Characterization of Volatile Organic Compounds of Healthy and Huanglongbing-Infected Navel Orange and Pomelo Leaves by HS-GC-IMS.

Abstract

The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama, is the only natural vector of bacteria responsible for Huanglongbing (HLB), a worldwide destructive disease of citrus. ACP reproduces and develops only on the young leaves of its rutaceous host plants. Olfactory stimuli emitted by young leaves may play an important role in ACP control and HLB detection. In this study, volatile organic compounds (VOCs) from healthy and HLB-infected young leaves of navel orange and pomelo were analyzed by headspace-gas chromatography-ion mobility spectrometry (HS-GC-IMS). A total of 36 compounds (including dimers or polymers) were identified and quantified from orange and 10 from pomelo leaves. Some compounds showed significant differences in signal intensity between healthy and HLB-infected leaves and may constitute possible indicators for HLB infection. Principal component analysis (PCA) clearly discriminated healthy and HLB-infected leaves in both orange and pomelo. HS-GC-IMS was an effective method to identify VOCs from leaves. This study may help develop new methods for detection of HLB or find new attractants or repellents of ACP for prevention of HLB.

Plant phenotyping relevance

HS-GC-IMSによる葉の揮発性成分測定とPCA分類を用いて、HLB感染状態の検出可能性を評価しており、植物の病害状態を抽出する測定手法が中心です。

Code and data availability

The paper reports HS-GC-IMS VOC measurements of citrus leaves, but no public phenotype dataset, raw spectra, images, analysis code, or model deposit is mentioned. Analysis used proprietary software (LAV, Gallery Plot, SPSS). Leaf samples are only 'available from the authors,' which does not meet the public-asset bar; a

No evidence-backed public reproduction asset is currently recorded.