Mass Spectrometry Imaging for Spatial Chemical Profiling of Vegetative Parts of Plants.

Abstract

The detection of chemical species and understanding their respective localisations in tissues have important implications in plant science. The conventional methods for imaging spatial localisation of chemical species are often restricted by the number of species that can be identified and is mostly done in a targeted manner. Mass spectrometry imaging combines the ability of traditional mass spectrometry to detect numerous chemical species in a sample with their spatial localisation information by analysing the specimen in a 2D manner. This article details the popular mass spectrometry imaging methodologies which are widely pursued along with their respective sample preparation and the data analysis methods that are commonly used. We also review the advancements through the years in the usage of the technique for the spatial profiling of endogenous metabolites, detection of xenobiotic agrochemicals and disease detection in plants. As an actively pursued area of research, we also address the hurdles in the analysis of plant tissues, the future scopes and an integrated approach to analyse samples combining different mass spectrometry imaging methods to obtain the most information from a sample of interest.

Plant phenotyping relevance

植物組織の化学成分の空間分布を測定する質量分析イメージング手法を中心に、試料調製・データ解析・植物での応用と課題をレビューしており、植物フェノタイピング手法の方法論的レビューに該当する。

Code and data availability

This is a review article on mass spectrometry imaging in plants. It reports no original phenotyping measurements, datasets, images, or author analysis code. The Data Availability Statement reads 'Not applicable.' Software mentioned (SCiLS, BioMap, MSiReader) are generic third-party tools, not authors' assets, and allDO

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