Unverified paper record
A robust estimation method for canopy chlorophyll content based on FOD and hierarchical weighting combination models
European Journal of Agronomy. · 1 Mar 2026
Abstract
Assessing canopy chlorophyll content (CCC) is crucial for evaluating light capture and photosynthetic capacity, as well as for diagnosing and managing maize health. This study aims to develop a robust CCC estimation model using in situ canopy spectral data collected from two regions over a three-year period. The model employs fractional order differential (FOD) and partial least squares regression (PLSR) at multiple spectral resolutions (1 nm, 5 nm, 10 nm, 20 nm, and Sentinel-2 broadband). To mitigate the uncertainties associated with single models and enhance estimation accuracy, a hierarchical weighted combination model integrating k-means clustering and genetic algorithm (GA) is proposed. The results indicate that the CCC estimation models constructed from differential spectra generally outperform those based on original spectra across most orders. The optimal estimation orders are typically within the range of 1.2–1.6 (step: 0.2). For each resolution, the root mean square error (RMSE) of the optimal order in the test set is reduced by 1.65–25.04 % compared to the original spectra. After constructing the hierarchical weighted combination prediction model, the R² and RMSE of the combined model for each resolution are superior to those of the single models. Specifically, the RMSE of the test set is further reduced by 0.38–9.87 % compared to the optimal FOD order. Moreover, we achieved better monitoring results when we migrated the method to remotely sensed images. These findings suggest that the hierarchical weighted combination prediction model driven by fractional order differential spectra can achieve more accurate CCC estimation in maize. This method provides a basis for applying FOD to multi-resolution sensors, and this achievement contributes to the precise regulation of fertilizer and water during maize growth, offering a new technical approach for improving crop yield and resource use efficiency.
Plant phenotyping relevance
トウモロコシ群落クロロフィル量という植物形質を対象に、分光データ、FOD、PLSR、階層重み付けモデルによる推定手法を開発・検証しており、表現型取得・抽出が研究の中心である。
abstractThis study aims to develop a robust CCC estimation model using in situ canopy spectral data collected from two regions over a three-year period.
abstracta hierarchical weighted combination model integrating k-means clustering and genetic algorithm (GA) is proposed.
abstractThe results indicate that the CCC estimation models constructed from differential spectra generally outperform those based on original spectra across most orders.
abstractThis method provides a basis for applying FOD to multi-resolution sensors
Code and data availability
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