Measurement of Arabidopsis thaliana Plant Traits Using the PHENOPSIS Phenotyping Platform.

Abstract

High-throughput phenotyping of plant traits is a powerful tool to further our understanding of plant growth and its underlying physiological, molecular, and genetic determinisms. This protocol describes the methodology of a standard phenotyping experiment in PHENOPSIS automated platform, which was engineered in INRA-LEPSE (https://www6.montpellier.inra.fr/lepse) and custom-made by Optimalog company. The seminal method was published by Granier et al. (2006). The platform is used to explore and test various ecophysiological hypotheses (Tisné et al. , 2010; Baerenfaller et al. , 2012; Vile et al. , 2012; Bac-Molenaar et al. , 2015; Rymaszewski et al. , 2017). Here, the focus concerns the preparation and management of experiments, as well as measurements of growth-related traits ( e.g ., projected rosette area, total leaf area and growth rate), water status-related traits ( e.g ., leaf dry matter content and relative water content), and plant architecture-related traits ( e.g ., stomatal density and index and lamina/petiole ratio). Briefly, a completely randomized (block) design is set up in the growth chamber. Next, the substrate is prepared, its initial water content is measured and pots are filled. Seeds are sown onto the soil surface and germinated prior to the experiment. After germination, soil watering and image (visible, infra-red, fluorescence) acquisition are planned by the user and performed by the automaton. Destructive measurements may be performed during the experiment. Data extraction from images and estimation of growth-related trait values involves semi-automated procedures and statistical processing.

Plant phenotyping relevance

PHENOPSIS自動表現型解析プラットフォームの実験・画像取得・形質抽出手順を体系的に記述しており、植物表現型取得法が中心である。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

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