Non-Destructive Inspection of Physicochemical Indicators of Lettuce at Rosette Stage Based on Visible/Near-Infrared Spectroscopy.

Abstract

Lettuce is a globally important cash crop, valued by consumers for its nutritional content and pleasant taste. However, there is limited research on the changes in the growth indicators of lettuce during its growth period in domestic settings. Quality assessment primarily relies on subjective evaluations, resulting in significant variability. This study focused on hydroponically grown lettuce during the rosette stage and investigated the patterns of changes in the indicators and spectral curves over time. By employing spectral preprocessing and selecting characteristic wavelengths, three models were developed to predict the indicators. The results showed that the optimal model structures were S_G-UVE-PLSR (SSC and vitamin C) and Nor-CARS-PLSR (moisture content). The PLSR models achieved prediction set correlation coefficients of 0.8648, 0.8578, and 0.8047, with residual prediction deviations of 1.9685, 1.9568, and 1.6689, respectively. The optimal models were integrated into a portable device, using real-time analysis software written in Matlab2021a, for the prediction of the physicochemical indicators of lettuce during the rosette stage. The results demonstrated prediction set correlation coefficients of 0.8215, 0.8472, and 0.7671, with root mean square errors of prediction of 0.5348, 1.5813, and 2.3347 for a sample size of 180. The small discrepancies between the predicted and actual values indicate that the developed device can meet the requirements for real-time detection.

Plant phenotyping relevance

可視・近赤外分光と波長選択、PLSRモデルおよび携帯型リアルタイム装置を開発し、レタスの水分・ビタミンC・可溶性固形分を非破壊推定する方法が研究の中心である。

Code and data availability

The paper reports lettuce Vis/NIR spectral and physicochemical measurements (SSC, moisture, vitamin C; 180 samples) and PLSR modeling, but no public dataset, code, or model deposit is provided. The Data Availability Statement only directs inquiries to the corresponding authors, so any data must be requested from them.

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