Ultrasensitive molecular imprinted electrochemical sensor for in vivo determination of glycine betaine in plants.

Abstract

Glycine betaine (GB) is a bioactive molecule protecting plants from abiotic stress. This study fabricated an ultrasensitive molecular imprinted polymer (MIP) electrochemical sensor to perform in vivo measurements of GB. Polydopamine (PDA) was formed on the carboxylated multi-walled carbon nanotubes (COOH-MWCNTs) by spontaneous polymerisation of dopamine (DA). Then MIP-coated MWCNTs were fabricated on a Au nanoparticles (NP) and thionine (Thi) modified screen-printed electrode (SPE). The MIP-COOH-MWCNTs/pThi/AuNPs/SPE exhibited an ultrasensitive GB detection response between 1 fmol/L and 10 mmol/L (R 2 = 0.996) with a low detection limit (0.707 fmol/L, S/N = 3). In vivo measurement of GB in cucumber seedling leaves under different salinity stress conditions confirmed the practical applicability of the MIP sensor. Thus, this study proposed a novel and promising fabrication method for an electrochemical MIP sensor that has broad application prospects in precision agriculture.

Plant phenotyping relevance

植物体内のグリシンベタインという生理状態を測定する電気化学センサーの作製・性能評価が研究の中心であり、植物フェノタイピング手法に該当する。

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