Protocol for evaluating salt and alkaline tolerance in maize seedlings using a sand-bed germination assay.

Abstract

Here, we present a protocol to independently and concurrently evaluate salt and alkaline tolerance in maize at the seedling stage using a controlled sand-bed germination assay. We describe steps for preparing standardized stress solutions, establishing the sand-bed assay, applying stresses either independently or in combination, and measuring multiple phenotypic traits. Furthermore, we detail procedures for ranking germplasm using a comprehensive scoring system based on a membership function. This protocol enables reproducible, large-scale screening of maize germplasm at the seedling stage.

Plant phenotyping relevance

幼苗耐盐碱性状の再現可能な大規模フェノタイピング用アッセイとスコアリング手順を中心に提示しており、単なる生物学的処理実験ではない。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

No evidence-backed public reproduction asset is currently recorded.