Near-infrared fluorescent probe to track Cys in plant roots under heavy metal hazards and its application in cells and zebrafish.

Abstract

Heavy metals, including Hg 2+ , Cr 6+ and Cd 2+ , have always been a major issue in environmental pollution, leading to abnormal changes in the levels of biologically active molecules including Cys in plants, seriously affecting all aspects of the growth and development of plants. This makes it essential to develop a simple and practical method to study the potential impact of heavy metals on plants. In this paper, our research group has developed near-infrared fluorescent probe WRM-S, which has the advantages of fast response, sensitivity to Cys, and successfully applying it to cells and zebrafish. Moreover, it combined the close relationship between heavy metal stress on plants and Cys, using Cys as the detection target, monitoring the internal environment changes of two plants under Hg 2+ , Cr 6+ , and Cd 2+ stress in the environment, and then conducting 3D imaging. The results indicated that the probe has strong penetration ability in plant tissues, and revealed abnormal changes in plant Cys levels caused by heavy metal stress-induced cellular oxidative stress or cytotoxicity. Thus, the in-situ imaging detection of this probe provides a direction for the physiological dynamics research of plant environmental stress.

Plant phenotyping relevance

植物組織内のCys変動を可視化・定量する近赤外蛍光プローブを開発し、重金属ストレス下の植物生理状態を3Dイメージングする方法が研究の中心である。

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