Long wavelength near-infrared transmission spectroscopy of barley seeds using a supercontinuum laser: Prediction of mixed-linkage beta-glucan content.

Abstract

A supercontinuum laser was used to perform the first transmission measurements on intact seeds with long wavelength near-infrared spectroscopy. A total of 105 barley seeds from five different barley genotypes (Bomi, lys5.f, lys5.g, lys16 and lys95) were measured from 2275 to 2375 nm. The mixed-linkage (1→3,1→4)-β-D-glucan (BG) and protein content was measured with wet chemical analysis for each single seed. A partial least squares model correlated the BG % (w/w) with the spectral measurements with a R 2 CV and R 2 PRED of 0.83 and 0.90, respectively. The predictive model for BG could be improved by averaging spectra from the same seed and by replacing the individual seed BG content with the average BG of each barley genotype.

Plant phenotyping relevance

大麦種子のβ-グルカン含量という植物器官形質を、近赤外透過分光とPLSモデルで非破壊推定する手法の開発・検証が研究の中心である。

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