Assessing Plant Pigment Regulation in Circadian Experiments.

Abstract

Circadian rhythms affect many aspects of a plant's metabolism including, but not limited to, photosynthesis. Here, we provide a complete protocol for determining changes in the composition of photosynthetic pigments (chlorophyll and carotenoids), and we also consider its implementation within circadian experiments. We describe how to design a circadian experiment with the goal of assessing changes in pigment composition. We then perform two consecutive approaches to track changes in pigment composition: indirect noninvasive estimation of pigment composition (by reflectance or fluorescence) followed by direct pigment analysis (by chromatography or spectrophotometry). Finally, we present several considerations regarding data analyses.

Plant phenotyping relevance

植物の色素組成を反射・蛍光によって非破壊推定し、クロマトグラフィー等で検証する測定プロトコルが中心であり、植物の生理形質取得法に該当する。

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