Unverified paper record
Ultrastructural Analysis and Three-Dimensional Reconstruction of Plasmodesmata.
Methods in molecular biology (Clifton, N.J.) · 1 Jan 2022 · 10.1007/978-1-0716-2132-5_4
Abstract
Array tomography (AT) is a new high-throughput imaging method for high-resolution imaging of ultrastructure and for 3-D reconstruction of cells and organelles. Here, we describe the entire procedure for obtaining a spatial image of the distribution of plasmodesmata (PD). As example, the protocol is applied here to reconstruct the number and arrangement of PD between cells undergoing differentiation during Arabidopsis somatic embryogenesis.
Plant phenotyping relevance
植物細胞のプラズモデスマの空間分布を取得・3D再構成する高スループット画像法と手順が中心であり、細胞構造という植物状態の測定に該当する。
abstractArray tomography (AT) is a new high-throughput imaging method for high-resolution imaging of ultrastructure and for 3-D reconstruction of cells and organelles.
abstractHere, we describe the entire procedure for obtaining a spatial image of the distribution of plasmodesmata (PD).
Code and data availability
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