Genome-Wide Association Mapping of Root Traits in the Context of Plant Hormone Research.

Abstract

Genome-wide association (GWA) mapping is a powerful method for the identification of alleles that underlie quantitative traits. It enables one to understand how genetic variation translates into phenotypic variation. In particular, plant hormone signaling pathways play a key role in shaping phenotypes. This chapter presents a protocol for genome-wide association mapping of root traits of Arabidopsis thaliana in the context of hormone research. We describe a specific protocol for acquiring primary and lateral root trait data that is appropriate for GWA studies using FIJI (ImageJ), and subsequent GWA mapping using a user-friendly Internet application.

Plant phenotyping relevance

Arabidopsisの一次根・側根形質をFIJIで取得する具体的プロトコルが中心であり、GWA解析に用いる植物表現型取得法を提示している。

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