Unverified paper record
Analysis of Complex Carbohydrate Composition in Plant Cell Wall Using Fourier Transform Mid-Infrared Spectroscopy.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2023 · 10.1007/978-1-0716-3151-5_15
Abstract
Fourier transform mid-infrared spectroscopy (FTIR) is a powerful tool for compositional analysis of plant cell walls. The infrared spectrum generates a fingerprint of a sample with absorption peaks corresponding to the frequency of vibrations between the bonds of the atoms making up the material. Here we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall. The FTIR method described here facilitates high-throughput identification of the major compositional differences across a large set of samples in a low-cost and non-destructive manner.
Plant phenotyping relevance
植物細胞壁の組成という植物形質を、FTIRとPCAで高スループット・非破壊に取得する方法が研究の中心であるため。
abstractHere we describe a method focused on the use of FTIR in combination with principal component analysis (PCA) to characterize the composition of the plant cell wall.
abstractThe FTIR method described here facilitates high-throughput identification of the major compositional differences across a large set of samples in a low-cost and non-destructive manner.
Code and data availability
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