Quantitative Analysis of UV-B Radiation Interception in 3D Plant Structures and Intraindividual Distribution of Phenolic Contents

Abstract

Ultraviolet-B (UV-B) acts as a regulatory stimulus, inducing the dose-dependent biosynthesis of phenolic compounds such as flavonoids at the leaf level. However, the heterogeneity of biosynthesis activation generated within a whole plant is not fully understood until now and cannot be interpreted without quantification of UV-B radiation interception. In this study, we analyzed the spatial UV-B radiation interception of kales (Brassica oleracea L. var. Acephala) grown under supplemental UV-B LED using ray-tracing simulation with 3-dimension-scanned models and leaf optical properties. The UV-B-induced phenolic compounds and flavonoids accumulated more, with higher UV-B interception and younger leaves. To distinguish the effects of UV-B energy and leaf developmental age, the contents were regressed separately and simultaneously. The effect of intercepted UV-B on flavonoid content was 4.9-fold that of leaf age, but the effects on phenolic compound biosynthesis were similar. This study confirmed the feasibility and relevance of UV-B radiation interception analysis and paves the way to explore the physical and physiological base determining the intraindividual distribution of phenolic compound in controlled environments.

Plant phenotyping relevance

3次元スキャン植物モデルとレイトレーシングにより、植物体内のUV-B放射遮蔽・分布を定量化する手法を中心に検証しており、植物構造に基づく生理状態の測定法として適格です。

Code and data availability

The article describes 3D-scanned kale models, ray-tracing simulations, and phenolic measurements, but no public dataset, image, code, or model repository is provided. The Data Availability Statement states the data is contained within the article only.

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