PLANT MICROTECHNIQUE WITH RESIN - TOWARDS PLANT HISTOLOMICS

Abstract

ABSTRACT Plant microtechnique is a sequence of skill-intensive histological and microscopy procedures that often yield limited quantitative information. However, it provides the cellular context needed to uncover biomolecular functions. In this work, we developed an easier microtechnique and a novel histolomic approach for the quantitative analysis of histological features. We replaced paraffin with resin as the embedding medium, developed an adhesive treatment for glass slides, and developed a trichrome staining. These improvements provided superior tissue stability and greatly facilitated the skill-dependent steps. Unlike current stainings, our trichrome staining produced a broader color palette and sharply contrasted numerous organelles and ultrastructures in light microscopy. We leveraged these microtechnique advances through image segmentation and quantitative analysis in MATLAB and Adobe Photoshop to measure a wide range of morphometric and compositional features, thereby generating the histolome. To validate this workflow, we applied it comprehensively and systematically to several model plants and calculated their C 4 Kranz-anatomy level using a combination of characteristic histological features. The histolomes provided new insights into cellular functions and quantitative anatomical differentiation among species. The resin-based microtechnique and histolomic approach will help facilitate, standardize, and make plant histology research quantitative. GRAPHICAL ABSTRACT

Plant phenotyping relevance

樹脂包埋・染色・画像セグメンテーション・定量解析を統合し、植物組織の形態・構成特徴を抽出する新規ヒストロミクス手法を開発・検証しており、植物表現型取得が中心です。

Code and data availability

植物フェノタイピング解析を再現する公開資産であることを、入力本文と直接リンクから確認できなかったため保留しました。

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