Intelligent high-throughput recognition model for bitter gourd fruit morphology and tubercle characteristics.

Abstract

Bitter gourd, an important crop with both economic and medicinal value, requires precise identification of fruit shape and tubercle phenotypes to enhance breeding efficiency. To address the low efficiency and high subjectivity of traditional methods, this study proposes an improved YOLOv8-CEFC model for high-throughput automatic detection of the bitter gourd fruit shape and tubercle characteristics. First, the model integrates the ConvNeXt V2 module into the backbone network, combined with a Fully Convolutional Masked Autoencoder (FCMAE) framework and Global Response Normalization (GRN) layers to enhance feature extraction capabilities. Second, an Efficient Multi-scale Attention (EMA) mechanism is introduced, capturing local tubercle textures and global fruit shape contours simultaneously through a parallel dual-branch structure, while also improving the model’s robustness against cluttered backgrounds and environmental noise. Finally, Focal-CIoU Loss is incorporated to replace CIoU Loss, reducing the impact of class imbalance on model accuracy. The results show that the model achieves precision, recall, mAP50, mAP50-95, and F1 scores of 93.9%, 94.4%, 96.3%, 93.6%, and 94.15%, respectively, which represent improvements of 2.0%, 3.5%, 1.1%, 3.4%, and 2.75% compared to the original YOLOv8n model. The performance gain of the model was further examined using the bootstrap method, which confirmed that the improvement is statistically significant. Further validation through confusion matrix analysis, PR curves, and ablation experiments confirms the effectiveness of the improvements. Compared to other mainstream YOLO models, YOLOv8-CEFC demonstrates more accurate identification, better stability, and higher detection efficiency. The proposed improved YOLOv8-CEFC model provides an efficient solution for phenotypic analysis in Bitter Gourd breeding and holds significant importance for advancing the intelligentization of crop breeding.

Plant phenotyping relevance

苦瓜果実形状とこぶ形質を高スループットに自動認識するYOLOv8改良モデルを開発・検証しており、植物表現型の取得・抽出手法が研究の中心である。

Code and data availability

The paper's bitter gourd image dataset (1,716 original images, 5,190 augmented, 13,163 annotated samples) and YOLOv8-CEFC model are not publicly deposited; the Data Availability Statement states they are available from the corresponding author on reasonable request. No public URL or repository for the dataset or code/​

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