A Stomata Classification and Detection System in Microscope Images of Maize Cultivars

Abstract

Research on stomata, i.e., morphological structures of plants, has increased in popularity in the last years. These structures (pores) are in charge of the interaction between the internal plant system and the environment, working on different processes such as photosynthesis and transpiration stream. Besides, a better understanding of the pore mechanism plays a significant role when exploring the evolution process, as well as the behavior of plants. Although the study of stomata in dicots species of plants has advanced considerably in the past years, there is little information about stomata of cereal grasses. Also, automated detection of these structures have been considered in the literature, but some gaps are still uncovered. This fact is motivated by high morphological variation of stomata and the presence of noise from the image acquisition step. In this work, we propose a new methodology for automatic stomata classification and a new detection system in microscope images for maize cultivars. We have achieved an approximated accuracy of 97.1% in the identification of stomata regions using classifiers based on deep learning features, which figures out as a nearly perfect classification system.

Plant phenotyping relevance

トウモロコシの気孔を顕微鏡画像から自動分類・検出する画像ベースの植物表現型取得法を開発しており、方法が研究の中心である。

Code and data availability

The paper describes a maize stomata classification/detection system with a 200-image microscope dataset and 2,000 labeled subimages, but no supplied block contains any data or code availability statement, public repository link, or author URL for the dataset, images, models, or analysis code. Only generic library URLs,

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