Integrating imaging sensors and artificial intelligence (AI) have contributed to detecting plant stress symptoms, yet data analysis remains a key challenge. Data challenges include standardized data collection, analysis protocols, selection of imaging sensors and AI algorithms, and finally, data sharing. Here, we present a systematic literature review (SLR) scrutinizing plant imaging and AI for identifying stress responses. We performed a scoping review using specific keywords, namely abiotic and biotic stress, machine learning, plant imaging and deep learning. Next, we used programmable bots to retrieve relevant papers published since 2006. In total, 2,704 papers from 4 databases (Springer, ScienceDirect, PubMed, and Web of Science) were found, accomplished by using a second layer of keywords (e.g., hyperspectral imaging and supervised learning). To bypass the limitations of search engines, we selected OneSearch to unify keywords. We carefully reviewed 262 studies, summarizing key trends in AI algorithms and imaging sensors. We demonstrated that the increased availability of open-source imaging repositories such as PlantVillage or Kaggle has strongly contributed to a widespread shift to deep learning, requiring large datasets to train in stress symptom interpretation. Our review presents current trends in AI-applied algorithms to develop effective methods for plant stress detection using image-based phenotyping. For example, regression algorithms have seen substantial use since 2021. Ultimately, we offer an overview of the course ahead for AI and imaging technologies to predict stress responses. Altogether, this SLR highlights the potential of AI imaging in both biotic and abiotic stress detection to overcome challenges in plant data analysis.
Why it matches plant phenotyping methods植物ストレス検出のための画像センサーとAI手法を体系的にレビューしており、植物表現型取得・解析手法が中心である。
abstractOur review presents current trends in AI-applied algorithms to develop effective methods for plant stress detection using image-based phenotyping.
Reproduction assets foundThe paper's authors publicly released the programmable-bot Python code used to conduct the systematic literature review's database searches and data processing, with explicit availability language and a GitHub URL. The Zotero group library of 262 studies is public but has no URL in the allowed list; Kaggle/Zindi/SpectrCode · publicJ.J.W. and E.M.; data curation and visualisation: J.J.W. writing original draft: all authors; writing, review and editing: J.J.W. and S.N.; funding acquisition: S.N.
Competing interests: The authors declare no conflict of interest.
Data Availability
All code used to create and run the programmable bots is available on GitHub ( https://github.com/Walshj73/data-processing-bot.git ) and licensed under the MIT license. All 262 studies found during this SLR process are available in a publicly accessible Zotero group library (titled “Advancements in Imaging Sensors and AI for Plant Stress Detection”). The group library can be accessed on Zotero by using the “Search for groups” feature found under Open asset ↗Walshj73/data-processing-botlines:160-199Code / dataset availability confirmedCrossref · Europe PMC · checked 13 Sept 2026
Abstract Plant pathogens can decimate crops and render the local cultivation of a species unprofitable. In extreme cases this has caused famine and economic collapse. Timing is vital in treating crop diseases, and the use of computer vision for precise disease detection and timing of pesticide application is gaining popularity. Computer vision can reduce labour costs, prevent misdiagnosis of disease, and prevent misapplication of pesticides. Pesticide misapplication is both financially costly and can exacerbate pesticide resistance and pollution. Here, we review the application and development of computer vision and machine learning methods for the detection of plant disease. This review goes beyond the scope of previous works to discuss important technical concepts and considerations when applying computer vision to plant pathology. We present new case studies on adapting standard computer vision methods and review techniques for acquiring training data, the use of diagnostic tools from biology, and the inspection of informative features. In addition to an in‐depth discussion of convolutional neural networks (CNNs) and transformers, we also highlight the strengths of methods such as support vector machines and evolved neural networks. We discuss the benefits of carefully curating training data and consider situations where less computationally expensive techniques are advantageous. This includes a comparison of popular model architectures and a guide to their implementation.
Why it matches plant phenotyping methods植物病害を対象としたコンピュータビジョンによる症状・病害の検出手法を中心に扱うレビューであり、植物フェノタイピング手法の方法論的整理と評価が主題である。
abstractHere, we review the application and development of computer vision and machine learning methods for the detection of plant disease.
Reproduction assets foundThe paper's data availability statement provides public OSF deposits (view-only links) containing image data, annotations, training data, and semi-supervised model weights for the cocoa disease-detection case studies, plus public GitHub repositories with the authors' custom training/analysis code (CocoaReader, CocoaNetDataset · publicThe image data, annotations, and link to the accompanying GitHub repository for Case Study 1 can be found at: https://osf.io/79kx3/?view_only=4a2c1dccee1a4baeb85de5002c702f10 .Open asset ↗osflines:411-466Dataset · publicFor Case Study 2, the data used to train the initial supervised model, the .csv search terms file for the below web scraper, and the final semi‐supervised model weights can be found at: https://osf.io/h5gj7/?view_only=dbf9f245e21a41e185f5b73e718b4cad .Open asset ↗osflines:411-466Code · publicThe custom code used to train both the initial model and the final semi‐supervised model can be found at: https://github.com/jrsykes/CocoaReader/blob/main/PlantNotPlant .Open asset ↗github · jrsykes/CocoaReaderlines:411-466Code · publicThe custom code to run the sweep in Case Study 4 can be found in the following GitHub repository: https://github.com/jrsykes/CocoaReader/tree/main/CocoaNet .Open asset ↗github · jrsykes/CocoaReaderlines:411-466Dataset · publicThe data used to generate these results and the full wandb report can be found at: https://osf.io/2fw6g/?view_only=adc66ba66f83465a9e7b111515a60bf2 .Open asset ↗osflines:411-466Code · publicThe “contaminated” data used to train the semi‐supervised model were generated using the code at: https://github.com/jrsykes/Google-Image-Scraper .Open asset ↗github · jrsykes/Google-Image-Scraperlines:411-466Code / dataset availability confirmedEurope PMC · bioRxiv · checked 8 Sept 2026
Field / plotRootWhole plant / canopy / plot / fieldRoot system architecture
Root phenotyping describes methods for measuring root properties, or traits. While root phenotyping can be challenging, it is advancing quickly. In order for the field to move forward, it is essential to understand the current state and challenges of root phenotyping, as well as the pressing needs of the root biology community. In this letter, we present and discuss the results of a survey that was created and disseminated by members of the Graduate Student and Postdoc Ambassador Program at the 11th symposium of the International Society of Root Research. This survey aimed to (1) provide an overview of the objectives, biological models and methodological approaches used in root phenotyping studies, and (2) identify the main limitations currently faced by plant scientists with regard to root phenotyping. Our survey highlighted that (1) monocotyledonous crops dominate the root phenotyping landscape, (2) root phenotyping is mainly used to quantify morphological and architectural root traits, (3) 2D root scanning/imaging is the most widely used root phenotyping technique, (4) time-consuming tasks are an important barrier to root phenotyping, (5) there is a need for standardised, high-throughput methods to sample and phenotype roots, particularly under field conditions, and to improve our understanding of trait-function relationships.
Why it matches plant phenotyping methods根系フェノタイピングの手法、利用状況、限界、標準化ニーズを調査・整理したレビュー的研究であり、フェノタイピング方法論が中心です。
abstractRoot phenotyping describes methods for measuring root properties, or traits.
Reproduction assets foundThe paper's Data availability statement explicitly deposits the survey raw data and R analysis code on Zenodo (DOI 10.5281/zenodo.5901959), a public, paper-specific, actionable asset. The Nottingham Hidden Half maize image URL is only a credited Figure 1 image source, not a paper-specific dataset, and is not listed asaCode · publicRaw data and R code are available on Zenodo at https://doi.org/10.5281/zenodo.5901959.Open asset ↗Zenodo · 10.5281/zenodo.5901959pdf-page:10 lines:1-39