A Method for Estimating Fluorescence Emission Spectra from the Image Data of Plant Grain and Leaves Without a Spectrometer

Abstract

This study proposes a method for estimating the spectral images of fluorescence spectral distributions emitted from plant grains and leaves without using a spectrometer. We construct two types of multiband imaging systems with six channels, using ordinary off-the-shelf cameras and a UV light. A mobile phone camera is used to detect the fluorescence emission in the blue wavelength region of rice grains. For plant leaves, a small monochrome camera is used with additional optical filters to detect chlorophyll fluorescence in the red-to-far-red wavelength region. A ridge regression approach is used to obtain a reliable estimate of the spectral distribution of the fluorescence emission at each pixel point from the acquired image data. The spectral distributions can be estimated by optimally selecting the ridge parameter without statistically analyzing the fluorescence spectra. An algorithm for optimal parameter selection is developed using a cross-validation technique. In experiments using real rice grains and green leaves, the estimated fluorescence emission spectral distributions by the proposed method are compared to the direct measurements obtained with a spectroradiometer and the estimates obtained using the minimum norm estimation method. The estimated images of fluorescence emissions are presented for rice grains and green leaves. The reliability of the proposed estimation method is demonstrated.

Plant phenotyping relevance

植物の蛍光スペクトル画像を分光器なしで推定する撮像・計算法を開発し、分光放射計との比較で信頼性を検証しており、植物表現型取得法が中心です。

Code and data availability

The article describes multiband fluorescence imaging of rice grains and perilla leaves with ridge regression estimation, but contains no data availability statement, no public dataset or image deposit, and no author code repository. The only URL besides the license is a cited external reference (van Wieringen ridge-reg

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