構造形質の抽出を可能にするアルゴリズム開発・ベンチマークであり、植物形質取得法が中心です。

abstractThis paper introduces an unsupervised method for segmenting individual trees from point clouds.
Code / dataset availability confirmedOpenAlex · Crossref · Europe PMC · checked 8 Sept 2026
Published18 Apr 2022Frontiers in Plant ScienceCited by 2 · OpenAlex ↗

High-Throughput 3D Phenotyping of Plant Shoot Apical Meristems From Tissue-Resolution Data

ArabidopsisAerial / UAVMicroscopyFlowerTissueMorphology / geometry measurementOrgan identification2D/3D reconstructionSegmentationArchitecture / morphology / geometry

Confocal imaging is a well-established method for investigating plant phenotypes on the tissue and organ level. However, many differences are difficult to assess by visual inspection and researchers rely extensively on ad hoc manual quantification techniques and qualitative assessment. Here we present a method for quantitatively phenotyping large samples of plant tissue morphologies using triangulated isosurfaces. We successfully demonstrate the applicability of the approach using confocal imaging of aerial organs in Arabidopsis thaliana. Automatic identification of flower primordia using the surface curvature as an indication of outgrowth allows for high-throughput quantification of divergence angles and further analysis of individual flowers. We demonstrate the throughput of our method by quantifying geometric features of 1065 flower primordia from 172 plants, comparing auxin transport mutants to wild type. Additionally, we find that a paraboloid provides a simple geometric parameterisation of the shoot inflorescence domain with few parameters. We utilise parameterisation methods to provide a computational comparison of the shoot apex defined by a fluorescent reporter of the central zone marker gene CLAVATA3 with the apex defined by the paraboloid. Finally, we analyse the impact of mutations which alter mechanical properties on inflorescence dome curvature and compare the results with auxin transport mutants. Our results suggest that region-specific expression domains of genes regulating cell wall biosynthesis and local auxin transport can be important in maintaining the wildtype tissue shape. Altogether, our results indicate a general approach to parameterise and quantify plant development in 3D, which is applicable also in cases where data resolution is limited, and cell segmentation not possible. This enables researchers to address fundamental questions of plant development by quantitative phenotyping with high throughput, consistency and reproducibility.

Why it matches plant phenotyping methods植物組織の3D画像から形態形質を自動抽出・定量する手法を開発し、高スループット性と再現性を実証しているため、フェノタイピング手法が中心である。

abstractHere we present a method for quantitatively phenotyping large samples of plant tissue morphologies using triangulated isosurfaces.
Reproduction assets foundThe paper's data availability statement explicitly deposits all original source data (confocal phenotyping data of Arabidopsis shoot apical meristems) in the Cambridge Apollo repository and all analysis/segmentation/quantification scripts in a public Sainsbury Laboratory GitLab repository. Both are paper-specific,公开,直接
Dataset · publicAll original source data files used in this study are available via the Cambridge University Apollo Repository ( https://doi.org/10.17863/CAM.82442 ).Open asset ↗Cambridge University Apollo Repository · 10.17863/CAM.82442lines:369-397
Code · publicAll scripts and software for segmentation, quantification, analysis and visualisation are available via the Sainsbury Laboratory GitLab repository ( https://gitlab.com/slcu/teamHJ/publications/aahl_etal_2022 ).Open asset ↗Sainsbury Laboratory GitLab repositorylines:369-397
Plant phenotyping relevance match · UnverifiedEurope PMC · checked 15 Sept 2026
Published1 Dec 2020Biosystems engineering.Cited by 25 · OpenAlex ↗

Automated classification of stems and leaves of potted plants based on point cloud data

LiDAR / point cloudLeafStem / branchClassificationOrgan identification

The accurate classification of plant organs is a key step in monitoring the growing status and physiology of plants. A classification method was proposed to classify the leaves and stems automatically based on the point cloud data of the potted plants. Leaf samples and stem samples were selected automatically by using the three-dimensional (3D) convex hull algorithm and the two-dimensional (2D) projection grid density respectively, and were used to construct the leaf and stem training sets. Then, the point cloud data were classified into leaf points and stem points by using the support vector machine (SVM) algorithm. The point cloud data of three potted plants were used in the experiment. The proposed method was compared with the standard classification, the random selection method and the manual selection method. Among these methods, the proposed method is automated and time-saving. The results show that the proposed method had a good overall performance on accuracy and running time. The proposed method is efficient and effective on the leaf and stem classification of the plant point cloud data.

Why it matches plant phenotyping methods植物の点群データから葉と茎を自動分類する手法の開発と比較検証が研究の中心であり、器官構造の表現型抽出に該当する。

abstractA classification method was proposed to classify the leaves and stems automatically based on the point cloud data of the potted plants.
Plant phenotyping relevance match · UnverifiedOpenAlex · bioRxiv · Europe PMC · Crossref · checked 11 Sept 2026
Published12 May 2016bioRxivCited by 44 · OpenAlex ↗

Deep Machine Learning provides state-of-the-art performance in image-based plant phenotyping

Field / plotRootWhole plant / canopy / plot / fieldObject detectionOrgan identification

Abstract Deep learning is an emerging field that promises unparalleled results on many data analysis problems. We show the success offered by such techniques when applied to the challenging problem of image-based plant phenotyping, and demonstrate state-of-the-art results for root and shoot feature identification and localisation. We predict a paradigm shift in image-based phenotyping thanks to deep learning approaches.

Why it matches plant phenotyping methods画像ベース植物フェノタイピングに深層学習を適用し、根とシュートの特徴の同定・局在化性能を示す手法研究であり、フェノタイプ抽出が中心である。

abstractWe show the success offered by such techniques when applied to the challenging problem of image-based plant phenotyping, and demonstrate state-of-the-art results for root and shoot feature identification and localisation.