Laser biospeckles as a speedy tool to investigate the effects of sound on lentil (Lens esculenta puyensis) seeds.

Abstract

Objective We propose the application of laser biospeckles-a non-destructive, non-contact, real-time technique-to investigate the effect of sound pressure levels and different frequency sounds on lentil (Lens esculenta puyensis) seeds. Lentil seeds were illuminated by a laser diode source with a wavelength of 630 nm, and biospeckle patterns resulting from the interference of scattered laser light were captured as time-sequenced image frames. Biospeckles were recorded under white noise as well as under different frequencies of 100 Hz, 1 kHz, and 10 kHz at 80 dB. The time-sequenced frames were analyzed for the effects of sound by calculating the correlation between the frames and were characterized by a parameter called BA (Biospeckle Activity), which reflects the level of internal activity within the seeds. Results We found that the BA value was lower under white noise, indicating reduced activity within the seeds. Furthermore, a clear dependence of BA on sound frequency was observed, with this trend becoming apparent after six hours-well before visible germination began. It was found that, depending on the frequency applied, lentil seed germination could either be accelerated (e.g., at 1 kHz) or decelerated (e.g., at 100 Hz or 10 kHz). These results suggest that the laser speckle method may enable faster characterization of the characteristic frequency response of different plant seed species.

Plant phenotyping relevance

レーザーバイオスペックルによる非破壊画像計測とBA指標で種子内部活動・発芽応答を抽出する手法が研究の中心であり、植物フェノタイピング手法の応用・開発に該当する。

Code and data availability

The paper reports laser biospeckle imaging of lentil seeds under sound exposure, but states 'No datasets were generated or analysed during the current study.' No public phenotype data, biospeckle images/videos, MATLAB analysis code, or trained models are deposited. The supplementary material is a 155.8KB docx of (perin

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