Rapid quantitative characterization of tea seedlings under lead-containing aerosol particles stress using Vis-NIR spectra.

Abstract

The problem of excessive lead content in tea has become more and more serious with the development of society and industry. This paper investigated the ability of visible and near-infrared (Vis-NIR) spectroscopy to evaluate foliar lead uptake by tea plants through simulating real air pollution. Lead content of tea leaves in different treatment groups during stress time was measured by inductively coupled plasma mass spectrometry (ICP-MS). It was determined that stomata can be a channel for lead particles in the air and most of the lead entering through the stomata accumulates in the leaves. The spectral variation of treated samples was measured, and it was found that a combination of partial least squares-discriminant analysis (PLS-DA) and spectral responses can perfectly classify the tea samples under different lead concentrations stress with an overall accuracy of 0.979. Then the Vis-NIR spectra were used for fast monitoring physiological and biochemical indicators in tea leaves under atmospheric deposition. Relevant spectra pretreatment methods and characteristic wavelength selection approaches were evaluated for quantitative analysis and then optimal prediction models to instantly detect quality indicators in tea samples were built. Among predictive models, PLS had the best results (RMSE = 0.139 mg/g, 0.663 mmol/g, and 1.494 μmol/g) for the prediction of chlorophyll a (Chl-a), ascorbic acid (ASA), and glutathione (GSH), respectively. Also, principal component regression (PCR) gave the best results (RMSE = 0.053 mg/g, 0.024 mg/g, and 0.011%) for prediction of chlorophyll b (Chl-b), carotenoid (Car) and moisture content (MC), respectively. Results of this study can be applied for developing an effective and reliable approach for monitoring atmospheric deposition in plants.

Plant phenotyping relevance

茶葉の葉面鉛蓄積、ストレス状態、クロロフィル等の植物形質をVis-NIR分光で分類・定量する手法を評価し、前処理・波長選択・予測モデルも比較しており、フェノタイピング手法が中心である。

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