Isotope discrimination of carbonyl sulfide ( 34 S) and carbon dioxide ( 13 C, 18 O) during plant uptake in flow-through chamber experiments

Abstract

Abstract. Carbonyl sulfide (COS) has been proposed as a proxy for gross primary production (GPP), as it is taken up by plants through a pathway comparable to that of CO2. COS diffuses into the leaf, where it undergoes an essentially one-way reaction in the mesophyll cells, irreversibly catalyzed by the enzyme carbonic anhydrase (CA), and is likely not respired by the leaf. In order to use COS as a proxy for GPP, the mechanisms of COS uptake and its coupling to photosynthesis need to be well understood. Characterizing the isotopic discrimination of COS during plant uptake could provide valuable information on the physiological COS uptake process and may help to constrain the COS budget. This study presents joint measurements of isotope discrimination during plant uptake for COS (CO34S) and CO2 (13CO2 and C18O16O). A C3 plant, sunflower (Helianthus annuus), and a C4 plant, papyrus (Cyperus papyrus), were enclosed in a flow-through plant chamber and exposed to varying light levels. The incoming and outgoing gas compositions were measured online, and discrete air samples were taken for isotope analysis. Simultaneously measuring fluxes and isotope discrimination of both COS and CO2 yielded a unique dataset that includes information on the plant's behavior and allowed for the estimation of stomatal- and mesophyll conductances. The average COS uptake fluxes were 73.3 ± 1.5 pmol m−2 s−1 for sunflower and 107.3 ± 1.5 pmol m−2 s−1 for papyrus (PAR > 0) and displayed virtually no trend with increasing PAR from 200 to 600 µmol m−2 s−1. The mean observed 34Δ for COS was 3.4 ± 1.0 ‰ for sunflower and 2.6 ± 1.0 ‰ for papyrus. 34Δ was stable across all light intensities, which could be explained by a sufficient stomatal opening and low variability in the ratio of mesophyll vs. ambient COS mole fraction, CmS/CaS. For both C3 and C4 plants, for CO2, a negative relationship was observed between the uptake flux and the isotopic discriminations 13Δ and 18Δ. The CO2 uptake and 13CO2 and C16O18O discriminations of sunflower have expected values for a C3 plant, while the low CO2 flux and high 13Δ and 18Δ values observed for papyrus were not in the typical C4 range, which was perhaps due to the relatively low light conditions during our experiments.

Plant phenotyping relevance

植物のCOS・CO2取り込み、同位体識別、気孔・葉肉コンダクタンスをフロースルー植物チャンバーで定量する生理的表現型測定が研究の中心であり、再利用可能な測定データセットと推定手法を提示している。

Code and data availability

The paper's isotope discrimination and gas-exchange dataset from the flow-through chamber experiments is publicly deposited on Zenodo by the authors.