Measurement of Arabidopsis thaliana Nuclear Size and Shape.

Abstract

Gene expression is tightly linked to the position of genes in the nucleus. Genomic regions associated with the nuclear envelope are usually repressed, including the heterochromatin carrying chromocenters. The shape and size of nuclei varies within tissues in plants and is dependent on proteins associated with the nuclear envelope. Here, we describe a protocol to isolate Arabidopsis thaliana nuclei and measure their size and morphology. Using this method, novel components regulating the nuclear envelope and chromatin association can be identified and analyzed.

Plant phenotyping relevance

植物核のサイズと形態を取得・測定するプロトコルが研究の中心であり、植物の形態的表現型を測定する方法論として収載対象です。

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