Detection and Seasonal Variations of Huanglongbing Disease in Navel Orange Trees Using Direct Ionization Mass Spectrometry.

Abstract

Citrus greening disease [Huanglongbing (HLB)] is the most destructive disease of citrus. In this work, we have established a metabolite-based mass spectrometry (MS) method for rapid detection of HLB in navel orange trees. Without sample pretreatment, characteristic mass spectra can be directly obtained from the raw plant samples using the direct MS method. The whole detection process can be accomplished within 1 min. By monitoring and comparisons of the healthy and infected plants throughout a whole year, characteristic MS peaks of metabolites are found to be specific responses from infected plants and, thus, could be used as biomarkers for detection of HLB. Therefore, HLB could be directly detected in the asymptomatic samples, such as stems, using this metabolite-based direct MS method. In addition, principal component analysis and partial least squares discriminant analysis modes of metabolites from healthy and infected trees were established for investigating differentiation and seasonal variations of HLB in leaves, veins, and stems, providing valuable information for understanding the HLB in different seasons.

Plant phenotyping relevance

HLBという植物の疾病状態を、前処理不要の直接質量分析と統計解析で検出する方法を開発・検証しており、病徴の偶発的測定ではなく植物状態の取得法が中心である。

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