Quantification of Starch in Guard Cells of Arabidopsis thaliana .

Abstract

In this protocol, we describe how to quantify starch in guard cells of Arabidopsis thaliana using the fluorophore propidium iodide and confocal laser scanning microscopy. This simple method enables monitoring, with unprecedented resolution, the dynamics of starch in guard cells.

Plant phenotyping relevance

ガード細胞内デンプンを蛍光標識と共焦点顕微鏡で定量する具体的な画像計測プロトコルが研究の中心であり、植物の生理状態を測定する方法に該当する。

Code and data availability

This is a methods/protocol article describing guard cell starch quantification via propidium iodide staining and confocal imaging. The only software mentioned is ImageJ, a generic public analysis tool, not an authors' paper-specific asset; no phenotype datasets, images, code repositories, models, or supplements with a

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