cknowledgments The authors whish to thank A. Costa, M. Grenzi and NOLIMITS, an advanced imaging facility established by the University of Milan, for providing the video and images of the fast calcium imaging analyses upon S. littoralis herbivory. Supplementary Materials The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/plants11202689/s1 , Video S1: Rapid Ca 2+ signals generated when Spodoptera littoralis feeds on Arabidopsis thaliana expressing the genetically encoded R-GECO1 sensor. Click here for additional data file. Author Contributions Conceptualization, M.E.M.; methodology, M.E.M.; investigation, A.S.P. and M.E.M.; resources, M.E.M.; writin
Open resource ↗lines:66-85Unverified paper record
Calcium Signaling in Plant-Insect Interactions
Plants · 12 Oct 2022 · 10.3390/plants11202689
Abstract
In plant–insect interactions, calcium (Ca2+) variations are among the earliest events associated with the plant perception of biotic stress. Upon herbivory, Ca2+ waves travel long distances to transmit and convert the local signal to a systemic defense program. Reactive oxygen species (ROS), Ca2+ and electrical signaling are interlinked to form a network supporting rapid signal transmission, whereas the Ca2+ message is decoded and relayed by Ca2+-binding proteins (including calmodulin, Ca2+-dependent protein kinases, annexins and calcineurin B-like proteins). Monitoring the generation of Ca2+ signals at the whole plant or cell level and their long-distance propagation during biotic interactions requires innovative imaging techniques based on sensitive sensors and using genetically encoded indicators. This review summarizes the recent advances in Ca2+ signaling upon herbivory and reviews the most recent Ca2+ imaging techniques and methods.
Plant phenotyping relevance
植物のCa2+シグナルを測定するイメージング技術・手法をレビューしており、植物の生理状態の取得方法が中心的に扱われている。
abstractMonitoring the generation of Ca2+ signals at the whole plant or cell level and their long-distance propagation during biotic interactions requires innovative imaging techniques based on sensitive sensors and using genetically encoded indicators.
abstractThis review summarizes the recent advances in Ca2+ signaling upon herbivory and reviews the most recent Ca2+ imaging techniques and methods.
Code and data availability
The paper's own supplementary material contains Video S1, the authors' calcium imaging time-course of Spodoptera littoralis feeding on R-GECO1-expressing Arabidopsis, publicly downloadable from the MDPI supplementary URL.
This is an automatically classified, unverified record. Curator approval is required before any resource enters the Catalog.