Protocol for evaluating seedling greening capacity during dark-to-light transition.

Abstract

We present a protocol to evaluate greening capacity in etiolated Arabidopsis seedlings during the critical dark-to-light transition. We describe steps for sample preparation and sowing and then detail procedures for quantifying protochlorophyllide accumulation in darkness, the greening rate upon illumination, and reactive oxygen species levels as an indicator of photo-oxidative stress. This protocol can be used for screening and phenotypic quantification across genetic backgrounds. For complete details of this protocol, please refer to Zhong et al. 1 and Zhong et al. 2 .

Plant phenotyping relevance

暗所から光への移行における植物の緑化能を定量するプロトコルで、表現型スクリーニングと遺伝背景間の定量が中心であるため。

Code and data availability

This is a methods protocol paper. The authors explicitly state 'This study did not generate or analyze datasets.' The only URLs in the key resources table (ZEISS ZEN, BioTek Gen5) are links to commercial, generic third-party software products, not authors' analysis code, datasets, images, or models. No paper-specific,公

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