Non-destructive measurement of soluble solids content of three melon cultivars using portable visible/near infrared spectroscopy

Abstract

In this study, a non-destructive method using visible/near infrared (Vis/NIR) spectroscopy was investigated to predict the soluble solids content (SSC) of intact melons (Cucumis melo L.) cv. ‘Manao’, ‘Jinhongbao’, ‘Xizhoumi’. A set of 360 samples (120 melons of each cultivar) was used to develop the calibration model, and two location (stylar-end and equatorial locations) models were investigated independently. The samples' spectra were obtained by a portable Vis/NIR photo-diode array spectrometer operated in reflectance mode. Multiplicative scatter correction (MSC), first derivative and Savizky-Golay (SG) smoothing in turn were applied to the obtained spectra. The region from 750 to 950 nm was selected to develop NIR models combined with the partial least squares (PLS) regression method. The results indicated that the stylar-end of the intact melon was the proper location to evaluate the SSC in the intact melon due to its suitable and exclusive physiological structure. A competitive adaptive reweighted sampling (CARS) algorithm was used to select effective wavelengths. Results showed that the CARS algorithm had great potential for simplifying the variables. Furthermore, another 195 samples were used for external prediction to evaluate the CARS-PLS model's accuracy and stability, which resulted in a high determination coefficient (R2p = 0.83) and a low root mean square error (RMSEP = 0.73 ºBrix).

Plant phenotyping relevance

携帯型Vis/NIR分光法で intact melon の可溶性固形分を非破壊推定する校正モデルを開発し、外部予測で精度・安定性を検証しており、植物器官の形質取得法が中心である。

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