A live-imaging system for Arabidopsis leaf primordia at early stages

Abstract

Live imaging is one of the most powerful methods to reveal the morphogenesis of plant organs. However, the highly three-dimensional structure of plant organs always poses technical challenges. For example, the basal region of leaf primordia is rarely observed because of the shape of leaf primordia and the sudden shift in geometry at the point where the leaf primordium connects to the hypocotyl. In this work, we developed a new live-imaging system that is suitable for observing the developmental process of the basal region of Arabidopsis leaf primordia at early stages. Using this system, we achieved continuous observation of the basal region of early Arabidopsis leaf primordia for more than 50 hours.

Plant phenotyping relevance

シロイヌナズナ葉原基の発生過程を継続観察する新規ライブイメージングシステムの開発が主題であり、植物器官の形態・発生状態の取得方法が中心的に扱われている。

Code and data availability

The preprint describes a live-imaging system for Arabidopsis leaf primordia but contains no public phenotype/trait dataset, image deposit, analysis code, or model availability statement. The only accession mentioned (ABRC CS73343) is a seed line from prior work, not a paper-specific public asset. No data or code URLs,

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