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Small-Sample Authenticity Identification and Variety Classification of Anoectochilus roxburghii (Wall.) Lindl. Using Hyperspectral Imaging and Machine Learning.

Plants (Basel, Switzerland) · 10 Apr 2025 · 10.3390/plants14081177

Abstract

This study aims to utilize hyperspectral imaging technology combined with machine learning methods for the authenticity identification and classification of Anoectochilus roxburghii and its counterfeit species. Hyperspectral data were collected from the front and back leaves of nine species of Goldthread and two counterfeit species (Bloodleaf and Spotted-leaf), followed by classification using a variety of machine learning models, including Support Vector Machine (SVM), K-Nearest Neighbors (KNN), Random Forest (RF), Linear Discriminant Analysis (LDA), and Convolutional Neural Networks (CNN). The experimental results demonstrated that the SVM model achieved 100% classification accuracy for distinguishing Goldthread from its counterfeit species, effectively capturing the spectral differences between the front and back leaves. In contrast, traditional machine learning models showed varied performance, with SVM proving superior due to its ability to handle high-dimensional feature spaces. The introduction of a multi-view spectral fusion CNN model, which integrates spectral data from both the front and back leaves, further enhanced classification accuracy, achieving a perfect classification rate of 100%. This approach highlights the potential of hyperspectral imaging and machine learning in plant authenticity identification and provides a new perspective for the detection of counterfeit species.

Plant phenotyping relevance

葉のハイパースペクトル画像から植物種・真正性を分類する取得・解析手法が研究の中心であり、植物の識別可能な状態を直接評価しているため。

abstractutilize hyperspectral imaging technology combined with machine learning methods for the authenticity identification and classification of Anoectochilus roxburghii and its counterfeit species
abstractThe introduction of a multi-view spectral fusion CNN model, which integrates spectral data from both the front and back leaves, further enhanced classification accuracy

Code and data availability

The supplied article blocks describe hyperspectral imaging of Anoectochilus roxburghii leaves and ML/CNN classification, but contain no data availability statement, no public dataset or image deposit, and no author code repository or URL. The hyperspectral data, images, and trained models are not stated as publicly可用,.

No evidence-backed public reproduction asset is currently recorded.

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