3D Visualization of Microtubules in Epidermal Pavement Cells.

Abstract

The plant cytoskeleton is instrumental in cellular processes such as cell growth, differentiation, and immune response. Microtubules, in particular, play a crucial role in morphogenesis by governing the deposition of plant cell wall polysaccharides and, in consequence, the cell wall mechanics and cell shape. Scrutinizing the microtubule dynamics is therefore integral to understanding the spatiotemporal regulation of cellular activities. In this chapter, we outline steps to acquire 3D images of microtubules in epidermal pavement cells of Arabidopsis thaliana cotyledons using a confocal microscope. We introduce the steps to assess the microtubule distribution and organization using image processing software Bitplane Imaris and ImageJ. We also demonstrate how the interpretation of image material can be facilitated by post-processing with general-purpose image enhancement software using methods trained by artificial intelligence-based algorithms.

Plant phenotyping relevance

植物細胞の微小管分布・組織化を3D画像から取得・解析する技術プロトコルが中心であり、植物細胞の形態形成に関わる状態を画像処理で評価するため、方法論文として収録する。

Code and data availability

This is a methods protocol chapter describing confocal imaging of microtubules in Arabidopsis pavement cells and analysis with generic third-party software (ImageJ, Bitplane Imaris, Topaz Gigapixel AI). No public phenotype/trait datasets, author-deposited image data, analysis code, or trained models are mentioned; the仅

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