Root restriction accelerates genomic target identification in quinoa under controlled conditions.

Abstract

Quinoa (Chenopodium quinoa) is a nutritious and resilient crop that displays a high genetic and phenotypic variation. As the popularity of this crop increases, there is a growing need to integrate classic and modern breeding tools to favor its improvement. We tested root restriction as a method to reduce plant size and enable high-throughput phenotypic screening of large sets of quinoa plants under controlled conditions. We verified how increasing root restriction does not affect the prediction of field behavior with respect to other standard greenhouse cultivation procedures. We then combined the phenotypic information obtained with our root restriction system with whole-genome re-sequencing data to characterize a quinoa diversity panel of 100 accessions and showed that phenotypic data obtained from root-restricted plants provide real insights into quinoa genetics. Finally, we carried out a genome-wide association study (GWAS) and identified a previously described locus for betalain biosynthesis, as well as other candidate loci linked to betalain biosynthesis and seed size. Overall, we showed that a phenotyping system based on root restriction can aid the identification of genomic targets in quinoa, which can complement and inform field trials for certain traits. This work supports further breeding and faster improvement of quinoa.

Plant phenotyping relevance

根域制限を利用したハイスループット表現型スクリーニング系の構築・検証と、圃場挙動予測との比較が研究の中心であり、単なる生物学的測定ではない。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

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