dual author(s) and contributor(s) and not of MDPI and/or the editor(s). MDPI and/or the editor(s) disclaim responsibility for any injury to people or property resulting from any ideas, methods, instructions or products referred to in the content. Supplementary Materials The following supporting information can be downloaded at: https://www.mdpi.com/article/10.3390/plants12183278/s1 , Table S1: Average daily lesion length results from four repeated experiments screening the efficacy of products against B. cinerea .; Table S2: Detached leaf assay results for four repeated experiments screening the efficacy of products with amended pH against B. cinerea . Click here for additional data file. Au
Open resource ↗lines:67-140Unverified paper record
Using Detached Industrial Hemp Leaf Inoculation Assays to Screen for Varietal Susceptibility and Product Efficacy on Botrytis cinerea .
Plants (Basel, Switzerland) · 15 Sept 2023 · 10.3390/plants12183278
Abstract
In greenhouse production, grey mould caused by Botrytis cinerea Pers. is one of the most widespread and damaging diseases affecting medicinal cannabis (MC). Fungicide options to control this disease are extremely limited due to the regulations surrounding fungicides and chemical residues as the product end users are medical patients, often with compromised immune systems. Screening for alternative disease control options, such as biological and organic products, can be time-consuming and costly. Here, we optimise and validate a detached leaf assay as a quick and non-destructive method to evaluate interactions between plants and pathogens, allowing the assessment of potential pathogens' infectivity and product efficacy. We tested eight industrial hemp varieties for susceptibility to B. cinerea infection. Using detached leaves from a susceptible variety, we screened a variety of chemical or organic products for efficacy in controlling the lesion development caused by B. cinerea . A consistent reduction in lesion growth was observed using treatments containing Tau-fluvalinate and Myclobutanil, as well as the softer chemical alternatives containing potassium salts. The performance of treatments was pH-dependent, emphasizing the importance of applying them at optimal pH levels to maximise their effectiveness. The detached leaf assay differentiated varietal susceptibility and was an effective method for screening treatment options for diseases caused by Botrytis. The results from the detached leaf assays gave comparable results to responses tested on whole plants.
Plant phenotyping relevance
ボトリティス感染による葉の病斑進展を測定するデタッチドリーフアッセイを最適化・検証し、品種感受性と製品効果のスクリーニングに用いているため、植物病害状態の取得手法が中心です。
abstractHere, we optimise and validate a detached leaf assay as a quick and non-destructive method to evaluate interactions between plants and pathogens, allowing the assessment of potential pathogens' infectivity and product efficacy.
abstractThe detached leaf assay differentiated varietal susceptibility and was an effective method for screening treatment options for diseases caused by Botrytis.
Code and data availability
The paper's detached leaf assay lesion measurements are contained in the manuscript and its Supplementary Materials (Tables S1 and S2 with average daily lesion length data), which are publicly downloadable from the MDPI article's supplementary file URL. No author analysis code or trained models are mentioned.
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