Unverified paper record
Methods for Autophagy Detection by Fluorescence Microscopy.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2026 · 10.1007/978-1-0716-5300-5_20
Abstract
Fluorescence microscopy is pivotal for investigating autophagy's role in plant antiviral immunity. Here, we present a standardized procedure using complementary probes, CFP-ATG8f for autophagosomal structures and monodansylcadaverine (MDC) for autophagic vacuoles, to assess autophagy during viral infection. This combined CFP-ATG8f and MDC staining system provides a powerful, reproducible method for evaluating autophagic activity in plant-virus interactions.
Plant phenotyping relevance
植物のオートファジー活性という生理状態を蛍光顕微鏡と相補的プローブで測定する標準化・再現可能な手法が研究の中心であり、植物表現型測定法に該当する。
abstractHere, we present a standardized procedure using complementary probes, CFP-ATG8f for autophagosomal structures and monodansylcadaverine (MDC) for autophagic vacuoles, to assess autophagy during viral infection.
abstractThis combined CFP-ATG8f and MDC staining system provides a powerful, reproducible method for evaluating autophagic activity in plant-virus interactions.
Code and data availability
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