Protocol to analyse the structural composition by fluorescence microscopy and different conventional and fluorescence staining methods.

Abstract

The protocol shows the effectiveness of using safranin-fast green stain for fluorescence microscopy. This staining technique has been used in conventional microscopy to perform anatomical characterizations of plants. However, this protocol describes the procedure for using samples stained with safranin-fast green in conjunction with fluorescence microscopy. The strength of the protocol lies in the fact that the samples are permanent and allows for effective differentiation of lignified and cellulosic walls unlike conventional fluorescence microscopy stains such as Congo red-acridine orange, calcofluor, and autofluorescence. The protocol for making fluorescence intensity measurements is also standardized, allowing the data to be used for statistical analysis and inference about the chemical composition of plant cell walls.

Plant phenotyping relevance

植物細胞壁のリグニン化・セルロース性を蛍光顕微鏡と標準化した蛍光強度測定で評価するプロトコルが研究の中心であり、植物組織の構造・化学的形質を取得する方法開発に該当する。

Code and data availability

This is a methods/protocol paper describing staining and fluorescence microscopy procedures. It mentions supplementary files (ImageJ step-by-step instructions) but provides no public URL or deposit for them, and states 'No data was used for the research described in the article.' No public phenotype datasets, images,作者

No evidence-backed public reproduction asset is currently recorded.