Efficient and accurate tiller counting of hand-collected samples using images of straw bundles.

Abstract

We present a novel method for accurately counting winter wheat tillers based on RGB images from hand-collected samples. An efficient sample preparation method assembles wheat tillers into bundles from which individual tillers are robustly detected automatically, using classical image analysis. A custom-made user interface ('TillerCounter' program) allows adjusting the automatic detections interactively, which leads to highly accurate tiller counts comparable to the ground truth obtained by manual counting. The key contributions of our work include:1.An efficient method for imaging straw tillers based on bundle assembly.2.An extensive study of the obtained image quality and comparison with the ground truth data from manual counting.3.Demonstration of the approach's high accuracy using correlation analysis (Pearson correlation coefficient R = 0.973 compared to ground truth) and error analysis (root mean squared relative errors below 5 %).

Plant phenotyping relevance

小麦分げつ数という植物形態形質を、画像取得・古典的画像解析・専用ソフトウェアで自動推定し、手動計数を基準に精度検証しているため、フェノタイピング手法が中心です。

Code and data availability

The paper's authors publicly released the TillerCounter GUI source code on GitHub, which implements the Hough-transform-based tiller counting analysis used in this study. The paper also cites original image/count data at Zenodo (10.5281/zenodo.14446564), but no Zenodo URL is present in the allowed URL list, so only the