Measurement of Cyclic GMP During Plant Hypersensitive Disease Resistance Response.

Abstract

Cyclic guanosine-3',5'-monophosphate (cGMP) is recognized as an important second messenger in plants, mediating intracellular signal in important physiological processes, including the hypersensitive disease resistance response induced by avirulent pathogens. In this context, the analysis of cGMP levels in infected plants requires an accurate and specific detection method allowing its quantification. Here, we describe an assay based on the Alphascreen technology, developed for animal cells and further adapted and optimized for the detection of cGMP in plants. The method is applied for the measurement of cGMP in Arabidopsis thaliana plants challenged with an avirulent strain of Pseudomonas syringae pv. tomato. This protocol includes the extraction of cGMP, the assay procedure and the calculation of cGMP concentration.

Plant phenotyping relevance

植物中のcGMPを定量する検出アッセイを動物細胞用技術から適応・最適化し、感染植物への適用手順まで示した方法論論文であり、植物の生理状態測定法が中心です。

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