In vivo detection of salicylic acid in sunflower seedlings under salt stress.

Abstract

Salicylic acid (SA) is an important phytohormone. It plays an essential role in regulating many physiological processes of plants. Most of the conventional methods for SA detection are based on in vitro processes. More attention should be paid to develop in vivo methods for SA detection. In this work, Pt nanoflowers and GO were simultaneously electrodeposited and reduced on a Pt wire microelectrode in one step. The Pt nanoflowers/ERGO modified Pt microsensor demonstrated high sensitivity and selectivity for SA. SA could be detected from 100 pM to 1 μM with a detection limit of 48.11 pM. Then this microsensor was used to detect SA in the stem of sunflower seedlings under different salt stresses in vivo . The result showed that with the increasing concentration of salt, SA levels decreased. Our result was also confirmed by UPLC-MS and gene expression analysis. To the best of our knowledge, this is the first report of in vivo detection of SA in plants using the Pt nanoflowers/ERGO modified Pt microelectrode. It is foreseeable that our strategy could pave the way for the in vivo detection of phytohormones in plants.

Plant phenotyping relevance

植物体内の植物ホルモン濃度という生理状態を測定するマイクロセンサーを開発・性能評価し、ヒマワリで実証しており、測定法が研究の中心である。

Code and data availability

The paper describes an electrochemical microsensor for in vivo salicylic acid detection in sunflower seedlings. No public phenotype datasets, images, code, models, or paper-specific data deposits are mentioned; only an ESI supplement (RA-008-C8RA03475C-s001) is referenced, but no authors' public URL is provided and no-

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