Effect of cadmium stress on certain physiological parameters, antioxidative enzyme activities and biophoton emission of leaves in barley (Hordeum vulgare L.) seedlings.

Abstract

Biophoton emission is a well-known phenomenon in living organisms, including plant species; however, the underlying mechanisms are not yet well elucidated. Nevertheless, non-invasive stress detection is of high importance when in plant production and plant research. Therefore, the aim of our work was to investigate, whether biophoton emission is suitable for the detection of cadmium stress in the early phase of stress evolution and to identify certain stress-related events that occur rapidly upon cadmium exposure of barley seedlings parallel to biophoton emission measurements. Changes of biophoton emission, chlorophyll content estimation index, ascorbate level, the activity of ascorbate- and guaiacol peroxidase enzymes and lipid oxidation were measured during seven days of cadmium treatment in barley (Hordeum vulgare L.) seedlings. The results indicate that the antioxidant enzyme system responded the most rapidly to the stress caused by cadmium and the lipid oxidation-related emission of photons was detected in cadmium-treated samples as early as one day after cadmium exposure. Furthermore, a concentration-dependent increase in biophoton emission signals indicating an increased rate of antioxidative enzymes and lipid oxidation was also possible to determine. Our work shows evidence that biophoton emission is suitable to identify the initial phase of cadmium stress effectively and non-invasively.

Plant phenotyping relevance

植物のカドミウムストレスを非侵襲的な生物フォトン発光で検出する手法の適用可能性を中心に評価しており、単なる生理測定ではなくストレス状態の表現型取得法が主要な貢献です。

Code and data availability

The paper's phenotype measurements (BPE, SPAD, enzyme activities, MDA, ascorbate) are stated to be in Supporting information files (S1 Data, S2 Data XLSX; S1 File ZIP), but no public URL for these files is provided among the allowed URLs. The only URL present (http://acta.bibl.u-szeged.hu/22528/) belongs to a cited 200

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