that were missing, zero, or otherwise aberrant. It was also programmed to identify and remove inverted day-night cycle patterns, as well as values that were statistically insignificant. Figure 9 shows applications of data cleaning on the example dataset. For more details, please check codes that have been deposited on Zenodo ( https://doi.org/10.5281/zenodo.20080750 , D’Agostino, 2026 ). Figure 9. Example of data cleaning using the algorithm. Green is kept data and red is discarded data. Conclusion In summary, the present protocol is not confined to the descriptive monitoring of Ψ soil and Ψ leaf
Open resource ↗Zenodo · 10.5281/zenodo.20080750 · lines:452-504Unverified paper record
Protocols for in situ continuous monitoring of water relations/potential in soil and leaf.
Open research Europe · 29 May 2026 · 10.12688/openreseurope.21382.2
Abstract
Within the soil-plant-atmosphere continuum, water movement is driven by the water potential gradients between these three domains. To have a comprehensive understanding of such water relations, an examination of how plants respond to variations in soil water availability is required. The methodologies employed for measuring water potential in leaf (Ψ leaf ) and soil (Ψ soil ) have undergone a significant evolution; transitioning from qualitative assessments to the use of high-precision digital sensors over the past few decades. The present protocol aims to provide a comprehensive, step-by-step guide from the germination phase of maize and tomato plants to the installation of two sensors that continuously monitor water potential in the leaf (PSY1 psychrometer) and in the soil (TEROS 21 matric potential sensor). Additionally, we present the code for processing the raw data files in RStudio.
Plant phenotyping relevance
葉の水ポテンシャルを連続測定するセンサー設置、データ処理コード、手順を中心とした植物生理形質の測定プロトコルであり、方法論的貢献が明確。
abstractThe present protocol aims to provide a comprehensive, step-by-step guide from the germination phase of maize and tomato plants to the installation of two sensors that continuously monitor water potential in the leaf (PSY1 psychrometer) and in the soil (TEROS 21 matric potential sensor).
abstractAdditionally, we present the code for processing the raw data files in RStudio.
Code and data availability
The paper deposits its authors' R analysis notebook with an example water-potential dataset, the CR800 datalogger program, and an installation video on Zenodo, all publicly accessible.
(1) the address of each Teros 21; (2) the data transporting port (“C1” or “C3”); (3) the creation of dataset files to store the recorded soil matric potential and temperature, as well as the voltage of the battery for power supply; (4) the time interval for the data recording. An example of the program was deposited on Zenodo ( https://doi.org/10.5281/zenodo.17158115 ), with the document name of “Program-CR800”). Before starting, install the software of “Device Configuration Utility” and “PC400” from Campbell Scientific ( https://www.campbellsci.com/devconfig ; https://www.campbellsci.com/pc400 ). “CRBasic Editor” is integrated inside PC400. For more details about the programming, please re
Open resource ↗Zenodo · 10.5281/zenodo.17158115 · lines:321-378ulic limitation, soil-root disconnection, and recovery. Consequently, this linkage of the protocol to mechanistic analyses of water transport in the SPAC is more direct. Ethics and consent Ethical approval and consent were not required. Data availability The datasets and codes to analyze the data have been deposited on Zenodo ( https://doi.org/10.5281/zenodo.20080750 , D’Agostino (2026) ). Data are available under the terms of the Creative Commons Zero v1.0 Universal. An additional explicative video for the psychrometer installation on leaves is available on Zenodo ( https://doi.org/10.5281/zenodo.17510720 , Degand et al. (2025) ). The author(s) declare that this video is released under the
Open resource ↗Zenodo · 10.5281/zenodo.20080750 · lines:505-651This is an automatically classified, unverified record. Curator approval is required before any resource enters the Catalog.