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Rapeseed Seedling Stand Counting and Seeding Performance Evaluation at Two Early Growth Stages Based on Unmanned Aerial Vehicle Imagery.

Frontiers in plant science · 21 Sept 2018 · 10.3389/fpls.2018.01362

Abstract

The development of unmanned aerial vehicles (UAVs) and image processing algorithms for field-based phenotyping offers a non-invasive and effective technology to obtain plant growth traits such as canopy cover and plant height in fields. Crop seedling stand count in early growth stages is important not only for determining plant emergence, but also for planning other related agronomic practices. The main objective of this research was to develop practical and rapid remote sensing methods for early growth stage stand counting to evaluate mechanically seeded rapeseed (Brassica napus L.) seedlings. Rapeseed was seeded in a field by three different seeding devices. A digital single-lens reflex camera was installed on an UAV platform to capture ultrahigh resolution RGB images at two growth stages when most rapeseed plants had at least two leaves. Rapeseed plant objects were segmented from images of vegetation indices using typical Otsu thresholding method. After segmentation, shape features such as area, length-width ratio and elliptic fit were extracted from the segmented rapeseed plant objects to establish regression models of seedling stand count. Three row characteristics (the coefficient of variation of row spacing uniformity, the error rate of the row spacing and the coefficient of variation of seedling uniformity) were further calculated for seeding performance evaluation after crop row detection. Results demonstrated that shape features had strong correlations with ground-measured seedling stand count. The regression models achieved R-squared values of 0.845 and 0.867, respectively, for the two growth stages. The mean absolute errors of total stand count were 9.79 and 5.11% for the two respective stages. A single model over these two stages had an R-squared value of 0.846, and the total number of rapeseed plants was also accurately estimated with an average relative error of 6.83%. Moreover, the calculated row characteristics were demonstrated to be useful in recognizing areas of failed germination possibly resulted from skipped or ineffective planting. In summary, this study developed practical UAV-based remote sensing methods and demonstrated the feasibility of using the methods for rapeseed seedling stand counting and mechanical seeding performance evaluation at early growth stages.

Plant phenotyping relevance

UAV画像からセグメンテーションと形状特徴抽出により、ナタネ幼苗の個体数・条列特性を推定する手法を開発し、精度検証しているため、植物表現型取得が中心です。

abstractThe main objective of this research was to develop practical and rapid remote sensing methods for early growth stage stand counting to evaluate mechanically seeded rapeseed (Brassica napus L.) seedlings.
abstractRapeseed plant objects were segmented from images of vegetation indices using typical Otsu thresholding method.
abstractAfter segmentation, shape features such as area, length-width ratio and elliptic fit were extracted from the segmented rapeseed plant objects to establish regression models of seedling stand count.
abstractResults demonstrated that shape features had strong correlations with ground-measured seedling stand count.

Code and data availability

The supplied blocks describe UAV imagery collection and image-processing methods (Pix4D, ERDAS, eCognition, SPSS, ArcMap Python scripts) but contain no public data deposit, no author code repository URL, and no availability statement for the UAV images, shape-feature datasets, or analysis scripts. The only URL present/

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