Protocol for Producing Three-Dimensional Infrared Video of Freezing in Plants.

Abstract

Freezing in plants can be monitored using infrared (IR) thermography, because when water freezes, it gives off heat. However, problems with color contrast make 2-dimensions (2D) infrared images somewhat difficult to interpret. Viewing an IR image or the video of plants freezing in 3 dimensions (3D) would allow a more accurate identification of sites for ice nucleation as well as the progression of freezing. In this paper, we demonstrate a relatively simple means to produce a 3D infrared video of a strawberry plant freezing. Strawberry is an economically important crop that is subjected to unexpected spring freeze events in many areas of the world. An accurate understanding of the freezing in strawberry will provide both breeders and growers with more economical ways to prevent any damage to plants during freezing conditions. The technique involves a positioning of two IR cameras at slightly different angles to film the strawberry freezing. The two video streams will be precisely synchronized using a screen capture software that records both cameras simultaneously. The recordings will then be imported into the imaging software and processed using an anaglyph technique. Using red-blue glasses, the 3D video will make it easier to determine the precise site of ice nucleation on leaf surfaces.

Plant phenotyping relevance

植物の凍結部位・進行を可視化する3D赤外線動画の取得・処理手法を開発・実証しており、植物状態の計測法が中心である。

Code and data availability

The article is a JoVE protocol for 3D infrared video of plant freezing. It references Supplemental Videos 1 and 2 (anaglyph IR videos of strawberry and wheat freezing) and figures, but no public repository, dataset deposit, or author analysis code URL is provided anywhere in the supplied text. allowed_urls is empty, so

No evidence-backed public reproduction asset is currently recorded.