A Near Infrared Fluorescent Nanosensor for Spatial and Dynamic Measurements of Auxin, Indole-3-Acetic Acid, in Planta

Abstract

Auxins, particularly indole-3-acetic acid (IAA), is a phytohormone critical for plant growth, development, and response to environmental stimuli. Despite its importance, there is a lack of species-independent sensors that allow direct and reversible detection of IAA. Herein, we introduce a novel near infrared fluorescent nanosensor for spatial and temporal measurement of IAA in planta using Corona Phase Molecular Recognition. The IAA nanosensor shows high specificity to IAA in vitro and was validated to localize and function in plant cells. The sensor works across different plant species without optimization and allows visualization of dynamic changes to IAA distribution and movement in leaf tissues. The results highlighted the utility of IAA nanosensor for understanding IAA dynamics in planta .

Plant phenotyping relevance

植物体内のIAAを空間・時間的に可視化する蛍光ナノセンサーを開発し、植物細胞で検証しており、植物生理状態の取得法が研究の中心です。

Code and data availability

The supplied blocks describe a CoPhMoRe-based near-infrared fluorescent nanosensor for IAA imaging in planta, including in vitro calibration, ratiometric leaf imaging, and a reaction-diffusion model. No blocks contain a data availability statement, public phenotype/trait dataset, deposited plant images or sensor data,或

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