"Light-Up" Near-Infrared Fluorescent Probe for Visualization of Hydrogen Sulfide Content and Abiotic Stress Response in Plants.

Abstract

Hydrogen sulfide (H 2 S) plays a vital role in plant physiology and stress adaptation, but the detection of endogenous H 2 S remains a challenge. In this work, a near-infrared fluorescent probe (NIR-BOD-HS) was synthesized using boron-dipyrromethene (BODIPY) as the raw material, which showed a good linear relationship in the concentration range of 0.1-70 μM and a detection limit of 56 nM. The long-wavelength emission (712 nm) reduced the interference of plant autofluorescence and improved the imaging quality. The probe combined with fluorescence imaging technology nondestructively realized the spatiotemporal distribution signal of H 2 S in the deep tissues of plants. In addition, the dynamic changes of H 2 S content during seed germination and seedling growth under abiotic stress were also demonstrated through the changes in fluorescence signals. This study helps to understand the physiological response mechanism of plants under abiotic stress and provides a scientific basis for further research on plant imaging and agricultural production.

Plant phenotyping relevance

植物体内H₂Sの時空間分布を非破壊的に可視化・定量する近赤外蛍光プローブと画像化手法が研究の中心であり、植物の生理状態を測定するフェノタイピング手法に該当する。

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