Application of fluorescence i-motif DNA silver nanocluster sensor to visualize endogenous reactive oxygen species in plant cells

Abstract

Abstract Reactive oxygen species (ROS) are crucial in plant growth, defense, and stress responses, making them vital for improving crop resilience. Various ROS sensing methods for plants have been developed to detect ROS in vitro and in vivo . However, each method comes its own advantages and disadvantages, leading to an increasing demand for a simple and effective sensory system for ROS detection in plants. Here, we introduce novel DNA silver nanoclusters (DNA/AgNCs) sensors for visualizing ROS in plants. Two sensors, C 20 /AgNCs and FAM-C 20 /AgNCs-Cy5, detect intracellular ROS signaling in response to stimuli such as abscisic acid, salicylic acid, ethylene, and bacterial peptide elicitor flg22. Notably, FAM-C 20 /AgNCs-Cy5 exceeds the sensing capabilities of HyPer7, a widely recognized ROS sensor. Taken together, we suggest that fluorescent i-motif DNA/AgNCs system is an effective tool for visualizing ROS signals in plant cells. This advancement is important to advancing our understanding of ROS-mediated processes in plant biology.

Plant phenotyping relevance

植物細胞内ROSという生理状態を可視化する新規DNA/AgNCsセンサーを開発・比較評価しており、植物表現型の取得法が研究の中心である。

Code and data availability

The article describes DNA/AgNC ROS nanosensor experiments in plants but contains no public phenotype dataset, image/sensor data deposit, author analysis code, or trained model. The only supplement mentioned is 'SupplementaryFIgures.pptx' (figures only, no data/code), and all listed URLs are cited references, not paper-

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