Hyperspectral Imagery Detects Water Deficit and Salinity Effects on Photosynthesis and Antioxidant Enzyme Activity of Three Greek Olive Varieties

Abstract

The olive tree (Olea europaea L.) is one of the main crops of the Mediterranean region which suffers from drought and soil salinization. We assessed the photosynthetic rate, leaf water content and antioxidative enzyme activity (APX, GPX, SOD and CAT) of three Greek olive cultivars (‘Amfisis’, ‘Mastoidis’ and ‘Lefkolia Serron’) subjected to drought and salinity stresses. Hyperspectral reflectance data were acquired using an analytical spectral device (ASD) FieldSpec® 3 spectroradiometer, while principal component regression, partial least squares regression and linear discriminant analysis were used to estimate the relationship between spectral and physiological measurements. The photosynthetic rate and water content of stressed plants decreased, while enzyme activity had an increasing tendency. ‘Amfisis’ was more resistant to drought and salinity stress than ‘Mastoidis’ and ‘Lefkolia Serron’. The NDVI appeared to have the highest correlation with the photosynthetic rate, followed by the PRI. APX enzyme activity was the most highly correlated with the 1150–1370 nm range, with an additional peak at 1840 nm. CAT enzyme activity resulted in the highest correlation with the visible part of the spectrum with two peaks at 1480 nm and 1950 nm, while GPX enzyme activity appeared to have a strong correlation within all the available spectral ranges except for 670–1180 nm. Finally, SOD activity showed high correlation values within 1190–1850 nm. This is the first time the correlation of hyperspectral imagery with photosynthetic rate and antioxidant enzyme activities was determined, providing the background for high-throughput plant phenotyping through a drone with a hyperspectral camera. This progress would provide the possibility of early stress detection in large olive groves and assist farmers in decision making and optimizing crop management, health and productivity.

Plant phenotyping relevance

ハイパースペクトル計測と回帰・判別モデルにより、光合成速度や抗酸化酵素活性などの植物生理状態を推定し、高スループット表現型解析への応用可能性を評価しているため、手法が中心的です。

Code and data availability

The paper reports hyperspectral, photosynthesis, and antioxidant enzyme measurements of three Greek olive cultivars, but no public dataset, code, or model deposit is identified. The Data Availability Statement only offers data on request: 'Data are available by the authors upon reasonable request.' All URLs in the text

No evidence-backed public reproduction asset is currently recorded.