Water Spectral Patterns Reveals Similarities and Differences in Rice Germination and Induced Degenerated Callus Development.

Abstract

In vivo monitoring of rice ( Oryza sativa L.) seed germination and seedling growth under general conditions in closed Petri dishes containing agar base medium at room temperature (temperature = 24.5 ± 1 °C, relative humidity = 76 ± 7% (average ± standard deviation)), and induced degenerated callus formation with plant growth regulator, were performed using short-wavelength near-infrared spectroscopy and aquaphotomics over A period of 26 days. The results of spectral analysis suggest changes in water absorbances due to the production of common metabolites, as well as increases in biomass and the sizes of the samples. Quantitative models built to predict the day of the development provided better accuracy for rice seedlings growth compared to callus formation. Eight common water bands were identified as presenting prominent changes in the absorbance pattern. The water matrix of only rice seedlings showed three developmental stages: firstly expressing a predominantly weakly hydrogen-bonded state, then a more strongly hydrogen-bonded state, and then, again, a weakly hydrogen-bonded state at the end. In rice callus induction and proliferation, no similar change in water absorbance pattern was observed. The presented findings indicate the potential of aquaphotomics for the in vivo detection of degeneration in cell development.

Plant phenotyping relevance

近赤外分光とアクアフォトミクスを用いてイネの発芽・生長およびカルスの発達状態を経時的に推定し、定量モデルも構築しており、表現型取得手法が中心である。

Code and data availability

The paper reports NIR spectral phenotyping of rice seeds/callus (13,440 spectra) analyzed with PCA, PLSR, HCA, and aquagrams, but no public dataset, code, or model repository is provided. The Data Availability Statement explicitly restricts access: data are available only on request and 'not publicly available due to隐私

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